Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,801,177	A→C	intergenic (+19277/+34207)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,801,177	0	A	C	100.0%	217.3 / NA	72	intergenic (+19277/+34207)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (31/41);  total (31/41)

AACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCGGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATAAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGCACTTTCATCAAGGTGGATTTCCCGGCACCGTTTTCCCCCATTAAGGCATGAACGCTTCCACGTTTTACGCGAAGATTAATGCCGTCAAGTGCCTTCACCCCCGGAAATGTTTTTGACACATTTCGCATTTCTAAAATATACGGAGAATTTGAAC  >  1/1800932‑1801410                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                          aaCCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGTAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAg                                                                                                                                                                                                                                      <  1:492298/251‑1 (MQ=255)              atagcaaCTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGttt                                                                                                                                                                                                                         <  1:404180/251‑1 (MQ=255)                              cTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTc                                                                                                                                                                                                          >  1:641460/1‑250 (MQ=255)                                    ccACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAaat                                                                                                                                                                                                    <  1:744952/250‑1 (MQ=255)                                       cagATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAg                                                                                                                                                                                                                     >  1:34955/3‑230 (MQ=255)                                             cATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCg                                                                                                                                                                                          >  1:877103/1‑251 (MQ=255)                                              aTCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGCCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGc                                                                                                                                                                                         <  1:71263/251‑1 (MQ=255)                                                cGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCca                                                                                                                                                                                       <  1:132475/251‑1 (MQ=255)                                                 ggACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCcat                                                                                                                                                                                      >  1:168849/1‑251 (MQ=255)                                                            cagTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGat                                                                                                                                                                            <  1:730231/248‑1 (MQ=255)                                                               tCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAg                                                                                                                                                                        <  1:183038/251‑1 (MQ=255)                                                               tCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAg                                                                                                                                                                        <  1:86193/251‑1 (MQ=255)                                                                cACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGa                                                                                                                                                                       <  1:63625/251‑1 (MQ=255)                                                                cACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGa                                                                                                                                                                       <  1:264343/251‑1 (MQ=255)                                                                cACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGa                                                                                                                                                                       <  1:513271/251‑1 (MQ=255)                                                                cACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGa                                                                                                                                                                       <  1:806901/251‑1 (MQ=255)                                                                cACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGa                                                                                                                                                                       <  1:372300/251‑1 (MQ=255)                                                                   gTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAg                                                                                                                                                                                                                                      <  1:557342/185‑1 (MQ=255)                                                                                    gCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGc                                                                                                                                                            >  1:733530/1‑242 (MQ=255)                                                                                       ctTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAg                                                                                                                                                                                                                     <  1:122450/182‑1 (MQ=255)                                                                                       ctTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAaat                                                                                                                                                                                                    >  1:528619/1‑199 (MQ=255)                                                                                       ctTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATtc                                                                                                                                                                                              >  1:137715/1‑205 (MQ=255)                                                                                       ctTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGa                                                                                                                                                                       >  1:469093/1‑228 (MQ=255)                                                                                       ctTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGCACTTtca                                                                                                                                                     <  1:286594/246‑1 (MQ=255)                                                                                       ctTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGCACTTTCATCAAg                                                                                                                                                >  1:186701/1‑251 (MQ=255)                                                                                       ctTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGCACTTTCATCAAg                                                                                                                                                >  1:653113/1‑251 (MQ=255)                                                                                                 cGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAGAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGa                                                                                                                                                                       >  1:451414/1‑218 (MQ=255)                                                                                                      aaGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGCACTTTCATCAAGGTGGATTTCCCGGc                                                                                                                                  <  1:419275/250‑1 (MQ=255)                                                                                                         ccTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGCACTTTCATCAAGGTGGATTTCCCGGCAc                                                                                                                                <  1:860272/249‑1 (MQ=255)                                                                                                            gCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGCACTTTCATCAAGGTGGATTTCCCGGCACCGttt                                                                                                                           >  1:768351/1‑251 (MQ=255)                                                                                                               cATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGCACTTTCATCAAGGTGGATTTCCCGGCACCGTTTTc                                                                                                                         >  1:156268/1‑250 (MQ=255)                                                                                                                         tttCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGCACTTTCATCAAGGTGGATTTCCCGGCACCGTTTTCCCCCATTAAgg                                                                                                              <  1:584457/251‑1 (MQ=255)                                                                                                                          ttCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGCACTTTCATCAAGGTGGATTTCCCGGCACCGTTTTCCCCCATTAAGGc                                                                                                             <  1:335002/251‑1 (MQ=255)                                                                                                                          ttCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGCACTTTCATCAAGGTGGATTTCCCGGCACCGTTTTCCCCCATTAAGGc                                                                                                             <  1:856049/251‑1 (MQ=255)                                                                                                                          ttCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGCACTTTCATCAAGGTGGATTTCCCGGCACCGTTTTCCCCCATTAAGGc                                                                                                             <  1:442010/251‑1 (MQ=255)                                                                                                                                tCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGCACTTTCATCAAGGTGGATTTCCCGGCACCGTTTTCCCCCATTAAGGCATGAAc                                                                                                       <  1:56878/251‑1 (MQ=255)                                                                                                                                   agaAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGCACTTTCATCAAGGTGGATTTCCCGGCACCGTTTTCCCCCATTAAGGCATGAACg                                                                                                      >  1:803728/1‑249 (MQ=255)                                                                                                                                   agaAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGCACTTTCATCAAGGTGGATTTCCCGGCACCGTTTTCCCCCATTAAGGCATGAACg                                                                                                      >  1:684016/1‑249 (MQ=255)                                                                                                                                    gaAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCGTCCGGGATATAGATGCCATACAGCACTTTCATCAAGGTGGATTTCCCGGCACCGTTTTCCCCCATTAAGGCATGAACGCtt                                                                                                   >  1:453418/1‑251 (MQ=255)                                                                                                                                    gaAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGc                                                                                                                                                                    <  1:80089/186‑1 (MQ=255)                                                                                                                                    gaAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGCACTTTCATCAAGGTGGATTTCCCGGCACCGTTTTCCCCCATTAAGGCATGAACGCtt                                                                                                   >  1:818865/1‑251 (MQ=255)                                                                                                                                          aCTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGCACTTTCATCAAGGTGGATTTCCCGGCACCGTTTTCCCCCATTAAGGCATGAACGCTTCCACGt                                                                                             <  1:223628/251‑1 (MQ=255)                                                                                                                                              ggcttggGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACACCACTTTCATCAAGGTGGATTTCCCGGCACCGTTTTCCCCCATTAAGGCATGAACGCTTCCACGTTTTa                                                                                         >  1:41502/1‑251 (MQ=255)                                                                                                                                                cttggGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGCACTTTCATCAAGGTGGATTTCCCGGCACCGTTTTCCCCCATTAAGGCATGAACGCTTCCACGTTTTAcg                                                                                       <  1:473586/251‑1 (MQ=255)                                                                                                                                                   gggTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGCACTTTCATCAAGGTGGATTTCCCGGCACCGTTTTCCCCCATTAAGGCATGAACGCTTCCACGTTTTACGCGa                                                                                    >  1:692102/1‑251 (MQ=255)                                                                                                                                                          ttCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGa                                                                                                                                                                       >  1:536239/1‑161 (MQ=255)                                                                                                                                                             aCGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGCACTTTCATCAAGGTGGATTTCCCGGCACCGTTTTCCCCCATTAAGGCATGAACGCTTCCACGTTTTACGCGAAGATTAATGc                                                                          <  1:737130/251‑1 (MQ=255)                                                                                                                                                             aCGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGCACTTTCATCAAGGTGGATTTCCCGGCACCGTTTTCCCCCATTAAGGCATGAACGCTTCCACGTTTTACGCGAAGATTAATGc                                                                          <  1:857682/251‑1 (MQ=255)                                                                                                                                                                cccTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGgac                                                                                                                                                                                                                                   >  1:456688/1‑95 (MQ=255)                                                                                                                                                                 ccTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGCACTTTCATCAAGGTGGATTTCCCGGCACCGTTTTCCCCCATTAAGGCATGAACGCTTCCACGTTTTACGCGAAGATTAATGCCGTc                                                                      <  1:244334/251‑1 (MQ=255)                                                                                                                                                                    aaGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGc                                                                                                                                                                                         <  1:684563/133‑1 (MQ=255)                                                                                                                                                                    aaGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATAc                                                                                                                                                               >  1:728472/1‑159 (MQ=255)                                                                                                                                                                    aaGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGc                                                                                                                                                            <  1:822240/162‑1 (MQ=255)                                                                                                                                                                    aaGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGCACTTTCATCAAgg                                                                                                                                               <  1:119945/175‑1 (MQ=255)                                                                                                                                                                      gTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATAc                                                                                                                                                               >  1:818994/1‑157 (MQ=255)                                                                                                                                                                        aaaaaTTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGCACTTTCATCAAGGTGGATTTCCCGGCACCGTTTTCCCCCATTAAGGCATGAACGCTTCCACGTTTTACGCGAAGATTAATGCCGTCAAGTGcc                                                               <  1:81877/251‑1 (MQ=255)                                                                                                                                                                           aaTTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGCACTTTCATCAAGGTGGATTTCCCGGCACCGTTTTCCCCCATTAAGGCATGAACGCTTCCACGTTTTACGCGAAGATTAATGCCGTCAAGTGCCtt                                                             >  1:246334/1‑250 (MQ=255)                                                                                                                                                                            aTTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGCACTTtca                                                                                                                                                     >  1:143453/1‑161 (MQ=255)                                                                                                                                                                                             aaTTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGCACTTTCATCAAGGTGGATTTCCCGGCACCGTTTTCCCCCATTAAGGCATGAACGCTTCCACGTTTTACGCGAAGATTAATGCCGTCAAGTGCCTTCACCCCCGGAAATGttttt                                          >  1:657064/1‑251 (MQ=255)                                                                                                                                                                                             aaTTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGCACTTTCATCAAGGTGGATTTCCCGGCACCGTTTTCCCCCATTAAGGCATGAACGCTTCCACGTTTTACGCGAAGATTAATGCCGTCAAGTGCCTTCACCCCCGGAAATGttttt                                          >  1:795229/1‑251 (MQ=255)                                                                                                                                                                                                           gAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGCACTTTCATCAAGGTGGATTTCCCGGCACCGTTTTCCCCCATTAAGGCATGAACGCTTCCACGTTTTACGCGAAGATTAATGCCGTCAAGTGCCTTCACCCCCGGAAATGTTTTTGACACATTTCGCa                             <  1:577045/250‑1 (MQ=255)                                                                                                                                                                                                              aTTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGCACTTTCATCAAGGTGGATTTCCCGGCACCGTTTTCCCCCATTAAGGCATGAACGCTTCCACGTTTTACGCGAAGATTAATGCCGTCAAGTGCCTTCACCCCCGGAAATGTTTTTGACACATTTCGCATTTc                         <  1:538125/251‑1 (MQ=255)                                                                                                                                                                                                                      cGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGCACTTTCATCAAGGTGGATTTCCCGGCACCGTTTTCCCCCATTAAGGCATGAACGCTTCCACGTTTTACGCGAAGATTAATGCCGTCAAGTGCCTTCACCCCCGGAAATGTTTTTGACACATTTCGCATTTCTAAAatat                 <  1:270583/251‑1 (MQ=255)                                                                                                                                                                                                                              tGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGCACTTTCATCAAg                                                                                                                                                >  1:73358/1‑116 (MQ=255)                                                                                                                                                                                                                              tGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGCACTTTCATCAAGGTGGATTTCCCGGCACCGTTTTCCCCCATTAAGGCATGAACGCTTCCACGTTTTACGCGAAGATTAATGCCGTCAAGTGCCTTCACCCCCGGAAATGTTTTTGACACATTTCGCATTTCTAAAATATACGGAGaa         >  1:213518/1‑251 (MQ=255)                                                                                                                                                                                                                                 ttAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGCACTTTCATCAAGGTGGATTTCCCGGCACCGTTTTCCCCCATTAAGGCATGAACGCTTCCACGTTTTACGCGAAGATTAATGCCGTCAAGTGCCTTCACCCCCGGAAATGTTTTTGACACATTTCGCATTTCTAAAATATACGGAGAAtt       <  1:820656/250‑1 (MQ=255)                                                                                                                                                                                                                                  tAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGCACTTTCATCAAGGTGGATTTCCCGGCACCGTTTTCCCCCATTAAGGCATGAACGCTTCCACGTTTTACGCGAAGATTAATGCCGTCAAGTGCCTTCACCCCCGGAAATGTTTTTGACACATTTCGCATTTCTAAAATATACGGAGAATTTg     <  1:50557/251‑1 (MQ=255)                                                                                                                                                                                                                                    aGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGCACTTTCATCAAGGTGGATTTCCCGGCACCGTTTTCCCCCATTAAGGCATGAACGCTTCCACGTTTTACGCGAAGATTAATGCCGTCAAGTGCCTTCACCCCCGGAAATGTTTTTGACACATTTCGCATTTCTAAAATATACGGAGAATTTGaa   >  1:365369/1‑251 (MQ=255)                                                                                                                                                                                                                                      ccGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGCACTTTCATCAAGGTGGATTTCCCGGCACCGTTTTCCCCCATTAAGGCATGAACGCTTCCACGTTTTACGCGAAGATTAATGCCGTCAAGTGCCTTCACCCCCGGAAATGTTTTTGACACATTTCGCATTTCTAAAATATACGGAGAATTTGAAc  <  1:301980/250‑1 (MQ=255)                                                                                                                                                                                                                                         cAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGCACTTTCATCAAGGTGGATTTCCCGGCACCGTTTTCCCCCATTAAGGCATGAACGCTTCCACGt                                                                                             <  1:612687/156‑1 (MQ=255)                                                                                                                                                                                                                                         cAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGCACTTTCATCAAGGTGGATTTCCCGGCACCGTTTTCCCCCATTAAGGCATGAACGCTTCCACGTTTTACGCGAAGATTAATGCCGTCAAGTGCCTTCAc                                                          <  1:528185/191‑1 (MQ=255)                                                                                                                                                                                                                                             gAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGCACTTTCATCAAGGTGGATTTCCCGGCACCGTTTTCCCCCATTAAGGCATGAACGCTTCCACGTTTTACGCGAAGATTAATGCCGTCAAGTGCCTTCACCCCCGGAAATGTTTTTGACACATTTCGCATTTCTaaaa                    >  1:504591/1‑225 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                          AACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCGGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATAAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCATCATCCGGGATATAGATGCCATACAGCACTTTCATCAAGGTGGATTTCCCGGCACCGTTTTCCCCCATTAAGGCATGAACGCTTCCACGTTTTACGCGAAGATTAATGCCGTCAAGTGCCTTCACCCCCGGAAATGTTTTTGACACATTTCGCATTTCTAAAATATACGGAGAATTTGAAC  >  1/1800932‑1801410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑