Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,652,688	G→A	intergenic (+10330/‑129037)	unknown → / → unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,652,688	0	G	A	100.0%	28.7 / NA	12	intergenic (+10330/‑129037)	unknown/unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/7);  total (5/7)

TAGTAAGAGCAAGTACGCCGGTTAATAAATTAATTTTTTTCATGATAAACTCCTTAAATAATAGTATTAAATGTAAAAATATTTTGCTTGAATAAGCACGAAGTGAAAAAGTCATAATAAACTCCCACACACATAATGTTAGACGGAGACATTTCCATTTTCTTACAAAAAATTTTTTAAAATTTGCAAATCAACCTAAAAAACAGATACTCTCATATAAAAATATATTTATAGTGAGCGCTCTAAATAGATATTTAATTACTAAAATCTATTTCATCCATTGTTTTTTCAAATTGAATTTATTGATTTAAATCATATGTTTCCTCTTATATTTAACCGATAATCCCTTCTTCGTTTTTACTTTTAAGTGTTGGAGGACAATTATGAAAAAATTTGCACTGAAAACGGCGGTTGTCGCTATTGTTGGCTATTTAGGAGTTGTAGGGTACGTTCATCAGTATGATAAAGGACAAATG  >  1/1652451‑1652926                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                               tAGTAAGAGCAAGTACGCCGGTTAATAAATTAATTTTTTTCATGATAAACTCCTTAAATAATAGTATTAAATGTAAAAATATTTTGCTTGAATAAGCACGAAGTGAAAAAGTCATAATAAACTCCCACACACATAATGTTAGACGGAGACATTTCCATTTTCTTACAAAAAATTTTTTAAAATTTGCAAATCAACCTAAAAAACAGATACTCTCATATAAAAATATATTTATAGTGAACGCTCTAAATa                                                                                                                                                                                                                                     >  1:348316/1‑249 (MQ=255)                                                                                                                            cccACACACATAATGTTAGACGGAGACATTTCCATTTTCTTACAAAAAATTTTTTAAAATTTGCAAATCAACCTAAAAAACAGATACTCTCATATAAAAATATATTTATAGTGAACGCTCTAAATa                                                                                                                                                                                                                                     >  1:592286/1‑126 (MQ=255)                                                                                                                                             aGACGGAGACATTTCCATTTTCTTACAAAAAATTTTTTAAAATTTGCAAATCAACCTAAAAAACAGATACTCTCATATAAAAATATATTTATAGTGAACGCTCTAAATAGATATTTAATTACTAAAATCTATTTCATCCATTGTTTTTTCAAATTGAATTTATTGATTTAAATCATATGTTTCCTCTTATATTTAACCGATAATCCCTTCTTCGTTTTTACTTTTAAGTGTTGGAGGACAATTATGaaaaa                                                                                       >  1:74917/1‑251 (MQ=255)                                                                                                                                                      cATTTCCATTTTCTTACAAAAAATTTTTTAAAATTTGCAAATCAACCTAAAAAACAGATACTCTCATATAAAAATATATTTATAGTGAACGCTCTAAATAGa                                                                                                                                                                                                                                   <  1:82592/102‑1 (MQ=255)                                                                                                                                                                                tttAAAATTTGCAAATCAACCTAAAAAACAGATACTCTCATATAAAAATATATTTATAGTGAACGCTCTAAATAGATATTTAATTACTAAAATCTATTTCATCCATTGTTTTTTCAAATTGAATTTATTGATTTAAATCATATGTTTCCTCTTATATTTAACCGATAATCCCTTCTTCGTTTTTACTTTTAAGTGTTGGAGGACAATTATGAAAAAATTTGCACTGAAAACGGCGGTTGTCGCTAttgttg                                                    <  1:573517/251‑1 (MQ=255)                                                                                                                                                                                            aaaTCAACCTAAAAAACAGATACTCTCATATAAAAATATATTTATAGTGAACGCTCTAAATAGATATTTAATTACTAAAATCTATTTCATCCATTGTTTTTTCAAATTGAATTTATTGATTTAAATCATATGTTTCCTCTTATATTTAACCGATAATCCCTTCTTCGTTTTTACTTTTAAGTGTTGGAGGACAATTATGAAAAAATTTGCACTGAAAACGGTGGTTGTCGCTATTGTTGGCTATTTAGGAg                                        <  1:220556/251‑1 (MQ=255)                                                                                                                                                                                                                   tctcATATAAAAATATATTTATAGTGAACGCTCTAAATAGATATTTAATTACTAAAATCTATTTCATCCATTGTTTTTTCAAATTGAATTTATTGATTTAAATCATATGTTTCCTCTTATATTTAACCGATAATCCCTTCTTCGTTTTTACTTTTAAGTGTTGGAGGACAATTATGAAAAAATTTGCACTGAAAACGGCGGTTGTCGCTATTGTTGGCTATTTAGGAGTTGTAGGGTACGTTCATCAGTAt                 <  1:234603/251‑1 (MQ=255)                                                                                                                                                                                                                        tataAAAATATATTTATAGTGAACGCTCTAAATAGATATTTAATTACTAAAATCTATTTCATCCATTGTTTTTTCAAATTGAATTTATTGATTTAAATCATATGTTTCCTCTTATATTTAACCGATAATCCCTTCTTCGTTTTTACTTTTAAGTGTTGGAGGACAATTATGAAAAAATTTGCACTGAAAACGGCGGTTGTCGCTATTGTTGGCTATTTAGGAGTTGTAGGGTACGTTCATCAGTATGATaa            <  1:602132/251‑1 (MQ=255)                                                                                                                                                                                                                             aaaTATATTTATAGTGAACGCTCTAAATAGATATTTAATTACTAAAATCTATTTCATCCATTGTTTTTTCAAATTGAATTTATTGATTTAAATCATATGTTTCCTCTTATATTTAACCGATAATCCCTTCTTCGTTTTTACTTTTAAGTGTTGGAGGACAATTATGAAAAAATTTGCACTGAAAACGGCGGTTGTCGCTATTGTTGGCTATTTAGGAGTTGTAGGGTACGTTCATCAGTATGATAAAGGAc       <  1:595476/251‑1 (MQ=255)                                                                                                                                                                                                                                  tatTTATAGTGAACGCTCTAAATAGATATTTAATTACTAAAATCTATTTCATCCATTGTTTTTTCAAATTGAATTTATTGATTTAAATCATATGTTTCCTCTTATATTTAACCGATAATCCCTTCTTCGTTTTTACTTTTAAGTGTTGGAGGACAATTATGAAAAAATTTGCACTGAAAACGGCGGTTGt                                                               <  1:611379/190‑1 (MQ=255)                                                                                                                                                                                                                                  tatTTATAGTGAACGCTCTAAATAGATATTTAATTACTAAAATCTATTTCATCCATTGTTTTTTCAAATTGAATTTATTGATTTAAATCATATGTTTCCTCTTATATTTAACCGATAATCCCTTCTTCGTTTTTACTTTTAAGTGTTGGAGGACAATTATGAAAAAATTTGCACTGAAAACGGCGGTTGTCGCTATTTTTGGCTATTTAGGAGTTGTAGGGGACGtt                          >  1:718123/1‑227 (MQ=255)                                                                                                                                                                                                                                  tatTTATAGTGAACGCTCTAAATAGATATTTAATTACTAAAATCTATTTCATCCATTGTTTTTTCAAATTGAATTTATTGATTTAAATCATATGTTTCCTCTTATATTTAACCGATAATCCCTTCTTCGTTTTTACTTTTAAGTGTTGGAGGACAATTATGAAAAAATTTGCACTGAAAACGGCGGTTGTCGCTATTGTTGGCTATTTAGGAGTTGTAGGGTACGTTCATCAGTATGATaaatgtctcttt  >  1:65875/1‑242 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                               TAGTAAGAGCAAGTACGCCGGTTAATAAATTAATTTTTTTCATGATAAACTCCTTAAATAATAGTATTAAATGTAAAAATATTTTGCTTGAATAAGCACGAAGTGAAAAAGTCATAATAAACTCCCACACACATAATGTTAGACGGAGACATTTCCATTTTCTTACAAAAAATTTTTTAAAATTTGCAAATCAACCTAAAAAACAGATACTCTCATATAAAAATATATTTATAGTGAGCGCTCTAAATAGATATTTAATTACTAAAATCTATTTCATCCATTGTTTTTTCAAATTGAATTTATTGATTTAAATCATATGTTTCCTCTTATATTTAACCGATAATCCCTTCTTCGTTTTTACTTTTAAGTGTTGGAGGACAATTATGAAAAAATTTGCACTGAAAACGGCGGTTGTCGCTATTGTTGGCTATTTAGGAGTTGTAGGGTACGTTCATCAGTATGATAAAGGACAAATG  >  1/1652451‑1652926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑