Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,819,098	G→A	intergenic (+37198/+16286)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,819,098	0	G	A	100.0%	205.3 / NA	73	intergenic (+37198/+16286)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/40);  total (33/40)

TAAATAAGAGTAAAGACCTTTTTCATCTTTCAGTAATTCCTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATAATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACAGTACGATTTTGGCAAATTTTATGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCTTGGGTTATTGACTAGTGCCCTTGCTATCGCGATCCGTTGACGTTGTCCTCCGGACAAACCGGCTCCCTGTTCCCCCACAACCGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCTCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGGCATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAAGACGACACCGACTTGCCGGCGCAACCAGTTT  >  1/1818853‑1819342                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     tAAATAATAGTAAAGGCCTTTTTCATCTTTCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGccc                                                                                                                                                                                                                                                 <  1:562634/251‑1 (MQ=255)     taatagTAAAGGCCTTTTTCATCTTTCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTg                                                                                                                                                                                                                                              >  1:758391/5‑250 (MQ=255)      aatagTAAAGGCCTTTTTCATCTTTCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCTTCTCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTg                                                                                                                                                                                                                                              <  1:681537/248‑1 (MQ=255)         agTAAAGGCCTTTTTCATCTTTCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGGGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTAt                                                                                                                                                                                                                                          >  1:187141/1‑250 (MQ=255)                gccTTTTTCATCTTTCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATc                                                                                                                                                                                                                                   <  1:120910/249‑1 (MQ=255)                         aTCTTTCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGtt                                                                                                                                                                                                                         >  1:51108/1‑251 (MQ=255)                                    aTTCTTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGAc                                                                                                                                                                                                              <  1:160093/251‑1 (MQ=255)                                    aTTCTTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGAc                                                                                                                                                                                                              <  1:45077/251‑1 (MQ=255)                                     ttcttGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGccc                                                                                                                                                                                                                                                 >  1:873262/5‑215 (MQ=255)                                     ttcttGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGAc                                                                                                                                                                                                              <  1:115218/246‑1 (MQ=255)                                            tGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCgg                                                                                                                                                                                                       >  1:449218/1‑250 (MQ=255)                                                   cTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGt                                                                                                                                                                                               <  1:103443/251‑1 (MQ=255)                                                     tGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTc                                                                                                                                                                                             <  1:245272/251‑1 (MQ=255)                                                             gatTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACaa                                                                                                                                                                                     >  1:275982/1‑251 (MQ=255)                                                               tttCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAAca                                                                                                                                                                                   >  1:412044/1‑250 (MQ=255)                                                                ttCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAAcag                                                                                                                                                                                  <  1:484118/251‑3 (MQ=255)                                                                   aCCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAgtgt                                                                                                                                                                               <  1:590432/251‑1 (MQ=255)                                                                     cTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTta                                                                                                                                                                             <  1:300699/251‑1 (MQ=255)                                                                      tttGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTtat                                                                                                                                                                            <  1:14757/251‑1 (MQ=255)                                                                          tCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAAcc                                                                                                                                                                        <  1:810527/251‑1 (MQ=255)                                                                          tCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAAc                                                                                                                                                                         <  1:189648/250‑1 (MQ=255)                                                                                acaATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAAc                                                                                                                                                                                    <  1:285579/233‑1 (MQ=255)                                                                                acaATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAAcc                                                                                                                                                                        <  1:98481/245‑1 (MQ=255)                                                                                   atgatGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAAc                                                                                                                                                                         >  1:43073/4‑241 (MQ=255)                                                                                   atgatGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTaa                                                                                                                                                               >  1:444671/4‑251 (MQ=255)                                                                                    tgatGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAAt                                                                                                                                                              <  1:179954/249‑1 (MQ=255)                                                                                         cGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAg                                                                                                                                                          <  1:727956/250‑1 (MQ=255)                                                                                                 aTTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAAt                                                                                                                                                              >  1:34838/1‑238 (MQ=255)                                                                                                         cAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTgcgcg                                                                                                                                         >  1:122206/1‑250 (MQ=255)                                                                                                           gTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTc                                                                                                                                                                                                    <  1:97355/190‑1 (MQ=255)                                                                                                           gTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAAcc                                                                                                                                                                        >  1:247416/1‑218 (MQ=255)                                                                                                                          gCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAgtgt                                                                                                                                                                               >  1:259418/1‑196 (MQ=255)                                                                                                                          gCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCAGCAAAAATCAAAATCCGTGGGTTATTGACTAATGc                                                                                                                                                                                                                                                   <  1:495070/128‑1 (MQ=255)                                                                                                                             aTGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCtt                                                                                                                                                                 <  1:819584/207‑1 (MQ=255)                                                                                                                               gATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTggcg                                                                                                                                                                                                                           <  1:587466/147‑4 (MQ=255)                                                                                                                               gATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGCTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAAc                                                                                                                                                                                    >  1:668244/1‑186 (MQ=255)                                                                                                                                    gCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAAc                                                                                                               >  1:30959/1‑250 (MQ=255)                                                                                                                                       cggTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAAc                                                                                                                                                                                    >  1:21905/3‑178 (MQ=255)                                                                                                                                       cggTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAAc                                                                                                                                                                         >  1:253348/3‑189 (MQ=255)                                                                                                                                         gTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCtttt                                                                                                          >  1:306366/1‑250 (MQ=255)                                                                                                                                         gTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCtttt                                                                                                          >  1:802621/1‑250 (MQ=255)                                                                                                                                         gTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCtttt                                                                                                          >  1:751666/1‑250 (MQ=255)                                                                                                                                                 ttGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTgaca                                                                                                 <  1:468003/251‑4 (MQ=255)                                                                                                                                                         ttttGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCTTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGa                                                                                         >  1:799576/1‑251 (MQ=255)                                                                                                                                                          tttgtgcatattatgcataATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGAt                                                                                        <  1:348178/247‑1 (MQ=255)                                                                                                                                                             gtgcatattatgcataATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTgcgcgcc                                                                                                                                       <  1:839756/200‑4 (MQ=255)                                                                                                                                                                catattatgcataATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGtt                                                                                   <  1:661249/250‑1 (MQ=255)                                                                                                                                                                 atattatgcataATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGAt                                                                                        >  1:569305/1‑244 (MQ=255)                                                                                                                                                                 atattatgcataATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTaa                                                                                 >  1:172610/1‑251 (MQ=255)                                                                                                                                                                  tattatgcataATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTaa                                                                                 >  1:813030/1‑250 (MQ=255)                                                                                                                                                                   attatgcataATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATaa                                                                                                                                                                          <  1:388843/160‑1 (MQ=255)                                                                                                                                                                      atgcataATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTggcg                                                                                                                                                                                                                           <  1:516919/108‑4 (MQ=255)                                                                                                                                                                      atgcataATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAAcc                                                                                                                                                                        >  1:440835/1‑159 (MQ=255)                                                                                                                                                                        gcataATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAAcc                                                                                                                                                                        >  1:14150/1‑157 (MQ=255)                                                                                                                                                                           taATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATtc                                                                       <  1:833537/251‑1 (MQ=255)                                                                                                                                                                                   ttttCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGAt                                                               <  1:705208/251‑1 (MQ=255)                                                                                                                                                                                    tttCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATa                                                              <  1:885928/251‑1 (MQ=255)                                                                                                                                                                                           ttCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAAc                                                                                                                                                                                    <  1:13101/126‑1 (MQ=255)                                                                                                                                                                                            tCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGAtt                                                      <  1:563836/251‑1 (MQ=255)                                                                                                                                                                                              gTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAAcc                                                                                                              <  1:81868/193‑1 (MQ=255)                                                                                                                                                                                              gTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTctctct                                                                  <  1:661709/237‑1 (MQ=255)                                                                                                                                                                                                   cAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAAc                                                                                                                                                                         <  1:330259/129‑1 (MQ=255)                                                                                                                                                                                                     aGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAaca                                              >  1:360603/1‑250 (MQ=255)                                                                                                                                                                                                      gTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCgg                                                                                          >  1:72665/1‑205 (MQ=255)                                                                                                                                                                                                        gCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTgcg                                                                                                                                           >  1:465836/1‑154 (MQ=255)                                                                                                                                                                                                        gCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACAtt                                          >  1:677833/1‑251 (MQ=255)                                                                                                                                                                                                             ggTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTg                                                                                                                                                                                                                        >  1:864862/1‑72 (MQ=255)                                                                                                                                                                                                             ggTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTg                                                                                      <  1:768141/202‑1 (MQ=255)                                                                                                                                                                                                                                            ggTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCgg                                                                                          <  1:684436/169‑1 (MQ=255)                                                                                                                                                                                                                                            ggTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGgcgtaa         >  1:496902/1‑247 (MQ=255)                                                                                                                                                                                                                                                aTTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATtc                                                                       <  1:790001/184‑1 (MQ=255)                                                                                                                                                                                                                                                   gACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATtc                                                                       <  1:11855/181‑1 (MQ=255)                                                                                                                                                                                                                                                   gACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGttt  >  1:592006/1‑250 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     TAAATAAGAGTAAAGACCTTTTTCATCTTTCAGTAATTCCTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATAATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACAGTACGATTTTGGCAAATTTTATGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCTTGGGTTATTGACTAGTGCCCTTGCTATCGCGATCCGTTGACGTTGTCCTCCGGACAAACCGGCTCCCTGTTCCCCCACAACCGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCTCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGGCATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAAGACGACACCGACTTGCCGGCGCAACCAGTTT  >  1/1818853‑1819342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑