Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,689,698	G→C	intergenic (+47340/‑92027)	unknown → / → unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,689,698	0	G	C	100.0%	131.4 / NA	47	intergenic (+47340/‑92027)	unknown/unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (25/22);  total (25/22)

CAATTGTTGGGTTAAACAGTAGCCCACTTGCCCGTTTGCGCCTGTAATCAGAAATTTTGCCATTTATTTCTCCTCGTTGATTAAACGTAGCAAGTATTGACCGTATTCATTTTTTACCATTGGTCTCGCTAATGTTTCTACTTGCTCAGAAGTTAGCCAACCATTACGCCAGGCAATTTCTTCCAAACATGCAACTTGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCGCTTCGTGTAGGCTTTCGTGCGTTCCTGTGTCTAACCACGCAAAACCACGACCGAGTAATTGCACATTTAATGAACCATCTTTTAGATACATTTCATTTAATGTGGTGATTTCAAGCTCACCGCGCGCAGATGGTTTCACCTGTTTCGCGAAATCGACCACACGATTATCGTAGAAATATAAACCTGTTACAGCATAGTTAGATTTAGGTTGAACAGGTTTTTCTTCAATAGA  >  1/1689460‑1689930                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                         cAATTGTTGGGTTAAACAGTAGCCCACTTGCCCGTTTGCGCCTGTAATCAGAAATTTTGCCATTTATTTCTCCTCGTTGATTAAACGTAGCAAGTATTGACCGTATTCATTTTTTACCATTGGTCTCGCTAATGTTTCTACTTGCTCAGAAGTTAGCCAACCATTACGCCAGGCAATTTCTTCCAAACATGCAACTTGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGc                                                                                                                                                                                                                              <  1:314993/251‑1 (MQ=255)         gggTTAAACAGTAGCCCACTTGCCCGTTTGCGCCTGTAATCAGAAATTTTGCCATTTATTTCTCCTCGTTGATTAAACGTAGCAAGTATTGACCGTATTCATTTTTTACCATTGGTCTCGCTAATGTTTCTACTTGCTCAGAAGTTAGCCAACCATTACGCCAGGCAATTTCTTCCAAACATGCAACTTGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTg                                                                                                                                                                                                                       >  1:674651/1‑250 (MQ=255)         gggTTAAACAGTAGCCCACTTGCCCGTTTGCGCCTGTAATCAGAAATTTTGCCATTTATTTCTCCTCGTTGATTAAACGTAGCAAGTATTGACCGTATTCATTTTTTACCATTGGTCTCGCTAATGTTTCTACTTGCTCAGAAGTTAGCCAACCATTACGCCAGGCAATTTCTTCCAAACATGCAACTTGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTg                                                                                                                                                                                                                       >  1:159541/1‑250 (MQ=255)         gggTTAAACAGTAGCCCACTTGCCCGTTTGCGCCTGTAATCAGAAATTTTGCCATTTATTTCTCCTCGTTGATTAAACGTAGCAAGTATTGACCGTATTCATTTTTTACCATTGGTCTCGCTAATGTTTCTACTTGCTCAGAAGTTAGCCAACCATTACGCCAGGCAATTTCTTCCAAACATGCAACTTGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTg                                                                                                                                                                                                                       >  1:377791/1‑250 (MQ=255)          ggTTAAACAGTAGCCCACTTGCCCGTTTGCGCCTGTAATCAGAAATTTTGCCATTTATTTCTCCTCGTTGATTAAACGTAGCAAGTATTGACCGTATTCATTTTTTACCATTGGTCTCGCTAATGTTTCTACTTGCTCAGAAGTTAGCCAACCATTACGCCAGGCAATTTCTTCCAAACATGCAACTTGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTGc                                                                                                                                                                                                                      <  1:509187/250‑1 (MQ=255)          ggTTAAACAGTAGCCCACTTGCCCGTTTGCGCCTGTAATCAGAAATTTTGCCATTTATTTCTCCTCGTTGATTAAACGTAGCAAGTATTGACCGTATTCATTTTTTACCATTGGTCTCGCTAATGTTTCTACTTGCTCAGAAGTTAGCCAACCATTACGCCAGGCAATTTCTTCCAAACATGCAACTTGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTGCg                                                                                                                                                                                                                     >  1:451804/1‑251 (MQ=255)                   gTAGCCCACTTGCCCGTTTGCGCCTGTAATCAGAAATTTTGCCATTTATTTCTCCTCGTTGATTAAACGTAGCAAGTATTGACCGTATTCATTTTTTACCATTGGTCTCGCTAATGTTTCTACTTGCTCAGAAGTTAGCCAACCATTACGCCAGGCAATTTCTTCCAAACATGCAACTTGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCCCTTCgtgt                                                                                                                                                                                                                                  >  1:805166/1‑229 (MQ=255)                        ccACTTGCCCGTTTGCGCCTGTAATCAGAAATTTTGCCATTTATTTCTCCTCGTTGATTAAACGTAGCAAGTATTGACCGTATTCATTTTTTACCATTGGTCTCGCTAATGTTTCTACTTGCTCAGAAGTTAGCCAACCATTACGCCAGGCAATTTCTTCCAAACATGCAACTTGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTGCGTTCCTGTGTCTAAc                                                                                                                                                                                                       <  1:693174/251‑1 (MQ=255)                         cACTTGCCCGTTTGCGCCTGTAATCAGAAATTTTGCCATTTATTTCTCCTCGTTGATTAAACGTAGCAAGTATTGACCGTATTCATTTTTTACCATTGGTCTCGCTAATGTTTCTACTTGCTCAGAAGTTAGCCAACCATTACGCCAGGCAATTTCTTCCAAACATGCAACTTGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTGCGTTCCTGTGTCTAAcc                                                                                                                                                                                                      <  1:562096/251‑1 (MQ=255)                                         ccTGTAATCAGAAATTTTGCCATTTATTTCTCCTCGTTGATTAAACGTAGCAAGTATTGACCGTATTCATTTTTTACCATTGGTCTCGCTAATGTTTCTACTTGCTCAGAAGTTAGCCAACCATTACGCCAGGCAATTTCTTCCAAACATGCAACTTGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTGCGTTCCTGTGTCTAACCACGCAAAACCACGAc                                                                                                                                                                                       <  1:878751/250‑1 (MQ=255)                                                             cATTTATTTCTCCTCGTTGATTAAACGTAGCAAGTATTGACCGTATTCATTTTTTACCATTGGTCTCGCTGATGTTTCTACTTGCTCAGAAGTTAGCCAACCATTACGCCAGGCAATTTCTTCCAAACATGCAACTTGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTGCGTTCCTGTGTCTAACCACGCAAAACCACGACCGAGTAATTGCACATTTAATg                                                                                                                                                                  <  1:25307/251‑1 (MQ=255)                                                             cATTTATTTCTCCTCGTTGATTAAACGTAGCAAGTATTGACCGTATTCATTTTTTACCATTGGTCTCGCTAATGTTTCTACTTGCTCAGAAGTTAGCCAACCATTACGCCAGGCAATTTCTTCCAAACATGCAACTTGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTGCGTTCCTGTGTCTAACCACGCAAAACCACGACCGAGTAATTGCACATTTAATg                                                                                                                                                                  <  1:866352/251‑1 (MQ=255)                                                                ttatttCTCCTCGTTGATTAAACGTAGCAAGTATTGACCGTATTCATTTTTTACCATTGGTCTCGCTAATGTTTCTACTTGCTCAGAAGTTAGCCAACCATTACGCCAGGCAATTTCTTCCAAACATGCAACTTGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTGCGTTCCTGTGTCTAACCACGCAAAACCACGACCGAGTAATTGCACATTTAATGAAc                                                                                                                                                               <  1:743540/251‑1 (MQ=255)                                                                             ttGATTAAACGTAGCAAGTATTGACCGTATTCATTTTTTACCATTGGTCTCGCTAATGTTTCTACTTGCTCAGAAGTTAGTCAACCATTACGCCAGGCAATTTCTTCCAAACATGCAACTTGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTGCGTTCCTGTGTCTAACCACGCAAAACCACGACCGAGTAATTGCACATTTAATGAACCATCTTTTAGAt                                                                                                                                                   <  1:454279/250‑1 (MQ=255)                                                                             ttGATTAAACGTAGCAAGTATTGACCGTATTCATTTTTTACCATTGGTCTCGCTAATGTTTCTACTTGCTCAGAAGTTAGCCAACCATTACGCCAGGCAATTTCTTCCAAACATGCAACTTGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTGCGTTCCTGTGTCTAACCACGCAAAACCACGACCGAGTAATTGCACATTTAATGAACCATCTTTTAGAt                                                                                                                                                   <  1:116855/250‑1 (MQ=255)                                                                             ttGATTAAACGTAGCAAGTATTGACCGTATTCATTTTTTACCATTGGTCTCGCTAATGTTTCTACTTGCTCAGAAGTTAGCCAACCATTACGCCAGGCAATTTCTTCCAAACATGCAACTTGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTGCGTTCCTGTGTCTAACCACGCAAAACCACGACCGAGTAATTGCACATTTAATGAACCATCTTTTAGAt                                                                                                                                                   <  1:577402/250‑1 (MQ=255)                                                                                    aaCGTAGCAAGTATTGACCGTATTCATTTTTTACCATTGGTCTCGCTAATGTTTCTACTTGCTCAGAAGTTAGCCAACCATTACGCCAGGCAATTTCTTCCAAACATGCAACTTGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTGCGTTCCTGTGTCTAACCACGCAAAACCACGACCGAGTAATTGCACATTTAATGAACCATCTTTTAGATAcatttca                                                                                                                                           >  1:438177/1‑251 (MQ=255)                                                                                        tAGCAAGTATTGACCGTATTCATTTTTTACCATTGGTCTCGCTAATGTTTCTACTTGCTCAGAAGTTAGCCAACCATTACGCCAGGCAATTTCTTCCAAACATGCAACTTGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTGCGTTCCTGTGTCTAACCACGCAAAACCACGACCGAGTAATTGCACATTTAATGAACCATCTTTTAGATAc                                                                                                                                                 >  1:627013/1‑241 (MQ=255)                                                                                        tAGCAAGTATTGACCGTATTCATTTTTTACCATTGGTCTCGCTAATGTTTCTACTTGCTCAGAAGTTAGCCAACCATTACGCCAGGCAATTTCTTCCAAACATGCAACTTGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTGCGTTCCTGTGTCTAACCACGCAAAACCACGACCGAGTAATTGCACATTTAATGAACCATCTTTTAGATACATTTCATTTa                                                                                                                                       >  1:70140/1‑251 (MQ=255)                                                                                           cAAGTATTGACCGTATTCATTTTTTACCATTGGTCTCGCTAATGTTTCTACTTGCTCAGAAGTTAGCCAACCATTACGCCAGGCAATTTCTTCCAAACATGCAACTTGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTGCGTTCCTGTGTCTAACCACGCAAAACCACGACCGAGTAATTGCACATTTAATGAACCATCTTTTAGATACATTTCATTTAAtg                                                                                                                                    <  1:351358/251‑1 (MQ=255)                                                                                           cAAGTATTGACCGTATTCATTTTTTACCATTGGTCTCGCTAATGTTTCTACTTGCTCAGAAGTTAGCCAACCATTACGCCAGGCAATTTCTTCCAAACATGCAACTTGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTGCGTTCCTGTGTCTAACCACGCAAAACCACGACCGAGTAATTGCACATTTAATGAACCATCTTTTAGATACATTTCATTTAAtg                                                                                                                                    <  1:169165/251‑1 (MQ=255)                                                                                                                     ccATTGGTCTCGCTAATGTTTCTACTTGCTCAGAAGTTAGCCAACCATTACGCCAGGCAATTTCTTCCAAACATGCAACTTGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTGCGTTCCTGTGTCTAACCACGCAAAACCACGACCGAGTAATTGCACATTTAATGAACCATCTTTTAGATACATTTCATTTAATGTGGTGATTTCAAGCTCAc                                                                                                                  >  1:268773/1‑243 (MQ=255)                                                                                                                          ggTCTCGCTAATGTTTCTACTTGCTCAGAAGTTAGCCAACCATTACGCCAGGCAATTTCTTCCAAACATGCAACTTGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTGCGTTCCTGTGTCTAACCACGCAAAACCACGACCGAGTAATTGCACATTTAATGAACCATCTTTTAGATACATTTCATTTAATGTGGTGATTTCAAGCTCACCGCGCGCAGATgg                                                                                                     >  1:62114/1‑251 (MQ=255)                                                                                                                               cGCTAATGTTTCTACTTGCTCAGAAGTTAGCCAACCATTACGCCAGGCAATTTCTTCCAAACATGCAACTTGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTGCGTTCCTGTGTCTAACCACGCAAAACCACGACCGAGTAATTGCACATTTAATGAACCATCtttt                                                                                                                                                       <  1:604499/196‑1 (MQ=255)                                                                                                                                     tGTTTCTACTTGCTCAGAAGTTAGCCAACCATTACGCCAGGCAATTTCTTCCAAACATGCAACTTGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTGCGTTCCTGTGTCTAACCACGCAAAACCACGACCGAGTAATTGCACATTTAATGAACCATCTTTTAGATACATTTCATTTAATGTGGTGATTTCAAGCTCACCGCGCGCAGATGGtt                                                                                                   >  1:21787/1‑242 (MQ=255)                                                                                                                                                gCTCAGAAGTTAGCCAACCATTACGCCAGGCAATTTCTTCCAAACATGCAACTTGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTGCGTTCCTGTGTCTAACCACGCAAAACCACGACCGAGTAATTg                                                                                                                                                                             <  1:474643/157‑1 (MQ=255)                                                                                                                                                gCTCAGAAGTTAGCCAACCATTACGCCAGGCAATTTCTTCCAAACATGCAACTTGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTGCGTTCCTGTGTCTAACCACGCAAAACCACGACCGAGTAATTGCACATTTAATGAACCATCTTTTAGATACATTTCATTTAATGTGGTGATTTCAAGCTCACCGCGCGCAGATGGTTTCACCTGTTTCGCGAAATCg                                                                               >  1:468197/1‑251 (MQ=255)                                                                                                                                                gCTCAGAAGTTAGCCAACCATTACGCCAGGCAATTTCTTCCAAACATGCAACTTGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTGCGTTCCTGTGTCTAACCACGCAAAACCACGACCGAGTAATTGCACATTTAATGAACCATCTTTTAGATACATTTCATTTAATGTGGTGATTTCAAGCTCACCGCGCGCAGATGGTTTCACCTGTTTCGCGAAATCg                                                                               >  1:60153/1‑251 (MQ=255)                                                                                                                                                        gTTAGCCAACCATTACGCCAGGCAATTTCTTCCAAACATGCAACTTGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTGCGt                                                                                                                                                                                                                    >  1:239849/1‑110 (MQ=255)                                                                                                                                                        gTTAGCCAACCATTACGCCAGGCAATTTCTTCCAAACATGCAACTTGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTGCGTTCCTGTGTCTAACCACGCAAAACCACGACCGAGTAATTGCACATTTAATGAACCATCTTTTAGATACATTTCATTTAATGTGGTGATTTCAAGCTCACCGCGCGCAGATGGTTTCACCTGTTTCGCGAAATCGACCACACg                                                                       >  1:857564/1‑251 (MQ=255)                                                                                                                                                                 ccATTACGCCAGGCAATTTCTTCCAAACATGCAACTTGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTGCGTTCCTGTGTCTAACCACGCAAAACCACGACCGAGTAATTGCACATTTAATGAACCATCTTTTAGATACATTTCATTTAATGTGGTGa                                                                                                                              >  1:437834/1‑187 (MQ=255)                                                                                                                                                                 ccATTACGCCAGGCAATTTCTTCCAAACATGCAACTTGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTGCGTTCCTGTGTCTAACCACGCAAAACCACGACCGAGTAATTGCACATTTAATGAACCATCTTTTAGATACATTTCATTTAATGTGGTGATTTCAAGCTCACCGCGCGCAGATg                                                                                                      <  1:376582/211‑1 (MQ=255)                                                                                                                                                                 ccATTACGCCAGGCAATTTCTTCCAAACATGCAACTTGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTGCGTTCCTGTGTCTAACCACGCAAAACCACGACCGAGTAATTGCACATTTAATGAACCATCTTTTAGATACATTTCATTTAATGTGGTGATTTCAAGCTCACCGCGCGCAGATGGTTTCAcc                                                                                              >  1:73612/1‑219 (MQ=255)                                                                                                                                                                 ccATTACGCCAGGCAATTTCTTCCAAACATGCAACTTGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTGCGTTCCTGTATCTAACCACGCAAAACCACGACCGAGTAATTGCACATTTAATGAACCATCTTTTAGATACATTTCATTTAATGTGGTGATTTCAAGCTCACCGCGCGCAGATGGTTTCACCTGTTTCGCGAAATCGACCACACGATTAt                                                                  >  1:63979/1‑247 (MQ=255)                                                                                                                                                                 ccATTACGCCAGGCAATTTCTTCCAAACATGCAACTTGCAAACCTTGTACGCTTTCAACAGCGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTGCGTTCCTGTGTCTAACCACGCAAAACCACGACCGAGTAATTGCACATTTAATGAACCATCTTTTAGATACATTTCATTTAATGTGGTGATTTCAAGCTCACCGCGCGCAGATGGTTTCACCTGTTTCGCGAAATCGACCACACGATTATCGTa                                                              >  1:185375/1‑251 (MQ=255)                                                                                                                                                                              cAATTTCTTCCAAACATGCAACTTGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTGCGTTCCTGTGTCTAACCACGCAAAACCACGACCGAGTAATTGCACATTTAATGAACCATCTTTTAGATACATTTCATTTAATGTGGTGATTTCAAGCTCACCGCGCGCAGATGGTTTCACCTGTTTCGCGAAATCGACCACACGATTATCGTAGAAATATAAAcc                                                  <  1:87574/250‑1 (MQ=255)                                                                                                                                                                              cAATTTCTTCCAAACATGCAACTTGCAAACCTTGTACGCTTTCAACAGTGCGAAAGAAAGATGCCCCTTCGTGTAGGCTTTCGTGCGTTCCTGTGTCTAACCACGCAAAACCACGACCGAGTAATTGCACATTTAATGAACCATCTTTTAGATACATTTCATTTAATGTGGTGATTTCAAGCTCACCGCGCGCAGATGGTTTCACCTGTTTCGCGAAATCGACCACACGATTATCGTAGAAATATAAAcc                                                  <  1:134274/250‑1 (MQ=255)                                                                                                                                                                                   tcttcCAAACATGCAACTTGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTGCGTTCCTGTGTCTAACCACGCAAAACCACGACCGAGTAATTGCACATTTAATGAACCATCTTTTAGATACATTTCATTTAATGTGGTGATTTCAAGCTCACCGCGCGCAGATGGTTTCACCTGTTTCGCGAAATCGACCACACGATTATCGTAGAAATATAAACCTGTTAc                                            <  1:682858/251‑1 (MQ=255)                                                                                                                                                                                    cttcCAAACATGCAACTTGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTGCGTTCCTGTGTCTAACCACGCAAAAcc                                                                                                                                                                                            >  1:167195/1‑106 (MQ=255)                                                                                                                                                                                        cAAACATGCAACTTGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTGCGTTCCTGTGTCTAACCACGCAAAACCACGACCGAGTAATTGCACATTTAATGAACCATCTTTTAGATACATTTCATTTAATGTGGTGATTTCAAGCTCACCGCGCGCAGATGGTTTCACCTGTTTCGCGAAATCGACCACACGATTATCGTAGAAATATAAACCTGTTACAGCAt                                       <  1:137790/251‑1 (MQ=255)                                                                                                                                                                                           aCATGCAACTTGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTGCGTTCCTGTGTCTAACCACGCAAAACCACGACCGAGTAATTGCACATTTAATGAACCATCTTTTAGATACATTTCATTTAATGTGGTGATTTCAAGCTCACCGCGCGCAGATGGTTTCACCTGTTTCGCGAAATCGACCACACGATTATCGTAGAAATATAAACCTGTTACAGCa                                        <  1:519711/247‑1 (MQ=255)                                                                                                                                                                                                  aCTTGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTGCGTTCCTGTGTCTAACCACGCAAAACCACGACCGAGTAATTGCACATTTAATGAACCATCTTTTAGATACATTTCATTTAATGTGGTGATTTCAAGCTCACCGCGCGCAGATGGTTTCACCTGTTTCGCGAAATCGACCACACGATTATCGTAGAAATATAAAcc                                                  >  1:64321/1‑230 (MQ=255)                                                                                                                                                                                                     tGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTGCGTTCCTGTGTCTAACCACGCAAAACCACGACCGAGTAATTGCACATTTAATGAACCATCTTTTAGATACATTTCATTTAATGTGGTGATTTCAAGCTCACCGCGCGCAGATGGTTTCACCTGTTTCGCGAAATCGACCACACGATTATCGTAGAAATATAAACCTGTTACAGCATAGTTAGATTTAgg                          >  1:238713/1‑251 (MQ=255)                                                                                                                                                                                                           ccTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTGCGTTCCTGTGTCTAACCACGCAAAACCACGACCGAGTAATTGCACATTTAATGAACCATCTTTTAGATACATTTCATTTAATGTGGTGATTTCAAGCTCACCGCGCGCAGATGGTTTCACCTGTTTCGCGAAATCGACCACACGATTATCGTAGAAATATAAACCTGTTACAGCAt                                       >  1:636793/1‑232 (MQ=255)                                                                                                                                                                                                                   gCTTTCAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTGCGTTCCTGTGTCTAACCACGCAAAACCACGACCGAGTAATTGCACATTTAATGAAc                                                                                                                                                               <  1:337265/104‑1 (MQ=255)                                                                                                                                                                                                                        cAACAGTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTGCGTTCCTGTGTCTAACCACGCAAAACCACGACCGAGTAATTGCACATTTAATGAACCATCTTTTAGATACATTTCATTTAATGTGGTGATTTCAAGCTCACCGCGCGCAGATGGTTTCACCTGTTTCGCGAAATCGACCACACGATTATCGTAGAAATATAAACCTGTTACAGCATAGTTAGATTTAGGTTGAGCAGGTTTTTCTTCa       >  1:636731/1‑251 (MQ=255)                                                                                                                                                                                                                             gTGCGAACGAAAGATGCCCCTTCGTGTAGGCTTTCGTGCGTTCCTGTGTCTAACCACGCAAAACCACGACCGAGTAATTGCACATTTAATGAACCATCTTTTAGATACATTTCATTTAATGTGGTGATTTCAAGCTCACCGCGCGCAGATGGTTTCACCTGTTTCGCGAAATCGACCACACGATTATCGTAGAAATATAAACCTGTTACAGCATAGTTAGATTTAGGTTGAGCAGGTTTTTCTTCAATAGa  >  1:489656/1‑251 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                         CAATTGTTGGGTTAAACAGTAGCCCACTTGCCCGTTTGCGCCTGTAATCAGAAATTTTGCCATTTATTTCTCCTCGTTGATTAAACGTAGCAAGTATTGACCGTATTCATTTTTTACCATTGGTCTCGCTAATGTTTCTACTTGCTCAGAAGTTAGCCAACCATTACGCCAGGCAATTTCTTCCAAACATGCAACTTGCAAACCTTGTACGCTTTCAACAGTGCGAACGAAAGATGCCGCTTCGTGTAGGCTTTCGTGCGTTCCTGTGTCTAACCACGCAAAACCACGACCGAGTAATTGCACATTTAATGAACCATCTTTTAGATACATTTCATTTAATGTGGTGATTTCAAGCTCACCGCGCGCAGATGGTTTCACCTGTTTCGCGAAATCGACCACACGATTATCGTAGAAATATAAACCTGTTACAGCATAGTTAGATTTAGGTTGAACAGGTTTTTCTTCAATAGA  >  1/1689460‑1689930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑