Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,702,094	G→A	intergenic (+59736/‑79631)	unknown → / → unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,702,094	0	G	A	100.0%	208.8 / NA	72	intergenic (+59736/‑79631)	unknown/unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/39);  total (33/39)

CGGTGATAAAACGGGAAGGCAAATGACGTTCTTCCTTGCCATATAAACGGCGGCTTTCCGCATAAGAAATGGTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCGACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTC  >  1/1701853‑1702339                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      cGGTGATAAAACGGGAAGGCAAATGACGTTCTTCCTTGCCATATAAACGGCGGCTTTCCGCATAAGAAATGGTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGcc                                                                                                                                                                                                                                              <  1:95321/251‑1 (MQ=255)      aTAAAACGGGAAGGCAAATGACGTTCTTCCTTGCCATATAAACGGCGGCTTTCCGCATAAGAAATGGTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGAtg                                                                                                                                                                                                                                         <  1:782748/251‑1 (MQ=255)       tAAAACGGGAAGGCAAATGACGTTCTTCCTTGCCATATAAACGGCGGCTTTCCGCATAAGAAATGGTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGAtgt                                                                                                                                                                                                                                        <  1:849613/251‑1 (MQ=255)       tAAAACGGGAAGGCAAATGACGTTCTTCCTTGCCATATAAACGGCGGCTTTCCGCATAAGAAATGGTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGAtgt                                                                                                                                                                                                                                        <  1:170201/251‑1 (MQ=255)         aaaCGGGAAGGCAAATGACGTTCTTCCTTGCCATATAAACGGCGGCTTTCCGCATAAGAAATGGTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGAtg                                                                                                                                                                                                                                         <  1:693522/248‑1 (MQ=255)            cGGGAAGGCAAATGACGTTCTTCCTTGCCATATAAACGGCGGCTTTCCGCATAAGAAATGGTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGgcg                                                                                                                                                                                                                                    >  1:484984/1‑250 (MQ=255)                     aaaTGACGTTCTTCCTTGCCATATAAACGGCGGCTTTCCGCATAAGAAATGGTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGctc                                                                                                                                                                                                                          <  1:92916/251‑1 (MQ=255)                                      gCCATATAAACGGCGGCTTTCCGCATAAGAAATGGTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATg                                                                                                                                                                                                         >  1:172883/1‑251 (MQ=255)                                      gCCATATAAACGGCGGCTTTCCGCATAAGAAATGGTGAGTTTCTGCTTGGCGCGGGGAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATg                                                                                                                                                                                                         >  1:259658/1‑251 (MQ=255)                                       ccATATAAACGGCGGCTTTCCGCATAAGAAATGGTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGa                                                                                                                                                                                                        <  1:354976/251‑1 (MQ=255)                                       ccATATAAACGGCGGCTTTCCGCATAAGAAATGGTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGa                                                                                                                                                                                                        <  1:607150/251‑1 (MQ=255)                                       ccATATAAACGGCGGCTTTCCGCATAAGAAATGGTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGa                                                                                                                                                                                                        <  1:610093/251‑1 (MQ=255)                                         atatAAACGGCGGCTTTCCGCATAAGAAATGGTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTcc                                                                                                                                                                                                             >  1:219619/1‑244 (MQ=255)                                         atatAAACGGCGGCTTTCCGCATAAGAAATGGTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAg                                                                                                                                                                                                      <  1:602238/251‑1 (MQ=255)                                         atatAAACGGCGGCTTTCCGCATAAGAAATGGTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAg                                                                                                                                                                                                      <  1:620010/251‑1 (MQ=255)                                          tataAACGGCGGCTTTCCGCATAAGAAATGGTGAGTTTCTGCTTGGCGCGGGTAATCACGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAg                                                                                                                                                                                                      >  1:510239/1‑250 (MQ=255)                                           ataAACGGCGGCTTTCCGCATAAGAAATGGTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCg                                                                                                                                                                                                    <  1:557758/251‑1 (MQ=255)                                                          ccGCATAAGAAATGGTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGc                                                                                                                                                                                      >  1:810823/1‑250 (MQ=255)                                                              aTAAGAAATGGTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCa                                                                                                                                                                                 <  1:111856/251‑1 (MQ=255)                                                                   aaaTGGTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATc                                                                                                                                                                             <  1:614844/250‑1 (MQ=255)                                                                     aTGGTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGt                                                                                                                                                                          >  1:493865/1‑251 (MQ=255)                                                                        gTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCa                                                                                                                                                                        <  1:717174/250‑1 (MQ=255)                                                                           aGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCatat                                                                                                                                                                     >  1:134020/1‑250 (MQ=255)                                                                            gTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGAtgt                                                                                                                                                                                                                                        <  1:306649/182‑1 (MQ=255)                                                                             tttCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCtt                                                                                                                                                                  >  1:404616/1‑251 (MQ=255)                                                                             tttCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATAATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATc                                                                                                                                                                    <  1:196055/249‑1 (MQ=255)                                                                                             gTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGtt                                                                                                                                                   <  1:95355/250‑1 (MQ=255)                                                                                             gTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGtt                                                                                                                                                   <  1:880498/250‑1 (MQ=255)                                                                                                aTCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGAtg                                                                                                                                                                                                                                         >  1:328285/1‑161 (MQ=255)                                                                                                      aCGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAAtt                                                                                                                                          >  1:20983/1‑250 (MQ=255)                                                                                                      aCGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTc                                                                                                                                         <  1:894966/251‑1 (MQ=255)                                                                                                         tATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACg                                                                                                                                      >  1:532592/1‑251 (MQ=255)                                                                                                                       ctctTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGt                                                                                                                                                                          >  1:103931/1‑201 (MQ=255)                                                                                                                       ctctTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACAcc                                                                                                                         <  1:852175/250‑1 (MQ=255)                                                                                                                       ctctTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCa                                                                                                                        >  1:227467/1‑251 (MQ=255)                                                                                                                       ctctTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATAAAGCGCGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCa                                                                                                                        >  1:434712/1‑251 (MQ=255)                                                                                                                           tcttcCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATg                                                                                                                                                                                                         >  1:38144/1‑166 (MQ=255)                                                                                                                                   aCGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGt                                                                                                            <  1:571353/251‑1 (MQ=255)                                                                                                                                              cttcAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAGATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACg                                                                                                  >  1:232659/1‑250 (MQ=255)                                                                                                                                                       aaCGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCGTCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTcccc                                                                                         <  1:194663/250‑1 (MQ=255)                                                                                                                                                        aCGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCtt                                                                                       <  1:344721/251‑1 (MQ=255)                                                                                                                                                                tCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGccc                                                                               <  1:577202/251‑1 (MQ=255)                                                                                                                                                                tCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGccc                                                                               <  1:753392/251‑1 (MQ=255)                                                                                                                                                                 cGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCaa                                                                                                      >  1:396035/1‑227 (MQ=255)                                                                                                                                                                          tCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATc                                                                                                                                                                             >  1:703898/1‑147 (MQ=255)                                                                                                                                                                          tCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAAttc                                                                                                                    >  1:388727/1‑204 (MQ=255)                                                                                                                                                                          tCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGtt                                                                     <  1:848241/251‑1 (MQ=255)                                                                                                                                                                              tcttcCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATc                                                                                                                                                                             >  1:613666/1‑143 (MQ=255)                                                                                                                                                                                ttcCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCTGACACCAAttc                                                                                                                    <  1:235705/198‑1 (MQ=255)                                                                                                                                                                                    ccGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTg                                                           <  1:173154/250‑1 (MQ=255)                                                                                                                                                                                        cAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATAc                                                       <  1:853839/251‑1 (MQ=255)                                                                                                                                                                                         aaTCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGtt                                                                                                                                                   >  1:703286/1‑158 (MQ=255)                                                                                                                                                                                             aTGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAAccc                                                  <  1:792026/251‑1 (MQ=255)                                                                                                                                                                                                  aaCACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAAt                                             <  1:769372/251‑1 (MQ=255)                                                                                                                                                                                                           aaaCTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATca                                    <  1:730571/251‑1 (MQ=255)                                                                                                                                                                                                              cTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTcgcc                                                                                                                                                                                                                    <  1:198601/72‑1 (MQ=255)                                                                                                                                                                                                              cTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATc                                                                                                                                                                             <  1:428690/111‑1 (MQ=255)                                                                                                                                                                                                              cTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACaa                                 >  1:702108/1‑251 (MQ=255)                                                                                                                                                                                                              cTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACaa                                 >  1:21712/1‑251 (MQ=255)                                                                                                                                                                                                                  aaGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATc                             <  1:314693/251‑1 (MQ=255)                                                                                                                                                                                                                    gCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATAc                                                       >  1:632251/1‑223 (MQ=255)                                                                                                                                                                                                                    gCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAAccc                                                  >  1:622001/1‑228 (MQ=255)                                                                                                                                                                                                                    gCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACaaaa                               <  1:121768/247‑1 (MQ=255)                                                                                                                                                                                                                    gCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCg                           >  1:539257/1‑251 (MQ=255)                                                                                                                                                                                                                           aCCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTa                                        >  1:577582/1‑231 (MQ=255)                                                                                                                                                                                                                           aCCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTgcg                     >  1:660590/1‑250 (MQ=255)                                                                                                                                                                                                                           aCCGAATGCAGCATCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGCCAGATCCGACATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTgcg                     >  1:78286/1‑250 (MQ=255)                                                                                                                                                                                                                                     gCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATAc                                                       <  1:699673/206‑1 (MQ=255)                                                                                                                                                                                                                                       gTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTc                                                                                                                                                           >  1:502665/1‑104 (MQ=255)                                                                                                                                                                                                                                             atTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATttcttc  <  1:281614/251‑1 (MQ=255)                                                                                                                                                                                                                                              tttCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATttcttc  >  1:792460/1‑250 (MQ=255)                                                                                                                                                                                                                                              tttCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATttcttc  >  1:235853/1‑250 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      CGGTGATAAAACGGGAAGGCAAATGACGTTCTTCCTTGCCATATAAACGGCGGCTTTCCGCATAAGAAATGGTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCGACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTC  >  1/1701853‑1702339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑