Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,811,169	G→A	intergenic (+29269/+24215)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,811,169	0	G	A	100.0%	207.1 / NA	75	intergenic (+29269/+24215)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/37);  total (38/37)

CAAGCAAGTAACAAAAATGCCCCGAACGGGGCATTTTACGTTGATGGTTATTAACGGGATTTCCAAAGTTTAATCAGTCTTCTGTATCTTTCACCCACTTCGGCGATCAGTTCGTCATCTGTTAATTTTCCGGCAAACCAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCGCTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCGGTAATTTCCACCAATCCGGCTTAATACCTAATTGATAGAAGTGTGCGAGAATGTCCGCGTAATATTGTTCGTTTTGTTCCATATCCGCCGGCAAAATCACTTCCAACAGCAACTCATGACCGGTTCGACAGCAGGC  >  1/1810935‑1811407                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                               caagcaagTAACAAAAATGCCCCGAACGGGGCATTTTACGTTGATGGTTATTAACGGGATTTCCAAAGTTTAATCAGTCTTCTGTATCTTTCACCCACTTCGGCGATCAGTTCGTCATCTGTTAATTTTCCGGCAAACCAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCaa                                                                                                                                                                                                                                >  1:571213/1‑251 (MQ=255)                aaTGCCCCGAACGGGGCATTTTACGTTGATGGTTATTAACGGGATTTCCAAAGTTTAATCAGTCTTCTGTATCTTTCACCCACTTCGGCGATCAGTTCGTCATCTGTTAATTTTCCGGCAAACCAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAAt                                                                                                                                                                                                                 <  1:673171/251‑1 (MQ=255)                  tGCCCCGAACGGGGCATTTTACGTTGATGGTTATTAACGGGATTTCCAAAGTTTAATCAGTCTTCTGTATCTTTCACCCACTTCGGCGATCAGTTCGTCATCTGTTAATTTTCCGGCAAACCAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGc                                                                                                                                                                                                               <  1:241999/251‑1 (MQ=255)                        gAACGGGGCATTTTACGTTGATGGTTATTAACGGGATTTCCAAAGTTTAATCAGTCTTCTGTATCTTTCACCCACTTCGGCGATCAGTTCGTCATCTGTTAATTTTCCGGCAAACCAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGa                                                                                                                                                                                                          >  1:208948/1‑250 (MQ=255)                             gggCATTTTACGTTGATGGTTATTAACGGGATTTCCAAAGTTTAATCAGTCTTCTGTATCTTTCACCCACTTCGGCGATCAGTTCGTCATCTGTTAATTTTCCGGCAAACCAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAatat                                                                                                                                                                                                    >  1:327370/1‑251 (MQ=255)                                 aTTTTACGTTGATGGTTATTAACGGGATTTCCAAAGTTTAATCAGTCTTCTGTATCTTTCACCCACTTCGGCGATCAGTTCGTCATCTGTTAATTTTCCGGCAAACCAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCaaaa                                                                                                                                                                                                                  <  1:692918/233‑1 (MQ=255)                                 aTTTTACGTTGATGGTTATTAACGGGATTTCCAAAGTTTAATCAGTCTTCTGTATCTTTCACCCACTTCGGCGATCAGTTCGTCATCTGTTAATTTTCCGGCAAACCAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGAt                                                                                                                                                                                                >  1:562932/1‑251 (MQ=255)                                  ttttACGTTGATGGTTATTAACGGGATTTCCAAAGTTTAATCAGTCTTCTGTATCTTTCACCCACTTCGGCGATCAGTTCGTCATCTGTTAATTTTCCGGCAAACCAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAACACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATc                                                                                                                                                                                               <  1:256657/251‑1 (MQ=255)                                      aCGTTGATGGTTATTAACGGGATTTCCAAAGTTTAATCAGTCTTCTGTATCTTTCACCCACTTCGGCGATCAGTTCGTCATCTGTTAATTTTCCGGCAAACCAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCAttt                                                                                                                                                                                           >  1:789150/1‑251 (MQ=255)                                              ggTTATTAACGGGATTTCCAAAGTTTAATCAGTCTTCTGTATCTTTCACCCACTTCGGCGATCAGTTCGTCATCTGTTAATTTTCCGGCAAACCAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATc                                                                                                                                                                                               >  1:232014/1‑239 (MQ=255)                                                  attaACGGGATTTCCAAAGTTTAATCAGTCTTCTGTATCTTTCACCCACTTCGGCGATCAGTTCGTCATCTGTTAATTTTCCGGCAAACCAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTa                                                                                                                                                                                 >  1:320590/1‑249 (MQ=255)                                                   ttaACGGGATTTCCAAAGTTTAAGCAGTCTTCTGTATCTTTCACCCACTTCGGCGATCAGTTCGTCATCTGTTAATTTTCCGGCAAACCAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACtt                                                                                                                                                                              <  1:478408/251‑1 (MQ=255)                                                         ggATTTCCAAAGTTTAATCAGTCTTCTGTATCTTTCACCCACTTCGGCGATCAGTTCGTCATCTGTTAATTTTCCGGCAAACCAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTg                                                                                                                                                                         <  1:161146/250‑1 (MQ=255)                                                           aTTTCCAAAGTTTAATCAGTCTTCTGTATCTTTCACCCACTTCGGCGATCAGTTCGTCATCTGTTAATTTTCCGGCAAACCAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATa                                                                                                                                                                      <  1:624084/251‑1 (MQ=255)                                                            tttCCAAAGTTTAATCAGTCTTCTGTATCTTTCACCCACTTCGGCGATCAGTTCGTCATCTGTTAATTTTCCGGCAAACCAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATAc                                                                                                                                                                     <  1:324315/251‑1 (MQ=255)                                                                    gTTTAATCAGTCTTCTGTATCTTTCACCCACTTCGGCGATCAGTTCGTCATCTGTTAATTTTCCGGCAAACCAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCaa                                                                                                                                                             >  1:291327/1‑251 (MQ=255)                                                                             gTCTTCTGTATCTTTCACCCACTTCGGCGATCAGTTCGTCATCTGTTAATTTTCCGGCAAACCAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAatat                                                                                                                                                                                                    >  1:52085/1‑203 (MQ=255)                                                                             gTCTTCTGTATCTTTCACCCACTTCGGCGATCAGTTCGTCATCTGTTAATTTTCCGGCAAACCAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATcc                                                                                                                                                          <  1:65342/245‑1 (MQ=255)                                                                             gTCTTCTGTATCTTTCACCCACTTCGGCGATCAGTTCGTCATCTGTTAATTTTCCGGCAAACCAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATcc                                                                                                                                                          <  1:227480/245‑1 (MQ=255)                                                                               cttctGTATCTTTCACCCACTTCGGCGATCAGTTCGTCATCTGTTAATTTTCCGGCAAACCAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACg                                                                                                                                                                    >  1:519355/1‑233 (MQ=255)                                                                               cttctGTATCTTTCACCCACTTCGGCGATCAGTTCGTCATCTGTTAATTTTCCGGCAAACCAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAAt                                                                                                                                                            <  1:657138/241‑1 (MQ=255)                                                                                ttctGTATCTTTCACCCACTTCGGCGATCAGTTCGTCATCTGTTAATTTTCCGGCAAACCAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTc                                                                                                                                                                 <  1:498018/235‑1 (MQ=255)                                                                                 tctGTATCTTTCACCCACTTCGGCGATCAGTTCGTCATCTGTTAATTTTCCGGCAAACCAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGAt                                                                                                                                                                                                >  1:333835/1‑203 (MQ=255)                                                                                 tctGTATCTTTCACCCACTTCGGCGATCAGTTCGTCATCTGTTAATTTTCCGGCAAACCAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATcc                                                                                                                                                          <  1:551650/241‑1 (MQ=255)                                                                                 tctGTATCTTTCACCCACTTCGGCGATCAGTTCGTCATCTGTTAATTTTCCGGCAAACCAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGaaa                                                                                                                                                >  1:772279/1‑251 (MQ=255)                                                                                      aTCTTTCACCCACTTCGGCGATCAGTTCGTCATCTGTTAATTTTCCGGCAAACCAATCCGCCGACGGCTGCCCGACAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTCCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGcc                                                                                                                                            >  1:887724/1‑250 (MQ=255)                                                                                       tCTTTCACCCACTTCGGCGATCAGTTCGTCATCTGTTAATTTTCCGGCAAACCAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCg                                                                                                                                          >  1:879578/1‑251 (MQ=255)                                                                                                  cTTCGGCGATCAGTTCGTCATCTGTTAATTTTCCGGCAAACCAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTg                                                                                                                                                                                          >  1:314640/1‑192 (MQ=255)                                                                                                            cAGTTCGTCATCTGTTAATTTTCCGGCAAACCAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCGGTAATTTCCACCAATCCgg                                                                                                                       >  1:857669/1‑249 (MQ=255)                                                                                                              gTTCGTCATCTGTTAATTTTCCGGCAAACCAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGcc                                                                                                                                                                                        <  1:396066/182‑1 (MQ=255)                                                                                                                   tCATCTGTTAATTTTCCGGCAAACCAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCGGTAATTTCCACCAATCCGGCTTAATAcc                                                                                                              <  1:98194/251‑1 (MQ=255)                                                                                                                   tCATCTGTTAATTTTCCGGCAAACCAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCGGTAATTTCCACCAATCCGGCTTAATAc                                                                                                               <  1:768633/250‑1 (MQ=255)                                                                                                                   tCATCTGTTAATTTTCCGGCAAACCAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCGGTAATTTCCACCAATCCGGCTTAATAc                                                                                                               <  1:141950/250‑1 (MQ=255)                                                                                                                   tCATCTGTTAATTTTCCGGCAAACCAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCGGTAATTTCCACCAATCCGGCTTAATAc                                                                                                               <  1:144156/250‑1 (MQ=255)                                                                                                                     aTCTGTTAATTTTCCGGCAAACCAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCGGTAA‑TTCCACCAATCCGGCTTAATACCTaa                                                                                                           >  1:142945/1‑251 (MQ=255)                                                                                                                       cTGTTAATTTTCCGGCAAACCAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCGGTAATTTCCACCAATCCGGCTTAATACCTAAt                                                                                                          <  1:571075/251‑1 (MQ=255)                                                                                                                          ttAATTTTCCGGCAAACCAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCGGTAATTTCCACCAATCCGGCTTAATACCTAATTg                                                                                                        <  1:713868/250‑1 (MQ=255)                                                                                                                             aTTTTCCGGCAAACCAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCGGTAATTTCCACCAATCCGGCTTAATACCTAATTGATAg                                                                                                    <  1:63989/251‑1 (MQ=255)                                                                                                                                ttCCGGCAAACCAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCGGTAATTTCCACCAATCCGGCTTAATACCTAATTGATAGAAg                                                                                                 <  1:703471/251‑1 (MQ=255)                                                                                                                                ttCCGGCAAACCAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCGGTAATTTCCACCAATCCGGCTTAATACCTAATTGATAGAAg                                                                                                 <  1:261341/251‑1 (MQ=255)                                                                                                                                        aaCCAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCGGTAATTTCCACCAATCCGGCTTAATACCTAATTGATAGAAg                                                                                                 <  1:18673/243‑1 (MQ=255)                                                                                                                                          ccAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCGGTAATTTCCACCAATCCGGCTTAATAc                                                                                                               >  1:722361/1‑227 (MQ=255)                                                                                                                                            aaTCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCGGTAATTTCCACCAATCCGGCTTAATACCTAATTGATAGAAGTGTGCGAGAATg                                                                                     <  1:213939/251‑1 (MQ=255)                                                                                                                                                 gccgACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGAt                                                                                                                                                                                                >  1:598103/1‑139 (MQ=255)                                                                                                                                                 gccgACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCGGTAATTTCCACCAATCCGGCTTAATAc                                                                                                               <  1:688434/220‑1 (MQ=255)                                                                                                                                                      cGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCGGTAATTTCCACCAATCCGGCTTAATACCTAATTGATAGAAGTGTGCGAGAATGTCCGCGTAat                                                                           >  1:113910/1‑251 (MQ=255)                                                                                                                                                         cTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCGGTAATTTCCACCAATCCGGCTTAATACCTAATTGATAGAAGTGTGCGAGAATGTCCGCGTAatat                                                                         <  1:431781/250‑1 (MQ=255)                                                                                                                                                         cTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCGGTAATTTCCACCAATCCGGCTTAATACCTAATTGATAGAAGTGTGCGAGAATGTCCGCGTAatat                                                                         <  1:345453/250‑1 (MQ=255)                                                                                                                                                                 aaaTTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCGGTAATTTCCACCAATCCGGCTTAATACCTAATTGATAGAAGTGTGCGAGAATGTCCGCGTAATATTGTTCGttt                                                                >  1:574287/1‑251 (MQ=255)                                                                                                                                                                           ccccACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCGGTAATTTCCACCAATCCGGCTTAATACCTAATTGATAGAAGTGTGCGAGAATGTCCGCGTAATATTGTTCGTTTTGTTCCatat                                                      <  1:520547/251‑1 (MQ=255)                                                                                                                                                                           ccccACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCGGTAATTTCCACCAATCCGGCTTAATACCTAATTGATAGAAGTGTGCGAGAATGTCCGCGTAATATTGTTCGTTTTGTTCCatat                                                      >  1:62668/1‑251 (MQ=255)                                                                                                                                                                                cTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGcc                                                                                                                                                                                        >  1:808534/1‑116 (MQ=255)                                                                                                                                                                                cTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCGGTAATTTCCACCAATCCGGCTTAATACCTAATTGATAGAAGTGTGCGAGAATGTCCGCGTAATATTGTTCGTTTTGTTCCATATccgc                                                  >  1:675211/1‑250 (MQ=255)                                                                                                                                                                                cTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCGGTAATTTCCACCAATCCGGCTTAATACCTAATAGATAGAAGTGTGCGAGAATGTCCGCGTAATATTGTTCGTTTTGTTCCATATccgc                                                  >  1:103142/1‑250 (MQ=255)                                                                                                                                                                                       gCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCGGTAATTTCCACCAATCCGGCTTAATACCTAATTGATAGAAGTGTGCGAGAATGTCCGCGTAatat                                                                         >  1:304239/1‑220 (MQ=255)                                                                                                                                                                                       gCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCGGTAATTTCCACCAATCCGGCTTAATACCTAATTGATAGAAGTGTGCGAGAATGTCCGCGTAATATTGTTCGTTTTGTTCCATATCCGCCGGCaaaa                                          >  1:478181/1‑251 (MQ=255)                                                                                                                                                                                       gCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCGGTAATTTCCACCAATCCGGCTTAATACCTAATTGATAGAAGTGTGCGAGAATGTCCGCGTAATATTGTTCGTTTTGTTCCATATCCGCCGGCaaaa                                          >  1:151314/1‑251 (MQ=255)                                                                                                                                                                                         cTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAAt                                                                                                                                                            <  1:273289/135‑1 (MQ=255)                                                                                                                                                                                             tACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCGGTAATTTCCACCAATCCGGCTTAATACCTAATTGATAGAAGTGTGCGAGAATGTCCGCGTAATATTGTTCGTTTTGTTCCATATCCGCCGGCAAAATCACtt                                    >  1:169042/1‑251 (MQ=255)                                                                                                                                                                                                     gCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCGGTAATTTCCACCAATCCGGCTTAATACCTAATTGATAGa                                                                                                   >  1:99385/1‑180 (MQ=255)                                                                                                                                                                                                                 aCGCATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCGGTAATTTCCACCAATCCGGCTTAATACCTAATTGATAGAAGTGTGCGAGAATGTCCGCGTAATATTGTTCGTTTTGTTCCATATCCGCCGGCAAAATCACTTCCAACAGCAACTCATGAc                  <  1:1091/249‑1 (MQ=255)                                                                                                                                                                                                                    cATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGAt                                                                                                                                                                                                >  1:859155/1‑72 (MQ=255)                                                                                                                                                                                                                    cATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCGGTAATTTCCACCAATCCGGCTTAATACCTAATTGATAGAAGTGTGCGAGAATGTCCGCGTAATATTGTTCGTTTTGTTCCATATCCGCCGGCAAAATCACTTCCAACAGCAACTCATGACCGGtt             >  1:372275/1‑251 (MQ=255)                                                                                                                                                                                                                    cATCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCGGTAATTTCCACCAATCCGGCTTAATACCTAATTGATAGAAGTGTGCGAGAATGTCCGCGTAATATTGTTCGTTTTGTTCCATATCCGCCGGCAAAATCACTTCCAACAGCAACTCATGACCGGtt             >  1:149045/1‑251 (MQ=255)                                                                                                                                                                                                                     aTCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCGGTAATTTCCACCAATCCGGCTTAATACCTAATTGATAGAAGTGTGCGAGAATGTCCGCGTAATATTGTTCGTTTTGTTCCATATCCGCCGGCAAAATCACTTCCAACAGCAACTCATGAcc                 >  1:108504/1‑246 (MQ=255)                                                                                                                                                                                                                     aTCAAATACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCGGTAATTTCCACCAATCCGGCTTAATACCTAATTGATAGAAGTGTGCGAGAATGTCCGCGTAATATTGTTCGTTTTGTTCCATATCCGCCGGCAAAATCACTTCCAACAGCAACTCATGACCGGtt             >  1:375932/1‑250 (MQ=255)                                                                                                                                                                                                                        aaaTACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCGGTAATTTCCACCAATCCGGCTTAATACCTAATTGATAGAAGTGTGCGAGAATGTCCGCGTAATATTGTTCGTTTTGTTCCATATCCGCCGGCAAAATCACTTCCAACAGCAACTCATGAcc                 <  1:177235/243‑1 (MQ=255)                                                                                                                                                                                                                        aaaTACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCGGTAATTTCCACCAATCCGGCTTAATACCTAATTGATAGAAGTGTGCGAGAATGTCCGCGTAATATTGTTCGTTTTGTTCCATATCCGCCGGCAAAATCACTTCCAACAGCAACTCATGACCGGTTCGa          >  1:357164/1‑250 (MQ=255)                                                                                                                                                                                                                          aTACTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGAGCATTTGCCTGAATTACTGCGCTGATACGTTCCCAATCCTGCGATGAAGCGCCCGGTAATTTCCACCAATCCGGCTTAATACCTAATTGATAGAAGTGTGCGGGAATGTCCGCGTAATATTGTTCGTTTTGTTCCATATCCGCCGGCAAAATCACTTCCAACAGCAACTCATGACCGGTTCGAcag       <  1:97594/251‑1 (MQ=255)                                                                                                                                                                                                                             cTGCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCGGTAATTTCCACCAATCCGGCTTAATACCTAATTGATAGAAGTGTGCGAGAATGTCCGCGTAATATTGTTCGTTTTGTTCCATATCCGCCGGCAAAATCACTTCCAACAGCAACTCATGACCGGTTCGAcagcag    <  1:20972/251‑1 (MQ=255)                                                                                                                                                                                                                               gCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTcc                                                                                                                                                                <  1:872034/93‑1 (MQ=255)                                                                                                                                                                                                                               gCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCGGTAATTTCCACCAATCCGGCTTAATACCTAATTGATAGAAGTGTGCGAGAATGTCCGCGTAATATTGTTCGTTTTGTTCCatat                                                      <  1:418719/199‑1 (MQ=255)                                                                                                                                                                                                                               gCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCGGTAATTTCCACCAATCCGGCTTAATACCTAATTGATAGAAGTGTGCGAGAATGTCCGCGTAATATTGTTCGTTTTGTTCCATATCCGCCGGCAAAATCACTTCCAACAGCAACTCATGAc                  >  1:407075/1‑235 (MQ=255)                                                                                                                                                                                                                               gCTTTAAACTGCACTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCGGTAATTTCCACCAATCCGGCTTAATACCTAATTGATAGAAGTGTGCGAGAATGTCCGCGTAATATTGTTCGTTTTGTTCCATATCCGCCGGCAAAATCACTTCCAACAGCAACTCATGACCGGTTCGACAGCAgg   >  1:305905/1‑250 (MQ=255)                                                                                                                                                                                                                                cTTTAACCTGCACTCCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCGGTAATTTCCACCAATCCGGCTTAATACCTAATTGATAGAAGTGTGCGAGAATGTCCGCGTAATATTGTTCGTTTTGTTCCATACCCGCCGGCAAAATCACTTCCAACAGCAACTCATGACCGGTTCGACAGCAGGc  <  1:186644/250‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                               CAAGCAAGTAACAAAAATGCCCCGAACGGGGCATTTTACGTTGATGGTTATTAACGGGATTTCCAAAGTTTAATCAGTCTTCTGTATCTTTCACCCACTTCGGCGATCAGTTCGTCATCTGTTAATTTTCCGGCAAACCAATCCGCCGACGGCTGCCCGAAAATTGTCCGCCCCACTGCAAAGCCTTTTACTAAAGGCGTATTGGCAGACGCATCAAATACTGCTTTAAACTGCGCTTCCGGCGCATCCAAGCCGAGAATCAAAATGCCGCGACAATATGGATCATTTGCCTGAATTACTTCGCTGATACGTTCCCAATCCTGCGCTGAAACGCCCGGTAATTTCCACCAATCCGGCTTAATACCTAATTGATAGAAGTGTGCGAGAATGTCCGCGTAATATTGTTCGTTTTGTTCCATATCCGCCGGCAAAATCACTTCCAACAGCAACTCATGACCGGTTCGACAGCAGGC  >  1/1810935‑1811407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑