Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,813,638	G→A	intergenic (+31738/+21746)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,813,638	0	G	A	100.0%	223.2 / NA	75	intergenic (+31738/+21746)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/46);  total (29/46)

ATGAACAAGGCATGGCACATGTCGCCATCGGTTTTGCGAAACAAAATCTGCGTAAAAAAATTTACGCCTGCACCTCTTCCGTCGGTCCCGGCGCCGCCAACATGATTACTGCCGCCGCCACCGCAACCGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCGTTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTGGTGTTAACGGATCCGGCGGACACCGGCGCCGTCACCATCGCTCTGCCGCAAGACGTCCAAGCGGAAGCCTATGATTTCCCGGAATATTTCCTGCAAAAACGCATTCACCGCATTGAGCGCACGCTCCCTACCGAACCTATGTTGAAATCTGCCGTGGATTTGATTC  >  1/1813394‑1813882                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     aTGAACAAGGCATGGCACATGTCGCCATCGGTTTTGCGAAACAAAATCTGCGTAAAAAAATTTACGCCTGCACCTCTTCCGTCGGTCCCGGCGCCGCCAACATGATTACTGCCGCCGCCACCGCAACCGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTccgt                                                                                                                                                                                                                                                >  1:286325/1‑251 (MQ=255)   gAACAAGGCATGGCACATGTCGCCATCGGTTTTGCGAAACAAAATCTGCGTAAAAAAATTTACGCCTGCACCTCTTCCGTCGGTCCCGGCGCCGCCAACATGATTACTGCCGCCGCCACCGCAACCGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTccgtg                                                                                                                                                                                                                                               >  1:113382/1‑250 (MQ=255)   gAACAAGGCATGGCACATGTCGCCATCGGTTTTGCGAAACAAAATCTGCGTAAAAAAATTTACGCCTGCACCTCTTCCGTCGGTCCCGGCGCCGCCAACATGATTACTGCCGCCGCCACCGCAACCGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTccgtg                                                                                                                                                                                                                                               >  1:887008/1‑250 (MQ=255)   gAACAAGGCATGGCACATGTCGCCATCGGTTTTGCGAAACAAAATCTGCGTAAAAAAATTTACGCCTGCACCTCTTCCGTCGGTCCCGGCGCCGCCAACATGATTACTGCCGCCGCCACCGCAACCGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTccgtg                                                                                                                                                                                                                                               >  1:199001/1‑250 (MQ=255)    aaCAAGGCATGGCACATGCCGCCATCGGTTTTGCGAAACAAAACCTGCGAAAAAAAATTTCCGCCTGCCCCTCTCCCGTCGGTCCCGGCGCCGCCAACATGATTACTGCCGCCGCCCCCGCAACCGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTccgtgc                                                                                                                                                                                                                                              <  1:44114/250‑1 (MQ=255)    aaCAAGGCAGGGCACATGTCGCCATCGTTTTTGCGAAACAAAATCGGCGTAAAAAAATTTCCGCCTGCCCCTCTTCCGTCGGTCCCGGCGCCGCAAACATGATTCCTGCCGCCGCCCCCGCAACCGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTccgtgc                                                                                                                                                                                                                                              <  1:93461/250‑1 (MQ=255)         ggCATGGCACATGTCGCCATCGGTTTTGCGAAACAAAATCTGCGTAAAAAAATTTACGCCTGCACCTCTTCCGTCGGTCCCGGCGCCGCCAACATGATTACTGCCGCCGCCACCGCAACCGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAg                                                                                                                                                                                                                                        >  1:615968/1‑251 (MQ=255)                cacaTGTCGCCATCGGTTTTGCGAAACAAAATCTGCGTAAAAAAATTTCCGCCTGCACCTCTTCCGTCGGTCCCGGCGCCGCCAACATGATTACTGCCGCCGCCACCGCAACCGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAata                                                                                                                                                                                                                                  <  1:196313/250‑1 (MQ=255)                  caTGTCGCCATCGGTTTTGCGAAACAAAATCTGCGTAAAAAAATTTACGCCTGCACCTCTTCCGTCGGTCCCGGCGCCGCCAACATGATTACTGCTGCCGCCACCGCAACCGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTg                                                                                                                                                                                                                               >  1:164512/1‑251 (MQ=255)                  caTGTCGCCATCGGTTTTGCGAAACAAAATCTGCGTAAAAAAATTTACGCCTGCACCTCTTCCGTCGGTCCCGGCGCCGCCAACATGATTACTGCCGCCGCCACCGCAACCGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGCTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGAGAGCAAATATTg                                                                                                                                                                                                                               >  1:871058/1‑251 (MQ=255)                      ccGCCATCGGTTTTGCGAAACAAAACCTGCGTAAAAAAATTTACGCCTGCCCCTCTCCCGTCGGTCCCGGCGCCGCCAACATGATTCCTGCCGCCGCCCCCGCAACCGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGAc                                                                                                                                                                                                                           <  1:203840/250‑1 (MQ=255)                      ccGCCATCGGTTTTGCGAAACAAAACCTGCGAAAAAAAATTCCCGCCTGCCCCTCTCCCGTCGGTCCCGGCGCCGCAAACATGATTACTGCCGCCGCCCCCGCAACCGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGAc                                                                                                                                                                                                                           <  1:3691/250‑1 (MQ=255)                      ccGCCATCGGTTTGGCGAAACAAAATCTGCGAAAAAAAATTTACGCCTGCACCTCTCCCGTCGGTCCCGGCGCCGCCAACATGATTACGGCCGCCGCCCCCGCAACCGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGAc                                                                                                                                                                                                                           <  1:650139/250‑1 (MQ=255)                           aTCGTTTTTGCGAAACAAAATCTGCGTAAAAAAATTTCCGCCTGCCCCTCTTCCGTCGGTCCCGGCGCCGCCAACATGATTACTGCCGCCGCCACCGCAACCGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGt                                                                                                                                                                                                                      <  1:824969/251‑1 (MQ=255)                           aTCGGTTTTGCGAAACAAAATCTGCGTAAAAAAATTTCCGCCTGCCCCTCTCCCGTCGGTCCCGGCGCCGCCAACATGATTACTGCCGCCGCCACCGCAACCGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGt                                                                                                                                                                                                                      <  1:265169/251‑1 (MQ=255)                               gTTTTGCGAAACAAAATCTGCGTAAAAAAATTTACGCCTGCACCTCTTCCGTCGGTCCCGGCGCCGCCAACATGATTACTGCCGCCGCCACCGCAACCGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAg                                                                                                                                                                                                                   >  1:92629/1‑250 (MQ=255)                                         aCAAAATCTGCGTAAAAAAATTTACGCCTGCACCTCTTCCGTCGGTCCCGGCGCCGCCAACATGATTACTGCCGCCGCCACCGCAACCGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGaa                                                                                                                                                                                                         >  1:124/1‑250 (MQ=255)                                            aaaTCTGCGTAAAAAAATTTACGCCTGCACCTCTTCCGTCGGTCCCGGCGCCGCCAACATGATTACTGCCGCCGCCACCGCAACCGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGaacaa                                                                                                                                                                                                      >  1:566581/1‑250 (MQ=255)                                             aaTCTGCGAAAAAAAGTTTACGCCTCCCCCTCTGCCGCCGGTCCCGGCCCCGCCAACAGGTTTACGCCCGCCGCCACCCCAACCGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACt                                                                                                                                                                                                    <  1:830840/251‑1 (MQ=255)                                                   cGTAAAAAAATTTACGCCTGCACCTCTTCCGTCGGTCCCGGCGCCGCCAACATGATTACTGCCGCCGCCACCGCAACCGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGAc                                                                                                                                                                                              <  1:192855/251‑1 (MQ=255)                                                           aaTTTCCGCCTGCACCTCTTCCGTCGGTCCCGGCGCCGCCAACATGATTACTGCCGCCGCCACCGCAACCGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTa                                                                                                                                                                                      <  1:690066/251‑1 (MQ=255)                                                           aaTTTACGCCTGCCCCTCTTCCGTCGGTCCCGGCGCCGCCAACATGATTACTGCCGCCGCCCCCGCAACCGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTa                                                                                                                                                                                      <  1:761088/251‑1 (MQ=255)                                                            ttttccGCCTGCCCCTCTTCCGTCGGTCCCGGCCCCGCCAACATGATTCCTGCCGCCGCCCCCGCAACCGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTAt                                                                                                                                                                                     <  1:241619/246‑1 (MQ=255)                                                              ttACGCCTGCCCCTCTCCCGTCGGTCCCGGCCCCGCCAACATGATTACTGCCGCCGCCACCGCAACCGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTa                                                                                                                                                                                   <  1:894664/251‑1 (MQ=255)                                                                  cccTGCCCCTCTCCCGTCGGTCCCGGCGCCGCCAACATGATTACTGCCGCCGCCACCGCAACCGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGc                                                                                                                                                                               <  1:250608/250‑1 (MQ=255)                                                                  cccTGCCCCTCTCCCGTCGGTCCCGGCGCCGCCAACATGATTACTCCCGCCGCCCCCGCAACCGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGc                                                                                                                                                                               <  1:131683/250‑1 (MQ=255)                                                                  cccTGCACCTCTTCCGTCGGTCCCGGCCCCGCCAACATGATTCCTGCCGCCGCCACCGCAACCGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGc                                                                                                                                                                               <  1:252372/250‑1 (MQ=255)                                                                   ccTGCCCCTCTCCCGTCGGTCCCGGCGCCGCCAACATGATTACTGCCGCCGCCACCGCAACCGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGc                                                                                                                                                                               <  1:854970/250‑1 (MQ=255)                                                                       ccccTCTTCCGTCGGTCCCGGCGCCGCCAACATGATTACTGCCGCCGCCCCCGCAACCGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTccgtgccg                                                                                                                                                                                                                                            <  1:382042/183‑1 (MQ=255)                                                                            ctTCCGTCGGTCCCGGCGCCGCCAACATGATTACTGCCGCCGCCACCGCAACCGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTGgtg                                                                                                                                                                     <  1:512557/251‑1 (MQ=255)                                                                                                cccAACATGATTACTGCCGCCGCCACCGCAACCGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTGGTGTTAACGGATCCGGCGGacac                                                                                                                                                 <  1:669985/250‑1 (MQ=255)                                                                                                ccaaccatgttaccTGCCCCCCCCACCGCACCCGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTGGTGTTAACGGATCCGGCGGacac                                                                                                                                                 <  1:539069/238‑1 (MQ=255)                                                                                                 ccAACATGATTCCTGCCCCCGCCCCCGCAACCGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTGGTGTTAACGGATCCGGCGGACAcc                                                                                                                                                <  1:108444/251‑1 (MQ=255)                                                                                                     cATGATTACTGCCGCCGCCCCCGCAACCGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTccgtgcc                                                                                                                                                                                                                                             <  1:892314/154‑1 (MQ=255)                                                                                                       tGATTACTGCCGCCGCCACCGCAACCGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACACATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACg                                                                                                                                                                                >  1:218938/1‑213 (MQ=255)                                                                                                        gATTACTGCCGCCGCCACCGCAACCGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTa                                                                                                                                                                                   >  1:386084/1‑209 (MQ=255)                                                                                                               cccgccgccACCGCACCCGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTGGTGTTAACGGATCCGGCGGACACCGGCGCCGTCACCAt                                                                                                                                  <  1:615595/250‑1 (MQ=255)                                                                                                                  cccgccCCCGCAACCGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTGGTGTTAACGGATCCGGCGGACACCGGCGCCGTCACCATCGc                                                                                                                               <  1:745141/250‑1 (MQ=255)                                                                                                                    cccccccGCAACCGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTGGTGTTAACGGATCCGGCGGACACCGGCGCCGTCACCATCGctc                                                                                                                             <  1:897896/246‑1 (MQ=255)                                                                                                                       ccccGCAACCGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTgg                                                                                                                                                                       <  1:561622/204‑1 (MQ=255)                                                                                                                               ccGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTGGTGTTAACGGATCCGGCGGACACCGGCGCCGTCACCATCGCTCTGCCGCAAGa                                                                                                                   >  1:579288/1‑250 (MQ=255)                                                                                                                                     aTCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTGGTGTTAACg                                                                                                                                                               <  1:343887/200‑1 (MQ=255)                                                                                                                                         cATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTGGTGTTAACGGATCCGGCGGACACCGGCGCCGTCACCATCGCTCTGCCGCAAGACGTCCAAGCgg                                                                                                        <  1:173520/251‑1 (MQ=255)                                                                                                                                           ttCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTGGTGTTAACGGATCCGGCGGACACCGGCGCCGTCACCATCGCTCTGCCGCAAGACGTCCAAGCGGaa                                                                                                      >  1:300508/1‑251 (MQ=255)                                                                                                                                              cGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTGGTGTTAACGGATCCGGCGGACACCGGCGCCGTCACCATCGCTCTGCCGCAAGACGTCCAAGCGGAAGcc                                                                                                   <  1:403119/251‑1 (MQ=255)                                                                                                                                                    ttcttTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTGGTGTTAACGGATc                                                                                                                                                           <  1:55630/189‑1 (MQ=255)                                                                                                                                                    ttcttTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTGGTGTTAACGGATCCGGCGGACACCGGCGCCGTCACCATCGCTCTGCCGCAAGACGTCCAAGCGGAAGCCTATGAt                                                                                             >  1:169433/1‑251 (MQ=255)                                                                                                                                                    ttcttTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTGGTGTTAACGGATCCGGCGGACACCGGCGCCGTCACCATCGCTCTGCCGCAAGACGTCCAAGCGGAAGCCTATGAt                                                                                             <  1:547511/251‑1 (MQ=255)                                                                                                                                                     tcttTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTGGTGTTAACGGATCcgg                                                                                                                                                        >  1:314209/1‑191 (MQ=255)                                                                                                                                                      cttTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTGGTGTTAACGGATCCGGCGGACACCGGCGCCGTCAc                                                                                                                                     >  1:305642/1‑209 (MQ=255)                                                                                                                                                              gggTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTGGTGTTAACGGATCCGGCGGACACCGGCGCCGTCACCATCGct                                                                                                                              >  1:43240/1‑208 (MQ=255)                                                                                                                                                                    tGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTGGTGTTAACGGATCCGGCGGACACCGGCGCCGTCACCATCGCTCTGCCGCAAGACGTCCAAGCGGAAGCCTATGATTTCCCGGAATATTTcc                                                                             <  1:253287/251‑1 (MQ=255)                                                                                                                                                                               cccGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTGGTGTTAACGGATCCGGCGGACACCGGCGCCGTCACCATCGCTCTGCCGCAAGACGTCCAAGCGGAAGcc                                                                                                   <  1:345129/218‑1 (MQ=255)                                                                                                                                                                               cccGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTGGTGTTAACGGATCCGGCGGACACCGGCGCCGTCACCATCGCTCTGCCGCAAGACGTCCAAGCGGAAGCCTATGATTTCCCGGAATATTTCCTGCAAAAACg                                                                   >  1:844156/1‑250 (MQ=255)                                                                                                                                                                                ccGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTGGTGTTAACGGATCCGGCGGACACCGGCGCCGTCACCATCGCTCTGCCGCAAGACGTCCAAGCGGAAGCCTATGATTTCCCGGAATATTTCCTGCAAAAACGCa                                                                 >  1:456751/1‑251 (MQ=255)                                                                                                                                                                                 cGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGc                                                                                                                                                                               >  1:683700/1‑140 (MQ=255)                                                                                                                                                                                 cGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTGGTGTTAACGGATCCGGCGGACACCGGCGCCGTCACCATCGCTCTGCCGCAAGACGTCCAAGCGGAAGCCTATGATTTCCCGGAATATTTCCTGCAAAAACGCAt                                                                >  1:878656/1‑251 (MQ=255)                                                                                                                                                                                     aaCCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTGGTGTTAGCGGATCCGGCGGACACCGGCGCCGTCACCATCGCTCTGCCGCAAGACGTCCAAGCGGAAGCCTATGATTTCCCGGAATATTTCCTGCAAAAACGCATTCAc                                                            <  1:880565/251‑1 (MQ=255)                                                                                                                                                                                       ccGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTGGTGTTAACGGATCCGGCGGACACCGGCGCCGTCACCATCGCTCTGCCGCAAGACGTCCAAGCGGAAGCCTATGAt                                                                                             <  1:214462/216‑1 (MQ=255)                                                                                                                                                                                              cGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTGGTGTTAACGGATCCGGCGGACACCGGCGCCGTCACCATCGctc                                                                                                                             >  1:707024/1‑177 (MQ=255)                                                                                                                                                                                              cGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTGGTGTTAACGGATCCGGCGGACACCGGCGCCGTCACCATCGCTCTGCCGCAAGACGTCCAAGCGGAAGCCTATGATTTCCCGGAATATTTCCTGCAAAAACGCATTCACCGCATTGAg                                                   <  1:385077/251‑1 (MQ=255)                                                                                                                                                                                                gTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTGGTGTTAACGGATCCGGCGGACACCGGCGCCGTCACCATCGCTCTGCCGCAAGACGTCCAAGCGGAAGCCTATGATTTCCCGGAATATTTCCTGCAAAAACGCATTCACCGCATTGAgcg                                                 >  1:288223/1‑251 (MQ=255)                                                                                                                                                                                                            aTCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTGGTGTTAACGGATCCGGCGGACACCGGCGCCGTCACCATCGCTCTGCCGCAAGACGTCCAAGCGGAAGcc                                                                                                   >  1:127036/1‑189 (MQ=255)                                                                                                                                                                                                            aTCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTGGTGTTAACGGATCCGGCGGACACCGGCGCCGTCACCATCGCTCTGCCGCAAGACGTCCAAGCGGAAGCCTATGATTTCCCGGAATATTTCCTGCAAAAACGCATTCACCGCATTGAGCGCACGCTCCCTAc                                     <  1:739494/251‑1 (MQ=255)                                                                                                                                                                                                            aTCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTGGTGTTAACGGATCCGGCGGACACCGGCGCCGTCACCATCGCTCTGCCGCAAGACGTCCAAGCGGAAGCCTATGATTTCCCGGAATATTTCCTGCAAAAACGCATTCACCGCATTGAGCGCACGCTCCCTAc                                     <  1:660953/251‑1 (MQ=255)                                                                                                                                                                                                                    aTTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGcc                                                                                                                                                                                                                 <  1:761918/71‑1 (MQ=255)                                                                                                                                                                                                                    aTTCCATGATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTGGTGTTAACGGATCCGGCGGACACCGGCGCCGTCACCATCGCTCTGCCGCAAGACGTCCAAGCGGAAGCCTATGATTTCCCGGAATATTTCCTGCAAAAACGCATTCACCGCATTGAGCGCACGCTCCCTACCGa                                  <  1:179470/246‑1 (MQ=255)                                                                                                                                                                                                                           gATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTGGTGTTAACGGATCCGGCGGACACCGGCGCCGTCACCATCGCTCTGCCGCAAGACGTCCAAGCGGAAGCCTATGATTTCCCGGAATATTTCCTGCAAAAACGCATTCACCGCATTGAGCGCACGCTCCCTACCGAACCTATGTTg                        >  1:324875/1‑249 (MQ=255)                                                                                                                                                                                                                           gATTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTGGTGTTAACGGATCCGGCGGACACCGGCGCCGTCACCATCGCTCTGCCGCAAGACGTCCAAGCGGAAGCCTATGATTTCCCGGAATATTTCCTGCAAAAACGCATTCACCGCATTGAGCGCACGCTCCCTACCGAACCTATGTTGaa                      >  1:790053/1‑251 (MQ=255)                                                                                                                                                                                                                            aTTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTGGTGTTAACGGATCCGGCGGACACCGGCGCCGTCACCATCGCTCTGCCGCAAGACGTCCAAGCGGAGGCCTATGATTTCCCGGAATATTTcc                                                                             >  1:448050/1‑195 (MQ=255)                                                                                                                                                                                                                            aTTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTGGTGTTAACGGATCCGGCGGACACCGGCGCCGTCACCATCGCTCTGCCGCAAGACGTCCAAGCGGAAGCCTATGAt                                                                                             <  1:220156/179‑1 (MQ=255)                                                                                                                                                                                                                            aTTTAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTGGTGTTAACGGATCCGGCGGACACCGGCGCCGTCACCATCGCTCTGCCGCAAGACGTCCAAGCGGAAGCCTATGATTTCCCGGAATATTTCCTGCAAAAACGCATTCACCGCATTGAGCGCACGCTCCCTACCGAACCTATGTTGaaa                     <  1:850070/251‑1 (MQ=255)                                                                                                                                                                                                                              ttAAGCATCAGCACAAACGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTGGTGTTAACGGATCCGGCGGACACCGGCGCCGTCACCATCGCTCTGCCGCAAGACGTCCAAGCGGAAGCCTATGATTTCCCGGAATATTTCCTGCAAAAACGCATTCACCGCATTGAGCGCACGCTCCCTACCGAACCTATGTTGAAATc                   <  1:326386/251‑1 (MQ=255)                                                                                                                                                                                                                                             aaCGACGCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTGGTGTTAACGGATCCGGCGGACACCGGCGCCGTCACCATCGCTCTGCCGCAAGACGTCCAAGCGGAAGCCTATGATTTCCCGGAATATTTCCTGCAAAAACGCATTCACCGCATTGAGCGCACGCTCCCTACCg                                   >  1:356113/1‑220 (MQ=255)                                                                                                                                                                                                                                               cgacgCATTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTGGTGTTAACGGATCCGGCGGACACCGGCGCCGTCACCATCGCTCTGCCGCAAGACGTCCAAGCGGAAGCCTATGATTTCCCGGAATATTTCCTGCAAAAACGCATTCACCGCATTGAGCGCACGCTCCCTACCGAACCTATGTTGAAATCTGCCGTGGATTTGATTc  <  1:583521/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     ATGAACAAGGCATGGCACATGTCGCCATCGGTTTTGCGAAACAAAATCTGCGTAAAAAAATTTACGCCTGCACCTCTTCCGTCGGTCCCGGCGCCGCCAACATGATTACTGCCGCCGCCACCGCAACCGCCAATCGCATTCCGCTTCTTCTTTTACCGGGTGATGTTTTCGCCACCCGCCAACCGGATCCGGTTTTACAACAAATCGAACAATTCCATGATTTAAGCATCAGCACAAACGACGCGTTCCGTGCCGTGAGCAAATATTGGGACAGAGTCAGCCGTCCGGAACAACTGATGACCGCTTGTATTAACGCCATGCTGGTGTTAACGGATCCGGCGGACACCGGCGCCGTCACCATCGCTCTGCCGCAAGACGTCCAAGCGGAAGCCTATGATTTCCCGGAATATTTCCTGCAAAAACGCATTCACCGCATTGAGCGCACGCTCCCTACCGAACCTATGTTGAAATCTGCCGTGGATTTGATTC  >  1/1813394‑1813882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑