Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,815,687	C→T	intergenic (+33787/+19697)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,815,687	0	C	T	100.0%	217.9 / NA	76	intergenic (+33787/+19697)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/39);  total (37/39)

CGGTAAAAAAGAAGAAACCATCAAAGAATTTATCAAACACCGCGATTTTCTCCATGCCATGGGCGCCAAAGTGATCGGCTGTTCCGAACAAAGCCGTAGTATTCAAGGCACCA‑CCAAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACCGGCATTCAAACCCCGGCAGAAATTGATAAATATATGGCGGTGGTCAATGACGATGTGTATTTGTTATTCGATTCAGGTCACCTCTACTATTCCGAAGGCTCACAAAAAGCCATGCTGGATGTCTTGGAAAAATACATCGATCGCATTTGCCATGTGCATTTGAAAGACGTGCGCGATGAAGTGGTCGCCGACGTAAAAGCCAATAATCTGAGCTTCCTGGAAGGCGTGCGTAAAGGCACCTTTAC  >  1/1815441‑1815932                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      tggTAAAAAAGAGGAAACCATCAAAGAATTTATCAAACATCGTGATTTCCTCCATGCCATGGGCGCCAAAGTGATCGGCTGTTCCGAGCAAAGCCGTAGTATTCAAGGCACCA‑CCAAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCa                                                                                                                                                                                                                                                   >  1:267014/2‑251 (MQ=255)   gTAAAAAAGAGGAAACCATCAAAGAATTTATCAAACATCGTGATTTCCTCCATGCCATGGGCGCCAAAGTGATCGGCTGTTCCGAGCAAAGCCGTAGCATTCAAGGCACCA‑CCAAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCAtt                                                                                                                                                                                                                                                 >  1:820550/1‑251 (MQ=255)           gaggaaACCATCAAAGAATTTATCAAACATCGTGATTTCCTCCATGCCATGGGCGCCAAAGTGATCGGCTGTTCCGAGCAAAGCCGTAGTATTCAAGGCACCA‑CCAAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAAccc                                                                                                                                                                                                                                          >  1:3874/4‑250 (MQ=255)                  ccATCAAAGAATTTATCAAACATCGTGATTTCCTCCATGCCATGGGCGCCAAAGTGTTCGGCTGTTCCGAGCAAAGCCGTAGTATTCAAGGCACCA‑CCAAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTATGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGaa                                                                                                                                                                                                                                  >  1:356082/1‑251 (MQ=255)                  ccATCAAAGAATTTATCAAACATCGTGATTTCCTCCATGCCATGGGCGCCAAAGTGATCGGCTGTTCCGAGCAAAGCCGTAGTATTCAAGGCACCA‑CCAAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGACCTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGaa                                                                                                                                                                                                                                  >  1:427088/1‑251 (MQ=255)                  ccATCAAAGAATTTATCAAACATCGTGATTTCCTCCATGCCATGGGCGCCAAAGTGATCGGCTGTTCCGAGCAAAGCCGTAGTATTCAAGGCACCA‑CCAAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGaa                                                                                                                                                                                                                                  >  1:136754/1‑251 (MQ=255)                          gAATTTATCAAACATCGTGATTTCCTCCATGCCATGGGCGCCAAAGTGATCGGCTGTTCCGAGCAAAGCCGTAGTATTCAAGGCACCA‑CCAAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATaa                                                                                                                                                                                                                          >  1:798089/1‑251 (MQ=255)                           aaTTTATCAAACATCGTGATTTCCTCCATGCCATGGGCGCCAAAGTGATCGGCTGTTCCGAGCAAAGCCGTAGTATTCAAGGCACCA‑CCAAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATa                                                                                                                                                                                                                           <  1:74796/249‑1 (MQ=255)                              ttATCAAACATCGTGATTTCCTCCATGCCATGGGCGCCAAAGTGATCGGCTGTTCCGAGCAAAGCCGTAGTATTCAAGGCACCA‑CCAAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAgtat                                                                                                                                                                                                                      >  1:811075/1‑251 (MQ=255)                               tATCAAACATCGTGATTTCCTCCATGCCATGGGCGCCAAAGTGATCGGCTGTTCCGAGCAAAGCCGTAGTATTCAAGGCACTACCCAAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAgtat                                                                                                                                                                                                                      <  1:437717/251‑1 (MQ=255)                                   aaaCATCGTGATTTCCTCCATGCCATGGGCGCCAAAGTGATCGGCTGTTCCGAGCAAAGCCGTAGTATTCAAGGCACCA‑CCAAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCTAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGc                                                                                                                                                                                                                 <  1:102294/251‑1 (MQ=255)                                     acatcgtgATTTCCTCCATGCCATGGGCGCCAAAGTGATCGGCTGTTCCGAGCAAAGCCGTAGTATTCAAGGCACCA‑CCAAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCgg                                                                                                                                                                                                               <  1:60159/244‑1 (MQ=255)                                      catcgtgATTTCCTCCATGCCATGGGCGCCAAAGTGATCGGCTGTTCCGAGCAAAGCCGTAGTATTCAAGGCACCA‑CCAAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCggg                                                                                                                                                                                                              >  1:749076/7‑250 (MQ=255)                                           tgATTTCCTCCATGCCATGGGCGCCAAAGTGATCGGCTGTTCCGAGCAAAGCCGTAGTATTCAAGGCACCA‑CCAAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCggtggtt                                                                                                                                                                                                          <  1:657186/249‑2 (MQ=255)                                           tgATTTCCTCCATGCCATGGGCGCCAAAGTGATCGGCTGTTCCGAGCAAAGCCGTAGTATTCAAGGCACCA‑CCAAAGCCGCGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCggtggtt                                                                                                                                                                                                          <  1:435533/249‑2 (MQ=255)                                            gATTTCCTCCATGCCATGGGCGCCAAAGTGATCGGCTGTTCCGAGCAAAGCCGTAGTATTCAAGGCACCA‑CCAAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCggtggttaa                                                                                                                                                                                                        >  1:473866/1‑248 (MQ=255)                                            gATTTCCTCCATGCCATGGGCGCCAAAGTGATCGGCTGTTCCGAGCAAAGCCGTAGTATTCAAGGCACCA‑CCAAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCggtggttaa                                                                                                                                                                                                        >  1:894062/1‑248 (MQ=255)                                               ttcctcCATGCCATGGGCGCCAAAGTGATCGGCTGTTCCGAGCAAAGCCGTAGTATTCAAGGCACCA‑CCAAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGa                                                                                                                                                                                                     <  1:859780/248‑1 (MQ=255)                                               ttcctcCATGCCATGGGCGCCAAAGTGATCGGCTGTTCCGAGCAAAGCCGTAGTATTCAAGGCACCA‑CCAAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGa                                                                                                                                                                                                     <  1:491042/248‑1 (MQ=255)                                               ttcctcCATGCCATGGGCGCCAAAGTGATCGGCTGTTCCGAGCAAAGCCGTAGTATTCAAGGCACCA‑CCAAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGa                                                                                                                                                                                                     <  1:829514/248‑1 (MQ=255)                                               ttcctcCATGCCATGGGCGCCAAAGTGATCGGCTGTTCCGAGCAAAGCCGTAGTATTCAAGGCACCA‑CCAAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGa                                                                                                                                                                                                     <  1:452624/248‑1 (MQ=255)                                                tcctcCATGCCATGGGCGCCAAAGTGATCGGCTGTTCCGAGCAAAGCCGTAGTATTCAAGGCACCA‑CCAAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGat                                                                                                                                                                                                    <  1:31322/249‑2 (MQ=255)                                                  ctcCATGCCATGGGCGCCAAAGTGATCGGCTGTTCCGAGCAAAGCCGTAGTATTCAAGGCACCA‑CCAAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGatga                                                                                                                                                                                                  >  1:498851/1‑248 (MQ=255)                                                       tgccatgGGCGCCAAAGTGATCGGCTGTTCCGAGCAAAGCCGTAGTATTCAAGGCACCA‑CCAAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATgtgt                                                                                                                                                                                             >  1:680701/1‑251 (MQ=255)                                                                       gTGATCGGCTGTTCCGAGCAAAGCCGTAGTATTCAAGGCACCA‑CCAAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCaa                                                                                                                                                                                                                                              >  1:584191/1‑186 (MQ=255)                                                                         gATCGGCTGTTCCGAGCAAAGCCGTAGTATTCAAGGCACCA‑CCAAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAAcc                                                                                                                                                                                                                                           >  1:362569/1‑187 (MQ=255)                                                                         gATCGGCTGTTCCGAGCAAAGCCGTAGTATTCAAGGCACCA‑CCAAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGTGTATTTGTTATTCGATTCAg                                                                                                                                                                           >  1:573342/1‑251 (MQ=255)                                                                            cGGCTGTTCCGAGCAAAGCCGTAGTATTCAAGGCACCA‑CCAAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGTGTATTTGTTATTCGATTCAGGt                                                                                                                                                                         >  1:609113/1‑250 (MQ=255)                                                                                 gTTCCGAGCAAAGCCGTAGTATTCAAGGCACCA‑CCAAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGGGTATTTGTTATTCGATTCAGGTCatctt                                                                                                                                                                   >  1:380409/1‑247 (MQ=255)                                                                                     cgagcAAAGCCGTAGTATTCAAGGCACCA‑CCAAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGTGTATTTGTTATTCGATTCAGGTCATCTctact                                                                                                                                                               <  1:733246/247‑1 (MQ=255)                                                                                             gCCGTAGTATTCAAGGCACCA‑CCAAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGa                                                                                                                                                                                                     <  1:188398/205‑1 (MQ=255)                                                                                              ccGTAGTATTCAAGGCACCA‑CCAAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGTGTATTTGtt                                                                                                                                                                                      >  1:719785/1‑219 (MQ=255)                                                                                                gtagtaTTCAAGGCACCA‑CCAAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAatgatgatg                                                                                                                                                                                                >  1:844801/1‑207 (MQ=255)                                                                                                gtagtaTTCAAGGCACCA‑CCAAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGTGTATTTGTTATTCGATTCAgg                                                                                                                                                                          <  1:285006/229‑1 (MQ=255)                                                                                                gtagtaTTCAAGGCACCA‑CCAAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGTGTATTTGTTATTCGATTCAGGTCATCTctac                                                                                                                                                                >  1:789734/1‑239 (MQ=255)                                                                                                 tagtaTTCAAGGCACCA‑CCAAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGTGTATTTGTTATTCGATTCAGGTCATCTCTACTATTCCGAAGGc                                                                                                                                                    <  1:42897/250‑1 (MQ=255)                                                                                                 tagtaTTCAAGGCACCA‑CCAAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGTGTATTTGTTATTCGATTCAGGTCATCTCTACTATTCCGAAGGc                                                                                                                                                    <  1:212818/250‑1 (MQ=255)                                                                                                     aTTCAAGGCACCA‑CCAAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGc                                                                                                                                                                                                                 <  1:554480/185‑1 (MQ=255)                                                                                                             cacca‑cCAAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGTGTATTTGTTATTCGATTCAGGTCATCTCTACTATTCCGAAGGCTCACAAAAAGcc                                                                                                                                        <  1:59640/250‑1 (MQ=255)                                                                                                               cca‑ccaAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGc                                                                                                                                                                                                                 <  1:766979/175‑1 (MQ=255)                                                                                                               cca‑ccaAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGTGTATTTGTTATTCGATTCAGGTCATCTCTACTATTCCGAAGGCTCACAAAAAGCCATg                                                                                                                                     >  1:556626/1‑251 (MQ=255)                                                                                                                 a‑ccaAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCACCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGAtgtg                                                                                                                                                                                              <  1:816494/192‑1 (MQ=255)                                                                                                                   ccaAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGTGTATTTGTTATTCGATTCAGGTc                                                                                                                                                                        >  1:203781/1‑213 (MQ=255)                                                                                                                        gCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGTGTATTTGTTATTCGATTCAGGTCATCTCTACTATTc                                                                                                                                                           <  1:522121/221‑1 (MQ=255)                                                                                                                           gtgtTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGTGTATTTGTTATTCGATTCAGGTCATCTCTACTATTCCGAAGGCTCACAAAAAGCCATGCTGGATGtttt                                                                                                                          >  1:883978/1‑248 (MQ=255)                                                                                                                            tgtTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGTGTATTTGTTATTCGATTCAGGTCATCTCTACTATTCCGAAGGCTCACAAAAAGCCATGCTGGATGTTTTg                                                                                                                         <  1:181689/251‑1 (MQ=255)                                                                                                                             gtTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGTGTATTTGTTATTCGATTCAgg                                                                                                                                                                          <  1:797395/201‑1 (MQ=255)                                                                                                                               tCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGTGTATTTGTTATTCGATTCAGGTCATCTCTACTATTCCGAAGGCTCACAAAAAGCCATGCTGGATGTTTTGGaa                                                                                                                      <  1:460407/251‑1 (MQ=255)                                                                                                                                    gagaAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGa                                                                                                                                                                                                     >  1:751409/1‑167 (MQ=255)                                                                                                                                             gTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGc                                                                                                                                                                                                                 >  1:855176/1‑146 (MQ=255)                                                                                                                                             gTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGTGTATTTGTTATTCGATTCAGGTCATCTCTACTATTCCGAAGGCTCACAAAAAGCCATGCTGGATGTTTTGGAAAAATACatcgatcg                                                                                                        >  1:800780/1‑251 (MQ=255)                                                                                                                                                  cACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGTGTATTTGTTATTCGATTCAGGTCATCTCTACTATTCCGAAGGCTCACAAAAAGCCATGCTGGATGTTTTGGAAAAATACATCGATCGCAttt                                                                                                   >  1:492393/1‑251 (MQ=255)                                                                                                                                                         gaagaaTGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGTGTATTTGTTATTCGATTCAGGTCATCTctacta                                                                                                                                                              <  1:874983/185‑1 (MQ=255)                                                                                                                                                          aagaaTGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGTGTATTTGTTATTCGATTCAGGTCATCTCTACTATTCCGAAGGCTCACAAAAAGCCATGCTGGATGTTTTGGAAAAATACATCGATCGCATTTGCCAcgtg                                                                                           <  1:265587/251‑1 (MQ=255)                                                                                                                                                               tGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGTGTATTTGTTATTCGATTCAGGTCATCTCTACTATTCCGAAGGCTCACAAAAAGCCATGCTGGATGTTTTGGAAAAATACATCGATCGCATTTGCCACGTGCAttt                                                                                      >  1:572084/1‑251 (MQ=255)                                                                                                                                                                     cGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGTGTATTTGTTATTCGATTCAGGTCATCTCTACTATTCCGa                                                                                                                                                        >  1:172528/1‑179 (MQ=255)                                                                                                                                                                     cGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGTGTATTTGTTATTCGATTCAGGTCATCTCTACTATTCCGAAGGCTCACAAAAAGCCATGCTGGATGTTTTGGAAAAATACATCGATCGCATTTGCCACGTGCATTTGAAAGa                                                                                <  1:221182/251‑1 (MQ=255)                                                                                                                                                                                 ggTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGTGTATTTGTTATTCGATTc                                                                                                                                                                             >  1:791698/1‑146 (MQ=255)                                                                                                                                                                                 ggTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGTGTATTTGTTATTCGATTCAGGTCATCTCTACTATTCCGAAGGCTCACAAAAAGCCATGCTGGATGTTTTGGAAAAATACATCGATCGCATTTGcc                                                                                                >  1:109641/1‑223 (MQ=255)                                                                                                                                                                                     aCAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGTGTATTTGTTATTCGATTCAGGTCATCTCTACTATTCCGAAGGCTCACAAAAAGCCATGCTGGATGTTTTGGAAAAATACATCGATCGCATTTGCCa                                                                                               <  1:621216/220‑1 (MQ=255)                                                                                                                                                                                           aaCTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGTGTATTTGTTATTCGATTCAGGTCATCTCTACTATTCCGAAGGCTCACAAAAAGCCATGCTGGATGTTTTGGAAAAATACATCGATCGCATTTGCCa                                                                                               <  1:796351/214‑1 (MQ=255)                                                                                                                                                                                                     aCTTGCCGCCGAAAAAGGCAGAAAAGTTTGCCTGCGCCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGTGTATTTGTTATTCGATTCAGGTCATCTCTACTATTCCGAAGGCTCACAAAAAGCCATGCTGGATGTTTTGGAAAAATACATCGATCGCAGTTGCCGCGTGCATTTGAAAGACGTGCGCGATGAAGTGGTCGCCGACGTAAAAg                                                <  1:561401/251‑1 (MQ=255)                                                                                                                                                                                                       ttGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGTGTATTTGTTATTCGATTCAGGTCATCTCTACTATTCCGAAGGCTCACAAAAAGCCATGCTGGATGTTTTGGAAAAATACATCGATCGCATTTGCCACGTGCATTTGAAAGACGTGCGCGATGAAGTGGTCGCCGACGTAAAAGcc                                              <  1:767334/251‑1 (MQ=255)                                                                                                                                                                                                       ttGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGTGTATTTGTTATTCGATTCAGGTCATCTCTACTATTCCGAAGGCTCACAAAAAGCCATGCTGGATGTTTTGGAAAAATACATCGATCGCATTTGCCACGTGCATTTGAAAGACGTGCGCGATGAAGTGGTCGCCGACGTAAAAGcc                                              <  1:459251/251‑1 (MQ=255)                                                                                                                                                                                                             ccgAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGTGTATTTGTTATTCGATTCAGGTCATCTCTACTATTCCGAAGGCTCACAAAAAGCCATGCTGGAt                                                                                                                               <  1:437259/164‑1 (MQ=255)                                                                                                                                                                                                              cgAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGTGTATTTGTTATTCGATTCAGGTCATCTCTACTATTCCGAAGGCTCACAAAAAGCCATGCTGGATGTTTTGGAAAAATACATCGATCGCATTTGCCACGTGCATTTGAAAGACGTGCGCGATGAAGTGGTCGCCGACGTAAAAGCCAATAATc                                       <  1:170645/251‑1 (MQ=255)                                                                                                                                                                                                                      gCATGAAAGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGTGTATTTGTTATTCGATTCAGGTCATCTCTACTATTCCGAAGGCTCACAAAAAGCCATGCTGGATGTTTTGGAAAAATACATCGATCGCATTTGCCACGTGCATTTGAAAGACGTGCGCGATGAAGTGGTCGCCGACGTAAAAGCCAATAATCTGAGCtt                                >  1:566346/1‑250 (MQ=255)                                                                                                                                                                                                                           aaaGTTTGCCTGCACCACCACATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGTGTATTTGTTATTCGATTCAGGTCATCTCTACTATTCCGAAGGCTCACAAAAAGCCATGCTGGATGTTTTGGAAAAATACATCGATCGCATTTGCCACGTGCATTTGAAAGACGTGCGCGATGAAGTGGTCGCCGACGTAAAAGCCAATAATCTGAGCTTCCTgg                           <  1:670706/250‑1 (MQ=255)                                                                                                                                                                                                                                       caccaccacATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGTGTATTTGTTATTCGATTCAGGTCATCTCTACTATTCCGAAGGCTCACAAAAAGCCATGCTGGATGTTTTGGAAAAATACATCGATCGCATTTGCCACGTGCa                                                                                         >  1:113782/1‑176 (MQ=255)                                                                                                                                                                                                                                       caccaccacATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGTGTATTTGTTATTCGATTCAGGTCATCTCTACTATTCCGAAGGCTCACAAAAAGCCATGCTGGATGTTTTGGAAAAATACATCGATCGCATTTGCCACGTGCATTTGAAAGACGTGCGCGATGAAGTGGTCGCCGACGTAAAAGCCAATAATCTGAGCTTCCTGGAAgg                       <  1:154254/242‑1 (MQ=255)                                                                                                                                                                                                                                       caccaccacATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGTGTATTTGTTATTCGATTCAGGTCATCTCTACTATTCCGAAGGCTCACAAAAAGCCATGCTGGATGTTTTGGAAAAATACATCGATCGCATTTGCCACGTGCATTTGAAAGACGTGCGCGATGAAGTGGTCGCCGACGTAAAAGCCAATAATCTGAGCTTCCTGGAAGGCGTGCGTaa              >  1:901170/1‑251 (MQ=255)                                                                                                                                                                                                                                         ccaccacATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGTGTATTTGTTATTCGATTCAGGTCATCTCTACTATTCCGAAGGCTCACAAAAAGCCATGCTGGATGTTTTGGAAAAATACATCGATCGCATTTGCCACGTGCATTTGAAAGACGTGCGCGATGAAg                                                                  >  1:617320/1‑197 (MQ=255)                                                                                                                                                                                                                                            ccacATGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGTGTATTTGTTATTCGATTCAGGTCATCTCTACTATTCCGAAGGCTCACAAAAAGCCATGCTGGATGTTTTGGAAAAATACATCGATCGCATTTGCCACGTGCATTTGAAAGACGTGCGCGATGAAGTGGTCGCCGACGTAAAAGCCAATAATCTGAGCTTCCTGGAAGGCGTGCGTAAAGGCa         >  1:74544/1‑251 (MQ=255)                                                                                                                                                                                                                                               caTGGGCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGTGTATTTGTTATTCGATTCAGGTCATCTCTACTATTCCGAAGGCTCACAAAAAGCCATGCTGGATGTTTTGGAAAAATACATCGATCGCATTTGCCACGTGCATTTGAAAGACGTGCGCGATGAAGTGGTCGCCGACGTAAAAGCCAATAATCTGAGCTTCCTGGAAg                        <  1:123606/233‑1 (MQ=255)                                                                                                                                                                                                                                                    gCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGTGTATTTGTTATTCGATTCAGGTCATCTCTACTATTCCGAAGGCTCACAAAAAGCCATGCTGGATGTTTTGGAAAAATACATCGATCGCATTTGCCACGTGCATTTg                                                                                     <  1:453265/167‑1 (MQ=255)                                                                                                                                                                                                                                                    gCACTGGCATTCAAACCCCGGCAGAAATTGATAAGTATATGGCGGTGGTTAATGATGATGTGTATTTGTTATTCGATTCAGGTCATCTCTACTATTCCGAAGGCTCACAAAAAGCCATGCTGGATGTTTTGGAAAAATACATCGATCGCATTTGCCACGTGCATTTGAAAGACGTGCGCGATGAAGTGGTCGCCGACGTAAAAGCCAATAATCTGAGCTTCCTGGAAGGCGTGCGTAAAGGCACCTTTAc  <  1:556037/250‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      CGGTAAAAAAGAAGAAACCATCAAAGAATTTATCAAACACCGCGATTTTCTCCATGCCATGGGCGCCAAAGTGATCGGCTGTTCCGAACAAAGCCGTAGTATTCAAGGCACCA‑CCAAAGCCGTGTTCAAAGAGAAAACCGTATTCACCGAAGAAGAATGGCAACGCCTCGCCGAAGGTTACAACGAACTTGCCAAACTTGCCGCCGAAAAAGGCATGAAAGTTTGCCTGCACCACCACATGGGCACCGGCATTCAAACCCCGGCAGAAATTGATAAATATATGGCGGTGGTCAATGACGATGTGTATTTGTTATTCGATTCAGGTCACCTCTACTATTCCGAAGGCTCACAAAAAGCCATGCTGGATGTCTTGGAAAAATACATCGATCGCATTTGCCATGTGCATTTGAAAGACGTGCGCGATGAAGTGGTCGCCGACGTAAAAGCCAATAATCTGAGCTTCCTGGAAGGCGTGCGTAAAGGCACCTTTAC  >  1/1815441‑1815932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑