Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,816,535	T→C	intergenic (+34635/+18849)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,816,535	0	T	C	100.0%	191.9 / NA	67	intergenic (+34635/+18849)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/32);  total (35/32)

ATCCTGAAATTGATGCTGTGTTGGTTTGTTCTTCCACAAACACCCACGCACCGATTTCCATTGAAGCCGCTCGTGCCGGCAAACACATTTTCTGTGAAAAACCGGTGGATGCCGATGTGAATCGCATTAAAGAAGTCTTAGTCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGTGAACCGCACTTAATCCGCGTTACCTCACGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTATCCAGCGGGATGTTCTTTGACATGACCATTCACGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTGGTAGAAGTGTACGCGGCGGGCGGCGTGCTGGTGAATCCTGAAATCGGCAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGG  >  1/1816291‑1816764                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                      aTCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCg                                                                                                                                                                                                                                 >  1:762072/1‑251 (MQ=255)  tCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCg                                                                                                                                                                                                                                 >  1:109358/1‑250 (MQ=255)   ccTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCa                                                                                                                                                                                                                               >  1:261634/1‑251 (MQ=255)        aaTTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTaa                                                                                                                                                                                                                          >  1:19409/1‑251 (MQ=255)          ttGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATc                                                                                                                                                                                                                        <  1:810283/251‑1 (MQ=255)          ttGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATc                                                                                                                                                                                                                        <  1:271882/251‑1 (MQ=255)             aTGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCcg                                                                                                                                                                                                                      >  1:95593/1‑250 (MQ=255)                     ttGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCt                                                                                                                                                                                                              >  1:307316/1‑250 (MQ=255)                              tcctcCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGAcc                                                                                                                                                                                                     >  1:104962/4‑250 (MQ=255)                                   caccaacgcccACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACg                                                                                                                                                                                               <  1:177208/243‑1 (MQ=255)                                   caccaaCACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACg                                                                                                                                                                                               <  1:31671/247‑1 (MQ=255)                                   caccaaCACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACg                                                                                                                                                                                               <  1:74194/247‑1 (MQ=255)                                      caaCACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCAc                                                                                                                                                                                            <  1:562509/250‑1 (MQ=255)                                         cacCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCAccgc                                                                                                                                                                                         <  1:700079/251‑1 (MQ=255)                                                 cgccGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGa                                                                                                                                                                                  <  1:515912/248‑1 (MQ=255)                                                    cGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATAc                                                                                                                                                                              <  1:787050/251‑1 (MQ=255)                                                    cGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATAc                                                                                                                                                                              <  1:726700/251‑1 (MQ=255)                                                       tttCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGtt                                                                                                                                                                           <  1:613826/251‑1 (MQ=255)                                                       tttCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGtt                                                                                                                                                                           <  1:886349/251‑1 (MQ=255)                                                          ccATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTaaa                                                                                                                                                                        >  1:782928/1‑251 (MQ=255)                                                               gAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGtgt                                                                                                                                                                    >  1:596399/1‑248 (MQ=255)                                                                    cgcccGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCt                                                                                                                                                                                                              >  1:814144/5‑203 (MQ=255)                                                                         gTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCcg                                                                                                                                                                                                                      >  1:738331/1‑190 (MQ=255)                                                                         gTGCCGGCAAACACATTTCCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAATCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATc                                                                                                                                                                                                                        <  1:436171/188‑1 (MQ=255)                                                                          tGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATg                                                                                                                                                         <  1:565819/250‑1 (MQ=255)                                                                                cAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCttt                                                                                                                                                   <  1:303974/250‑1 (MQ=255)                                                                                               cgAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCatg                                                                                                                                   <  1:748338/250‑3 (MQ=255)                                                                                                      ccGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGa                                                                                                                            >  1:516256/1‑251 (MQ=255)                                                                                                      ccGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGa                                                                                                                            >  1:887966/1‑251 (MQ=255)                                                                                                         gTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGAc                                                                                                                                                <  1:42577/228‑1 (MQ=255)                                                                                                         gTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACa                                                                                                                          <  1:374561/250‑1 (MQ=255)                                                                                                         gTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACa                                                                                                                          <  1:674570/250‑1 (MQ=255)                                                                                                             aTGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGc                                                                                                                                                                                              <  1:28313/178‑1 (MQ=255)                                                                                                             aTGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGAt                                                                                                                      >  1:678989/1‑250 (MQ=255)                                                                                                                   aTGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGtta                                                                                                                <  1:492441/250‑1 (MQ=255)                                                                                                                     gtaaaTCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGttattt                                                                                                             >  1:667743/4‑251 (MQ=255)                                                                                                                         aTCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATcc                                                                                                         <  1:94359/251‑1 (MQ=255)                                                                                                                             cATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGAt                                                                                                                                 >  1:303309/1‑223 (MQ=255)                                                                                                                             cATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGAt                                                                                                                                 >  1:790278/1‑223 (MQ=255)                                                                                                                             cATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGc                                                                                                      <  1:556919/250‑1 (MQ=255)                                                                                                                               ttAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGc                                                                                                                                                                                              <  1:188643/160‑1 (MQ=255)                                                                                                                                 aaaGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGa                                                                                                 >  1:563730/1‑251 (MQ=255)                                                                                                                                       gTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGtcgt                                                                                           >  1:482041/1‑248 (MQ=255)                                                                                                                                       ctCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGtcgt                                                                                           >  1:653461/2‑248 (MQ=255)                                                                                                                                           tagccGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCTCAACTTTAAAGCCATCAAGTCCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGtcgtggaa                                                                                       <  1:218988/247‑8 (MQ=255)                                                                                                                                           tagccGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGtcgtggaa                                                                                       >  1:655093/5‑244 (MQ=255)                                                                                                                                             gccGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACg                                                                                                                                                                                               <  1:100882/143‑1 (MQ=255)                                                                                                                                              ccGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAgtg                                                                                    <  1:139677/250‑1 (MQ=255)                                                                                                                                                   gTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTatg                                                                                >  1:721195/1‑248 (MQ=255)                                                                                                                                                      gAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACg                                                                                                                                                                                               <  1:387692/136‑1 (MQ=255)                                                                                                                                                      gAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGAGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATgcgg                                                                             >  1:431605/1‑250 (MQ=255)                                                                                                                                                                     aaaTTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTgg                                                              <  1:553543/250‑1 (MQ=255)                                                                                                                                                                                                       acaACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGac                           <  1:826627/251‑1 (MQ=255)                                                                                                                                                                                                        caACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGaca                          >  1:342681/1‑251 (MQ=255)                                                                                                                                                                                                          aCTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGacac                         >  1:882184/1‑250 (MQ=255)                                                                                                                                                                                                          aCTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGacac                         >  1:328400/1‑250 (MQ=255)                                                                                                                                                                                                           cTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATAtt                              >  1:233628/1‑244 (MQ=255)                                                                                                                                                                                                           cTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACAccg                       >  1:836599/1‑251 (MQ=255)                                                                                                                                                                                                             ttAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACAccgcc                     <  1:247862/251‑1 (MQ=255)                                                                                                                                                                                                                    cATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGtcatca              >  1:223551/1‑251 (MQ=255)                                                                                                                                                                                                                     aTCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCAc                                                                                                                                                                                                                              >  1:197569/1‑42 (MQ=255)                                                                                                                                                                                                                     aTCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAg                                                                                                                                                                       >  1:863922/1‑97 (MQ=255)                                                                                                                                                                                                                     aTCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCatg                                                                                                                                   >  1:384382/1‑131 (MQ=255)                                                                                                                                                                                                                     aTCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGaca                          <  1:566258/238‑1 (MQ=255)                                                                                                                                                                                                                         aGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTa         >  1:639976/1‑251 (MQ=255)                                                                                                                                                                                                                          gACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTaa        >  1:52184/1‑251 (MQ=255)                                                                                                                                                                                                                                 gTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTgg  >  1:169640/1‑250 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                      ATCCTGAAATTGATGCTGTGTTGGTTTGTTCTTCCACAAACACCCACGCACCGATTTCCATTGAAGCCGCTCGTGCCGGCAAACACATTTTCTGTGAAAAACCGGTGGATGCCGATGTGAATCGCATTAAAGAAGTCTTAGTCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGTGAACCGCACTTAATCCGCGTTACCTCACGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTATCCAGCGGGATGTTCTTTGACATGACCATTCACGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTGGTAGAAGTGTACGCGGCGGGCGGCGTGCTGGTGAATCCTGAAATCGGCAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGG  >  1/1816291‑1816764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑