Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,752,738	G→A	intergenic (+110380/‑28987)	unknown → / → unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,752,738	0	G	A	100.0%	209.5 / NA	74	intergenic (+110380/‑28987)	unknown/unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/38);  total (36/38)

TTTTTATTGTGAAAGTGCTAAGATAAGAAAAATAATACGCAAAAAGGAGATTTTATGTCATCCCACATTTCAGACAAGATTCAAGATGCTTTAGGCAAGATAGAACATTCCGTCTTGGCACAAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTGCCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGCATCGGTGGCGATTTTTATTTCGGTGCCAATCAGGAATTTTGCCAAACGGACATTCAACAAACTATTCATGCGGAACAAAGTGCCATTAGCCACGCTTGGATGCGCGGTGAAAAACAACTGACCGATGTCACGGTAAACTACACTCCGTGCGGGCATTGTCGCCAGTTTATGAA  >  1/1752499‑1752977                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                tttttATTGTGAAAGTGCTAAGATAAGAAAAATAATACGCAAAAAGGAGATTTTATGTCATCCCACATTTCAGACAAGATTCAAGATGCTTTAGGCAAGATAGAACATTCCGTCTTGGCACAAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTg                                                                                                                                                                                                                                       >  1:211743/1‑250 (MQ=255)               gTGCTAAGATAAGAAAAATAATACGCAAAAAGGAGATTTTATGTCATCCCACATTTCAGACAAGATTCAAGATGCTTTAGGCAAGATAGAACATTCCGTCTTGGCACAAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGccacca                                                                                                                                                                                                                        >  1:829862/1‑251 (MQ=255)               gTGCTAAGATAAGAAAAATAATACGCAAAAAGGAGATTTTATGTCATCCCACATTTCAGACAAGATTCAAGATGCTTTAGGCAAGATAGAACATTCCGTCTTGGCACAAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGcc                                                                                                                                                                                                                            <  1:168005/247‑1 (MQ=255)                tGCTAAGATAAGAAAAATAATACGCAAAAAGGAGATTTTATGTCATCCCACATTTCAGACAAGATTCAAGATGCTTTAGGCAAGATAGAACATTCCGTCTTGGCACAAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGccacca                                                                                                                                                                                                                        >  1:727706/1‑250 (MQ=255)                      gataagaAAAATAATACGCAAAAAGGAGATTTTATGTCATCCCACATTTCAGACAAGATTCAAGATGCTTTAGGCAAGATAGAACATTCCGTCTTGGCACAAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGAt                                                                                                                                                                                                                 >  1:780534/1‑251 (MQ=255)                           gaAAAATAATACGCAAAAAGGAGATTTTATGTCATCCCACATTTCAGACAAGATTCAAGATGCTTTAGGCAAGATAGAACATTCCGTCTTGGCACAAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGc                                                                                                                                                                                                                                         <  1:688746/222‑1 (MQ=255)                                    taCGCAAAAAGGAGATTTTATGTCATCCCACATTTCAGACAAGATTCAAGATGCTTTAGGCAAGATAGAACATTCCGTCTTGGCACAAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAAtg                                                                                                                                                                                                   <  1:95591/251‑1 (MQ=255)                                    taCGCAAAAAGGAGATTTTATGTCATCCCACATTTCAGACAAGATTCAAGATGCTTTAGGCAAGATAGAACATTCCGTCTTGGCACAAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAAtg                                                                                                                                                                                                   <  1:884453/251‑1 (MQ=255)                                        cAAAAAGGAGATTTTATGTCATCCCACATTTCAGACAAGATTCAAGATGCTTTAGGCAAGATAGAACATTCCGTCTTGGCACAAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTggg                                                                                                                                                                                               >  1:113781/1‑251 (MQ=255)                                                 gaTTTTATGTCATCCCACATTTCAGACAAGATTCAAGATGCTTTAGGCAAGATAGAACATTCCGTCTTGGCACAAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGt                                                                                                                                                                                      >  1:717218/1‑251 (MQ=255)                                                  aTTTTATGTCATCCCACATTTCAGACAAGATTCAAGATGCTTTAGGCAAGATAGAACATTCCGTCTTGGCACAAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAAtg                                                                                                                                                                                                   <  1:419720/237‑1 (MQ=255)                                                  aTTTTATGTCATCCCACATTTCAGACAAGATTCAAGATGCTTTAGGCAAGATAGAACATTCCGTCTTGGCACAAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTa                                                                                                                                                                                     >  1:196156/1‑251 (MQ=255)                                                   ttttATGTCATCCCACATTTCAGACAAGATTCAAGATGCTTTAGGCAAGATAGAACATTCCGTCTTGGCACAAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTAc                                                                                                                                                                                    <  1:299394/251‑1 (MQ=255)                                                   ttttATGTCATCCCACATTTCAGACAAGATTCAAGATGCTTTAGGCAAGATAGAACATTCCGTCTTGGCACAAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTAc                                                                                                                                                                                    <  1:591134/251‑1 (MQ=255)                                                     ttATGTCATCCCACATTTCAGACAAGATTCAAGATGCTTTAGGCAAGATAGAACATTCCGTCTTGGCACAAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCGCATTTTAATGTGGGTGCGGTTGTACAt                                                                                                                                                                                  <  1:157502/251‑1 (MQ=255)                                                     ttATGTCATCCCACATTTCAGACAAGATTCAAGATGCTTTAGGCAAGATAGAACATTCCGTCTTGGCACAAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACAt                                                                                                                                                                                  <  1:479810/251‑1 (MQ=255)                                                     ttATGTCATCCCACATTTCAGACAAGATTCAAGATGCTTTAGGCAAGATAGAACATTCCGTCTTGGCACAAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACAt                                                                                                                                                                                  <  1:400898/251‑1 (MQ=255)                                                        tGTCATCCCACATTTCAGACAAGATTCAAGATGCTTTAGGCAAGATAGAACATTCCGTCTTGGCACAAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGc                                                                                                                                                                               <  1:627279/251‑1 (MQ=255)                                                          tCATCCCACATTTCAGACAAGATTCAAGATGCTTTAGGCAAGATAGAACATTCCGTCTTGGCACAAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACAt                                                                                                                                                                                  <  1:380603/246‑1 (MQ=255)                                                           cATCCCACATTTCAGACAAGATTCAAGATGCTTTAGGCAAGATAGAACATTCCGTCTTGGCACAAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGCAt                                                                                                                                                                             >  1:687341/1‑250 (MQ=255)                                                             tCCCACATTTCAGACAAGATTCAAGATGCTTTAGGCAAGATAGAACATTCCGTCTTGGCACAAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGCATCgg                                                                                                                                                                          <  1:274953/251‑1 (MQ=255)                                                             tCCCACATTTCAGACAAGATTCAAGATGCTTTAGGCAAGATAGAACATTCCGTCTTGGCACAAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGCATCgg                                                                                                                                                                          <  1:635771/251‑1 (MQ=255)                                                                  caTTTCAGACAAGATTCAAGATGCTTTAGGCAAGATAGAACATTCCGTCTTGGCACAAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGCTTTCTACCGCACTTTGCCGTGCAACACCTGGGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAAtg                                                                                                                                                                                                   >  1:550465/1‑221 (MQ=255)                                                                    tttCAGACAAGATTCAAGATGCTTTAGGCAAGATAGAACATTCCGTCTTGGCACAAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACAt                                                                                                                                                                                  <  1:223693/236‑1 (MQ=255)                                                                    tttCAGACAAGATTCAAGATGCTTTAGGCAAGATAGAACATTCCGTCTTGGCACAAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGCATCGGTGGCGAt                                                                                                                                                                   >  1:34168/1‑251 (MQ=255)                                                                             aGATTCAAGATGCTTTAGGCAAGATAGAACATTCCGTCTTGGCACAAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCCGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTcaca                                                                                                                                                                                                           <  1:91062/202‑1 (MQ=255)                                                                               aTTCAAGATGCTTTAGGCAAGATAGAACATTCCGTCTTGGCACAAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGCATCGGTGGCGATTTTTATTTCgg                                                                                                                                                        >  1:481585/1‑251 (MQ=255)                                                                                       tGCTTTAGGCAAGATAGAACATTCCGTCTTGGCACAAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGCATCGGTGGCGATTTTTATTTCGGTGCCAATc                                                                                                                                                <  1:455546/251‑1 (MQ=255)                                                                                         cTTTAGGCAAGATAGAACATTCCGTCTTGGCACAAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGCATCGGTGGCGATTTTTATTTCGGTGCCAATCAg                                                                                                                                              >  1:648327/1‑251 (MQ=255)                                                                                             aGGCAAGATAGAACATTCCGTCTTGGCACAAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGc                                                                                                                                                                               >  1:496824/1‑214 (MQ=255)                                                                                                  aGATAGAACATTCCGTCTTGGCACAAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATg                                                                                                                                                                                                                              >  1:539271/1‑162 (MQ=255)                                                                                                  aGATAGAACATTCCGTCTTGGCACAAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACAt                                                                                                                                                                                  <  1:893518/206‑1 (MQ=255)                                                                                                  aGATAGAACATTCCGTCTTGGCACAAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGCATCGGTGGCGATTTTTATTTCGGTGCCAATCAGGaa                                                                                                                                           >  1:301822/1‑245 (MQ=255)                                                                                                  aGATAGAACATTCCGTCTTGGCACAAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGCATCGGTGGCGATTTTTATTTCGGTGCCAATCAGGAATTTTg                                                                                                                                      >  1:681322/1‑250 (MQ=255)                                                                                                  aGATAGAACATTCCGTCTTGGCACAAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGCGCGGTTGTACATGGCATCGGTGGCGATTTTTATTTCGGTGCCAATCAGGAATTTTg                                                                                                                                      >  1:408991/1‑250 (MQ=255)                                                                                                          cATTCCGTCTTGGCACAAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTg                                                                                                                                                                                       >  1:641698/1‑193 (MQ=255)                                                                                                           aTTCCGTCTTGGCACAAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGCATCGGTGGTGATTTTTATTTCGGTGCCAATCAGGAATTTTGCCAAACGGa                                                                                                                             >  1:349112/1‑250 (MQ=255)                                                                                                              ccGTCTTGGCACAAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAGCAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGCATCGGTGGCGATTTTTATTTCGGTGCCAATCAGGAATTTTGCCAAACGGACAtt                                                                                                                         <  1:303497/251‑1 (MQ=255)                                                                                                                gTCTTGGCACAAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGCATCGGTGGCGATTTTTATTTCGGTGCCAATCAGGAATTTTGCCAAACGGACATTca                                                                                                                       >  1:293747/1‑251 (MQ=255)                                                                                                                 tCTTGGCACAAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGCATCGGTGGCGATTTTTATTTCGGTGCCAATCAGGAATTTTGCCAAACGGACATTcaa                                                                                                                      >  1:296391/1‑251 (MQ=255)                                                                                                                         caAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGCATCGGTGGCGATTTTTATTTCGGTGCCAATCAGGAATTTTGCCAAACGGACATTCAACAAACTAt                                                                                                              <  1:587026/251‑1 (MQ=255)                                                                                                                           aGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTggg                                                                                                                                                                                               >  1:881345/1‑168 (MQ=255)                                                                                                                           aGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACAt                                                                                                                                                                                  <  1:26741/181‑1 (MQ=255)                                                                                                                                        atTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGCATCGGTGGCGATTTTTATTTCGGTGCCAATCAGGAATTTTGCCAAACGGACATTCAACAAACTATTCATGCGGAACAAAg                                                                                               >  1:843403/1‑251 (MQ=255)                                                                                                                                                 gAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGCATCGGTGGCGATTTTTATTTCGGTGCCAATCAGGAATTTTGCCAAACGGACATTCAACAAACTATTCATGCGGAACAAAGTGCCATTAg                                                                                      >  1:873222/1‑251 (MQ=255)                                                                                                                                                  aaCAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAAtgt                                                                                                                                                                                                  <  1:199368/142‑1 (MQ=255)                                                                                                                                                    cAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACAt                                                                                                                                                                                  <  1:51844/156‑1 (MQ=255)                                                                                                                                                    cAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGCATCGGTGGCGATTTTTATTTCGGTGCCAATCAGGAATTTTGCCAAACGGACATTCAACAAACTATTCATGCGGAACAAAGTGCCATTAGCCa                                                                                   >  1:846175/1‑251 (MQ=255)                                                                                                                                                               cGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGCATCGGTGGCGATTTTTATTTCGGTGCCAATCAGGAATTTTGCCAAACGGACATTCAACAAACTATTCATGCGGAACAAAGTGCCATTAGCCACGCTTGGATgc                                                                        <  1:520340/251‑1 (MQ=255)                                                                                                                                                                 gATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGTTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGCATCGGTGGCGATTTTTATTTCGGTGCCAATCAGGAATTTTGCCAAACGGACATTCAACAAACTATTCATGCGGAACAAAGTGCCAt                                                                                         <  1:427793/232‑1 (MQ=255)                                                                                                                                                                 gATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAAtg                                                                                                                                                                                                   >  1:756387/1‑126 (MQ=255)                                                                                                                                                                      cTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGCATCGGTGGCGATTTTTATTTCGGTGCCAATCAGGAATTTTGCCAAACGGACATTCAACAAACTATTCATGCGGAACAAAGTGCCATTAGCCACGCTTGGATGCGCGGTGa                                                                 >  1:656641/1‑251 (MQ=255)                                                                                                                                                                                     gTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGCATCGGTGGCGATTTTTATTTc                                                                                                                                                          <  1:803448/147‑1 (MQ=255)                                                                                                                                                                                      tGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGCATCGGTGGCGATTTTTATTTCGGTGCCAATCAGGAATTTTGCCAAACGGACATTCAACAAACTATTCATGCGGAACAAAGTGCCATTAGc                                                                                     >  1:884956/1‑215 (MQ=255)                                                                                                                                                                                        cAACACCTGTGCAACAAACATCAACT‑‑CGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGCATCGGTGGCGATTTTTATTTCGGTGCCAATCAGGAATTTTGCCAAACGGACATTCAACAAACTATTCATGCGGAACAAAGTGCCATTAGCCACGCTTGGATGCGCGGTGAAAAACAACTGACCGATGTc                                              <  1:280214/250‑1 (MQ=255)                                                                                                                                                                                                   caacaaACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGCATCGGTGGCGATTTTTATTTCGGTGCCAATCAGGAATTTTGCCAAACGGACATTCAACAAACTATTCATGCGGAACAAAGTGCCATTAGCCACGCTTGGATGCGCGGTGAAAAACAACTGACCGATGTCACGGTAAACt                                    <  1:687686/251‑1 (MQ=255)                                                                                                                                                                                                     acaaacaTCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGCATCGGTGGCGATTTTTATTTCGGTGCCAATCAGGAATTTTGCCAAACGGACATTCAACAAACTATTCATGCGGAACAAAGTGCCATTAGCCACGCTTGGATGCGCGGTGAAAAACAACTGACCGATGTCACGGTAAACTac                                  <  1:697027/251‑1 (MQ=255)                                                                                                                                                                                                     acaaacaTCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGCATCGGTGGCGATTTTTATTTCGGTGCCAATCAGGAATTTTGCCAAACGGACATTCAACAAACTATTCATGCGGAACAAAGTGCCATTAGCCACGCTTGGATGCGCGGTGAAAAACAACTGACCGATGTCACGGTAAACTac                                  <  1:620029/251‑1 (MQ=255)                                                                                                                                                                                                         acaTCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGCATCGGTGGCGATTTTTATTTCGGTGCCAATCAGGAATTTTGCCAAACGGACATTCAACAAACTATTCATGCGGAACAAAGTGCCATTAGCCACGCTTGGATGCGCGGTGAAAAACAACTGACCGATGTCACGGTAAACTACACTc                              <  1:628547/251‑1 (MQ=255)                                                                                                                                                                                                         acaTCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGCATCGGTGGCGATTTTTATTTCGGTGCCAATCAGGAATTTTGCCAAACGGACATTCAACAAACTATTCATGCGGAACAAAGTGCCATTAGCCACGCTTGGATGCGCGGTGAAAAACAACTGACCGATGTCACGGTAAACTACACTc                              <  1:790704/251‑1 (MQ=255)                                                                                                                                                                                                         acaTCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGCATCGGTGGCGATTTTTATTTCGGTGCCAATCAGGAATTTTGCCAAACGGACATTCAACAAACTATTCATGCGGAACAAAGTGCCATTAGCCACGCTTGGATGCGCGGTGAAAAACAACTGACCGATGTCACGGTAAACTACACTc                              <  1:95204/251‑1 (MQ=255)                                                                                                                                                                                                                    cGCCTAAACAGCTCGCGCTGAAACTCCTACTTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGCATCGGTGGCGATTTTTATTTCGGTGCCAATCAGGAATTTTGCCAAACGGACATTCAACAAACTATTc                                                                                                            >  1:4639/1‑162 (MQ=255)                                                                                                                                                                                                                    cGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGc                                                                                                                                                                                                                                         >  1:191548/1‑37 (MQ=255)                                                                                                                                                                                                                    cGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGCATCGGTGGCGATTTTTATTTCGGTGCCAATCAGGAATTTTGCCAAACGGACATTCAACAAACTATTCATGCGGAAc                                                                                                   <  1:76317/171‑1 (MQ=255)                                                                                                                                                                                                                    cGCCTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGCATCGGTGGCGATTTTTATTTCGGTGCCAATCAGGAATTTTGCCAAACGGACATTCAACAAACTATTCATGCGGAACAAAGTGCCATTAGCCACGCTTGGATGCGCGGTGAAAAACAACTGACCGATGTCACGGTAAACTACACTCCGTGCGGGCAt                   >  1:737404/1‑251 (MQ=255)                                                                                                                                                                                                                      ccTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCTTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGCATCGGTGGCGATTTTTATTTCGGTGCCAATCAGGAATTTTGCCAAACGGACATTCAACAAACTATTCATGCGGAACAAAGTGCCATTAGCCACGCTTGGATGCGCGGTGAAAAACAACTGACCGATGTCACGGTAAACTacac                                <  1:536255/236‑1 (MQ=255)                                                                                                                                                                                                                      ccTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGCATCGGTGGCGATTTTTATTTCGGTGCCAATCAGGAATTTTGCCAAACGGACATTCAACAAACTATTCATGCGGAACGAAGTGCCATTAGCCACGCTTGGATGCGCGGTGAAAAACAACTGACCGATGTCACGGTAAACTACACTCCGTGCGGGCATTg                 >  1:451634/1‑251 (MQ=255)                                                                                                                                                                                                                      ccTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGCATCGGTGGCGATTTTTATTTCGGTGCCAATCAGGAATTTTGCCAAACGGACATTCAACAAACTATTCATGCGGAACAAAGTGCCATTAGCCACGCTTGGATGCGCGGTGAAAAACAACTGACCGATGTCACGGTAAACTACACTCCGTGCGGGCATTg                 >  1:105757/1‑251 (MQ=255)                                                                                                                                                                                                                      ccTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGCATCGGTGGCGATTTTTATTTCGGTGCCAATCAGGAATTTTGCCAAACGGACATTCAACAAACTATTCATGCGGAACAAAGTGCCATTAGCCACGCTTGGATGCGCGGTGAAAAACAACTGACCGATGTCACGGTAAACTACACTCCGTGCGGGCATTg                 >  1:690617/1‑251 (MQ=255)                                                                                                                                                                                                                      ccTAAACAGCTCGCGCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGCATCGGTGGCGATTTTTATTTCGGTGCCAATCAGGAATTTTGCCAAACGGACATTCAACAAACTATTCATGCGGAACAAAGTGCCATTAGCCACGCTTGGATGCGCGGTGAAAAACAACTGACCGATGTCACGGTAAACTACACTCCGTGCGGGCATTg                 >  1:649137/1‑251 (MQ=255)                                                                                                                                                                                                                                 cgcgCTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAAtg                                                                                                                                                                                                   >  1:114145/1‑62 (MQ=255)                                                                                                                                                                                                                                   cgcTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGCATCGGTGGCGATTTTTATTTCGGTGCCAATCAGGAATTTTGCCAAACGGACATTCAACAAACTATTCATGCGGAACAAAGTGCCATTAGCCACGCTTGGATGCGCGGTGAAAAACAACTGACCGATGTCACGGTAAACTACACTCCGTGCGGGCATTGTCGCCAGTTTatg    <  1:30053/251‑1 (MQ=255)                                                                                                                                                                                                                                     cTGAAACTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGCATCGGTGGCGATTTTTATTTCGGTGCCAATCAGGAATTTTGCCAAACGGACATTCAACAAACTATTCATGCGGAACAAAGTGCCATTAGCCACGCTTGGATGCGCGGTGAAAAACAACTGACCGATGTCACGGTAAACTACACTCCGTGCGGGCATTGTCGCCAGTTTatgaa  <  1:412046/251‑1 (MQ=255)                                                                                                                                                                                                                                         aaCTCCTACCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGCATCGGTGGCGATTTTTATTTCGGTGCCAATCAGGAATTTTGCCAAACGGACATTCAACAAACTATTCATGCGGAACAAAGTGCCATTAGCCACGCTTGGATGCGCGGTGAAAaacaac                                                         <  1:235855/192‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                TTTTTATTGTGAAAGTGCTAAGATAAGAAAAATAATACGCAAAAAGGAGATTTTATGTCATCCCACATTTCAGACAAGATTCAAGATGCTTTAGGCAAGATAGAACATTCCGTCTTGGCACAAGATATTTGGCATATTTTGCAAGAACAAAAATACCTCGGATTTCTACCGCACTTTGCCGTGCAACACCTGTGCAACAAACATCAACTCACGCCTAAACAGCTCGCGCTGAAACTCCTGCCTATCGCTGCCTGTTATGCCACCACGCCGATTTCACATTTTAATGTGGGTGCGGTTGTACATGGCATCGGTGGCGATTTTTATTTCGGTGCCAATCAGGAATTTTGCCAAACGGACATTCAACAAACTATTCATGCGGAACAAAGTGCCATTAGCCACGCTTGGATGCGCGGTGAAAAACAACTGACCGATGTCACGGTAAACTACACTCCGTGCGGGCATTGTCGCCAGTTTATGAA  >  1/1752499‑1752977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑