Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,742,888 G→A intergenic (+100530/‑38837) unknown → / → unknown tRNA‑Leu‑CAA/tRNA‑Asp‑GTC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,742,8880GA100.0% 82.8 / NA 30intergenic (+100530/‑38837)unknown/unknowntRNA‑Leu‑CAA/tRNA‑Asp‑GTC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/14);  total (16/14)

AAACAAACCGATTTTTAACGGTTTGGCTTCCCCCTCAATAGAAAAACATAGCGGAAATTTTTCCGCTAAATAAGCAATAATCTCTTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCGCACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAATTTGGCCGACGCTACTTTTCATCGCCTCGTTCGTTGCGGTGAATGCAATGCCGTGGTTAAAATTTCTGCATTACAACGAGGGCAACGTGCCCTTTGTCCGCGCTGCCAGAATGTGTTATAT  >  1/1742647‑1743124
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                            
aaacaaacCGATTTTTAACGGTTTGGCTTCCCCCTCAATAGAAAAACATAGCGGAAATTTTTCCGCTAAATAAGCAATAATCTCTTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTc                                                                                                                                                                                                                                     <  1:35909/251‑1 (MQ=255)
                             cccccTCAATAGAAAAACATAGCGGAAATTTTTCCGCTAAATAAGCAATAATCTCTTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTaaaa                                                                                                                                                                                                        >  1:881364/1‑251 (MQ=255)
                                ccTCAATAGAAAAACATAGCGGAAATTTTTCCGCTAAATAAGCAATAATCTCTTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACg                                                                                                                                                                                                     >  1:157293/1‑251 (MQ=255)
                                        gAAAAACATAGCGGAAATTTTTCCGCTAAATAAGCAATAATCTCTTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAATAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTc                                                                                                                                                                                                                                     <  1:598252/211‑1 (MQ=255)
                                          aaaaCATAGCGGAAATTTTTCCGCTAAATAAGCAATAATCTCTTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAg                                                                                                                                                                                           >  1:809775/1‑251 (MQ=255)
                                                   cGGAAATTTTTCCGCTAAATAAGCAATAATCTCTTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAAt                                                                                                                                                                                   <  1:293081/250‑1 (MQ=255)
                                                   cGGAAATTTTTCCGCTAAATAAGCAATAATCTCTTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAAt                                                                                                                                                                                   <  1:604096/250‑1 (MQ=255)
                                                                                   ctTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGAtt                                                                                                                                                   >  1:315192/1‑250 (MQ=255)
                                                                                                            ttCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAAtt                                                                                                                         <  1:312416/251‑1 (MQ=255)
                                                                                                                      gCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATAc                                                                                                                                                                                             >  1:374209/1‑173 (MQ=255)
                                                                                                                                     gATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAata                                                                                                                                                                   >  1:313256/1‑184 (MQ=255)
                                                                                                                                          taataaGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAATTTGGCCGACGCTACTTTTCATCGCCtcgttc                                                                                           <  1:67662/251‑1 (MQ=255)
                                                                                                                                                gTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAATTTGGCCGACGCTACTTTTCATCGCCTCGTTCGTTGCg                                                                                     >  1:317869/1‑251 (MQ=255)
                                                                                                                                                           tACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGAc                                                                                                                                             >  1:515963/1‑184 (MQ=255)
                                                                                                                                                                tAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGt                                                                                                                                                            <  1:823970/164‑1 (MQ=255)
                                                                                                                                                                  gTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAATTTGGCCGACGCTACTTTTCATCGCCTCGTTCGTTGCGGTGAATGCAATGCCGTgg                                                                   >  1:123839/1‑251 (MQ=255)
                                                                                                                                                                   tttAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAATTTGGCCGACGCTACTTTTCATCGCCTCGTTCGTTGCGGTGaatgcaatgc                                                                        <  1:877365/245‑1 (MQ=255)
                                                                                                                                                                       gCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAATTTGGCCGACGCTACTTTTCAt                                                                                                     >  1:223171/1‑212 (MQ=255)
                                                                                                                                                                                           aCAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAATTTGGCCGACGCTACTTTTCATCGCCTCGTTCGTTGCGGTGAATGCAATGCCGTGGTTAAAATTTCTGCATTACAACGAgg                                          <  1:655056/251‑1 (MQ=255)
                                                                                                                                                                                              aTTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGta                                                                                                                                                                                         >  1:477018/1‑105 (MQ=255)
                                                                                                                                                                                               ttAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTc                                                                                                                                                                                                                 >  1:351350/1‑80 (MQ=255)
                                                                                                                                                                                                      tctatctaCTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAATTTGGCCGACGCTACTTTTCATCGCCTCGTTCGTTGCGGTGAATGCAATGCCGTGGTTAAAATTTCTGCATTACAACGAGGGCAACGTGccc                               <  1:55563/251‑1 (MQ=255)
                                                                                                                                                                                                           ctaCTGAAAAGAAATAATAAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAATTTGGCCGACGCTACTTTTCATCGCCTCGTTCGTTGCGGTGAATGCAATGCCGTGGTTAAAATTTCTGCATTACAACGAGGGCAACGTg                                  >  1:419572/1‑243 (MQ=255)
                                                                                                                                                                                                                        ataataAATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAATTTGGCCGACGCTACTTTTCATCGCCTCGTTCGTTGCGGTGAATGCAATGCCGTGGTTAAAATTTCTGCATTACAACGAGGGCAACGTGCCCTTTGTCCGCGCTGCCAGa             >  1:493482/1‑251 (MQ=255)
                                                                                                                                                                                                                          aataaATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAATTTGGCCGACGCTACTTTTCAt                                                                                                     >  1:64714/1‑161 (MQ=255)
                                                                                                                                                                                                                          aataaATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAATTTGGCCGACGCTACTTTTCATCGCCTCGTTCGTTGCGGTGAATGCAATGCCGTGGTTAAAAtt                                                           <  1:408338/203‑1 (MQ=255)
                                                                                                                                                                                                                          aataaATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAATTTGGCCGACGCTACTTTTCATCGCCTCGTTCGTTGCGGTGAATGCAATGCCGTGGTTAAAATTTCTGCATTACAACGAGGGCAACGTGCCCTTTGTCCGCGCTGCCAGAAt           >  1:621714/1‑251 (MQ=255)
                                                                                                                                                                                                                           ataaATTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAATTTGGCCGACGCTACTTTTCATCGCCTCGTTCGTTGCGGTGAATGCAATGCCGTGGTTAAAATTTCTGCATTACAACGAGGGCAACGTGCCCTTTGTCCGCGCTGCCAGAAtg          <  1:650315/251‑1 (MQ=255)
                                                                                                                                                                                                                              aaTTCCGCGAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAATTTGGCCGACGCTACTTTTCATCGCCTCGTTCGTTGCGGTGAATGCAATGCCGTGGTTAAAATTTCTGCATTACAACGAGGGCAACGTGCCCTTTGTCCGCGCTGCCAGAATgtgt       <  1:282218/251‑1 (MQ=255)
                                                                                                                                                                                                                                   cgcgAAAAATGACCACACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTGCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAATTTGGCCGACGCTACTTTTCATCGCCTCGTTCGTTGCGGTGAATGCAATGCCGTGGTTAAAATTTCTGCATTACAACGAGGGCAACGTGCCCTTTGTCCGCGCTGCCAGAATGTGTTatat  <  1:438433/251‑1 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                            
AAACAAACCGATTTTTAACGGTTTGGCTTCCCCCTCAATAGAAAAACATAGCGGAAATTTTTCCGCTAAATAAGCAATAATCTCTTTATTATTTGTTAACTTTTGTATTTCTGTCATTGCGAAATCCTGCATAGATTTTAATAAGTAGAAAAACTTACCATAGTTTAGCTTACTTTCAGAAAAAATGACAATTAATAATCTATCTACTGAAAAGAAATAATAAATTCCGCGAAAAATGACCGCACTTTTTCTGTCTAAGCAAGATAAATTCGTACTAAAAACGAAAAATACAGTATAAAATACGCGCTTCTTTAATATTTAGGTAACTTGATTCATGACAGCACATTCCAAGTTAAATTTGGCCGACGCTACTTTTCATCGCCTCGTTCGTTGCGGTGAATGCAATGCCGTGGTTAAAATTTCTGCATTACAACGAGGGCAACGTGCCCTTTGTCCGCGCTGCCAGAATGTGTTATAT  >  1/1742647‑1743124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑