Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,808,260	T→C	intergenic (+26360/+27124)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,808,260	0	T	C	100.0%	226.1 / NA	80	intergenic (+26360/+27124)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/44);  total (36/44)

AAAAAGCTGAGAACAGACATATAGCCTCCTTTCTACGCTAGCGTATTTCGTGTGAATTTTTGATGTGATTGAGTTCTGTAAACAGCGCTTCGGTACAGCAACCTAAAATATAATTTCCTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGTTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAGTATCGGGGTGAACACGGTGGGAAACGCCTAATCCCATTAATCCTTCTTTTAAAGAGGTGGAAGAGGAAGCGTGAATAGGTTGATCATTCAGAAATGCGCCATCCCCTTTCATTGCTCTGAACAACTC  >  1/1808043‑1808502                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                   aaaaaGCTGAGAACAGACATATAGCCTCCTTTCTACGCTAGCGTATTTCGTGTGAATTTTTGATGTGATTGAGTTCTGTAAACAGCGCTTCGGTACAGCAACCTAAAATATAATTTCCTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCcaca                                                                                                                                                                                                                   >  1:311186/1‑251 (MQ=255)      gCTGAGAACAGACATATAGCCTCCTTTCTACGCTAGCGTATTTCGTGTGAATTTTTGATGTGATTGAGTTCTGTAAACAGCGCTTCGGTACAGCAACCTAAAATATAATTTCCTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATaaa                                                                                                                                                                                                               >  1:94256/1‑250 (MQ=255)           gaACAGACATATAGCCTCCTTTCTACGCTAGCGTATTTCGTGAGAATTTTTGATGTGATTGAGTTCTGTAAACAGCGCTTCGGTACAGCAACCTAAAATATAATTTCCTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACa                                                                                                                                                                                                         >  1:148860/1‑251 (MQ=255)            aaCAGACATATAGCCTCCTTTCTACGCTAGCGTATTTCGTGTGAATTTTTGATGTGATTGAGTTCTGTAAACAGCGCTTCGGTACAGCAACCTAAAATATAATTTCCTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAAc                                                                                                                                                                                                          <  1:749428/249‑1 (MQ=255)                gACATATAGCCTCCTTTCTACGCTAGCGTATTTCGTGTGAATTTTTGATGTGATTGAGTTCTGTAAACAGCGCTTCGGTACAGCAACCTAAAATATAATTTCCTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGa                                                                                                                                                                                                                               >  1:356316/1‑224 (MQ=255)                gACATATAGCCTCCTTTCTACGCTAGCGTATTTCGTGTGAATTTTTGATGTGATTGAGTTCTGTAAACAGCGCTTCGGTACAGCAACCTAAAATATAATTTCCTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTaa                                                                                                                                                                                                     >  1:197707/1‑250 (MQ=255)                  cATATAGCCTCCTTTCTACGCTAGCGTATTTCGTGTGAATTTTTGATGTGATTGAGTTCTGTAAACAGCGCTTCGGTACAGCAACCTAAAATATAATTTCCTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATaa                                                                                                                                                                                                                <  1:704114/237‑1 (MQ=255)                  cATATAGCCTCCTTTCTACGCTAGCGTATTTCGTGTGAATTTTTGATGTGATTGAGTTCTGTAAACAGCGCTTCGGTACAGCAACCTAAAATATAATTTCCTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACg                                                                                                                                                                                                   >  1:118471/1‑250 (MQ=255)                  cATATAGCCTCCTTTCTACGCTAGCGTATTTCGTGTGAATTTTTGATGTGATTGAGTTCTGTAAACAGCGCTTCGGTACAGCAACCTAAAATATAATTTCCTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGc                                                                                                                                                                                                  <  1:166085/251‑1 (MQ=255)                   atatAGCCTCCTTTCTACGCTAGCGTATTTCGTGTGAATTTTTGATGTGATTGAGTTCTGTAAACAGCGCTTCGGTACAGCAACCTAAAATATAATTTCCTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCa                                                                                                                                                                                                 >  1:527810/1‑251 (MQ=255)                            cctTTCTACGCTAGCGTATTTCGTGTGAATTTTTGATGTGATTGAGTTCTGTAAACAGCGCTTCGGTACAGCAACCTAAAATATAATTTCCTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCg                                                                                                                                                                                        >  1:194185/1‑251 (MQ=255)                            cctTTCTACGCTAGCGTATTTCGTGTGAATTTTTGATGTGATTGAGTTCTGTAAACAGCGCTTCGGTACAGCAACCTAAAATATAATTTCCTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCg                                                                                                                                                                                        >  1:359635/1‑251 (MQ=255)                            cctTTCTACGCTAGCGTATTTCGTGTGAATTTTTGATGTGATTGAGTTCTGTAAACAGCGCTTCGGTACAGCAACCTAAAATATAATTTCCTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCg                                                                                                                                                                                        >  1:304504/1‑251 (MQ=255)                                   aCGCTAGCGTATTTCGTGTGAATTTTTGATGTGATTGAGTTCTGTAAACAGCGCTTCGGTACAGCAACCTAAAATATAATTTCCTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGa                                                                                                                                                                                            >  1:608746/1‑240 (MQ=255)                                   aCGCTAGCGTATTTCGTGTGAATTTTTGATGTGATTGAGTTCTGTAAACAGCGCTTCGGTACAGCAACCTAAAATATAATTTCCTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGaa                                                                                                                                                                                 <  1:108274/251‑1 (MQ=255)                                   aCGCTAGCGTATTTCGTGTGAATTTTTGATGTGATTGAGTTCTGTAAACAGCGCTTCGGTACAGCAACCTAAAATATAATTTCCTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGaa                                                                                                                                                                                 >  1:654090/1‑251 (MQ=255)                                        aGCGTATTTCGTGTGAATTTTTGATGTGATTGAGTTCTGTAAACAGCGCTTCGGTACAGCAACCTAAAATATAATTTCCTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAAc                                                                                                                                                                                          >  1:857141/1‑237 (MQ=255)                                        aGCGTATTTCGTGTGAATTTTTGATGTGATTGAGTTCTGTAAACAGCGCTTCGGTACAGCAACCTAAAATATAATTTCCTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGaa                                                                                                                                                                             >  1:851980/1‑250 (MQ=255)                                        aGCGTATTTCGTGTGAATTTTTGATGTGATTGAGTTCTGTAAACAGCGCTTCGGTACAGCAACCTAAAATATAATTTCCTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGaa                                                                                                                                                                             >  1:363347/1‑250 (MQ=255)                                          cGTATTTCGTGTGAATTTTTGATGTGATTGAGTTCTGTAAACAGCGCTTCGGTACAGCAACCTAAAATATAATTTCCTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAAc                                                                                                                                                                            <  1:290478/249‑1 (MQ=255)                                               ttCGTGTGAATTTTTGATGTGATTGAGTTCTGTAAACAGCGCTTCGGTACAGCAACCTAAAATATAATTTCCTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCAc                                                                                                                                                                     <  1:192833/251‑1 (MQ=255)                                                               aTGTGATTGAGTTCTGTAAACAGCGCTTCGGTACAGCAACCTAAAATATAATTTCCTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAAttt                                                                                                                                                      <  1:403241/250‑1 (MQ=255)                                                                  tgATTGAGTTCTGTAAACAGCGCTTCGGTACAGCAACCTAAAATATAATTTCCTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGt                                                                                                                                                                  >  1:217706/1‑235 (MQ=255)                                                                    aTTGAGTTCTGTAAACAGCGCTTCGGTACAGCAACCTAAAATATAATTTCCTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGc                                                                                                                                                                                                  <  1:889006/201‑1 (MQ=255)                                                                         gTTCTGTAAACAGCGCTTCGGTACAGCAACCTAAAATATAATTTCCTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAAc                                                                                                                                                                                          >  1:286795/1‑204 (MQ=255)                                                                             tGTAAACAGCGCTTCGGTACAGCAACCTAAAATATAATTTCCTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAg                                                                                                                                       <  1:66992/251‑1 (MQ=255)                                                                             tGTAAACAGCGCTTCGGTACAGCAACCTAAAATATAATTTCCTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAg                                                                                                                                       <  1:245202/251‑1 (MQ=255)                                                                                aaaCAGCGCTTCGGTACAGCAACCTAAAATATAATTTCCTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGACTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTaa                                                                                                                                    >  1:621491/1‑251 (MQ=255)                                                                                   caccgcTTCGGTACAGCAACCTAAAATATAATTTCCTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAg                                                                                                                                 <  1:166940/248‑1 (MQ=255)                                                                                      cgcTTCGGTACAGCAACCTAAAATATAATTTCCTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAg                                                                                                                                 <  1:296059/248‑1 (MQ=255)                                                                                      cgcTTCGGTACAGCAACCTAAAATATAATTTCCTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAg                                                                                                                                 <  1:832261/248‑1 (MQ=255)                                                                                      cgcTTCGGTACAGCAACCTAAAATATAATTTCCTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACAGAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAg                                                                                                                                 <  1:894806/248‑1 (MQ=255)                                                                                           cGGTACAGCAACCTAAAATATAATTTCCTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAGTATCgggg                                                                                                                         <  1:837537/251‑1 (MQ=255)                                                                                                      ccTAAAATATAATTTCCTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTCATATGCGGCTCAAAATCGCCAATCAAGCGACCTGCCGCCACATACACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAGTATCGGGGTGAACACGGTg                                                                                                              >  1:387292/1‑251 (MQ=255)                                                                                                      ccTAAAATATAATTTCCTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAGTATCGGGGTGAACACGGTg                                                                                                              >  1:812097/1‑251 (MQ=255)                                                                                                           aaTATAATTTCCTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAGTATCGGGGTGAACACGGTGGGaaa                                                                                                         <  1:872427/251‑1 (MQ=255)                                                                                                           aaTATAATTTCCTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAGTATCGGGGTGAACACGGTGGGaaa                                                                                                         <  1:42595/251‑1 (MQ=255)                                                                                                            atatAATTTCCTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAGTATCGGGGTGAACACGGTGGGAAAc                                                                                                        <  1:261784/251‑1 (MQ=255)                                                                                                              ataATTTCCTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAGTATCGGGGTGAACACGGTGGGAAACg                                                                                                       >  1:316035/1‑250 (MQ=255)                                                                                                                   ttCCTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAGTATCGGGGTGAACACGGTGGGatgttcattcg                                                                                                 <  1:239057/251‑11 (MQ=255)                                                                                                                     ccTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGcac                                                                                                                                            <  1:826348/206‑1 (MQ=255)                                                                                                                     ccTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAg                                                                                                                                 <  1:489418/217‑1 (MQ=255)                                                                                                                     ccTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAGTATCGGGGTGAACACGGTGGGAAACGCCTAATcc                                                                                               <  1:634581/251‑1 (MQ=255)                                                                                                                               gTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAg                                                                                                                                       >  1:901126/1‑201 (MQ=255)                                                                                                                               gTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAGTATCGGGGTGAACACGGTGGGAAACGCCTAATCCCATTAATcc                                                                                      >  1:853163/1‑250 (MQ=255)                                                                                                                                       ttttCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAGTATCGGGGTGAACACGGTGGGAAACGCCTAATCCCATTAATCCTTCTTTTaa                                                                             >  1:141575/1‑251 (MQ=255)                                                                                                                                         ttCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAGTATCGGGGTGAACACGGTGGGAAACGCCTAATCCCATTAATCCTTCTTTTAAag                                                                           <  1:16596/251‑1 (MQ=255)                                                                                                                                               gAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAGTATCGGGGTGAACACGGTGGGAAACGCCTAATcc                                                                                               <  1:747784/225‑1 (MQ=255)                                                                                                                                                aaaTTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAAt                                                                                                                                                                                                                                             >  1:42023/1‑82 (MQ=255)                                                                                                                                                aaaTTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAAc                                                                                                                                                                            <  1:148768/147‑1 (MQ=255)                                                                                                                                                aaaTTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAg                                                                                                                                 <  1:196459/190‑1 (MQ=255)                                                                                                                                                       tCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAGTATCGGGGTGAACACGGTGGGAAACGCCTAATCCCATTAATCCTTCTTTTAAAGAGGTGGAAGAGGaa                                                             >  1:343504/1‑251 (MQ=255)                                                                                                                                                          caTGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACAtt                                                                                                                                                                                                       <  1:243494/110‑1 (MQ=255)                                                                                                                                                          caTGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAg                                                                                                                                       >  1:58566/1‑174 (MQ=255)                                                                                                                                                                cgcccgcTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAGTATCGGGGTGAACACGGTGGGAAACGCCTAATCCCATTAATCCTTCTTTTAAAGAGGTGGAAGAGGAAGCGTGAATa                                                    <  1:482153/251‑1 (MQ=255)                                                                                                                                                                   ccgcTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGTCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAGTATCGGGGTGAACACGGTGGGAAACGCCTAATCCCATTAATCCTTCTTTTAAAGAGGTGGAAGAGGAAGCGTGAATAGGt                                                 <  1:435979/251‑1 (MQ=255)                                                                                                                                                                      cTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAGTATCGGGGTGAACACGGTGGGAAACGCCTAATCCCATTAATCCTTCTTTTAAAGAGGTGGAagag                                                                <  1:302480/233‑1 (MQ=255)                                                                                                                                                                         ccTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAGTATCGGGGTGAACACGGTGGGAAACGCCTAATCCCATTAATCCTTCTTTTAAAGAGGTGGAAGAGGAAGCGTGAATAGGTTGATCa                                           <  1:762172/251‑1 (MQ=255)                                                                                                                                                                            gAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCg                                                                                                                                                                                        >  1:598676/1‑107 (MQ=255)                                                                                                                                                                            gAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTaaaa                                                                                                                                  <  1:683361/161‑1 (MQ=255)                                                                                                                                                                               cAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAGTATCGGGGTGAACACGGt                                                                                                               >  1:356873/1‑177 (MQ=255)                                                                                                                                                                                 ataatGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGaa                                                                                                                                                                             <  1:631686/113‑1 (MQ=255)                                                                                                                                                                                 ataatGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAGTATCGGGGTGAACACGGTGGGAAACGCCTAATCCCATTAATCCTTCTTTTAAAGAGGTGGAAGAGGAAGCGTGAATAGGTTGATCATTCAGaaa                                   >  1:692364/1‑251 (MQ=255)                                                                                                                                                                                 ataatGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAGTATCGGGGTGAACACGGTGGGAAACGCCTAATCCCATTAATCCTTCTTTTAAAGAGGTGGAAGAGGAAGCGTGAATAGGTTGATCATTCAGaaa                                   <  1:721729/251‑1 (MQ=255)                                                                                                                                                                                 ataatGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAGTATCGGGGTGAACACGGTGGGAAACGCCTAATCCCATTAATCCTTCTTTTAAAGAGGTGGAAGAGGAAGCGTGAATAGGTTGATCATTCAGaaa                                   <  1:580456/251‑1 (MQ=255)                                                                                                                                                                                 ataatGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAGTATCGGGGTGAACACGGTGGGAAACGCCTAATCCCATTAATCCTTCTTTTAAAGAGGTGGAAGAGGAAGCGTGAATAGGTTGATCATTCAGaaa                                   <  1:327507/251‑1 (MQ=255)                                                                                                                                                                                 ataatGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAGTATCGGGGTGAACACGGTGGGAAACGCCTAATCCCATTAATCCTTCTTTTAAAGAGGTGGAAGAGGAAGCGTGAATAGGTTGATCATTCAGaaa                                   >  1:260663/1‑251 (MQ=255)                                                                                                                                                                                 ataatGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAGTATCGGGGTGAACACGGTGGGAAACGCCTAATCCCATTAATCCTTCTTTTAAAGAGGTGGAAGAGGAAGCGTGAATAGGTTGATCATTCAGaaa                                   >  1:41352/1‑251 (MQ=255)                                                                                                                                                                                                cAAACATCCCAAGCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAg                                                                                                                                 >  1:320251/1‑142 (MQ=255)                                                                                                                                                                                                            gCGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAGTATCGGGGTGAACACGGTGGGAAACGCCTAATCCCATTAATCCTTCTTTTAAAGAGGTGGAAGAGGAAGCGTGAATAGGTTGATCATTCAGAAATGCGCCATCCCCTTTCATTGATCTGaa        >  1:261321/1‑251 (MQ=255)                                                                                                                                                                                                             cGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAGTATCGGGGTGAACACGGTGGGAAACGCCTAATCCCATTAATCCTTCTTTTAAAGAGGTGGAAGAGGAAGCGTGAATAGGTTGATCATTCAGAAATGCGCCATCCCCTTTCATTGCTCTGaac       <  1:160106/251‑1 (MQ=255)                                                                                                                                                                                                             cGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAGTATCGGGGTGAACACGGTGGGAAACGCCTAATCCCATTAATCCTTCTTTTAAAGAGGTGGAAGAGGAAGCGTGAATAGGTTGATCATTCAGAAATGCGCCATCCCCTTTCATTGCTCTGaac       <  1:889125/251‑1 (MQ=255)                                                                                                                                                                                                             cGTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAGTATCGGGGTGAACACGGTGGGAAACGCCTAATCCCATTAATCCTTCTTTTAAAGAGGTGGAAGAGGAAGCGTGAATAGGTTGATCATTCAGAAATGCGCCATCCCCTTTCATTGCTCTGaac       <  1:377156/251‑1 (MQ=255)                                                                                                                                                                                                              gTTAATATGCGGCTCAAAATAGCCAATCAAGCGGCCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCg                                                                                                                                                                   <  1:882047/94‑1 (MQ=255)                                                                                                                                                                                                              gTTAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAGTATCGGGGTGAACACGGTGGGAAACGCCTAATCCCATTAATCCTTCTTTTAAAGAGGTGGAAGAGGAAGCGTGAATAGGTTGATCATTCAGAAATGCGCCATCCCCTTTCATTGCTCTGaaca      >  1:309798/1‑251 (MQ=255)                                                                                                                                                                                                               ttAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAGTATCGGGGTGAACACGGTGGGAAACGCCTAATCCCATTAATCCTTCTTTTAAAGAGGTGGAAGAGGAAGCGTGAAt                                                     >  1:513033/1‑203 (MQ=255)                                                                                                                                                                                                                tAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAGTATCGGGGTGAACACGGTGGGAAACGCCTAATCCCATTAATCCTTCTTTTAAAGAGGTGGAAGAGGAAGCGTGAATAGGTTGATCATTCAGAAATGCGCCATCCCCTTTCATTGCTCTGaacaac    <  1:46857/251‑1 (MQ=255)                                                                                                                                                                                                                tAATATGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAGTATCGGGGTGAACACGGTGGGAAACGCCTAATCCCATTAATCCTTCTTTTAAAGAGGTGGAAGAGGAAGCGTGAATAGGTTGATCATTCAGAAATGCGCCATCCCCTTTCATTGCTCTGaacaac    <  1:800344/251‑1 (MQ=255)                                                                                                                                                                                                                  atatGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACTAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAGTATCGGGGTGAACACGGTGGGAAACGCCTAATCCCATTAATCCTTCTTTTAAAGAGGTGGAAGAGGAAGCGTGAATAGGTTGATCATTCAGAAATGCGCCATCCCCTTTCATTGCTCTGAACAACTc  <  1:515191/251‑1 (MQ=255)                                                                                                                                                                                                                  atatGCGGCTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAGTATCGGGGTGAACACGGTGGGAAACGCCTAATCCCATTAATCCTTCTTTTAAAGAGGTGGAAGAGGAAGCGTGAATAGGTTGATCATTCAGAAATGCGCCATCCCCTTTCATTGCTCTGAACAACTc  <  1:713079/251‑1 (MQ=255)                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                   AAAAAGCTGAGAACAGACATATAGCCTCCTTTCTACGCTAGCGTATTTCGTGTGAATTTTTGATGTGATTGAGTTCTGTAAACAGCGCTTCGGTACAGCAACCTAAAATATAATTTCCTTTGCCGAGTCCTTCATTTTCAAGGAAATTATTCACATGCCCGCCCGCTTCCTGAACAATAATGATCGCGGCGCAAACATCCCAAGCGTTAATATGCGGTTCAAAATAGCCAATCAAGCGACCTGCCGCCACATAAACCAACATTAACGCACCGGAACCGTTACGAATGAACATCCCACCGTCCAATAAAATTTGGTGTAGCACAGGAGTAAAAGTATCGGGGTGAACACGGTGGGAAACGCCTAATCCCATTAATCCTTCTTTTAAAGAGGTGGAAGAGGAAGCGTGAATAGGTTGATCATTCAGAAATGCGCCATCCCCTTTCATTGCTCTGAACAACTC  >  1/1808043‑1808502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑