Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,817,129	C→A	intergenic (+35229/+18255)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,817,129	0	C	A	100.0%	134.3 / NA	47	intergenic (+35229/+18255)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (23/24);  total (23/24)

CGTGTATTCCTGTGAAGCGGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTAGAACGCTATATGCAATCTTTCGCCGATGAAATGGCGTGCTTTGTGGATTCCGTAGTAAACGACAAACCGACTTTAGTCAACGGCAACGACGGCTTGCAACCTGTGCTGATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCT‑AAAGCATTTTCTGATGCT‑AAAAAAGACCTGAAACCGATTCAGGTCTTTTGTTTTTCGG‑TGATTAGAAAACACTTGAATTTTTGACCGCACTTTTGCCCCGAAAACCTACAACGGGCTTTCCACTTGCAGGCGTTCGCAAATGCCGGTAATGCGCTTTCAATTATAAATCCATCATC  >  1/1816886‑1817288                                                                                                                                                                                                                                                     |                                                                                                                                                                  cgTGTATTCCTGTGAAGCAGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGAT‑aaaaaa                                                                                                                                                           <  1:396822/251‑1 (MQ=255)   tgtATTCCTGTGAAGCAGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTCATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGAT‑aaaaaaag                                                                                                                                                         <  1:49216/251‑3 (MQ=255)     tATTCCTGTGAAGCAGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGAT‑aaaaaaagac                                                                                                                                                       <  1:349131/251‑5 (MQ=255)           tgtgAAGCAGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGaag                                                                                                                                                  <  1:135529/251‑2 (MQ=255)           tgtgAAGCAGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGaag                                                                                                                                                  <  1:393287/251‑2 (MQ=255)                               cGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGttt                                                                                                                              <  1:858422/251‑1 (MQ=255)                                            tatTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCggcgggttaa                                                                                                                 >  1:539021/1‑243 (MQ=255)                                                   tcCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCggcgggttaaaaaaca                                                                                                           >  1:485296/1‑236 (MQ=255)                                                            gCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGaagcgaattc                                                                                                                                           >  1:814593/1‑201 (MQ=255)                                                            gCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAAtttt                                                                                                 >  1:496082/1‑251 (MQ=255)                                                            gCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAAtttt                                                                                                 >  1:418922/1‑251 (MQ=255)                                                               atatGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAACA‑TTTCCGATGATAAAAAAAGACCTGa                                                                                                                                                    >  1:452156/1‑197 (MQ=255)                                                                                        ggCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGCCCCG‑AAACCTACa                                                                    >  1:894462/1‑251 (MQ=255)                                                                                             gCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGCCCCG‑AAACCTACAACggg                                                               >  1:245466/1‑251 (MQ=255)                                                                                              cTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGCCCCG‑AAACCTACAACggg                                                               <  1:803288/250‑1 (MQ=255)                                                                                              cTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGCCCCG‑AAACCTACAACGGGc                                                              <  1:296894/251‑1 (MQ=255)                                                                                                ttGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGCCCCG‑AAACCTACAACggg                                                               <  1:707937/248‑1 (MQ=255)                                                                                                  gtgGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGCCCCG‑AAACCTACAACGGGCttt                                                           >  1:230784/1‑250 (MQ=255)                                                                                                    ggATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCggcgggttaaaaaac                                                                                                            <  1:435423/200‑14 (MQ=255)                                                                                                     gATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGCCCCG‑AAACCTACAACGGGCTTTcgg                                                        <  1:568767/250‑3 (MQ=255)                                                                                                      aTTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAAt                                                                                                    >  1:785189/1‑206 (MQ=255)                                                                                                      aTTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGCCCCG‑AAACCTACAACGGGCTTTcggtt                                                      >  1:679263/1‑247 (MQ=255)                                                                                                      aTTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGCCCCG‑AAACCTACAACGGGCTTTcggtt                                                      >  1:27254/1‑247 (MQ=255)                                                                                                         ccGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGCCCCG‑AAACCTACAACGGGCTTTcggtttg                                                    >  1:312966/1‑244 (MQ=255)                                                                                                           gtagtgAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGCCCCG‑AAACCTACAACGGGCTTTcggtttgcag                                                 >  1:28084/1‑242 (MQ=255)                                                                                                            tagtgAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGCCCCG‑AAACCTACAACGGGCTTTcggtttgcagg                                                <  1:816868/251‑11 (MQ=255)                                                                                                            tagtgAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGCCCCG‑AAACCTACAACGGGCTTTcggtttgcagg                                                <  1:569377/251‑11 (MQ=255)                                                                                                               tgaaCGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGCCCCG‑AAACCTACAACGGGCTTTCGGTTTGCAGGCg                                              <  1:384749/248‑1 (MQ=255)                                                                                                                             aCTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGCCCCG‑AAACCTACAACGGGCTTTCGGTTTGCAGGCGTTCGCAAATGTCGGt                               >  1:881958/1‑251 (MQ=255)                                                                                                                               tttAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGCCCCG‑AAACCTACAACGGGCTTTCGGTTTGCAGGCGTTCGCAAATGTCGGTaa                             <  1:325185/251‑1 (MQ=255)                                                                                                                                   gTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGCCCCG‑AAACCTACAACGGGCTTTCGGTTTGCAGGCGTTCGCAAATGTCGGTAATggg                         >  1:612072/1‑249 (MQ=255)                                                                                                                                    tCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGCCCCG‑AAACCTACAACGGGCTTTCGGTTTGCAGGCGTTCGCAAATGTCGGTAATggtg                        <  1:861517/251‑4 (MQ=255)                                                                                                                                    tCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGCCCCG‑AAACCTACAACGGGCTTTCGGTTTGCAGGCGTTCGCAAATGTCGGTAATggtg                        <  1:891276/251‑4 (MQ=255)                                                                                                                                    tCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGCCCCG‑AAACCTACAACGGGCTTTCGGTTTGCAGGCGTTCGCAAATGTCGGTAATgg                          <  1:778134/249‑2 (MQ=255)                                                                                                                                       aCGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGCCCCG‑AAACCTACAACGGGCTTTCGGTTTGCAGGCGTTCGCAAATGTCGGTAATggtgaga                     <  1:603299/251‑7 (MQ=255)                                                                                                                                        cGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGCCCCG‑AAACCTACAACGGGCTTTCGGTTTGCAGGCGTTCGCAAATGTCGGTAATggtgagaa                    <  1:620902/251‑8 (MQ=255)                                                                                                                                        cGGCAACGACGGTTTGCAACATGTGCTTATCGCGTTGGCGAGAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCAGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACTCTTGAATTTTTGACCGCACTTTTGCCCCG‑AAACCTACAACGGGCTTTCGGTTTGCAGGCGTTCGCAAATGTCGGTAATggtgagaa                    <  1:452723/251‑8 (MQ=255)                                                                                                                                         ggCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGCCCCG‑AAACCTACAACGGGCTTTCGGTTTGCAGGCGTTCGCAAATGTCGGTAATggtgagaat                   >  1:165049/1‑243 (MQ=255)                                                                                                                                                   gtttGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGCCCCG‑AAACCTACAACGGGCTTTcggtttgca                                                  >  1:56981/3‑202 (MQ=255)                                                                                                                                                   gtttGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGCCCCG‑AAACCTACAACGGGCTTTCGGTTTGCAGGCGTTCGCAAATGTCGGTAATggtgagaatgaggctggag         >  1:52282/3‑233 (MQ=255)                                                                                                                                                   gtttGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGCCCCG‑AAACCCACAACGGGCTTTCGGTTTGCAGGCGTTCGCAAATGTCGGTAATggtgagaatgaggctggag         >  1:55082/3‑233 (MQ=255)                                                                                                                                                    tttGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGCCCCG‑AAACCTACAACGGGCTTTCGGTTTGCAGGCGTTCGCAAATGTCGGTAATggtgagaatgaggctggag         <  1:375722/249‑19 (MQ=255)                                                                                                                                                           ccTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGCCCCG‑AAACCTACAACGGGCTTTCGGTTTGCAGGCGTTCGCAAATGTCGGTAATggtgagaatgaggctggagcggataa  >  1:799382/1‑225 (MQ=255)                                                                                                                                                           ccTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGCCCCG‑AAACCTACAACGGGCTTTCGGTTTGCAGGCGTTCGCAAATGTCGGTAATggtgagaatgaggctggagcggataa  >  1:332391/1‑225 (MQ=255)                                                                                                                                                                               ggcAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGCCCCG‑AAACCTAc                                                                     <  1:615349/163‑1 (MQ=255)                                                                                                                                                                                     aCGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGCCCCG‑AAACCTACAACGGGCTTTCGGTTTGCAGGCGTTCGCAAATg                                    >  1:258650/1‑190 (MQ=255)                                                                                                                                                                                               gatgaAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAATTTTTGACCGCACTTTTGCCCCG‑AAACCTACAACGGGCTTTCGGTTTGCAGGCGTTCGCAAATGTCGGTAAt                            <  1:260690/188‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                  CGTGTATTCCTGTGAAGCGGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTAGAACGCTATATGCAATCTTTCGCCGATGAAATGGCGTGCTTTGTGGATTCCGTAGTAAACGACAAACCGACTTTAGTCAACGGCAACGACGGCTTGCAACCTGTGCTGATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCT‑AAAGCATTTTCTGATGCT‑AAAAAAGACCTGAAACCGATTCAGGTCTTTTGTTTTTCGG‑TGATTAGAAAACACTTGAATTTTTGACCGCACTTTTGCCCCGAAAACCTACAACGGGCTTTCCACTTGCAGGCGTTCGCAAATGCCGGTAATGCGCTTTCAATTATAAATCCATCATC  >  1/1816886‑1817288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑