Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,817,293	C→T	intergenic (+35393/+18091)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,817,293	0	C	T	100.0%	43.6 / NA	17	intergenic (+35393/+18091)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/12);  total (5/12)

CACTTGAATTTTTGACCGCACTTTTGC‑CCCGAAAACCTACAACGGGCTTTCCACTTGCAGGCGTTCGCAAATGCCGGTAATGCGCTTTCAATTATAAATCCATCATCAAGTCACAGTTTTATACTGCCTAACCAAATCTATTTTCAATTTAGACTTTTGATACATTGTGGAAAACCGGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTAAGTAGATAGCTTATAACGCTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCGGAAAGTGGGATTTCTTTTTCTTCGGTAGAAAGTG  >  1/1817182‑1817526                                                                                                                 |                                                                                                                                                                                                                                          caccggggaaatccaCCGCACTTTTGCACCAGAAAA‑CTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGt                                                                                                <  1:429082/237‑1 (MQ=255)      gggaaatccaCCGCACTTTTGCACCAGAAAA‑CTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGAt                                                                                                                                                                                          >  1:572457/10‑156 (MQ=255)      gggaaatccaCCGCACTTTTGCACCAGAAAA‑CTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTtat                                                                                                                                                   >  1:699651/10‑195 (MQ=255)        gaaatccaCCGCACTTTTGCACCAGAAAA‑CTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAg                                                                                          >  1:884751/8‑250 (MQ=255)         aaatccaCCGCACTTTTGCACCAGAAAA‑CTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGc                                                                                         <  1:207804/244‑1 (MQ=255)               aCCGCACTTTTGCACCAGAAAA‑CTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAAc                                                                                  <  1:443109/251‑1 (MQ=255)               aCCGCACTTTTGCACCAGAAAA‑CTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAAc                                                                                  <  1:296055/251‑1 (MQ=255)                     cTTTTGCACCAGAAAA‑CTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTc                                                                                                                                                             <  1:845446/170‑1 (MQ=255)                           caccagAAAA‑CTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGcc                                                                      <  1:873801/246‑1 (MQ=255)                                      cTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAAtt                                                            <  1:295554/251‑1 (MQ=255)                                      cTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAAtt                                                            <  1:214057/251‑1 (MQ=255)                                        aCAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAAttt                                                           >  1:881110/1‑250 (MQ=255)                                                     ggcttgctggtattCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAAc                                                                                  <  1:751097/202‑1 (MQ=255)                                                     ggcttgctggtattCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGcc                                                                      >  1:338020/13‑226 (MQ=255)                                                                                            aTTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTatat                                                                                                                                                               <  1:663152/98‑1 (MQ=255)                                                                                                taAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAgtg  <  1:142307/251‑1 (MQ=255)                                                                                                         catcaAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGccc                                                                     <  1:157105/175‑2 (MQ=255)                                                                                                                 |                                                                                                                                                                                                                                          CACTTGAATTTTTGACCGCACTTTTGC‑CCCGAAAACCTACAACGGGCTTTCCACTTGCAGGCGTTCGCAAATGCCGGTAATGCGCTTTCAATTATAAATCCATCATCAAGTCACAGTTTTATACTGCCTAACCAAATCTATTTTCAATTTAGACTTTTGATACATTGTGGAAAACCGGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTAAGTAGATAGCTTATAACGCTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCGGAAAGTGGGATTTCTTTTTCTTCGGTAGAAAGTG  >  1/1817182‑1817526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑