Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,819,083	T→G	intergenic (+37183/+16301)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,819,083	0	T	G	100.0%	212.1 / NA	74	intergenic (+37183/+16301)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (33/41);  total (33/41)

TAATTGGTGTAAATAAGAGTAAAGACCTTTTTCATCTTTCAGTAATTCCTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATAATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACAGTACGATTTTGGCAAATTTTATGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCTTGGGTTATTGACTAGTGCCCTTGCTATCGCGATCCGTTGACGTTGTCCTCCGGACAAACCGGCTCCCTGTTCCCCCACAACCGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCTCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGGCATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATT  >  1/1818844‑1819302                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                            tAATTGGTGTAAATAATAGTAAAGGCCTTTTTCATCTTTCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTg                                                                                                                                                                                                                   <  1:522639/250‑1 (MQ=255) tAATTGGTGTAAATAATAGTAAAGGCCTTTTTCATCTTTCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGa                                                                                                                                                                                                                  >  1:392369/1‑251 (MQ=255)  aaTTGGTGTAAATAATAGTAAAGGCCTTTTTCATCTTTCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGAc                                                                                                                                                                                                                 <  1:165265/251‑1 (MQ=255)          tAAATAATAGTAAAGGCCTTTTTCATCTTTCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGccc                                                                                                                                                                                                         <  1:562634/251‑1 (MQ=255)           aaaTAATAGTAAAGGCCTTTTTCATCTTTCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGAc                                                                                                                                                                                                                 <  1:147913/242‑1 (MQ=255)              taatagTAAAGGCCTTTTTCATCTTTCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTg                                                                                                                                                                                                      >  1:758391/5‑250 (MQ=255)               aatagTAAAGGCCTTTTTCATCTTTCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCTTCTCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTg                                                                                                                                                                                                      <  1:681537/248‑1 (MQ=255)                  agTAAAGGCCTTTTTCATCTTTCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGGGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTAt                                                                                                                                                                                                  >  1:187141/1‑250 (MQ=255)                         gccTTTTTCATCTTTCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATc                                                                                                                                                                                           <  1:120910/249‑1 (MQ=255)                                  aTCTTTCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGtt                                                                                                                                                                                 >  1:51108/1‑251 (MQ=255)                                     tttCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTg                                                                                                                                                                                                                   <  1:129918/214‑1 (MQ=255)                                             aTTCTTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGAc                                                                                                                                                                      <  1:160093/251‑1 (MQ=255)                                             aTTCTTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGAc                                                                                                                                                                      <  1:45077/251‑1 (MQ=255)                                              ttcttGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGccc                                                                                                                                                                                                         >  1:873262/5‑215 (MQ=255)                                              ttcttGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGAc                                                                                                                                                                      <  1:115218/246‑1 (MQ=255)                                                     tGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGAc                                                                                                                                                                                                                 >  1:25640/1‑200 (MQ=255)                                                     tGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCgg                                                                                                                                                               >  1:449218/1‑250 (MQ=255)                                                            cTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGt                                                                                                                                                       <  1:103443/251‑1 (MQ=255)                                                              tGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTc                                                                                                                                                     <  1:245272/251‑1 (MQ=255)                                                                      gatTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACaa                                                                                                                                             >  1:275982/1‑251 (MQ=255)                                                                        tttCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAAca                                                                                                                                           >  1:412044/1‑250 (MQ=255)                                                                         ttCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAAcag                                                                                                                                          <  1:484118/251‑3 (MQ=255)                                                                            aCCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAgtgt                                                                                                                                       <  1:590432/251‑1 (MQ=255)                                                                              cTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTta                                                                                                                                     <  1:300699/251‑1 (MQ=255)                                                                               tttGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTtat                                                                                                                                    <  1:14757/251‑1 (MQ=255)                                                                                   tCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAAcc                                                                                                                                <  1:810527/251‑1 (MQ=255)                                                                                   tCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAAc                                                                                                                                 <  1:189648/250‑1 (MQ=255)                                                                                         acaATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAAc                                                                                                                                            <  1:285579/233‑1 (MQ=255)                                                                                         acaATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAAcc                                                                                                                                <  1:98481/245‑1 (MQ=255)                                                                                            atgatGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAAc                                                                                                                                 >  1:43073/4‑241 (MQ=255)                                                                                            atgatGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTaa                                                                                                                       >  1:444671/4‑251 (MQ=255)                                                                                             tgatGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAAt                                                                                                                      <  1:179954/249‑1 (MQ=255)                                                                                                  cGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAg                                                                                                                  <  1:727956/250‑1 (MQ=255)                                                                                                          aTTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAAt                                                                                                                      >  1:34838/1‑238 (MQ=255)                                                                                                                  cAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTgcgcg                                                                                                 >  1:122206/1‑250 (MQ=255)                                                                                                                    gTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTc                                                                                                                                                            <  1:97355/190‑1 (MQ=255)                                                                                                                    gTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAAcc                                                                                                                                >  1:247416/1‑218 (MQ=255)                                                                                                                                   gCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAgtgt                                                                                                                                       >  1:259418/1‑196 (MQ=255)                                                                                                                                   gCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCAGCAAAAATCAAAATCCGTGGGTTATTGACTAATGc                                                                                                                                                                                                           <  1:495070/128‑1 (MQ=255)                                                                                                                                      aTGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCtt                                                                                                                         <  1:819584/207‑1 (MQ=255)                                                                                                                                        gATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTggcg                                                                                                                                                                                   <  1:587466/147‑4 (MQ=255)                                                                                                                                        gATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGCTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAAc                                                                                                                                            >  1:668244/1‑186 (MQ=255)                                                                                                                                             gCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAAc                                                                       >  1:30959/1‑250 (MQ=255)                                                                                                                                                cggTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAAc                                                                                                                                            >  1:21905/3‑178 (MQ=255)                                                                                                                                                cggTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAAc                                                                                                                                 >  1:253348/3‑189 (MQ=255)                                                                                                                                                  gTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCtttt                                                                  >  1:306366/1‑250 (MQ=255)                                                                                                                                                  gTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCtttt                                                                  >  1:802621/1‑250 (MQ=255)                                                                                                                                                  gTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCtttt                                                                  >  1:751666/1‑250 (MQ=255)                                                                                                                                                          ttGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTgaca                                                         <  1:468003/251‑4 (MQ=255)                                                                                                                                                                  ttttGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCTTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGa                                                 >  1:799576/1‑251 (MQ=255)                                                                                                                                                                   tttgtgcatattatgcataATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGAt                                                <  1:348178/247‑1 (MQ=255)                                                                                                                                                                      gtgcatattatgcataATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTgcgcgcc                                                                                               <  1:839756/200‑4 (MQ=255)                                                                                                                                                                         catattatgcataATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGtt                                           <  1:661249/250‑1 (MQ=255)                                                                                                                                                                          atattatgcataATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGAt                                                >  1:569305/1‑244 (MQ=255)                                                                                                                                                                          atattatgcataATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTaa                                         >  1:172610/1‑251 (MQ=255)                                                                                                                                                                           tattatgcataATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTaa                                         >  1:813030/1‑250 (MQ=255)                                                                                                                                                                            attatgcataATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATaa                                                                                                                                  <  1:388843/160‑1 (MQ=255)                                                                                                                                                                               atgcataATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTggcg                                                                                                                                                                                   <  1:516919/108‑4 (MQ=255)                                                                                                                                                                               atgcataATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAAcc                                                                                                                                >  1:440835/1‑159 (MQ=255)                                                                                                                                                                                 gcataATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAAcc                                                                                                                                >  1:14150/1‑157 (MQ=255)                                                                                                                                                                                    taATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATtc                               <  1:833537/251‑1 (MQ=255)                                                                                                                                                                                            ttttCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGAt                       <  1:705208/251‑1 (MQ=255)                                                                                                                                                                                             tttCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATa                      <  1:885928/251‑1 (MQ=255)                                                                                                                                                                                                    ttCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAAc                                                                                                                                            <  1:13101/126‑1 (MQ=255)                                                                                                                                                                                                     tCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGAtt              <  1:563836/251‑1 (MQ=255)                                                                                                                                                                                                       gTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAAcc                                                                      <  1:81868/193‑1 (MQ=255)                                                                                                                                                                                                       gTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTctctct                          <  1:661709/237‑1 (MQ=255)                                                                                                                                                                                                            cAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAAc                                                                                                                                 <  1:330259/129‑1 (MQ=255)                                                                                                                                                                                                              aGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAaca      >  1:360603/1‑250 (MQ=255)                                                                                                                                                                                                               gTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCgg                                                  >  1:72665/1‑205 (MQ=255)                                                                                                                                                                                                                 gCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTgcg                                                                                                   >  1:465836/1‑154 (MQ=255)                                                                                                                                                                                                                 gCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACAtt  >  1:677833/1‑251 (MQ=255)                                                                                                                                                                                                                      ggTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTg                                                                                                                                                                                >  1:864862/1‑72 (MQ=255)                                                                                                                                                                                                                      ggTTGCT‑‑TCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTg                                              <  1:768141/202‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                            TAATTGGTGTAAATAAGAGTAAAGACCTTTTTCATCTTTCAGTAATTCCTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATAATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACAGTACGATTTTGGCAAATTTTATGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCT‑‑TCATCAAAAATCAAAATCCTTGGGTTATTGACTAGTGCCCTTGCTATCGCGATCCGTTGACGTTGTCCTCCGGACAAACCGGCTCCCTGTTCCCCCACAACCGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCTCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGGCATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATT  >  1/1818844‑1819302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑