Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,670,779	A→G	intergenic (+28421/‑110946)	unknown → / → unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,670,779	0	A	G	100.0%	222.3 / NA	74	intergenic (+28421/‑110946)	unknown/unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (43/31);  total (43/31)

TTATTATGATGCGCCAAAAGGTGCGATTTATTTGAAGGATATTCGCGTACTACGCTGGTCCGGCTCGCCTGAACAATACATGGGGCAAATTCAAAGTCTGGTGCCGTTACTCACCAACAATATCGCCACCTATCTGAGCAACACGCCGGTTTATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGACACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGC  >  1/1670540‑1671021                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                   ttattaTGATGCGCCAAAAGGTGCGATTTATTTGAAGGATATTCGCGTACTACGCTGGTCCGGCTCGCCTGAACAATACATGGGGCAAATTCAAAGTCTGGTGCCGTTACTCACCAACAATATCGCCACCTATCTGAGCAACACGCCGGTTTATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATa                                                                                                                                                                                                                                         >  1:2357/1‑251 (MQ=255) ttattaTGATGCGCCAAAAGGTGCGATTTATTTGAAGGATATTCGCGTACTACGCTGGTCCGGCTCGCCTGAACAATACATGGGGCAAATTCAAAGTCTGGTGCCGTTACTCACCAACAATATCGCCACCTATCTGAGCAACACGCCGGTTTATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATa                                                                                                                                                                                                                                         >  1:657570/1‑251 (MQ=255) ttattaTGATGCGCCAAAAGGTGCGATTTATTTGAAGGATATTCGCGTACTACGCTGGTCCGGCTCGCCTGAACAATACATGGGGCAAATTCAAAGTCTGGTGCCGTTACTCACCAACAATATCGCCACCTATCTGAGCAACACGCCGGTTTATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATa                                                                                                                                                                                                                                         >  1:833664/1‑251 (MQ=255)                   aGGTGCGATTTATTTGAAGGATATTCGCGTACTACGCTGGTCCGGCTCGCCTGAACAATACATGGGGCAAATTCAAAGTCTGGTGCCGTTACTCACCAACAATATCGCCACCTATCTGAGCAACACGCCGGTTTATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATaa                                                                                                                                                                                                                                        >  1:12272/1‑234 (MQ=255)                          atttatttGAAGGATATTCGCGTACTACGCTGGTCCGGCTCGCCTGAACAATACATGGGGCAAATTCAAAGTCTGGTGCCGTTACTCACCAACAATAGCGCCTCCTATCTGAGCAACACGCCGGTTTATACCCTGGAGGAAAAAAATCCGCGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATa                                                                                                                                                                                                                                         <  1:631753/226‑1 (MQ=255)                                ttGAAGGATATTCGCGTACTACGCTGGTCCGGCTCGCCTGAACAATACATGGGGCAAATTCAAAGTCTGGTGCCGTTACTCACCAACAATATCGCCACCTATCTGAGCAACACGCCGGTTTATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAAt                                                                                                                                                                                                          <  1:322386/251‑1 (MQ=255)                                     ggATATTCGCGTACTACGCTGGTCCGGCTCGCCTGAACAATACATGGGGCAAATTCAAAGTCTGGTGCCGTTACTCACCAACAATATCGCCACCTATCTGAGCAACACGCCGGTTTATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAgg                                                                                                                                                                                                     >  1:514111/1‑251 (MQ=255)                                     ggATATTCGCGTACTACGCTGGTCCGGCTCGCCTGAACAATACATGGGGCAAATTCAAAGTCTGGTGCCGTTACTCACCAACAATATCGCCACCTATCTGAGCAACACGCCGGTTTATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAgg                                                                                                                                                                                                     >  1:834587/1‑251 (MQ=255)                                       atatTCGCGTACTACGCTGGTCCGGCTCGCCTGAACAATACATGGGGCAAATTCAAAGTCTGGTGCCGTTACTCACCAACAATATCGCCACCTATCTGAGCAACACGCCGGTTTATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAAcc                                                                                                                                                                                                                            >  1:436139/1‑226 (MQ=255)                                         atTCGCGTACTACGCTGGTCCGGCTCGCCTGAACAATACATGGGGCAAATTCAAAGTCTGGTGCCGTTACTCACCAACAATATCGCCACCTATCTGAGCAACACGCCGGTTTATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATa                                                                                                                                                                                                                                         >  1:734669/1‑211 (MQ=255)                                              cgTACTACGCTGGTCCGGCTCGCCTGAACAATACATGGGGCAAATTCAAAGTCTGGTGCCGTTACTCACCAACAATATCGCCACCTATCTGAGCAACACGCCGGTTTATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACaa                                                                                                                                                                                             <  1:357925/250‑1 (MQ=255)                                              cgTACTACGCTGGTCCGGCTCGCCTGAACAATACATGGGGCAAATTCAAAGTCTGGTGCCGTTACTCACCAACAATATCGCCACCTATCTGAGCAACACGCCGGTTTATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAg                                                                                                                                                                                            >  1:746665/1‑251 (MQ=255)                                               gTACTACGCTGGTCCGGCTCGCCTGAACAATACATGGGGCAAATTCAAAGTCTGGTGCCGTTACTCCCCAACAATATCGCCACCTATCTGAGCAACACGCCGGTTTATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAAcc                                                                                                                                                                                                                            <  1:321579/218‑1 (MQ=255)                                               gTACTACGCTGGTCCGGCTCGCCTGAACAATACATGGGGCAAATTCAAAGTCTGGTGCCGTTACTCACCAACAATATCGCCACCTATCTGAGCAACACGCCGGTTTATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAgg                                                                                                                                                                                           >  1:373835/1‑251 (MQ=255)                                                tactacGCTGGTCCGGCTCGCCTGAACAATACATGGGGCAAATTCAAAGTCTGGTGCCGTTACTCACCAACAATATCGCCACCTATCTGAGCAACACGCCGGTTTATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGa                                                                                                                                                                                          >  1:728008/1‑251 (MQ=255)                                                  ctacGCTGGTCCGGCTCGCCTGAACAATACATGGGGCAAATTCAAAGTCTGGTGCCGTTACTCACCAACAATATCGCCACCTATCTGAGCAACACGCCGGTTTATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAggttt                                                                                                                                                                                        >  1:862262/1‑248 (MQ=255)                                                         ggTCCGGCTCGCCTGAACAATACATGGGGCAAATTCAAAGTCTGGTGCCGTTACTCACCAACAATATCGCCACCTATCTGAGCAACACGCCGGTTTATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCtt                                                                                                                                                                                 >  1:38817/1‑251 (MQ=255)                                                               gCTCGCCTGAACAATACATGGGGCAAATTCAAAGTCTGGTGCCGTTACTCACCAACAATATCGCCACCTATCTGAGCAACACGCCGGTTTATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCttttttt                                                                                                                                                                            <  1:173408/250‑2 (MQ=255)                                                                  cGCCTGAACAATACATGGGGCAAATTCAAAGTCTGGTGCCGTTACTCACCAACAATATCGCCACCTATCTGAGCAACACGCCGGTTTATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTg                                                                                                                                                                         >  1:617928/1‑250 (MQ=255)                                                                       gAACAATACATGGGGCAAATTCAAAGTCTGGTGCCGTTACTCACCAACAATATCGCCACCTATCTGAGCAACACGCCGGTTTATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTaa                                                                                                                                                                   >  1:826730/1‑251 (MQ=255)                                                                            aTACATGGGGCAAATTCAAAGTCTGGTGCCGTTACTCACCAACAATATCGCCACCTATCTGAGCAACACGCCGGTTTATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACAtt                                                                                                                                                              >  1:443886/1‑251 (MQ=255)                                                                            aTACATGGGGCAAATTCAAAGTCTGGTGCCGTTACTCACCAACAATATCGCCACCTATCTGAGCAACACGCCGGTTTATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACAtt                                                                                                                                                              >  1:374494/1‑251 (MQ=255)                                                                            aTACATGGGGCAAATTCAAAGTCTGGTGCCGTTACTCACCAACAATATCGCCACCTATCTGAGCAACACGCCGGTTTATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACAtt                                                                                                                                                              >  1:417483/1‑251 (MQ=255)                                                                                   gggCAAATTCAAAGTCTGGTGCCGTTACTCACCAACAATATCGCCACCTATCTGAGCAACACGCCGGTTTATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGa                                                                                                                                                       >  1:633777/1‑251 (MQ=255)                                                                                        aaTTCAAAGTCTGGTGCCGTTACTCACCAACAATATCGCCACCTATCTGAGCAACACGCCGGTTTATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACAt                                                                                                                                                               <  1:796052/238‑1 (MQ=255)                                                                                         aTTCAAAGTCTGGTGCCGTTACTCACCAACAATATCGCCACCTATCTGAGCAACACGCCGGTTTATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCtttttt                                                                                                                                                                             <  1:3996/223‑1 (MQ=255)                                                                                         aTTCAAAGTCTGGTGCCGTTACTCACCAACAATATCGCCACCTATCTGAGCAACACGCCGGTTTATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATaaaaa                                                                                                                                                 <  1:875970/251‑1 (MQ=255)                                                                                          ttCAAAGTCTGGTGCCGTTACTCACCAACAATATCGCCACCTATCTGAGCAACACGCCGGTTTATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAaac                                                                                                                                                <  1:374081/251‑1 (MQ=255)                                                                                                gTCTGGTGCCGTTACTCACCAACAATATCGCCACCTATCTGAGCAACACGCCGGTTTATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAaac                                                                                                                                                <  1:138355/245‑1 (MQ=255)                                                                                                 tCTGGTGCCGTTACTCACCAACAATATCGCCACCTATCTGAGCAACACGCCGGTTTATACCCTAGACGAAAAAAATCCATGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAAcgc                                                                                                                                           <  1:455626/249‑1 (MQ=255)                                                                                                        ccGTTACTCACCAACAATATCGCCACCTATCTGAGCAACACGCCGGTTTATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGgcgc                                                                                                                                   >  1:407181/1‑250 (MQ=255)                                                                                                                  ccAACAATATCGCCACCTATCTGAGCAACACGCCGGTTTATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAACCAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAACGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACg                                                                                                                                                          >  1:126417/1‑217 (MQ=255)                                                                                                                     acaaTATCGCCACCTATCTGAGCAACACGCCGGTTTATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAAGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGccgccg                                                                                                                     >  1:438949/1‑251 (MQ=255)                                                                                                                      caaTATCGCCACCTATCTGAGCAACACGCCGGTTTATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCcacgcac                                                                                                                            >  1:325803/1‑243 (MQ=255)                                                                                                                      caaTATCGCCACCTATCTGAGCAACACGCCGGTTTATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGACTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAaac                                                                                                                                                >  1:429999/1‑223 (MQ=255)                                                                                                                        atatCGCCACCTATCTGAGCAACACGCCGGTTTATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTGAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAg                                                                                                                  <  1:477058/251‑1 (MQ=255)                                                                                                                        atatCGCCACCTATCTGAGCAACACGCCGGTTTATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAg                                                                                                                  <  1:209070/251‑1 (MQ=255)                                                                                                                        atatCGCCACCTATCTGAGCAACACGCCGGTTTATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAg                                                                                                                  <  1:552400/251‑1 (MQ=255)                                                                                                                          atCGCCACCTATCTGAGCAACACGCCGGTTTATACCCTAGACG‑AAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCg                                                                                                               >  1:306604/1‑251 (MQ=255)                                                                                                                              ccACCTATCTGAGCAACACGCCGGTTTATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTg                                                                                                                                                                         >  1:219978/1‑190 (MQ=255)                                                                                                                                  cTATCTGAGCAACACGCCGGTTTATACCCTAGACGAAAAAAACCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATAtt                                                                                                        <  1:513720/251‑1 (MQ=255)                                                                                                                                   tATCTGAGCAACACGCCGGTTTATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAaac                                                                                                                                                <  1:569890/210‑1 (MQ=255)                                                                                                                                                    ggTTTATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAaac                                                                                                                                                >  1:664259/1‑193 (MQ=255)                                                                                                                                                    ggTTTATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAAcgc                                                                                                                                           <  1:346520/198‑1 (MQ=255)                                                                                                                                                      tttATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATaaaaa                                                                                                                                                 >  1:814340/1‑190 (MQ=255)                                                                                                                                                                  aCGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGgcgc                                                                          <  1:234980/249‑1 (MQ=255)                                                                                                                                                                      aaaaaaTCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATAtt                                                                                                        >  1:285082/1‑215 (MQ=255)                                                                                                                                                                             ccACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATg                                                             <  1:753780/251‑1 (MQ=255)                                                                                                                                                                             ccACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATg                                                             <  1:473144/251‑1 (MQ=255)                                                                                                                                                                             ccACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATg                                                             <  1:233793/251‑1 (MQ=255)                                                                                                                                                                                cGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATc                                                                                          <  1:144951/219‑1 (MQ=255)                                                                                                                                                                                  ggATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCgcgg                                                                                                                                      >  1:396939/1‑173 (MQ=255)                                                                                                                                                                                  ggATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAg                                                                                                                  >  1:642530/1‑193 (MQ=255)                                                                                                                                                                                  ggATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGc                                                                                                    >  1:533176/1‑207 (MQ=255)                                                                                                                                                                                  ggATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCAc                                                                                  <  1:512148/225‑1 (MQ=255)                                                                                                                                                                                  ggATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATgcggcg                                                        >  1:668233/1‑251 (MQ=255)                                                                                                                                                                                      atgatgATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATg                                                             >  1:893459/1‑242 (MQ=255)                                                                                                                                                                                          tgatTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTAt                                                                                                                                                                           >  1:631415/1‑128 (MQ=255)                                                                                                                                                                                          tgatTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCcacgc                                                                                                                              >  1:355049/1‑173 (MQ=255)                                                                                                                                                                                           gatTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCtt                                               >  1:727821/1‑251 (MQ=255)                                                                                                                                                                                               aaaCAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCgg                                           >  1:233213/1‑251 (MQ=255)                                                                                                                                                                                                      ccGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCa                                    >  1:90914/1‑251 (MQ=255)                                                                                                                                                                                                       cGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCAc                                   <  1:47241/251‑1 (MQ=255)                                                                                                                                                                                                       cGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCAc                                   <  1:679210/251‑1 (MQ=255)                                                                                                                                                                                                            aaGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGtat                              <  1:515682/251‑1 (MQ=255)                                                                                                                                                                                                            aaGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGtat                              <  1:211432/251‑1 (MQ=255)                                                                                                                                                                                                                cATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTataaat                          <  1:883919/251‑1 (MQ=255)                                                                                                                                                                                                                cATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTataaat                          <  1:393209/251‑1 (MQ=255)                                                                                                                                                                                                                cATTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTataaa                           <  1:351456/250‑1 (MQ=255)                                                                                                                                                                                                                 aTTCGGGTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTataaata                         <  1:752291/251‑1 (MQ=255)                                                                                                                                                                                                                     gggTAGAACAAGGCAGATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGgcgc                                                                          >  1:364913/1‑198 (MQ=255)                                                                                                                                                                                                                                    gATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGtat                              >  1:58399/1‑227 (MQ=255)                                                                                                                                                                                                                                    gATTGGAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATccg      >  1:619144/1‑251 (MQ=255)                                                                                                                                                                                                                                         gAAGTGGGCACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATTATTGAAATTGGACAATccgccgc  >  1:832750/1‑250 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                   TTATTATGATGCGCCAAAAGGTGCGATTTATTTGAAGGATATTCGCGTACTACGCTGGTCCGGCTCGCCTGAACAATACATGGGGCAAATTCAAAGTCTGGTGCCGTTACTCACCAACAATATCGCCACCTATCTGAGCAACACGCCGGTTTATACCCTAGACGAAAAAAATCCACGGGATATGATGATTAAACAAGCCGCTAAAGGCATTCGGGTAGAACAAGGCAGATTGGAAGTGGACACCTTTGATAATTTGCTAAAACCATAATTTCAGGCAAAAATAAAGGCTGAACAAGGATTCAGCCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGC  >  1/1670540‑1671021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑