Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,793,838	A→G	intergenic (+11938/+41546)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,793,838	0	A	G	100.0%	173.8 / NA	62	intergenic (+11938/+41546)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/30);  total (32/30)

CTTCAGTCTATCCCTTCGTGGCAAGGCTATGCAGGTTCAAGTACCCGAACATATCTGGATCCTGATCTTATCTCGCGCGTAGAAATAGAAAAAGGCCCGTCACTCAAAGCAGATGCTACCGGCGCCACAGGTGGTGTAGTGAGAATGAATACCGTAGGCTACAAAGACATAATCCCCGAAAATAAGAACTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCACCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCAAAAATCCTTTTCAGAATTTAGGCAAAAGCTACAACTACTCGCTCGCATTTTCTAAAAAGTGGGAAAACGCCGACATTGTGTTGGCCTACGCCAAAAAGAAGCAGGGCAATTATTTCGTCGGACGCCATGGCGC  >  1/1793599‑1794083                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                      cTTCAGTCTATCCCTTCGTGGCAAGGCTATGCAGGTTCAAGTACCCGAACATATCTGGATCCTGATCTTATCTCGCGCGTAGAAATAGAAAAAGGCCCGTCACTCAAAGCAGATGCTACCGGCGCCACAGGTGGTGTAGTGAGAATGAATACCGTAGGCTACAAAGACATAATCCCCGAAAATAAGAACTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTtata                                                                                                                                                                                                                                             >  1:14579/1‑250 (MQ=255) cTTCAGTCTATCCCTTCGTGGCAAGGCTATGCAGGTTCAAGTACCCGAACATATCTGGATCCTGATCTTATCTCGCGCGTAGAAATAGAAAAAGGCCCGTCACTCAAAGCAGATGCTACCGGCGCCACAGGTGGTGTAGTGAGAATGAATACCGTAGGCTACAAAGACATAATCCCCGAAAATAAGAACTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATac                                                                                                                                                                                                                                            <  1:815837/251‑1 (MQ=255)               ttCGTGGCAAGGCTATGCAGGTTCAAGTACCCGAACATATCTGGATCCTGATCTTATCTCGCGCGTAGAAATAGAAAAAGGCCCGTCACTCAAAGCAGATGCTACCGGCGCCACAGGTGGTGTAGTGAGAATGAATACCGTAGGCTACAAAGACATAATCCCCGAAAATAAGAACTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATAcc                                                                                                                                                                                                                              <  1:749070/251‑1 (MQ=255)                   tGGCAAGGCTATGCAGGTTCAAGTACCCGAACATATCTGGATCCTGATCTTATCTCGCGCGTAGAAATAGAAAAAGGCCCGTCACTCAAAGCAGATGCTACCGGCGCCACAGGTGGTGTAGTGAGAATGAATACCGTAGGCTACAAAGACATAATCCCCGAAAATAAGAACTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTAATACACGCGGCGGATACCGCa                                                                                                                                                                                                                           >  1:80673/1‑250 (MQ=255)                                        aGTACCCGAACATATCTGGATCCTGATCTTATCTCGCGCGTAGAAATAGAAAAAGGCCCGTCACTCAAAGCAGATGCTACCGGCGCCACAGGTGGTGTAGTGAGAATGAATACCGTAGGCTACAAAGACATAATCCCCGAAAATAAGAACTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGAt                                                                                                                                                                                                                 <  1:179196/239‑1 (MQ=255)                                         gTACCCGAACATATCTGGATCCTGATCTTATCTCGCGCGTAGAAATAGAAAAAGGCCCGTCACTCAAAGCAGATGCTACCGGCGCCACAGGTGGTGTAGTGAGAATGAATACCGTAGGCTACAAAGACATAATCCCCGAAAATAAGAACTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGt                                                                                                                                                                                                    >  1:167349/1‑251 (MQ=255)                                          tACCCGAACATATCTGGATCCTGATCTTATCTCGCGCGTAGAAATAGAAAAAGGCCCGTCACTCAAAGCAGATGCTACCGGCGCCACAGGTGGTGTAGTGAGAATGAATACCGTAGGCTACAAAGACATAATCCCCGAAAATAAGAACTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTa                                                                                                                                                                                                   >  1:4024/1‑251 (MQ=255)                                                             ccTGATCTTATCTCGCGCGTAGAAATAGAAAAAGGCCCGTCACTCAAAGCAGATGCTACCGGCGCCACAGGTGGTGTAGTGAGAATGAATACCGTAGGCTACAAAGACATAATCCCCGAAAATAAGAACTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATg                                                                                                                                                                                <  1:493147/251‑1 (MQ=255)                                                                gATCTTATCTCGCGCGTAGAAATAGAAAAAGGCCCGTCACTCAAAGCAGATGCTACCGGCGCCACAGGTGGTGTAGTGAGAATGAATACCGTAGGCTACAAAGACATAATCCCCGAAAATAAGAACTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGt                                                                                                                                                                                                       >  1:406559/1‑225 (MQ=255)                                                                gATCTTATCTCGCGCGTAGAAATAGAAAAAGGCCCGTCACTCAAAGCAGATGCTACCGGCGCCACAGGTGGTGTAGTGAGAATGAATACCGTAGGCTACAAAGACATAATCCCCGAAAATAAGAACTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTc                                                                                                                                                                              >  1:299520/1‑250 (MQ=255)                                                                 aTCTTATCTCGCGCGTAGAAATAGAAAAAGTCCCGTCACTCAAAGCAGATGCTACCGGCGCCACAGGTGGTGTAGTGAGAATGAATACCGTAGGCTACAAAGACATAATCCCCGAAAATAAGAACTGGGGACTTCGCTTGCGGTTCGGTACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATAc                                                                                                                                                                                                                               >  1:780034/1‑200 (MQ=255)                                                                 aTCTTATCTCGCGCGTAGAAATAGAAAAAGGCCCGTCACTCAAAGCAGATGCTACCGGCGCCACAGGTGGTGTAGTGAGAATGAATACCGTAGGCTACAAAGACATAATCCCCGAAAATAAGAACTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCAc                                                                                                                                                                                                                          <  1:61358/205‑1 (MQ=255)                                                                          cgcgcgTAGAAATAGAAAAAGGCCCGTCACTCAAAGCAGATGCTACCGGCGCCACAGGTGGTGTAGTGAGAATGAATACCGTAGGCTACAAAGACATAATCCCCGAAAATAAGAACTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAAcc                                                                                                                                                                   <  1:530512/251‑1 (MQ=255)                                                                                  gaaatagaaaAAGGCCCGTCACTCAAAGCAGATGCTACCGGCGCCACAGGTGGTGTAGTGAGAATGAATACCGTAGGCTACAAAGACATAATCCCCGAAAATAAGAACTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAAcc                                                                                                                                                            >  1:350936/1‑250 (MQ=255)                                                                                      tagaaaAAGGCCCGTCACTCAAAGCAGATGCTACCGGCGCCACAGGTGGTGTAGTGAGAATGAATACCGTAGGCTACAAAGACATAATCCCCGAAAATAAGAACTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCg                                                                                                                                                                                            <  1:734358/214‑1 (MQ=255)                                                                                               gCCCGTCACTCAAAGCAGATGCTACCGGCGCCACAGGTGGTGTAGTGAGAATGAATACCGTAGGCTACAAAGACATAATCCCCGAAAATAAGAACTGGGGACTTCGCTTGCGGATCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATa                                                                                                                                               >  1:601903/1‑250 (MQ=255)                                                                                                       cTCAAAGCAGATGCTACCGGCGCCACAGGTGGTGTAGTGAGAATGAATACCGTAGGCTACAAAGACATAATCCCCGAAAATAAGAACTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCaa                                                                                                                                      >  1:349976/1‑251 (MQ=255)                                                                                                           aaGCAGATGCTACCGGCGCCACAGGTGGTGTAGTGAGAATGAATACCGTAGGCTACAAAGACATAATCCCCGAAAATAAGAACTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCAAAAAt                                                                                                                                  >  1:133347/1‑251 (MQ=255)                                                                                                             gCAGATGCTACCGGCGCCACAGGTGGTGTAGTGAGAATGAATACCGTAGGCTACAAAGACATAATCCCCGAAAATAAGAACTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCAAAAATc                                                                                                                                 >  1:456076/1‑250 (MQ=255)                                                                                                                                  ggtggtGTAGTGAGAATGAATACCGTAGGCTACAAAGACATAATCCCCGAAAATAAGAACTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATa                                                                                                                                                                                                                <  1:47447/150‑1 (MQ=255)                                                                                                                                  ggtggtGTAGTGAGAATGAATACCGTAGGCTACAAAGACATAATCCCCGAAAATAAGAACTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCaaaaa                                                                                                                                   >  1:873119/1‑227 (MQ=255)                                                                                                                                      gtgtAGTGAGAATGAATACCGTAGGCTACAAAGACATAATCCCCGAAAATAAGAACTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCAAAAATCCTTTTCAGAATTTAGGCAAAAGCTa                                                                                                        >  1:584269/1‑250 (MQ=255)                                                                                                                                         tAGTGAGAATGAATCCCGTAGGCTACAAAGACATAATCCCCGAAAATAAGAACGGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCAAAAATCCTTTTCAGAATTTAGGCAAAAGCTACAac                                                                                                    <  1:141850/251‑1 (MQ=255)                                                                                                                                         tAGTGAGAATGAATACCGTAGGCTACAAAGACATAATCCCCGAAAATAAGAACTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCAAAAATCCTTTTCAGAATTTAGGCAAAAGCTACAac                                                                                                    <  1:755958/251‑1 (MQ=255)                                                                                                                                         tAGTGAGAATGAATACCGTAGGCTACAAAGACATAATCCCCGAAAATAAGAACTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCAAAAATCCTTTTCAGAATTTAGGCAAAAGCTACAac                                                                                                    <  1:658669/251‑1 (MQ=255)                                                                                                                                           gTGAGAATGAATACCGTAGGCTACAAAGACATAATCCCCGAAAATAAGAACTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCAAAAATCCTTTTCAGAATTTAGGCAAAAGCTACAacta                                                                                                  >  1:570042/1‑251 (MQ=255)                                                                                                                                           gTGAGAATGAATACCGTAGGCTACAAAGACATAATCCCCGAAAATAAGAACTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCAAAAATCCTTTTCAGAATTTAGGCAAAAGCTACAacta                                                                                                  >  1:472970/1‑251 (MQ=255)                                                                                                                                              agaATGAATACCGTAGGCTACAAGGACATAATCCCCGAAAATAAGAACTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCAAAAATCCTTTTCAGAATTTAGGCAAAAGCTACAactact                                                                                                <  1:653981/250‑1 (MQ=255)                                                                                                                                               gaatgaatACCGTAGGCTACAAAGACATAATCCCCGAAAATAAGAACTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCAAAAATCCTTTTCAGAATTTAGGCAAAAGCTACAACTActcg                                                                                              >  1:425475/1‑251 (MQ=255)                                                                                                                                                aatgaatACCGTAGGCTACAAAGACATAATCCCCGAAAATAAGAACTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCAAAAATCCTTTTCAGAATTTAGGCAAAAGCTACAACTActcg                                                                                              >  1:76316/1‑250 (MQ=255)                                                                                                                                                   gaatACCGTAGGCTACAAAGACATAATCCCCGAAAATAAGAACTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATAc                                                                                                                                              >  1:621749/1‑199 (MQ=255)                                                                                                                                                   gaatACCGTAGGCTACAAAGACATAATCCCCGAAAATAAGAACTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCAAAAATCCTTTTCAGAATTTAGGCAAAAGCTACAACTActcgctcg                                                                                          >  1:63761/1‑251 (MQ=255)                                                                                                                                                     atACCGTAGGCTACAAAGACATAATCCCCGAAAATAAGAACTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCAAAAATCCTTTTCAGAATTTAGGCAAAAGCTACAactac                                                                                                 <  1:540866/242‑1 (MQ=255)                                                                                                                                                             ggCTACAAAGACATAATCCCCGAAAATAAGAACTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCAAAAATCCTTTTCAGAATTTAGGCAAAAGCTACAACTACTCGCTCGCATTTTCTaa                                                                                >  1:677263/1‑251 (MQ=255)                                                                                                                                                               cTACAAAGACATAATCCCCGAAAATAAGAACTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCAAAAATCCTTTTCAGAATTTAGGCAAAAGCTACAac                                                                                                    <  1:575219/229‑1 (MQ=255)                                                                                                                                                                       aCATAATCCCCGAAAATAAGAACTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCAAAAATCCTTTTCAGAATTTAGGCAAAAGCTACAACTACTCGCTCGCATTTTCTAAAAAGTGGGaa                                                                      >  1:640276/1‑251 (MQ=255)                                                                                                                                                                                   aaaaTAAGAACTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCAAAAATCCTTTTCAGAATTTAGGCAAAAGCTACAACTACTCGCTCGCATTTTCTAAAAAGTGGGAAAACGCCGACAt                                                           <  1:194600/250‑1 (MQ=255)                                                                                                                                                                                     aaaaaGAGCTGGGGACTTCGCTCGCGGTGCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCAAAAATCCTTTTCAGAATTTAGGCAAAAGCTACAac                                                                                                    <  1:638492/204‑1 (MQ=255)                                                                                                                                                                                     aaTAAGAACTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCAAAAATCCTTTTCAGAATTTAGGCAAAAGCTACAACTACTCGCTCGCATTTTCTAAAAAGTGGGAAAACGCCGAc                                                             <  1:685088/246‑1 (MQ=255)                                                                                                                                                                                     aaTAAGAACTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCAAAAATCCTTTTCAGAATTTAGGCAAAAGCTACAACTACTCGCTCGCATTTTCTAAAAAGTGGGAAAACGCCGACAt                                                           <  1:413240/248‑1 (MQ=255)                                                                                                                                                                                       tAAGAACTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCAAAAATCCTTTTCAGAATTTAGGCAAAAGCTACAACTACTCGCTCGCATTTTCTAAAAAGTGGGAAAACGCCGACATTgtgt                                                      <  1:114356/251‑1 (MQ=255)                                                                                                                                                                                          gAACTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAAc                                                                                                                                                             >  1:682511/1‑145 (MQ=255)                                                                                                                                                                                           aaCTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCAAAAATCCTTTTCAGAATTTAGGCAAAAGCTACAACTACTCGCTCGCATTTTCTAAAAAGTGGGAAAACGCCGACATTGTGTTgg                                                   >  1:278763/1‑250 (MQ=255)                                                                                                                                                                                            aCTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCAAAAATCCTTTTCAGAATTTAGGCAAAAGCTACAACTACTCGCTCGCATTTTCTAAAAAGTGGGAAAACGCCGACATTGTGTTGGcc                                                 <  1:689922/251‑1 (MQ=255)                                                                                                                                                                                            aCTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCAAAAATCCTTTTCAGAATTTAGGCAAAAGCTACAACTACTCGCTCGCATTTTCTAAAAAGTGGGAAAACGCCGACATTGTGTTGGcc                                                 <  1:369490/251‑1 (MQ=255)                                                                                                                                                                                            aCTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCAAAAATCCTTTTCAGAATTTAGGCAAAAGCTACAACTACTCGCTCGCATTTTCTAAAAAGTGGGAAAACGCCGACATTGTGTTGGc                                                  >  1:109931/1‑250 (MQ=255)                                                                                                                                                                                                               gTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAAcgc                                                                                                                                                     <  1:895449/132‑1 (MQ=255)                                                                                                                                                                                                               gTTCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCAAAAATCCTTTTCAGAATTTAGGCAAAAGCTACAac                                                                                                    <  1:534265/181‑1 (MQ=255)                                                                                                                                                                                                                 tCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCAAAAATCCTTTTCAGAATTTAGGCAAAAGCTACAACTACTCGCTCGCATTTTCTAAAAAGTGGGAAAACGCCGACATTGTGTTGGCCTACGCCAAAAAGAAGCAGGGc                            <  1:439491/251‑1 (MQ=255)                                                                                                                                                                                                                 tCGGCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACAAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCAAAAATCCTTTTCAGAATTTAGGCAAAAGCTACAACTACTCGCTCGCATTTTCTAAAAAGTGGGAAAACGCCGACATTgtgt                                                      >  1:185326/1‑225 (MQ=255)                                                                                                                                                                                                                    gCACGATGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCAAAAATCCTTTTCAGAATTTAGGCAAAAGCTACAACTACTCGCTCGCATTTTCTAAAAAGTGGGAAAACGCCGACATTGTGTTGGCCTACGCCAAAAAGAAGCAGGGCatt                         >  1:637886/1‑249 (MQ=255)                                                                                                                                                                                                                         aTGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCAAAAATCCTTTTCAGAATTTAGGCAAAAGCTACAac                                                                                                    >  1:5995/1‑171 (MQ=255)                                                                                                                                                                                                                         aTGTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCAAAAATCCTTTTCAGAATTTAGGCAAAAGCTACAACTACTCGCTCGCATTTTCTAAAAAGTGGGAAAACGCCGACATTGTGTTGGCCTACGCCAAAAAGAAGc                                 <  1:203639/238‑1 (MQ=255)                                                                                                                                                                                                                           gTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCAAAAATCCTTTTCAGAATTTAGGCAAAAGCTACAACTACTCGCTCGCATTTTCTAAAAAGTGGGAAAACGCCGACATTGTGTTGGCCTACGCCAAAAAGAAGc                                 <  1:568683/236‑1 (MQ=255)                                                                                                                                                                                                                           gTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCAAAAATCCTTTTCAGAATTTAGGCAAAAGCTACAACTACTCGCTCGCATTTTCTAAAAAGTGGGAAAACGCCGACATTGTGTTGGCCTACGCCAAAAAGAAGCAGGGCAATTATTtcg                  >  1:726824/1‑251 (MQ=255)                                                                                                                                                                                                                           gTCTAATACTACAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCAAAAATCCTTTTCAGAATTTAGGCAAAAGCTACAACTACTCGCTCGCATTTTCTAAAAAGTGGGAAAACGCCGACATTGTGTTGGCCTACGCCAAAAAGAAGCAGGGCAATTATTtcg                  >  1:25632/1‑251 (MQ=255)                                                                                                                                                                                                                                 tactacAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCAAAAATCCTTTTCAGAATTTAGGCAAAAGCTACAACTACTCGCTCGCATTTTCTAAAAAGTGGGAAAACGCCGACATTGTGTTGGCCTACGCCAAAAAGAAGCAGGGCAATTATTTCGTCGGAc            <  1:611467/251‑1 (MQ=255)                                                                                                                                                                                                                                  actacAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCAAAAATCCTTTTCAGAATTTAGGCAAAAGCTACAACTACTCGCTCGCATTTTCTAAAAAGTGGGAAGACGCCGACATTGTGTTGGCCTACGCCAAAAAGAAGCAGGGCAATTATTTCGTCGGACg           >  1:46890/1‑251 (MQ=255)                                                                                                                                                                                                                                  actacAGACAAACCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCAAAAATCCTTTTCAGAATTTAGGCAAAAGCTACAACTACTCGCTCGCATTTTCTAAAAAGTGGGAAAACGCCGACAt                                                           >  1:701347/1‑203 (MQ=255)                                                                                                                                                                                                                                           aaaCCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCAAAAATCCTTTTCAGAATTTAGGCAAAAGCTACAACTACTCGCTCGCATTTTCTAAAAAGTGGGAAAACGCCGACATTGTGTTGGCCTACGCCAAAAAGAAGCAGGGCAATTATTTCGTCGGACGCCATGgcgc  <  1:218802/251‑1 (MQ=255)                                                                                                                                                                                                                                           aaaCCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCAAAAATCCTTTTCAGAATTTAGGCAAAAGCTACAACTACTCGCTCGCATTTTCTAAAAAGTGGGAAAACGCCGACATTGTGTTGGCCTACGCCAAAAAGAAGCAGGGCAATTATTTCGTCGGACGCCATGgcgc  <  1:849158/251‑1 (MQ=255)                                                                                                                                                                                                                                           aaaCCGCCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCAAAAATCCTTTTCAGAATTTAGGCAAAAGCTACAACTACTCGCTCGCATTTTCTAAAAAGTGGGAAAACGCCGACATTGTGTTGGCCTACGCCAAAAAGAAGCAGGGCAATTATTTCGTCGGACGCCATGgcgc  <  1:59354/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                      CTTCAGTCTATCCCTTCGTGGCAAGGCTATGCAGGTTCAAGTACCCGAACATATCTGGATCCTGATCTTATCTCGCGCGTAGAAATAGAAAAAGGCCCGTCACTCAAAGCAGATGCTACCGGCGCCACAGGTGGTGTAGTGAGAATGAATACCGTAGGCTACAAAGACATAATCCCCGAAAATAAGAACTGGGGACTTCGCTTGCGGTTCGGCACGATGTCTAATACTACAGACAAACCACCTCTTTATACACGCGGCGGATACCGCACTAAATGGATAGACGAATGTCGTACCAACCGTTCGGGTAAATGTCCGAAGCAAACCTACAACCCCAACGCGAAATACGCATCCAAAAATCCTTTTCAGAATTTAGGCAAAAGCTACAACTACTCGCTCGCATTTTCTAAAAAGTGGGAAAACGCCGACATTGTGTTGGCCTACGCCAAAAAGAAGCAGGGCAATTATTTCGTCGGACGCCATGGCGC  >  1/1793599‑1794083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑