Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,696,984	A→C	intergenic (+54626/‑84741)	unknown → / → unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,696,984	0	A	C	100.0%	177.6 / NA	62	intergenic (+54626/‑84741)	unknown/unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (38/24);  total (38/24)

CCATTGTGATGGGCGTGGTGATTTTGTTTTCGTTTTTCAACGGCACTATGACGGCAACCACCCAGCGTTTTATTAACGTGGAAAAAGCCTCAAACGACTCAAGCAGAGTAAATCAGGTTTTTAACATCAGCATCTTGAATCACCTGTTTATTATGCTGGTCGTGGTGATTCTGGCGGAAACGGCAGGATTATGGTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAAAATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGATCGTGGCGCATGAGCGAATGTCCTTTTATGCCTGGCTCGGGTTGGCGGAAACCGGCTTAAAACTGGGCGCAATTTTTATTCTCACGCAAATAGAAAACCACGATAAATTGATTTCTTACAGTTTACTGCTGCTGTGCGTAAGTTTATTGGTTTTCACCCTGTATTTTCGTTTCACCCG  >  1/1696740‑1697229                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      ccATTGTGATGGGCGTGGTGATTTTGTTTTCGTTTTTCAACGGCACTATGACGGCAACCACCCAGCGTTTTATTAACGTGGAAAAAGCCTCAAACGACTCAAGCAGAGTAAATCAGGTTTTTAACATCAGCATCTTGAATCACCTGTTTATTATGCTGGTCGTGGTGATTCTGGCGGAAACGGCAGGATTATGGTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATtgtg                                                                                                                                                                                                                                                 >  1:660158/1‑251 (MQ=255) ccATTGTGATGGGCGTGGTGATTTTGTTTTCGTTTTTCAACGGCACTATGACGGCAACCACCCAGCGTTTTATTAACGTGGAAAAAGCCTCAAACGACTCAAGCAGAGTAAATCAGGTTTTTAACATCAGCATCTTGAATCACCTGTTTATTATGCTGGTCGTGGTGATTCTGGCGGAAACGGCAGGATTATGGTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATtgtg                                                                                                                                                                                                                                                 >  1:203144/1‑251 (MQ=255)   aTTGTGATGGGCGTGGTGATTTTGTTTTCGTTTTTCAACGGCACTATGACGGCAACCACCCAGCGTTTTATTAACGTGGAAAAAGCCTCAAACGACTCAAGCAGAGTAAATCAGGTTTTTAACATCAGCATCTTGAATCACCTGTTTATTATGCTGGTCGTGGTGATTCTGGCGGAAACGGCAGGATTATGGTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTa                                                                                                                                                                                                                                               >  1:447933/1‑251 (MQ=255)   aTTGTGATGGGCGTGGTGATTTTGTTTTCGTTTTTCAACGGCACTATGACGGCAACCACCCAGCGTTTTATTAACGTGGAAAAAGCCTCAAACGACTCAAGCAGAGTAAATCAGGTTTTTAACATCAGCATCTTGAATCACCTGTTTATTATGCTGGTCGTGGTGATTCTGGCGGAAACGGCAGGATTATGGTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTa                                                                                                                                                                                                                                               >  1:309734/1‑251 (MQ=255)    ttGTGATGGGCGTGGTGATTTTGTTTTCGTTTTTCAACGGCACTATGACGGCAACCACCCAGCGTTTTATTAACGTGGAAAAAGCCTCAAACGACTCAAGCAGAGTAAATCAGGTTTTTAACATCAGCATCTTGAATCACCTGTTTATTATGCTGGTCGTGGTGATTCTGGCGGAAACGGCAGGATTATGGTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTAc                                                                                                                                                                                                                                              <  1:769136/251‑1 (MQ=255)      gtgATGGGCGTGGTGATTTTGTTTTCGTTTTTCAACGGCACTATGACGGCAACCACCCAGCGTTTTATTAACGTGGAAAAAGCCTCAAACGACTCAAGCAGAGTAAATCAGGTTTTTAACATCAGCATCTTGAATCACCTGTTTATTATGCTGGTCGTGGTGATTCTGGCGGAAACGGCAGGATTATGGTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTACCa                                                                                                                                                                                                                                            >  1:522365/1‑251 (MQ=255)      gtgATGGGCGTGGTGATTTTGTTTTCGTTTTTCAACGGCACTATGACGGCAACCACCCAGCGTTTTATTAACGTGGAAAAAGCCTCAAACGACTCAAGCAGAGTAAATCAGGTTTTTAACATCAGCATCTTGAATCACCTGTTTATTATGCTGGTCGTGGTGATTCTGGCGGAAACGGCAGGATTATGGTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTACCa                                                                                                                                                                                                                                            >  1:91972/1‑251 (MQ=255)                   tgATTTTGTTTTCGTTTTTCAACGGCACTATGACGGCAACCACCCAGCGTTTTATTAACGTGGAAAAAGCCTCAAACGACTCAAGCAGAGTAAATCAGGTTTTTAACATCAGCATCTTGAATCACCTGTTTATTATGCTGGTCGTGGTGATTCTGGCGGAAACGGCAGGATTATGGTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTc                                                                                                                                                                                                                               <  1:858254/251‑1 (MQ=255)                    gATTTTGTTTTCGTTTTTCAACGGCACTATGACGGCAACCACCCAGCGTTTTATTAACGTGGAAAAAGCCTCAAACGACTCAAGCAGAGTAAATCAGGTTTTTAACATCAGCATCTTGAATCACCTGTTTATTATGCTGGTCGTGGTGATTCTGGCGGAAACGGCAGGATTATGGTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTACCAGGTAGCAt                                                                                                                                                                                                                                    >  1:259728/1‑245 (MQ=255)                                           gCACTATGACGGCAACCACCCAGCGTTTTATTAACGTGGAAAAAGCCTCAAACGACTCAAGCAGAGTAAATCAGGTTTTTAACATCAGCATCTTGAATCACCTGTTTATTATGCTGGTCGTGGTGATTCTGGCGGAAACGGCAGGATTATGGTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTACCAGGt                                                                                                                                                                                                                                         <  1:96164/217‑1 (MQ=255)                                                           caccCAGCGTTTTATTAACGTGGAAAAAGCCTCAAACGACTCAAGCAGAGTAAATCAGGTTTTTAACATCAGCATCTTGAATCACCTGTTTATTATGCTGGTCGTGGTGATTCTGGCGGAAACGGCAGGATTATGGTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTa                                                                                                                                                                                       >  1:713151/1‑251 (MQ=255)                                                                 gCGTTTTATTAACGTGGAAAAAGCCTCAAACGACTCAAGCAGAGTAAATCAGGTTTTTAACATCAGCATCTTGAATCACCTGTTTATTATGCTGGTCGTGGTGATTCTGGCGGAAACGGCAGGATTATGGTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGATCg                                                                                                                                                                                 >  1:395904/1‑251 (MQ=255)                                                                 gCGTTTTATTAACGTGGAAAAAGCCTCAAACGACTCAAGCAGAGTAAATCAGGTTTTTAACATCAGCATCTTGAATCACCTGTTTATTATGCTGGTCGTGGTGATTCTGGCGGAAACGGCAGGATTATGGTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGATCg                                                                                                                                                                                 >  1:217583/1‑251 (MQ=255)                                                                 gCGTTTTATTAACGTGGAAAAAGCCTCAAACGACTCAAGCAGAGTAAATCAGGTTTTTAACATCAGCATCTTGAATCACCTGTTTATTATGCTGGTCGTGGTGATTCTGGCGGAAACGGCAGGATTATGGTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGATCg                                                                                                                                                                                 >  1:659279/1‑251 (MQ=255)                                                                  cGTTTTATTAACGTGGAAAAAGCCTCAAACGACTCAAGCAGAGTAAATCAGGTTTTTAACATCAGCATCTTGAATCACCTGTTTATTATGCTGGTCGTGGTGATTCTGGCGGAAACGGCAGGATTATGGTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGAt                                                                                                                                                                                   <  1:846497/248‑1 (MQ=255)                                                                  cGTTTTATTAACGTGGAAAAAGCCTCAAACGACTCAAGCAGAGTAAATCAGGTTTTTAACATCAGCATCTTGAATCACCTGTTTATTATGCTGGTCGTGGTGATTCTGGCGGAAACGGCAGGATTATGGTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGATCg                                                                                                                                                                                 <  1:429155/250‑1 (MQ=255)                                                                    ttttATTAACGTGGAAAAAGCCTCAAACGACTCAAGCAGAGTAAATCAGGTTTTTAACATCAGCATCTTGAATCACCTGTTTATTATGCTGGTCGTGGTGATTCTGGCGGAAACGGCAGGATTATGGTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGAt                                                                                                                                                                                   <  1:213740/246‑1 (MQ=255)                                                                      ttattaACGTGGAAAAAGCCTCAAACGACTCAAGCAGAGTAAATCAGGTTTTTAACATCAGCATCTTGAATCACCTGTTTATTATGCTGGTCGTGGTGATTCTGGCGGAAACGGCAGGATTATGGTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATc                                                                                                                                                                                                             >  1:179108/1‑218 (MQ=255)                                                                            aCGTGGAAAAAGCCTCAAACGACTCAAGCAGAGTAAATCAGGTTTTTAACATCAGCATCTTGAATCACCTGTTTATTATGCTGGTCGTGGTGATTCTGGCGGAAACGGCAGGATTATGGTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGATCGTGGCGCATGAg                                                                                                                                                                      <  1:185376/251‑1 (MQ=255)                                                                                                gACTCAAGCAGAGTAAATCAGGTTTTTAACATCAGCATCTTGAATCACCTGTTTATTATGCTGGTCGTGGTGATTCTGGCGGAAACGGCAGGATTATGGTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAg                                                                                                                                                                                                      <  1:692792/199‑1 (MQ=255)                                                                                                gACTCAAGCAGAGTAAATCAGGTTTTTAACATCAGCATCTTGAATCACCTGTTTATTATGCTGGTCGTGGTGATTCTGGCGGAAACGGCAGGATTATGGTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGt                                                                                                                                                                                                     <  1:136548/200‑1 (MQ=255)                                                                                                       gCAGAGTAAATCAGGTTTTTAACATCAGCATCTTGAATCACCTGTTTATTATGCTGGTCGTGGTGATTCTGGCGGAAACGGCAGGATTATGGTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTAcc                                                                                                                                                                                                                                             <  1:783006/153‑1 (MQ=255)                                                                                                             tAAATCAGGTTTTTAACATCAGCATCTTGAATCACCTGTTTATTATGCTGGTCGTGGTGATTCTGGCGGAAACGGCAGGATTATGGTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGATCGTGGCGCATGAGCGAATGTCCTTTTATGCCTGGCTCGGGTTGGCg                                                                                                                                     <  1:265526/251‑1 (MQ=255)                                                                                                                                    aTCTTGAATCACCTGTTTATTATGCTGGTCGTGGTGATTCTGGCGGAAACGGCAGGATTATGGTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTAc                                                                                                                                                                                                                                              >  1:328904/1‑123 (MQ=255)                                                                                                                                         gAATCACCTGTTTATTATGCTGGTCGTGGTGATTCTGGCGGAAACGGCAGGATTATGGTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTAccc                                                                                                                                                                                                 >  1:621750/1‑163 (MQ=255)                                                                                                                                             cACCTGTTTATTATGCTGGTCGTGGTGATTCTGGCGGAAACGGCAGGATTATGGTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGATCGTGGCGCATGAGCGAATGTCCTTTTATGCCTGGCTCGGGTTGGCGGAAACCGGCTTAAAACTGGGCGCAATTTTTAt                                                                                                     >  1:564576/1‑251 (MQ=255)                                                                                                                                               ccTGTTTATTATGCTGGTCGTGGTGATTCTGGCGGAAACGGCAGGATTATGGTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTgtgt                                                                                                                                                                                                                                                >  1:99737/1‑110 (MQ=255)                                                                                                                                                 tGTTTATTATGCTGGTCGTGGTGATTCTGGCGGAAACGGCAGGATTATGGTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGATCGTGGCGCATGAGCGAATGTCCTTTTATGCCTGGCTCGGGTTGGCGGAAACCGGCTTAAAACTGGGCGCAATTTTTATtct                                                                                                  >  1:103135/1‑250 (MQ=255)                                                                                                                                                  gTTTATTATGCTGGTCGTGGTGATTCTGGCGGAAACGGCAGGATTATGGTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGATCGTGGCGCATGAGCGAATGTCCTTTTATGCCTGGCTCGGGTTGGCGGAAACCGGCTTAAAACTGGGCGCAATTTTTATTCTCa                                                                                                >  1:464187/1‑251 (MQ=255)                                                                                                                                                    ttattaTGCTGGTCGTGGTGATTCTGGCGGAAACGGCAGGATTATGGTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTTTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGATCGTGGCGCATGAGCGAATGTCCTTTTATGCCTGGCTCGGGTTGGCGGAAACCGGCTTAAAACTGGGCGCAATTTTTATTCTCACg                                                                                              >  1:631965/1‑251 (MQ=255)                                                                                                                                                    ttattaTGCTGGTCGTGGTGATTCTGGCGGAAACGGCAGGATTATGGTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGAt                                                                                                                                                                                   <  1:464809/166‑1 (MQ=255)                                                                                                                                                    ttattaTGCTGGTCGTGGTGATTCTGGCGGAAACGGCAGGATTATGGTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGATCGTGGCGCATGAGCGAATGTCCTTTTATGcc                                                                                                                                                    >  1:498578/1‑197 (MQ=255)                                                                                                                                                    ttattaTGCTGGTCGTGGTGATTCTGGCGGAAACGGCAGGATTATGGTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGATCGTGGCGCATGAGCGAATGTCCTTTTATGCCTGGCTCGGGTTGGCGGAAACCGGCTTAAAAc                                                                                                                     >  1:850279/1‑228 (MQ=255)                                                                                                                                                    ttattaTGCTGGTCGTGGTGATTCTGGCGGAAACGGCAGGATTATGGTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGATCGTGGCGCATGAGCGAATGTCCTTTTATGCCTGGCTCGGGTTGGCGGAAACCGGCTTAAAACTGGGCGCAATTTTTAtt                                                                                                    >  1:235328/1‑245 (MQ=255)                                                                                                                                                                                       gCAGGATTATGGTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGATCGTGGCGCATGAGCGAATGTCCTTTTATGc                                                                                                                                                     <  1:702859/161‑1 (MQ=255)                                                                                                                                                                                        cAGGATTATGGTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGATCGTGGCGCATGAGCGAATGTCCTTTTATGCCTGGCTCGGGTTGGCGGAAACCGGCTTAAAACTGGGCGCAATTTTTATTCTCACGCAAATAGAAAACCACGATAAATTGATTTCTTACAg                                                           <  1:233028/250‑1 (MQ=255)                                                                                                                                                                                          ggATTATGGTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGATCGTGGCGCATGAGCGAATGTCCTTTTATGCCTGGCTCGGGTTGGCGGAAACCGGCTTAAAACTGGGCGCAATTTTTATTCTCACGCAAATAGAAAAc                                                                                  <  1:566657/225‑1 (MQ=255)                                                                                                                                                                                          ggATTATGGTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGATCGTGGCGCATGAGCGAATGTCCTTTTATGCCTGGCTCGGGTTGGCGGAAACCGGCTTAAAACTGGGCGCAATTTTTATTCTCACGCAAATAGAAAACCACGATAAATTGATTTCTTACAGttt                                                        >  1:512083/1‑251 (MQ=255)                                                                                                                                                                                            aTTATGGTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGATCGTGGCGCATGAGCGAATGTCCTTTTATGCCTGGCTCGGGTTGGCGGAAACCGGCTTAAAACTGGGCGCAATTTTTATTCTCACGCAAATAGAAAACCACGATAAATTGATTTCTTACAGTTTAc                                                      <  1:127308/251‑1 (MQ=255)                                                                                                                                                                                               aTGGTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGATCGTGGCGCATGAGCGAATGTCCTTTTATGCCTGGCTCGGGTTGGCGGAAACCGGCTTAAAACTGGGCGCAATTTTTATTCTCACGCAAATAGAAAACCACGATAAATTGATTTCTTACAGTTTActgc                                                   <  1:375920/251‑1 (MQ=255)                                                                                                                                                                                                  gTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGATCGTGGCGCATGAGCGAATGTCCTTTTATGCCTGGCTCGGGTTGGCGGAAACCGGCTTAAAACTGGGCGCAATTTTTATtc                                                                                                   <  1:40654/200‑1 (MQ=255)                                                                                                                                                                                                  gTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGATCGTGGCGCATGAGCGAATGTCCTTTTATGCCTGGCTCGGGTTGGCGGAAACCGGCTTAAAACTGGGCGCAATTTTTATTCTCACGCAAATAGAAAACCACGATAAAt                                                                        >  1:277510/1‑227 (MQ=255)                                                                                                                                                                                                            cATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGATCGTGGCGCATGAGCGAATGTCCTTTTATGCCTGGCTCGGGTTGGCGGAAACCGGCTTAAAACTGGGCGCAATTTTTATTCTCACGCAAATAGAAAACCACGATAAATTGATTTCTTACAGTTTACTGCTGCTGTGCGTAAg                                      <  1:327482/251‑1 (MQ=255)                                                                                                                                                                                                            cATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGATCGTGGCGCATGAGCGAATGTCCTTTTATGCCTGGCTCGGGTTGGCGGAAACCGGCTTAAAACTGGGCGCAATTTTTATTCTCACGCAAATAGAAAACCACGATAAATTGATTTCTTACAGTTTACTGCTGCTGTGCGTAAg                                      >  1:130266/1‑251 (MQ=255)                                                                                                                                                                                                               aaaTTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACg                                                                                                                                                                                          >  1:641956/1‑100 (MQ=255)                                                                                                                                                                                                               aaaTTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGATCGTGGCGCATGAGCGAATGTCCTTTTATGCCTGGCTCGGGTTGGCGGAAACCGGCTTAAAACTGGGCGCAATTTTTATTCTCACGCAAATAGAAAACCACGATAAATTGATTTCTTACAGTTTActgctgctg                                              <  1:830963/240‑1 (MQ=255)                                                                                                                                                                                                                aaTTGAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGATCGTGGCGCATGAGCGAATGTCCTTTTATGCCTGGCTCGGGTTGGCGGAAACCGGCTTAAAACTGGGCGCAATTTTTATTCTCACGCAAATAGAAAACCACGATAAATTGATTTCTTACAGTTTACTGCTGCTGTGCGTAAGTTTa                                  >  1:420574/1‑251 (MQ=255)                                                                                                                                                                                                                    taagctacccgccccgcGTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGATCGTGGCGCATGAGCGAATGTCCTTTTATGCCTGGCTCGGGTTGGCGGAAACCGGCTTAAAACTGGGCGCAATTTTTATTCTCACGCAAATAGAAAACCACGATAAATTGATTTCTTACAGTTTACTGCTGCTGTGCGTAAGTTTATTgg                              <  1:190915/244‑1 (MQ=255)                                                                                                                                                                                                                    gAACATTCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGATCGTGGCGCATGAGCGAATGTCCTTTTATGCCTGGCTCGGGTTGGCGGAAACCGGCTTAAAACTGGGCGCAATTTTTATTCTCACGCAAATAGAAAACCACGATAAATTGATTTCTTACAGTTTActgc                                                   <  1:74838/230‑1 (MQ=255)                                                                                                                                                                                                                         ttCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGATCGTGGCGCATGAGc                                                                                                                                                                     >  1:96280/1‑111 (MQ=255)                                                                                                                                                                                                                         ttCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGATCGTGGCGCATGAGCGAATGTCCTTTTATGCCTGGCTCGGGTTGGCGGAAACCGGCTTAAAACTGGGCGCAATTTTTATTCTCACGCAAATAGAAAACCACGATAAATTGATTTCTTACAGTTTActgc                                                   <  1:760008/225‑1 (MQ=255)                                                                                                                                                                                                                         ttCCCGCCCCGCGTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGATCGTGGCGCATGAGCGAATGTCCTTTTATGCCTGGCTCGGGTTGGCGGAAACCGGCTTAAAACTGGGCGCAATTTTTATTCTCACGCAAATAGAAAACCACGATAAATTGATTTCTTACAGTTTACTGCTGCTGTGCGTAAGTTTATTGGtttt                          >  1:123526/1‑250 (MQ=255)                                                                                                                                                                                                                                   gcgTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGATCGTGGCGCATGAGCGAATGTCCTTTTATGCCTGGCTCGGGTTGGCGGAAACCGGCTTAAAACTGGGCGCAATTTTTATTCTCACGCAAATAGAAAACCACGATAAATTGATTTCTTACAGTTTACTGCTGCTGTGCGTAAGTTTATTGGTTTTCACCCTGTAtt               >  1:140851/1‑251 (MQ=255)                                                                                                                                                                                                                                   gcgTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGATCGTGGCGCATGAGCGAATGTCCTTTTATGCCTGGCTCGGGTTGGCGGAAACCGGCTTAAAACTGGGCGCAATTTTTATTCTCACGCAAATAGAAAACCACGATAAATTGATTTCTTACAGTTTACTGCTGCTGTGCGTAAGTTTATTGGTTTTCACCCTGTAtt               >  1:226874/1‑251 (MQ=255)                                                                                                                                                                                                                                   gcgTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTA‑CCTTTAACGCTATGATCGTGGCGCATGAGCGAATGTCCTTTTATGCCTGGCTCGGGTTGGCGGAAACCGGCTTAAAACTGGGCGCAATTTTTATTCTCACGCAAATAGAAAACCACGATAAATTGATTTCTTACAGTTTACTGCTGCTGTGCGt                                         >  1:212630/1‑224 (MQ=255)                                                                                                                                                                                                                                    cgTATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGATCGTGGCGCATGAGCGAATGTCCTTTTATGCCTGGCTCGGGTTGGCGGAAACCGGCTTAAAACTGGGCGCAATTTTTATTCTCACGCAAATAGAAAACCACGATAAATTGATTTCTTACAg                                                           >  1:445469/1‑206 (MQ=255)                                                                                                                                                                                                                                      tATGAACGCCGCCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGATCGTGGCGCATGAGCGAATGTCCTTTTATGCCTGGCTCGGGTTGGCGGAAACCGGCTTAAAACTGGGCGCAATTTTTATTCTCACGCAAATAGAAAACCACGATAAATTGATTTCTTACAGTTTACTGCTGCTGTGCGTAAGTTTATTGGTTTTCACCCTGTAtttt             >  1:841210/1‑250 (MQ=255)                                                                                                                                                                                                                                            cgccgcCAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGATCGTGGCGCATGAGCGAATGTCCTTTTATGCCTGGCTCGGGTTGGCGGAAACCGGCTTAAAACTGGGCGCAATTTTTATTCTCACGCAAATAGAAAACCACGATAAATTGATTTCTTACAGTTTACTGCTGCTGTGCGTAAGTTTATTGGTTTTCACCCTGTATTTTCGTTTCa      >  1:705519/1‑251 (MQ=255)                                                                                                                                                                                                                                              ccgccAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGATCGTGGCGCATGAGCGAATGTCCTTTTATGCCTGGCTCGGGTTGGCGGAAACCGGCTTAAAACTGGGCGCAATTTTTATTCTCACGCAAATAGAAAACCACGATAAATTGATTTCTTACAGTTTACTGCTGCTGTGCGTAAGTTTATTGGTTTTCACCCTGTATTTTCGTTTCAcc    <  1:146027/251‑1 (MQ=255)                                                                                                                                                                                                                                              ccgccAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGATCGTGGCGCATGAGCGAATGTCCTTTTATGCCTGGCTCGGGTTGGCGGAAACCGGCTTAAAACTGGGCGCAATTTTTATTCTCACGCAAATAGAAAACCACGATAAATTGATTTCTTACAGTTTACTGCTGCTGTGCGTAAGTTTATTGGTTTTCACCCTGTATTTTCGTTTCAc     >  1:506138/1‑250 (MQ=255)                                                                                                                                                                                                                                                 ccAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGATCGTGGCGCATGAGCGAATGTCCTTTTATGCCTGGCTCGGGTTGGCGGAAACCGGCTTAAAACTGGGCGCAATTTTTATTCTCACGCAAATAGAAAACCACGATAAATTGATTTCTTACAGTTTACTGCTGCTGTGCGTAAGTTTATTGGTTTTCACCCTGTATTTTCGTTTCACCCg  >  1:822977/1‑250 (MQ=255)                                                                                                                                                                                                                                                 ccAACATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGATCGTGGCGCATGAGCGAATGTCCTTTTATGCCTGGCTCGGGTTGGCGGAAACCGGCTTAAAACTGGGCGCAATTTTTATTCTCACGCAAATAGAAAACCACGATAAATTGATTTCTTACAGTTTACTGCTGCTGTGCGTAAGTTTATTGGTTTTCACCCTGTATTTTCGTTTCACCCg  >  1:841051/1‑250 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      CCATTGTGATGGGCGTGGTGATTTTGTTTTCGTTTTTCAACGGCACTATGACGGCAACCACCCAGCGTTTTATTAACGTGGAAAAAGCCTCAAACGACTCAAGCAGAGTAAATCAGGTTTTTAACATCAGCATCTTGAATCACCTGTTTATTATGCTGGTCGTGGTGATTCTGGCGGAAACGGCAGGATTATGGTTTTTAAATCATAAATTGAACATTCCCGCCCCGCGTATGAACGCCGCCAAAATTGTGTACCAGGTAGCATTGCTCATTGCGTTGGTGGAAATCATTAAAGTACCCTTTAACGCTATGATCGTGGCGCATGAGCGAATGTCCTTTTATGCCTGGCTCGGGTTGGCGGAAACCGGCTTAAAACTGGGCGCAATTTTTATTCTCACGCAAATAGAAAACCACGATAAATTGATTTCTTACAGTTTACTGCTGCTGTGCGTAAGTTTATTGGTTTTCACCCTGTATTTTCGTTTCACCCG  >  1/1696740‑1697229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑