Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,816,605	A→G	intergenic (+34705/+18779)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,816,605	0	A	G	100.0%	162.5 / NA	57	intergenic (+34705/+18779)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/24);  total (33/24)

TGCCGGCAAACACATTTTCTGTGAAAAACCGGTGGATGCCGATGTGAATCGCATTAAAGAAGTCTTAGTCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGTGAACCGCACTTAATCCGCGTTACCTCACGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTATCCAGCGGGATGTTCTTTGACATGACCATTCACGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTGGTAGAAGTGTACGCGGCGGGCGGCGTGCTGGTGAATCCTGAAATCGGCAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCAATCGGTGTGATCGACAACAGCCGCAAAGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAA  >  1/1816364‑1816847                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   tGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATg                                                                                                                                                                                                                                            <  1:565819/250‑1 (MQ=255)       cAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCttt                                                                                                                                                                                                                                      <  1:303974/250‑1 (MQ=255)                      cgAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCatg                                                                                                                                                                                                                      <  1:748338/250‑3 (MQ=255)                             ccGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGa                                                                                                                                                                                                               >  1:887966/1‑251 (MQ=255)                             ccGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGa                                                                                                                                                                                                               >  1:516256/1‑251 (MQ=255)                                gTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGAc                                                                                                                                                                                                                                   <  1:42577/228‑1 (MQ=255)                                gTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACa                                                                                                                                                                                                             <  1:674570/250‑1 (MQ=255)                                gTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACa                                                                                                                                                                                                             <  1:374561/250‑1 (MQ=255)                                    aTGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGAt                                                                                                                                                                                                         >  1:678989/1‑250 (MQ=255)                                          aTGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGtta                                                                                                                                                                                                   <  1:492441/250‑1 (MQ=255)                                            gtaaaTCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGttattt                                                                                                                                                                                                >  1:667743/4‑251 (MQ=255)                                                aTCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATcc                                                                                                                                                                                            <  1:94359/251‑1 (MQ=255)                                                    cATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGAt                                                                                                                                                                                                                    >  1:790278/1‑223 (MQ=255)                                                    cATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGAt                                                                                                                                                                                                                    >  1:303309/1‑223 (MQ=255)                                                    cATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGc                                                                                                                                                                                         <  1:556919/250‑1 (MQ=255)                                                        aaaGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGa                                                                                                                                                                                    >  1:563730/1‑251 (MQ=255)                                                              gTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGtcgt                                                                                                                                                                              >  1:482041/1‑248 (MQ=255)                                                              ctCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGtcgt                                                                                                                                                                              >  1:653461/2‑248 (MQ=255)                                                                  tagccGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCTCAACTTTAAAGCCATCAAGTCCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGtcgtggaa                                                                                                                                                                          <  1:218988/247‑8 (MQ=255)                                                                  tagccGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGtcgtggaa                                                                                                                                                                          >  1:655093/5‑244 (MQ=255)                                                                     ccGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAgtg                                                                                                                                                                       <  1:139677/250‑1 (MQ=255)                                                                          gTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTatg                                                                                                                                                                   >  1:721195/1‑248 (MQ=255)                                                                             gAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGAGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATgcgg                                                                                                                                                                >  1:431605/1‑250 (MQ=255)                                                                                            aaaTTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTgg                                                                                                                                                 <  1:553543/250‑1 (MQ=255)                                                                                                                              acaACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGac                                                                                                              <  1:826627/251‑1 (MQ=255)                                                                                                                               caACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGaca                                                                                                             >  1:342681/1‑251 (MQ=255)                                                                                                                                 aCTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGacac                                                                                                            >  1:882184/1‑250 (MQ=255)                                                                                                                                 aCTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGacac                                                                                                            >  1:328400/1‑250 (MQ=255)                                                                                                                                  cTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATAtt                                                                                                                 >  1:233628/1‑244 (MQ=255)                                                                                                                                  cTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACAccg                                                                                                          >  1:836599/1‑251 (MQ=255)                                                                                                                                    ttAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACAccgcc                                                                                                        <  1:247862/251‑1 (MQ=255)                                                                                                                                           cATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGtcatca                                                                                                 >  1:223551/1‑251 (MQ=255)                                                                                                                                            aTCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCatg                                                                                                                                                                                                                      >  1:384382/1‑131 (MQ=255)                                                                                                                                            aTCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGaca                                                                                                             <  1:566258/238‑1 (MQ=255)                                                                                                                                                aGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTa                                                                                            >  1:639976/1‑251 (MQ=255)                                                                                                                                                 gACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTaa                                                                                           >  1:52184/1‑251 (MQ=255)                                                                                                                                                        gTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTgg                                                                                     >  1:169640/1‑250 (MQ=255)                                                                                                                                                                          ggcgAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGGGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGgtgg                                                                  >  1:676035/4‑250 (MQ=255)                                                                                                                                                                                ccGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGa                                                            >  1:827804/1‑251 (MQ=255)                                                                                                                                                                                   cACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACa                                                                                                                                                                                                                                  >  1:243295/1‑82 (MQ=255)                                                                                                                                                                                   cACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGac                                                                                                              >  1:671614/1‑198 (MQ=255)                                                                                                                                                                                   cACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGgtgtaat                                                               <  1:157099/245‑4 (MQ=255)                                                                                                                                                                                        aaTCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAGTCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGc                                                     >  1:451556/1‑250 (MQ=255)                                                                                                                                                                                                 caCCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAg                                                                                                                                                                                  <  1:594688/115‑1 (MQ=255)                                                                                                                                                                                                      cccgcgACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTa                                       >  1:594154/3‑250 (MQ=255)                                                                                                                                                                                                            agccTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTa                                 >  1:142619/3‑250 (MQ=255)                                                                                                                                                                                                                   cGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCaa                          >  1:851316/1‑250 (MQ=255)                                                                                                                                                                                                                             aTTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAg                <  1:564885/250‑1 (MQ=255)                                                                                                                                                                                                                             aTTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAg                <  1:115840/250‑1 (MQ=255)                                                                                                                                                                                                                                gAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGc                                                     <  1:800144/210‑1 (MQ=255)                                                                                                                                                                                                                                    aCGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGttatttat                                                                                                                                                                                              <  1:249125/69‑1 (MQ=255)                                                                                                                                                                                                                                    aCGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGc        <  1:803942/251‑1 (MQ=255)                                                                                                                                                                                                                                     cGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGGCACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCgg         >  1:656870/1‑249 (MQ=255)                                                                                                                                                                                                                                     cGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCgg         >  1:211775/1‑249 (MQ=255)                                                                                                                                                                                                                                     cGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGGGTTGGAGAATCCGGAAATCGGTAAAACCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGGGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGGTCGGc        >  1:441463/1‑250 (MQ=255)                                                                                                                                                                                                                                       ttAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTc      <  1:845510/250‑1 (MQ=255)                                                                                                                                                                                                                                          aaGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGaaa  <  1:842736/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   TGCCGGCAAACACATTTTCTGTGAAAAACCGGTGGATGCCGATGTGAATCGCATTAAAGAAGTCTTAGTCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGTGAACCGCACTTAATCCGCGTTACCTCACGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTATCCAGCGGGATGTTCTTTGACATGACCATTCACGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTGGTAGAAGTGTACGCGGCGGGCGGCGTGCTGGTGAATCCTGAAATCGGCAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCAATCGGTGTGATCGACAACAGCCGCAAAGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAA  >  1/1816364‑1816847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑