Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	220,487	A→G	intergenic (‑6603/+59196)	unknown ← / ← unknown	tRNA‑Ser‑GCT/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	220,487	0	A	G	100.0%	110.7 / NA	44	intergenic (‑6603/+59196)	unknown/unknown	tRNA‑Ser‑GCT/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/33);  total (11/33)

TAAGATTCCTCATTCTCAAGTCATTTATTGGTTAAAAGCCTTTCGTGAAAGAGGGCTTGATGGCATAAAATCGCCTTATACTAAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAATTGGGGCGATCAAAAACAAATTAAACTCGATTTAAAAGTATTGAGCCTGGTACAATACCGAGCTCAATACTTC  >  1/220423‑220733                                                                 |                                                                                                                                                                                                                                                       ctaaatttctcgttCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTGATGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAATTGGGGCGATCaaaaa                                                           >  1:660182/13‑251 (MQ=11)  taaatttctcgttCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTGATGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAATTGGGGCGATCAAAAAc                                                          <  1:10064/240‑1 (MQ=11)  taaatttctcgttCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTGATGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAATTGGGGCGATCAAAAAc                                                          <  1:500863/240‑1 (MQ=11)  taaatttctcgttCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTGATGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAATTGGGGCGATCAAAAAc                                                          <  1:749024/240‑1 (MQ=11)  taaatttctcgttCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTGATGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAATTGGGGCGATCAAAAAc                                                          <  1:777479/240‑1 (MQ=11)   aaatttctcgttCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTGATGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAATTgggg                                                                     >  1:270846/11‑239 (MQ=11)           cgttCTCAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTGATGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAATTGGGGCGATCAAAAACAAATTAAAc                                                 <  1:349475/249‑1 (MQ=14)              tctcAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTGATGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAATTGGGGCGATCAAAAACAAATTAAACTCg                                              >  1:687968/1‑251 (MQ=255)               ctcAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTGATGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAATTGGGGCGATCAAAAAc                                                          >  1:736955/1‑238 (MQ=14)                 cAAGTCATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTGATGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAATTgggg                                                                     <  1:647501/225‑1 (MQ=11)                      cATTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTGATGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAAt                                                                          <  1:621030/215‑1 (MQ=11)                       aTTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTGATGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAATTGGGGCGATCAAAAACAAATTAAACTCGATTTAAAAg                                     <  1:240872/251‑1 (MQ=255)                       aTTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTGATGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAATTGGGGCGATCAAAAACAAATTAAACTCGATTTAAAAg                                     <  1:395212/251‑1 (MQ=255)                       aTTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTGATGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAATTGGGGCGATCAAAAACAAATTAAACTCGATTTAAAAg                                     <  1:472045/251‑1 (MQ=255)                       aTTTATTGG‑‑AAAAGCCTTTCGTGAAAGAGGGCTTGATGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAAAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAATTGGGGCGATCAAAAACAAATTAAACTCGATTTAAAAg                                     <  1:155611/251‑1 (MQ=17)                            tattggaaaaGCCTTTCGTGAAAGAGGGCTTGATGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAATTGGGGCGATCAAAAACAAATTAAACTCGATTTAAAAGTAt                                  <  1:329574/245‑1 (MQ=255)                                ggaaaaGCCTTTCGTGAAAGAGGGCTTGATGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAATTGGGGCGATCAAAAACAAATTAAACTCGATTTAAAAGTATTGAg                              >  1:472157/3‑251 (MQ=255)                                  aaaaGCCTTTCGTGAAAGAGGGCTTGATGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAATTGGGGCGATCAAAAACAAATTAAACTCGATTTAAAAGTATTGAGc                             <  1:730491/250‑1 (MQ=255)                                     aGCCTTTCGTGAAAGAGGGCTTGATGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAATTgggg                                                                     <  1:803867/207‑1 (MQ=255)                                      gCCTTTCGTGAAAGAGGGCTTGATGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAAccc                                                                                                                        <  1:110507/155‑1 (MQ=11)                                      gCCTTTCGTGAAAGAGGGCTTGATGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAATTGGGGCGATCAAAAACAAATTAAACTCGATTTAAAAGTATTGAGCCTGGt                        >  1:112192/1‑251 (MQ=255)                                        cTTTCGTGAAAGAGGGCTTGATGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAATTGGGGCGATCAAAAACAAATTAAACTCGATTTAAAAGTATTGAGCCTGGTAc                      <  1:44344/251‑1 (MQ=255)                                        cTTTCGTGAAAGAGGGCTTGATGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAATTGGGGCGATCAAAAACAAATTAAACTCGATTTAAAAGTATTGAGCCTGGTAc                      <  1:315628/251‑1 (MQ=255)                                        cTTTCGTGAAAGAGGGCTTGATGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAATTGGGGCGATCAAAAACAAATTAAACTCGATTTAAAAGTATTGAGCCTGGTAc                      <  1:513488/251‑1 (MQ=255)                                        cTTTCGTGAAAGAGGGCTTGATGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAATTGGGGCGATCAAAAACAAATTAAACTCGATTTAAAAGTATTGAGCCTGGTAc                      <  1:210409/251‑1 (MQ=255)                                        cTTTCGTGAAAGAGGGCTTGATGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAATTGGGGCGATCAAAAACAAATTAAACTCGATTTAAAAGTATTGAGCCTGGTAc                      <  1:727300/251‑1 (MQ=255)                                        cTTTCGTGAAAGAGGGCTTGATGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAATTGGGGCGATCAAAAACAAATTAAACTCGATTTAAAAGTATTGAGCCTGGTAc                      <  1:145750/251‑1 (MQ=255)                                          ttCGTGAAAGAGGGCTTGATGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAATTGGGGCGATCAAAAACAAATTAAACTCGATTTAAAAGTATTGAGcc                            >  1:717998/1‑243 (MQ=255)                                           tCGTGAAAGAGGGCTTGATGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACACAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAATTGGGGCGATCAAAAACAAATTAAACTCGATTTAAAAGTATTGAGCCTGGTACAAt                   >  1:271754/1‑251 (MQ=255)                                             gTGAAAGAGGGCTTGATGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTgg                                                                             >  1:892074/1‑191 (MQ=255)                                              tGAAAGAGGGCTTGATGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAGCCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAATTGGGGCGATCAAAAACAAATTAAACTCGGTTTAAAAGTATTGAGCCTGGTACAATAc                 <  1:578528/250‑1 (MQ=18)                                              tGAAAGAGGGCTTGATGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAATTGGGGCGATCAAAAACAAATTAAACTCGATTTAAAAGTATTGAGCCTGGTACAATAc                 <  1:893507/250‑1 (MQ=255)                                              tGAAAGAGGGCTTGATGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAATTGGGGCGATCAAAAACAAATTAAACTCGATTTAAAAGTATTGAGCCTGGTACAATAc                 <  1:80749/250‑1 (MQ=255)                                              tGAAAGAGGGCTTGATGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAATTGGGGCGATCAAAAACAAATTAAACTCGATTTAAAAGTATTGAGCCTGGTACAATAc                 <  1:139381/250‑1 (MQ=255)                                              tGAAAGAGGGCTTGATGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAATTGGGGCGATCAAAAACAAATTAAACTCGATTTAAAAGTATTGAGCCTGGTACAATAc                 <  1:140127/250‑1 (MQ=255)                                              tGAAAGAGGGCTTGATGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAATTGGGGCGATCAAAAACAAATTAAACTCGATTTAAAAGTATTGAGCCTGGTACAATAc                 <  1:575498/250‑1 (MQ=255)                                              tGAAAGAGGGCTTGATGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAATTGGGGCGATCAAAAACAAATTAAACTCGATTTAAAAGTATTGAGCCTGGTACAATAc                 <  1:545060/250‑1 (MQ=255)                                              tGAAAGAGGGCTTGATGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAATTGGGGCGATCAAAAACAAATTAAACTCGATTTAAAAGTATTGAGCCTGGTACAATAc                 <  1:380187/250‑1 (MQ=255)                                                    agGGCTTGATGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAATTGGGGCGATCAAAAACAAATTAAACTCGATTTAAAAGTATTGAGCCTGGTACAATACCGAGCt           >  1:186805/1‑250 (MQ=255)                                                    agGGCTTGATGGCGTAAAATCACCTTACAC‑AAACATAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAATTGGGGCGATCAAAAACAAATTAAACTCGATTTAAAAGTATTGAGCCTGGCACAATACCGAGc            <  1:295728/249‑1 (MQ=18)                                                     gggCTTGATGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAGAAAGTTAGTGGAATTGGGGCGATCAAAAACAAATTAAACTCGATTTAAAAGTATTGAGCCTGGTACAATACCGAGCTCa         >  1:748636/1‑251 (MQ=255)                                                      ggCTTGATGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAATTGGGGCGATCAAAAACAAATTAAACTCGATTTAAAAGTATTGAGCCTGGTACAATACCGAGCTc          <  1:800173/249‑1 (MQ=255)                                                       gCTTGATGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAATTgggg                                                                     <  1:267605/189‑1 (MQ=255)                                                            aTGGCGTAAAATCACCTTACAC‑AAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAATTGGGGCGATCAAAAACAAATTAAACTCGATTTAAAAGTATTGAGCCTGGTACAATACCGAGCTCAATACTTc  <  1:418947/251‑1 (MQ=255)                                                                 |                                                                                                                                                                                                                                                       TAAGATTCCTCATTCTCAAGTCATTTATTGGTTAAAAGCCTTTCGTGAAAGAGGGCTTGATGGCATAAAATCGCCTTATACTAAACCTAAAACCTCCAAAATTCTGAAACCCAAAATGAAAAAAACGCAGATTGAAGTGGCTGAAACAACCGACTTTTCACCACAAGCCTTTAAAAAACTGAAACGAGAACCCGCCCTTGAAAAAGCGGAGATTGATTATCTAAAAAAGTTAGTGGAATTGGGGCGATCAAAAACAAATTAAACTCGATTTAAAAGTATTGAGCCTGGTACAATACCGAGCTCAATACTTC  >  1/220423‑220733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑