Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,818,828	T→C	intergenic (+36928/+16556)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,818,828	0	T	C	100.0%	142.4 / NA	52	intergenic (+36928/+16556)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/27);  total (25/27)

TTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGAATAAGACACGAATCGTGGTGCATTTGATACAGGTGTTTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAACCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTTCCTTTAATTTACTTGTAATTGGTGTAAATAAGAGTAAAGACCTTTTTCATCTTTCAGTAATTCCTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATAATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACAGTACGATTTTGGCAAATTTTATGCATATTATGCATAATGATATTTTCAGATTCGTAATCA  >  1/1818582‑1819048                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                             ttGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGAATAAGACACGAATCGTGGTGCATTTGATACAGGTGTCTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCtt                                                                                                                                                                                                                          >  1:170514/1‑251 (MQ=255)   gCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGAATAAGACACGAATCGTGGTGCATTTGATACAGGTGTCTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTa                                                                                                                                                                                                                        >  1:203039/1‑251 (MQ=255)   gCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGAATAAGACACGAATCGTGGTGCATTTGATACAGGTGTCTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTa                                                                                                                                                                                                                        >  1:332690/1‑251 (MQ=255)       aaGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGAATAAGACACGAATCGTGGTGCATTTGATACAGGTGTCTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAAttt                                                                                                                                                                                                                    <  1:376707/251‑1 (MQ=255)                          cTAAAGTTAAAAAGAGCATAATGGAATAAGACACGAATCGTGGTGCATTTGATACAGGTGTCTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGGTGTAAAt                                                                                                                                                                                                 <  1:857617/251‑1 (MQ=255)                               gTTAAAAAGAGCATAATGGAATAAGACACGAATCGTGGTGCATTTGATACAGGTGTCTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGGTGTAAATaatag                                                                                                                                                                                            >  1:541107/1‑248 (MQ=255)                                ttAAAAAGAGCATAATGGAATAAGACACGAATCGTGGTGCATTTGATACAGGTGTCTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGGTGTAAATaatag                                                                                                                                                                                            <  1:840787/250‑4 (MQ=255)                                        agCATAATGGAATAAGACACGAATCGTGGTGCATTTGATACAGGTGTCTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGGTGTAAATAATAGTAAAggcc                                                                                                                                                                                    <  1:174502/250‑4 (MQ=255)                                        agCATAATGGAATAAGACACGAATCGTGGTGCATTTGATACAGGTGTCTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGGTGTAAATAATAGTAAAggc                                                                                                                                                                                     <  1:243533/249‑3 (MQ=255)                                        agCATAATGGAATAAGACACGAATCGTGGTGCATTTGATACAGGTGTCTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGGTGTAAATAATAGTAAAggc                                                                                                                                                                                     <  1:322817/249‑3 (MQ=255)                                         gCATAATGGAATAAGACACGAATCGTGGTGCATTTGATACAGGTGTCTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTAc                                                                                                                                                                                                                  >  1:354216/1‑219 (MQ=255)                                            tAATGGAATAAGACACGAATCGTGGTGCATTTGATACAGGTGTCTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCCCACGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGgtg                                                                                                                                                                                                      <  1:401110/228‑1 (MQ=255)                                            tAATGGAATAAGACACGAATCGTGGTGCATTTGATACAGGTGTCTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGGTGTAAATa                                                                                                                                                                                                <  1:653149/234‑1 (MQ=255)                                                 gAATAAGACACGAATCGTGGTGCATTTGATACAGGTGTCTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGGTGTAAATAATAGTAAAGGCCTTTTTCAt                                                                                                                                                                            >  1:266693/1‑249 (MQ=255)                                                      aGACACGAATCGTGGTGCATTTGATACAGGTGTCTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTaa                                                                                                                                                                                                                       >  1:261361/1‑201 (MQ=255)                                                      aGACACGAATCGTGGTGCATTTGATACAGGTGTCTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAAttt                                                                                                                                                                                                                    >  1:247505/1‑204 (MQ=255)                                                       gACACGAATCGTGGTGCATTTGATACAGGTGTCTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGGTGTAAATAATAGTAAAGGCCTTTTTCATCTTTCAGt                                                                                                                                                                    >  1:839389/1‑251 (MQ=255)                                                         cacGAATCGTGGTGCATTTGATACAGGTGTCTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGGTGTAAATa                                                                                                                                                                                                >  1:180281/1‑221 (MQ=255)                                                                     tgCATTTGATACAGGTGTCTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGGTGTAAATAATAGTAAAGGCCTTTTTCATCTTTCAGTAATTCTTGATGCt                                                                                                                                                       <  1:187489/250‑1 (MQ=255)                                                                          gctatggcaaaaccaccttaatttCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGGTGTAAATAATAGTAAAGGCCTTTTTCATCTTTCAGTAATTCTTGATGCTTGc                                                                                                                                                    <  1:637580/226‑1 (MQ=255)                                                                           tGATACAGGTGTCTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGGTGTAAATaatag                                                                                                                                                                                            >  1:612330/1‑204 (MQ=255)                                                                             aTACAGGTGTCTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGGTGTAAATAATAGTAAAGGCCTTTTTCATCTTTCAGTAATTCTTGATGCTTGCCTTGtt                                                                                                                                              >  1:221448/1‑251 (MQ=255)                                                                                      tctCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGgtg                                                                                                                                                                                                      <  1:597436/184‑1 (MQ=255)                                                                                         cAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGGTGTAAATaatag                                                                                                                                                                                            <  1:382104/193‑4 (MQ=255)                                                                                         cAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGGTGTAAATAATAGTAAAGGCCTTTTTCATCTTTCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCa                                                                                                                                   >  1:550636/1‑250 (MQ=255)                                                                                         cAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGGTGTAAATAATAGTAAAGGCCTTTTTCATCTTTCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCa                                                                                                                                   >  1:111049/1‑250 (MQ=255)                                                                                          aattaattCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGGTGTAAATAATAGTAAAGGCCTTTTTCATCTTTCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCAcc                                                                                                                                 <  1:844384/251‑1 (MQ=255)                                                                                           attaattCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGGTGTAAATAATAGTAAAGGCCTTTTTCATCTTTCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCAc                                                                                                                                  >  1:768445/1‑249 (MQ=255)                                                                                            ttaattCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGGTGTAAATAATAGTAAAGGCCTTTTTCATCTTTCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCACCtt                                                                                                                               <  1:361004/251‑1 (MQ=255)                                                                                                               cAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGGTGTAAATAATAGTAAAGGCCTTTTTCATCTTTCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACaatga                                                                                                                <  1:540651/247‑3 (MQ=255)                                                                                                               cAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGGTGTAAATAATAGTAAAGGCCTTTTTCATCTTTCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGc                                                                                                             <  1:470762/250‑1 (MQ=255)                                                                                                                 gAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAAtt                                                                                                                                                                                                                     >  1:92343/1‑144 (MQ=255)                                                                                                                            tCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGGTGTAAATAATAGTAAAGGCCTTTTTCATCTTTCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCAtttttt                                                                                               <  1:156740/251‑1 (MQ=255)                                                                                                                                cTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGGTGTAAATAATAGTAAAGGCCTTTTTCATCTTTCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAg                                                                                                       >  1:426634/1‑239 (MQ=255)                                                                                                                                         taatcGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGGTGTAAATAATAGTAAAGGCCTTTTTCATCTTTCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCAcc                                                                                                                                 <  1:870405/200‑1 (MQ=255)                                                                                                                                                  aaTCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGGTGTAAATAATAGTAAAGGCCTTTTTCATCTTTCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGc                                                                           <  1:209156/249‑1 (MQ=255)                                                                                                                                                           gTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGGTGTAAATAATAGTAAAGGCCTTTTTCATCTTTCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAgg                                                                                   >  1:303518/1‑232 (MQ=255)                                                                                                                                                            tttACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGGTGTAAATAATAGTAAAGGCCTTTTTCATCTTTCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGcac                                                               <  1:629863/251‑1 (MQ=255)                                                                                                                                                                                 aTTCCACACGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGGTGTAAATAATAGTAAAGGCCTTTTACATCTTTCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAatgatg                                                                                                              <  1:570660/183‑1 (MQ=255)                                                                                                                                                                                         cGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGGTGTAAATAATAGTAAAgg                                                                                                                                                                                      >  1:306218/1‑102 (MQ=255)                                                                                                                                                                                         cGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGGTGTAAATAATAGTAAAGGCCTTTTTCATCTTTCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCGGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTttgtgcatat                                  >  1:726477/1‑251 (MQ=255)                                                                                                                                                                                         cGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGGTGTAAATAATAGTAAAGGCCTTTTTCATCTTTCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTttgtgcatat                                  >  1:874640/1‑251 (MQ=255)                                                                                                                                                                                           tttttATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGGTGTAAATAATAGTAAAGGCCTTTTTCATCTTTCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTttgtgcatatt                                 <  1:54323/250‑1 (MQ=255)                                                                                                                                                                                           tttttATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTAGTCCCTTTAATTTACTTGTAATTGGTGTAAATAATAGTAAAGGCCTTTTTCATCTTTCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTttgtgcatatta                                >  1:406267/1‑251 (MQ=255)                                                                                                                                                                                                              catcatACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGGTGTAAATAATAGTAAAGGCCTTTTTCATCTTTCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATa                                                                    <  1:666290/196‑1 (MQ=255)                                                                                                                                                                                                              catcatACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGGTGTAAATAATAGTAAAGGCCTTTTTCATCTTTCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAg             <  1:164781/251‑1 (MQ=255)                                                                                                                                                                                                                 catACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGGTGTAAATAATAGTAAAGGCCTTTTTCATCTTTCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGAtt          <  1:829776/251‑1 (MQ=255)                                                                                                                                                                                                                  atACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGGTGTAAATAATAGTAAAGGCCTTTTTCATCTTTCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAgg                                                                                   >  1:699597/1‑177 (MQ=255)                                                                                                                                                                                                                  atACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGGTGTAAATAATAGTAAAGGCCTTTTTCATCTTTCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAgg                                                                                   >  1:9122/1‑177 (MQ=255)                                                                                                                                                                                                                  atACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGGTGTAAATAATAGTAAAGGCCTTTTTCATCTTTCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTttgtgcatattatgca                            <  1:706649/232‑1 (MQ=255)                                                                                                                                                                                                                   tACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGGTGTAAATAATAGTAAAGGCCTTTTTCATCTTTCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTttgtgcatattatgc                             >  1:779986/1‑230 (MQ=255)                                                                                                                                                                                                                          ccAAAAGCCAAGTTTTCATTATCGTTCGTCCCTTTAATTTACTTGTAATTGGTGTAAATAATAGTAAAGGCCTTTTTCATCTTTCAGTAATTCTTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATGATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCa  <  1:584052/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                             TTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGAATAAGACACGAATCGTGGTGCATTTGATACAGGTGTTTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAACCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACAATGCCAAAAGCCAAGTTTTCATTATCGTTCGTTCCTTTAATTTACTTGTAATTGGTGTAAATAAGAGTAAAGACCTTTTTCATCTTTCAGTAATTCCTGATGCTTGCCTTGTTCGATGATTTCACCTTTGTCCATCACAATAATGCGATCAGCATTTTTTACAGTAGAAAGGCGGTGAGCAATGATAAGCACAGTACGATTTTGGCAAATTTTATGCATATTATGCATAATGATATTTTCAGATTCGTAATCA  >  1/1818582‑1819048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑