Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,816,685	C→T	intergenic (+34785/+18699)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,816,685	0	C	T	100.0%	206.8 / NA	70	intergenic (+34785/+18699)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (38/32);  total (38/32)

GAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGTGAACCGCACTTAATCCGCGTTACCTCACGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTATCCAGCGGGATGTTCTTTGACATGACCATTCACGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTGGTAGAAGTGTACGCGGCGGGCGGCGTGCTGGTGAATCCTGAAATCGGCAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCAATCGGTGTGATCGACAACAGCCGCAAAGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGCAATGATACCGATTCCACCGCCGTGTATTCCTGTGAAGCGGGCGTGATTGCCGAAAAACCGAAATAT  >  1/1816440‑1816931                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       gAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGAGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATgcgg                                                                                                                                                                                                                                                    >  1:431605/1‑250 (MQ=255)                aaaTTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTgg                                                                                                                                                                                                                                     <  1:553543/250‑1 (MQ=255)                                                  acaACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGac                                                                                                                                                                                                  <  1:826627/251‑1 (MQ=255)                                                   caACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGaca                                                                                                                                                                                                 >  1:342681/1‑251 (MQ=255)                                                     aCTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGacac                                                                                                                                                                                                >  1:882184/1‑250 (MQ=255)                                                     aCTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGacac                                                                                                                                                                                                >  1:328400/1‑250 (MQ=255)                                                      cTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATAtt                                                                                                                                                                                                     >  1:233628/1‑244 (MQ=255)                                                      cTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACAccg                                                                                                                                                                                              >  1:836599/1‑251 (MQ=255)                                                        ttAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACAccgcc                                                                                                                                                                                            <  1:247862/251‑1 (MQ=255)                                                               cATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGtcatca                                                                                                                                                                                     >  1:223551/1‑251 (MQ=255)                                                                aTCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGaca                                                                                                                                                                                                 <  1:566258/238‑1 (MQ=255)                                                                    aGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTa                                                                                                                                                                                >  1:639976/1‑251 (MQ=255)                                                                     gACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTaa                                                                                                                                                                               >  1:52184/1‑251 (MQ=255)                                                                            gTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTgg                                                                                                                                                                         >  1:169640/1‑250 (MQ=255)                                                                                              ggcgAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGGGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGgtgg                                                                                                                                                      >  1:676035/4‑250 (MQ=255)                                                                                                    ccGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGa                                                                                                                                                >  1:827804/1‑251 (MQ=255)                                                                                                       cACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGac                                                                                                                                                                                                  >  1:671614/1‑198 (MQ=255)                                                                                                       cACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGgtgtaat                                                                                                                                                   <  1:157099/245‑4 (MQ=255)                                                                                                            aaTCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAGTCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGc                                                                                                                                         >  1:451556/1‑250 (MQ=255)                                                                                                                          cccgcgACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTa                                                                                                                           >  1:594154/3‑250 (MQ=255)                                                                                                                                agccTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTa                                                                                                                     >  1:142619/3‑250 (MQ=255)                                                                                                                                       cGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCaa                                                                                                              >  1:851316/1‑250 (MQ=255)                                                                                                                                                 aTTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAg                                                                                                    <  1:564885/250‑1 (MQ=255)                                                                                                                                                 aTTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAg                                                                                                    <  1:115840/250‑1 (MQ=255)                                                                                                                                                    gAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGc                                                                                                                                         <  1:800144/210‑1 (MQ=255)                                                                                                                                                        aCGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGc                                                                                            <  1:803942/251‑1 (MQ=255)                                                                                                                                                         cGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGGCACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCgg                                                                                             >  1:656870/1‑249 (MQ=255)                                                                                                                                                         cGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCgg                                                                                             >  1:211775/1‑249 (MQ=255)                                                                                                                                                         cGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGGGTTGGAGAATCCGGAAATCGGTAAAACCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGGGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGGTCGGc                                                                                            >  1:441463/1‑250 (MQ=255)                                                                                                                                                           ttAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTc                                                                                          <  1:845510/250‑1 (MQ=255)                                                                                                                                                              aaGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGaaa                                                                                      <  1:842736/251‑1 (MQ=255)                                                                                                                                                                gtgtccggCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAgg                                                                                    >  1:204705/8‑251 (MQ=255)                                                                                                                                                                        cGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTAc                                                                            <  1:727847/251‑1 (MQ=255)                                                                                                                                                                         gggATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCg                                                                                                                                                 >  1:606487/1‑181 (MQ=255)                                                                                                                                                                         gggATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACggg                                                                                                          <  1:521151/220‑1 (MQ=255)                                                                                                                                                                             tGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAAcc                                                                       <  1:208491/251‑1 (MQ=255)                                                                                                                                                                             tGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAAcc                                                                       <  1:688104/251‑1 (MQ=255)                                                                                                                                                                             tGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAAcc                                                                       <  1:132471/251‑1 (MQ=255)                                                                                                                                                                                tCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATTCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGtcatc                                                                                                                                                                                      >  1:649999/1‑137 (MQ=255)                                                                                                                                                                                tCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAAc                                                                                                                                                                            >  1:77146/1‑147 (MQ=255)                                                                                                                                                                                tCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCg                                                                                                                                                 <  1:413891/174‑1 (MQ=255)                                                                                                                                                                                tCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGAcc                                                                                                                <  1:125163/207‑1 (MQ=255)                                                                                                                                                                                  tttGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCgtaa                                                                  <  1:395590/251‑4 (MQ=255)                                                                                                                                                                                    tGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATg                                                                <  1:873218/251‑1 (MQ=255)                                                                                                                                                                                    tGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATg                                                                <  1:599622/251‑1 (MQ=255)                                                                                                                                                                                    tGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATg                                                                <  1:445639/251‑1 (MQ=255)                                                                                                                                                                                    tGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTCACACCGCCGTCATCAACTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATg                                                                <  1:270088/251‑1 (MQ=255)                                                                                                                                                                                     gACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATGa                                                               >  1:883658/1‑251 (MQ=255)                                                                                                                                                                                        aTGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATGATAc                                                            <  1:390884/251‑1 (MQ=255)                                                                                                                                                                                             cATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATGATACCGAtt                                                       <  1:405952/251‑1 (MQ=255)                                                                                                                                                                                              aTTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATGATAc                                                            >  1:863885/1‑245 (MQ=255)                                                                                                                                                                                                   tgATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATGATACCGATTCTAccg                                                 >  1:854591/2‑251 (MQ=255)                                                                                                                                                                                                      tttCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCg                                                                                                                                                 <  1:558099/152‑1 (MQ=255)                                                                                                                                                                                                          gACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCgtaa                                                                  <  1:669720/227‑4 (MQ=255)                                                                                                                                                                                                              tGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATg                                                                >  1:727571/1‑225 (MQ=255)                                                                                                                                                                                                              tGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATGATACCGATTCTACCGCCGTGTATTcc                                      <  1:340687/251‑1 (MQ=255)                                                                                                                                                                                                               gATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGGGGCGCGTTGGAGAATCCGGAAATCGGTAAACGCGGCGATATTGacac                                                                                                                                                                                                >  1:840944/1‑96 (MQ=255)                                                                                                                                                                                                                     ttatttatCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATGATACCGATTCTACCGCCGTGTATTCCTGTGAAg                               <  1:80231/251‑1 (MQ=255)                                                                                                                                                                                                                     ttatttatCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATGATACCGATTCTACCGCCGTGTATTCCTGTGAAg                               <  1:148011/251‑1 (MQ=255)                                                                                                                                                                                                                                gCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGGGTAATCGCCAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTTGGCTCGAAAGGCGCGGAACAAACCCGTAATGATACCGATCCTACCGCCGTGTATTCCTGTGAAGCAGGCGGGAtt                    >  1:108003/1‑251 (MQ=255)                                                                                                                                                                                                                                 cAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATGATACCGATTCTACCGCCGTGTATTCCTGTGAAGCAGGCGTGAtt                    <  1:355524/250‑1 (MQ=255)                                                                                                                                                                                                                                      aagtcgtggAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATGATACCGATTCTACCGCCGTGTATTCCTGTGAAGCAGGCGTGATTGCCGaa              >  1:377493/9‑251 (MQ=255)                                                                                                                                                                                                                                       agtcgtggAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAAcc                                                                       >  1:222540/8‑193 (MQ=255)                                                                                                                                                                                                                                        gtcgtggAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATGATACCGATTCTACCGCCGTGTATTCCTGTGAAGCAGGCg                         >  1:77653/7‑238 (MQ=255)                                                                                                                                                                                                                                         tcgtggAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAAc                                                                                                                                                                            <  1:277397/85‑1 (MQ=255)                                                                                                                                                                                                                                              gAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATGATACCGATTCTAccgc                                                >  1:498593/1‑209 (MQ=255)                                                                                                                                                                                                                                              gAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATGATACCGATTCTACCGCCGTGTATTCCTGTGAAGCAGGCGTGATTGCCGAAAAACCGaa      >  1:43220/1‑251 (MQ=255)                                                                                                                                                                                                                                                aGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATGATAc                                                            >  1:546193/1‑195 (MQ=255)                                                                                                                                                                                                                                                aGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAAGCGCGGTACAAACCCGTAATGATACCGATTCTACCGCCGTGTATTCCTGTGAAGCAGGCGTGATCGCCGAAAAACCGaaaa    >  1:451513/1‑250 (MQ=255)                                                                                                                                                                                                                                                  tgtATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATGATACCGATTCTACCGCCGTGTATTCCTGTGAAGCAGGCGTGATTGCCGAAAAACCGAAatat  >  1:350067/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       GAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGTGAACCGCACTTAATCCGCGTTACCTCACGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTATCCAGCGGGATGTTCTTTGACATGACCATTCACGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTGGTAGAAGTGTACGCGGCGGGCGGCGTGCTGGTGAATCCTGAAATCGGCAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCAATCGGTGTGATCGACAACAGCCGCAAAGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGCAATGATACCGATTCCACCGCCGTGTATTCCTGTGAAGCGGGCGTGATTGCCGAAAAACCGAAATAT  >  1/1816440‑1816931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑