Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,741,795	T→C	intergenic (+99437/‑39930)	unknown → / → unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,741,795	0	T	C	100.0%	236.1 / NA	77	intergenic (+99437/‑39930)	unknown/unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (47/30);  total (47/30)

CGCGCGCTTCCCATAACTTATTCGCTTCTTTTTCGGTTTTCGGCCAGGCTGCTTTTTCACGATCAATTTCAATTTGATCATCATCGTTCAAATGCGGTTCTTTATCCAGCAAAGACAAGGCATAACGATAACGTTCATAACGGCGTTTCATCATTAACTCATACATATTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCATCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCCAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGCGGGTTGTCGCACGTTTCGTGGCAAACATGTTGGATTCCGACGGCTGTAAAGAGACCAGATCACTTTGTTTTAATTTCGGTTGTACCGCGTCCGCAATATTGACATTAAGCAAAAAGGAAGTTA  >  1/1741556‑1742038                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                    cgcgcgCTTCCCATAACTTATTCGCTTCTTTTTCGGTTTTCGGCCAGGCTGCTTTTTCACGATCAATTTCAATTTGATCATCATCGTTCAAATGCGGTTCTTTATCCAGCAAAGACAAGGCATAACGATAACGTTCATAACGGCGTTTCATCATTAACTCATACATATTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGAt                                                                                                                                                                                                                                          <  1:55176/251‑1 (MQ=255)         tCCCATAACTTATTCGCTTCTTTTTCGGTTTTCGGCCAGGCTGCTTTTTCACGATCAATTTCAATTTGATCATCATCGTTCAAATGCGGTTCTTTATCCAGCAAAGACAAGGCATAACGATAACGTTCATAACGGCGTTTCATCATTAACTCATACATATTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGAt                                                                                                                                                                                                                                          >  1:841542/1‑243 (MQ=255)         tCCCATAACTTATTCGCTTCTTTTTCGGTTTTCGGCCAGGCTGCTTTTTCACGATCAATTTCAATTTGATCATCATCGTTCAAATGCGGTTCTTTATCCAGCAAAGACAAGGCATAACGATAACGTTCATAACGGCGTTTCATCATTAACTCATACATATTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGAt                                                                                                                                                                                                                                          >  1:501491/1‑243 (MQ=255)         tCCCATAACTTATTCGCTTCTTTTTCGGTTTTCGGCCAGGCTGCTTTTTCACGATCAATTTCAATTTGATCATCATCGTTCAAATGCGGTTCTTTATCCAGCAAAGACAAGGCATAACGATAACGTTCATAACGGCGTTTCATCATTAACTCATACATATTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCaaaaa                                                                                                                                                                                                                                  >  1:221222/1‑251 (MQ=255)         tCCCATAACTTATTCGCTTCTTTTTCGGTTTTCGGCCAGGCTGCTTTTTCACGATCAATTTCAATTTGATCATCATCGTTCAAATGCGGTTCTTTATCCAGCAAAGACAAGGCATAACGATAACGTTCATAACGGCGTTTCATCATTAACTCATACATATTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCaaaaa                                                                                                                                                                                                                                  >  1:36231/1‑251 (MQ=255)                      tCGCTTCTTTTTCGGTTTTCGGCCAGGCTGCTTTTTCACGATCAATTTCAATTTGATCATCATCGTTCAAATGCGGTTCTTTATCCAGCAAAGACAAGGCATAACGATAACGTTCATAACGGCGTTTCATCATTAACTCATACATATTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTa                                                                                                                                                                                                                     <  1:695252/251‑1 (MQ=255)                           tcttTTTCGGTTTTCGGCCAGGCTGCTTTTTCACGATCAATTTCAATTTGATCATCATCGTTCAAATGCGGTTCTTTATCCAGCAAAGACAAGGCATAACGATAACGTTCATAACGGCGTTTCATCATTAACTCATACATATTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATc                                                                                                                                                                                                                <  1:455660/251‑1 (MQ=255)                                    gttttcggCCAGGCTGCTTTTTCACGATCAATTTCAATTTGATCATCATCGTTCAAATGCGGTTCTTTATCCAGCAAAGACAAGGCATAACGATAACGTTCATAACGGCGTTTCATCATTAACTCATACATATTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCtt                                                                                                                                                                                                        <  1:301997/250‑1 (MQ=255)                                                        ttCACGATCAATTTCAATTTGATCATCATCGTTCAAATGCGGTTCTTTATCCAGCAAAGACAAGGCATAACGATAACGTTCATAACGGCGTTTCATCATTAACTCATACATATTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAAt                                                                                                                                                                                     >  1:54663/1‑249 (MQ=255)                                                                  atttcaatttGATCATCATCGTTCAAATGCGGTTCTTTATCCAGCAAAGACAAGGCATAACGATAACGTTCATAACGGCGTTTCATCATTAACTCATACATATTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATc                                                                                                                                                                                                                >  1:535478/1‑212 (MQ=255)                                                                     tcaatttGATCATCATCGTTCAAATGCGGTTCTTTATCCAGCAAAGACAAGGCATAACGATAACGTTCATAACGGCGTTTCATCATTAACTCATACATATTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAgg                                                                                                                                                                                                   >  1:558406/1‑222 (MQ=255)                                                                                    tcGTTCAAATGCGGTTCTTTATCCAGCAAAGACAAGGCATAACGATAACGTTCATAACGGCGTTTCATCATTAACTCATACATATTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGaaa                                                                                                                                                       <  1:626670/251‑1 (MQ=255)                                                                                            aTGCGGTTCTTTATCCAGCAAAGACAAGGCATAACGATAACGTTCATAACGGCGTTTCATCATTAACTCATACATATTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGt                                                                                                                                               <  1:381872/251‑1 (MQ=255)                                                                                                   tCTTTATCCAGCAAAGACAAGGCATAACGATAACGTTCATAACGGCGTTTCATCATTAACTCATACATATTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATgaga                                                                                                                                        >  1:514995/1‑251 (MQ=255)                                                                                                        aTCCAGCAAAGACAAGGCATAACGATAACGTTCATAACGGCGTTTCATCATTAACTCATACATATTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGt                                                                                                                                                                                                  >  1:203461/1‑188 (MQ=255)                                                                                                        aTCCAGCAAAGACAAGGCATAACGATAACGTTCATAACGGCGTTTCATCATTAACTCATACATATTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGt                                                                                                                                               >  1:344101/1‑239 (MQ=255)                                                                                                        aTCCAGCAAAGACAAGGCATAACGATAACGTTCATAACGGCGTTTCATCATTAACTCATACATATTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAg                                                                                                                                   <  1:348527/251‑1 (MQ=255)                                                                                                                 aGACAAGGCATAACGATAACGTTCATAACGGCGTTTCATCATTAACTCATACATATTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGCggg                                                                                                                          >  1:286462/1‑251 (MQ=255)                                                                                                                    cAAGGCATAACGATAACGTTCATAACGGCGTTTCATCATTAACTCATACATATTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTaa                                                                                                                                                                                                >  1:281069/1‑178 (MQ=255)                                                                                                                            aacgataacgTTCATAACGGCGTTTCATCATTAACTCATACATATTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATc                                                                                                                                                     >  1:65686/1‑213 (MQ=255)                                                                                                                             acgataacgTTCATAACGGCGTTTCATCATTAACTCATACATATTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGCGGGTTGTCGCACGtt                                                                                                              >  1:479284/1‑251 (MQ=255)                                                                                                                                        cATAACGGCGTTTCATCATTAACTCATACATATTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGCGGGTTGTCGCACGTTTCGTGGCaaa                                                                                                    >  1:304305/1‑250 (MQ=255)                                                                                                                                                            aaCTCATACATATTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGaaa                                                                                                                                                       <  1:868459/179‑1 (MQ=255)                                                                                                                                                            aaCTCATACATATTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGt                                                                                                                                               <  1:202424/187‑1 (MQ=255)                                                                                                                                                            aaCTCATACATATTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGCGGGTTGTCGCACGTTTCGTGGCAAAc                                                                                                   >  1:660738/1‑231 (MQ=255)                                                                                                                                                            aaCTCATACATATTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGCGGGTTGTCGCACGTTTCGTGGCAAACATGTTGGATTCCGACgg                                                                                  >  1:750680/1‑248 (MQ=255)                                                                                                                                                            aaCTCATACATATTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTACCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAAGCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGAc                                                                                                                                       <  1:325397/195‑1 (MQ=255)                                                                                                                                                              cTCATACATATTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAatat                                                                                                                                                                                                                                               <  1:669131/89‑1 (MQ=255)                                                                                                                                                              cTCATACATATTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGt                                                                                                                                  >  1:414534/1‑198 (MQ=255)                                                                                                                                                              cTCATACATATTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGCGGGTTGTCGCACGTTTCGTGGCAAACATGTTGGATTCCGACGGCTGTa                                                                             >  1:764712/1‑251 (MQ=255)                                                                                                                                                                catacataTTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGt                                                                                                                                               <  1:82336/183‑1 (MQ=255)                                                                                                                                                                catacataTTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGCGGGTTGTCGCACGTTTCGTGGCAAACATGTTGGAt                                                                                          <  1:23615/236‑1 (MQ=255)                                                                                                                                                                catacataTTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGCGGGTTGTCGCACGTTTCGTGGCAAACATGTTGGATTCCGACGGCTGTaaa                                                                           >  1:143221/1‑251 (MQ=255)                                                                                                                                                                  tacataTTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATtc                                                                                                                                                                      >  1:200648/1‑158 (MQ=255)                                                                                                                                                                    cataTTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGCGGGTTGTCGCACGTTTCGTGGCAAACATGTTGGATTCCGACGGCTGTAAAgaga                                                                       >  1:860772/1‑251 (MQ=255)                                                                                                                                                                      tatTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATct                                                                                                                                                                                                               <  1:165119/113‑2 (MQ=255)                                                                                                                                                                      tatTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATc                                                                                                                                                     >  1:373655/1‑171 (MQ=255)                                                                                                                                                                      tatTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGAc                                                                                                                                       <  1:380383/185‑1 (MQ=255)                                                                                                                                                                      tatTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGCGGGTTGTCGCACGTTTCGTGGCAAACATGTTGGATTCCGACGGCTGTAAAGAGAc                                                                      >  1:796357/1‑250 (MQ=255)                                                                                                                                                                      tatTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGCGGGTTGTCGCACGTTTCGTGGCAAACATGTTGGATTCCGACGGCTGTAAAGAGAc                                                                      >  1:178796/1‑250 (MQ=255)                                                                                                                                                                      tatTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGCGGGTTGTCGCACGTTTCGTGGCAAACATGTTGGATTCCGACGGCTGTAAAGAGAc                                                                      >  1:604196/1‑250 (MQ=255)                                                                                                                                                                       atTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATgag                                                                                                                                         <  1:689476/182‑1 (MQ=255)                                                                                                                                                                       atTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGCGGGTTGTCGCACGTTTCGTGGCAAACATGTTGGATTCCGACGGCTGTAAAGAGACCa                                                                    >  1:683754/1‑251 (MQ=255)                                                                                                                                                                              gCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGCGGGTTGTCGCACGTTTCGTGGCAAACATGTTGGATTCCGACGGCTGTAAAGAGACCAGATCa                                                               >  1:779250/1‑249 (MQ=255)                                                                                                                                                                                 atatCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTaaaa                                                                                                                                                                                                                  <  1:585430/99‑1 (MQ=255)                                                                                                                                                                                 atatCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGCGGGTTGTCGCACGTTTCGTGGCAAACATGTTGGATTCCGACGGCTGTAAAGAGACCAGATCACTTTg                                                          >  1:146168/1‑251 (MQ=255)                                                                                                                                                                                  tatCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGaaa                                                                                                                                                       >  1:841003/1‑157 (MQ=255)                                                                                                                                                                                  tatCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGCGGGTTGTCGCACGTTTCGTGGCAAACATGTTGGATTCCGACGGCTGTAAag                                                                          >  1:163615/1‑234 (MQ=255)                                                                                                                                                                                   atCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGc                                                                                                                             >  1:345869/1‑182 (MQ=255)                                                                                                                                                                                   atCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGCGGGTTGTCGCACGTTTCGTGGCAAACATGTTGGATTCCGACGGCTGTAAAGAGACCAGATCACTTTGtt                                                        >  1:627393/1‑251 (MQ=255)                                                                                                                                                                                     cTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGCGGGTTGTCGCACGTTTCGTGGCAAACATGTTGGATTCCGACGGCTGTAAAGAGACCAGATCACTTTGtttt                                                      >  1:407924/1‑251 (MQ=255)                                                                                                                                                                                     cTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGCGGGTTGTCGCACGTTTCGTGGCAAACATGTTGGATTCCGACGGCTGTAAAGAGACCAGATCACTTTGtttt                                                      >  1:639420/1‑251 (MQ=255)                                                                                                                                                                                     cTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGCGGGTTGTCGCACGTTTCGTGGCAAACATGTTGGATTCCGACGGCTGTAAAGAGACCAGATCACTTTGtttt                                                      >  1:856213/1‑251 (MQ=255)                                                                                                                                                                                         aTTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGCGGGTTGTCGCACGTTTCGTGGCAAACATGTTGGATTCCGACGGCTGTAAAGAGACCAGATCACTTTGTTTTAAtt                                                  <  1:608738/251‑1 (MQ=255)                                                                                                                                                                                         aTTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGCGGGTTGTCGCACGTTTCGTGGCAAACATGTTGGATTCCGACGGCTGTAAAGAGACCAGATCACTTTGTTTTAAtt                                                  <  1:63984/251‑1 (MQ=255)                                                                                                                                                                                          ttACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGCGGGTTGTCGCACGTTTCGTGGCAAACATGTTGGATTCCGACGGCTGTAAAGAGACCAGATCACTTTGTTTTAAttt                                                 <  1:386248/251‑1 (MQ=255)                                                                                                                                                                                             ccTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGCGGGTTGTCGCACGTTTCGTGGCAAACATGTTGGATTCCGACGGCTGTAAAGAGACCAGATCACTTTGTTTTAATTTCgg                                              >  1:142463/1‑251 (MQ=255)                                                                                                                                                                                                gCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGt                                                                                                                                                                                                  >  1:850422/1‑100 (MQ=255)                                                                                                                                                                                                gCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGc                                                                                                                             >  1:763801/1‑169 (MQ=255)                                                                                                                                                                                                gCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGCGGGTTGTCGCACGTTTCGTGGCAAACATGTTGGATTCCGACGGCTGTAAag                                                                          <  1:518391/220‑1 (MQ=255)                                                                                                                                                                                                   ttCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGCGGGTTGTCGCACGTTTCGTGGCAAACATGTTGGATTCCGACGGCTGTAAAGAGACCAGATCACTTTGTTTTAATTTCGGTTGTAc                                        <  1:676549/251‑1 (MQ=255)                                                                                                                                                                                                     cAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGCGGGTTGTCGCACGTTTCGTGGCAAACATGTTGGATTCCGACGGCTGTAAAGAGACCAGATCACTTTGTTTTAATTTCGGTTGTACcg                                      <  1:421833/251‑1 (MQ=255)                                                                                                                                                                                                            tcatcaATTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGCGGGTTGTCGCACGTTTCGTGGCAAACATGTTGGATTCCGACGGCTGTAAAGAGACCAGATCACTTTGTTTTAATTTCGGTTGTACCGCGTCCg                                >  1:381441/1‑250 (MQ=255)                                                                                                                                                                                                                  aTTTTATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGCGGGTTGTCGCACGTTTCGTGGc                                                                                                       <  1:321662/173‑1 (MQ=255)                                                                                                                                                                                                                    tttATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGCGGGTTGTCGCACGTTTCGTGGCAAACATGTTGGATTCCGACGGCTGTAAAGAGACCAGATCACTTTGTTTTAATTTCGGTTGTACCGCGTCCGCAATATTGa                       <  1:110234/251‑1 (MQ=255)                                                                                                                                                                                                                     ttATTGCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGAc                                                                                                                                       <  1:91908/138‑1 (MQ=255)                                                                                                                                                                                                                          gCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGCGGGTTGTCGCACGTTTCGTGGCAAACATGTTGGATTCCGACGGCTGTAAAGAGACCAGATCACTTTGTTTTAATTTCGGTTGTACCGCGTCCGCAat                            >  1:399588/1‑240 (MQ=255)                                                                                                                                                                                                                          gCCATATTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGCGGGTTGTCGCACGTTTCGTGGCAAACATGTTGGATTCCGACGGCTGTAAAGAGACCAGATCACTTTGTTTTAATTTCGGTTGTACCGCGTCCGCAATATTGACATTaa                 >  1:304602/1‑251 (MQ=255)                                                                                                                                                                                                                               atTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATg                                                                                                                                           <  1:579781/124‑1 (MQ=255)                                                                                                                                                                                                                               atTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGCGGGTTGTCGCACGTTTCGTgg                                                                                                        <  1:668712/159‑1 (MQ=255)                                                                                                                                                                                                                               atTTAGTGAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGCGGGTTGTCGCACGTTTCGTGGCAAACATGTTGGATTCCGACGGCTGTAAAGAGACCAGATCACTTTGTTTTAATTTCGGTTGTACCGCGTCCGCAATATTGACATTAAGCaaa            >  1:648502/1‑251 (MQ=255)                                                                                                                                                                                                                                    gTGATCATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCttt                                                                                                                                                                                 <  1:607661/81‑1 (MQ=255)                                                                                                                                                                                                                                      gAACATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGCGGGTTGTCGCACGTTTCGTGGCAAACATGTTGGATTCCGACGGCTGTAAAGAGACCAGATCACTTTGTTTTAATTTCGGTTGTACCGCGTCCGCAATATTGACATTAAGCAAAAAGGAAg     >  1:113721/1‑251 (MQ=255)                                                                                                                                                                                                                                        aCATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGCGGGTTGTCGCACGTTTCGTGGCAAACATGTTGGATTCCGACGGCTGTAAAGAGACCAGATCACTTTGTTTTAATTTCGGTTGTACCGCGTCCGCAATATTGACATTAAGCAAAAAGGAAGtt   >  1:295163/1‑251 (MQ=255)                                                                                                                                                                                                                                         cATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGCggg                                                                                                                          <  1:87834/131‑1 (MQ=255)                                                                                                                                                                                                                                         cATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGCGGGTTGTCGCACGTTTCGTGGCAAACATGTTGGATTCCGACGGCTGTAAAGAGACCAGATCACTTTGTTTTAATTTCGGTTGTACCGCGTCCGCAATATTGACa                     >  1:149874/1‑232 (MQ=255)                                                                                                                                                                                                                                         cATTTCACCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCTAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGCGGGTTGTCGCACGTTTCGTGGCAAACATGTTGGATTCCGACGGCTGTAAAGAGACCAGATCACTTTGTTTTAATTTCGGTTGTACCGCGTCCGCAATATTGACATTAAGCAAAAAGGAAGTTa  >  1:392501/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                    CGCGCGCTTCCCATAACTTATTCGCTTCTTTTTCGGTTTTCGGCCAGGCTGCTTTTTCACGATCAATTTCAATTTGATCATCATCGTTCAAATGCGGTTCTTTATCCAGCAAAGACAAGGCATAACGATAACGTTCATAACGGCGTTTCATCATTAACTCATACATATTAAAAGCAATATCTAAATTACCTGCATTCAACTCGTCATCAATTTTATTGCCATATTTAGTGAACATTTCATCAATATCGGATTGCAAAAACGTCGTACGGTTAAAATCCAACGCTTTAAGGTAACGATCAAAAATCTTTTTCGAAAATTCATCATCCAGCTGAAATCTACGGTAATGAGACTGAGTTAGGCGGGTTGTCGCACGTTTCGTGGCAAACATGTTGGATTCCGACGGCTGTAAAGAGACCAGATCACTTTGTTTTAATTTCGGTTGTACCGCGTCCGCAATATTGACATTAAGCAAAAAGGAAGTTA  >  1/1741556‑1742038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑