Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,645,622	T→C	intergenic (+3264/‑136103)	unknown → / → unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,645,622	0	T	C	100.0%	178.6 / NA	62	intergenic (+3264/‑136103)	unknown/unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/25);  total (37/25)

GAATGACATTTACCCACAACCGCTTCAAATTCGGACATATTCGGCGTTAATAAGGTCGCACCGCGATAGCGTTCAAAATCCGTCCCTTTCGGATCGATGAGCACCGGCACATTGGCGTTACGGGCAATTTGGATCATTTTTTGTACTTCATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCATGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTAATTGTCGGGTGGGTAGCAAGCTGTACGAAGTCGCAAGCGATTTTTTGTTGTTGTAATAATGAGGTTAACGCCGCGCCCGCTTCATCCTGACCGGTTAAGCCCAGCAATTGCACCGGCACATTTAACGAAGCGATGTTCATTGCCACGTTTGCCGCACCGCCTGCACG  >  1/1645381‑1645859                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                              gAATGACATTTACCCACAACCGCTTCAAATTCGGACATATTCGGCGTTAATAAGGTCGCACCGCGATAGCGTTCAAAATCCGTCCCTTTCGGATCGATGAGCACCGGCACATTGGCGTTACGGGCAATTTGGATCATTTTTTGTACTTCATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGaa                                                                                                                                                                                                                                      >  1:204663/1‑251 (MQ=255) gAATGACATTTACCCACAACCGCTTCAAATTCGGACATATTCGGCGTTAATAAGGTCGCACCGCGATAGCGTTCAAAATCCGTCCCTTTCGGATCGATGAGCACCGGCACATTGGCGTTACGGGCAATTTGGATCATTTTTTGTACTTCATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGaa                                                                                                                                                                                                                                      >  1:834150/1‑251 (MQ=255) gAATGACATTTACCCACAACCGCTTCAAATTCGGACATATTCGGCGTTAATAAGGTCGCACCGCGATAGCGTTCAAAATCCGTCCCTTTCGGATCGATGAGCACCGGCACATTGGCGTTACGGGCAATTTGGATCATTTTTTGTACTTCATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGaa                                                                                                                                                                                                                                      >  1:309859/1‑251 (MQ=255)       cATTTACCCACAACCGCTTCAAATTCGGACATATTCGGCGTTAATAAGGTCGCACCGCGATAGCGTTCAAAATCCGTCCCTTTCGGATCGATGAGCACCGGCACATTGGCGTTACGGGCAATTTGGATCATTTTTTGTACTTCATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAAtctt                                                                                                                                                                                                                                 >  1:880170/1‑250 (MQ=255)            aCCCACAACCGCTTCAAATTCGGACATATTCGGCGTTAATAAGGTCGCACCGCGATAGCGTTCAAAATCCGTCCCTTTCGGATCGATGAGCACCGGCACATTGGCGTTACGGGCAATTTGGATCATTTTTTGTACTTCATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCaa                                                                                                                                                                                                                           >  1:314827/1‑251 (MQ=255)             cccACAACCGCTTCAAATTCGGACATATTCGGCGTTAATAAGGTCGCACCGCGATAGCGTTCAAAATCCGTCCCTTTCGGATCGATGAGCACCGGCACATTGGCGTTACGGGCAATTTGGATCATTTTTTGTACTTCATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCaaa                                                                                                                                                                                                                          >  1:1307/1‑251 (MQ=255)             cccACAACCGCTTCAAATTCGGACATATTCGGCGTTAATAAGGTCGCACCGCGATAGCGTTCAAAATCCGTCCCTTTCGGATCGATGAGCACCGGCACATTGGCGTTACGGGCAATTTGGATCATTTTTTGTACTTCATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCaaa                                                                                                                                                                                                                          >  1:853376/1‑251 (MQ=255)                       cTTCAAATTCGGACATATTCGGCGTTAATAAGGTCGCACCGCGATAGCGTTCAAAATCCGTCCCTTTCGGATCGATGAGCACCGGCACATTGGCGTTACGGGCAATTTGGATCATTTTTTGTACTTCATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCATAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGt                                                                                                                                                                                                                >  1:450529/1‑251 (MQ=255)                          cAAATTCGGACATATTCGGCGTTAATAAGGTCGCACCGCGATAGCGTTCAAAATCCGTCCCTTTCGGATCGATGAGCACCGGCACATTGGCGTTACGGGCAATTTGGATCATTTTTTGTACTTCATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGtaat                                                                                                                                                                                                             <  1:874896/251‑1 (MQ=255)                            aaTTCGGACATATTCGGCGTTAATAAGGTCGCACCGCGATAGCGTTCAAAATCCGTCCCTTTCGGATCGATGAGCACCGGCACATTGGCGTTACGGGCAATTTGGATCATTTTTTGTACTTCATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGtaataa                                                                                                                                                                                                           <  1:330119/251‑1 (MQ=255)                                      tatTCGGCGTTAATAAGGTCGCACCGCGATAGCGTTCAAAATCCGTCCCTTTCGGATCGATGAGCACCGGCACATTGGCGTTACGGGCAATTTGGATCATTTTTTGTACTTCATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGtaataa                                                                                                                                                                                                           <  1:175201/241‑1 (MQ=255)                                       atTCGGCGTTAATAAGGTCGCACCGCGATAGCGTTCAAAATCCGTCCCTTTCGGATCGATGAGCACCGGCACATTGGCGTTACGGGCAATTTGGATCATTTTTTGTACTTCATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATgg                                                                                                                                                                                                 >  1:635668/1‑250 (MQ=255)                                            gCGTTAATAAGGTCGCACCGCGATAGCGTTCAAAATCCGTCCCTTTCGGATCGATGAGCACCGGCACATTGGCGTTACGGGCAATTTGGATCATTTTTTGTACTTCATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGa                                                                                                                                                                                            >  1:469006/1‑250 (MQ=255)                                                taataaGGTCGCACCGCGATAGCGTTCAAAATCCGTCCCTTTCTGATCGATGATCACCGGCACATTGGCGTTACGGGCAATTTTTATCATTTTTTGTACTTCATTTATTGTGCCTTTGCCGTAATCGGACAAGTTCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGTCGGGACaaaa                                                                                                                                                                                       >  1:550778/1‑251 (MQ=255)                                                             ccGCGATAGCGTTCAAAATCCGTCCCTTTCGGATCGATGAGCACCGGCACATTGGCGTTACGGGCAATTTGGATCATTTTTTGTACTTCATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAAttgt                                                                                                                                                                                                       >  1:332641/1‑222 (MQ=255)                                                             ccGCGATAGCGTTCAAAATCCGTCCCTTTCGGATCGATGAGCACCGGCACATTGGCGTTACGGGCAATTTGGATCATTTTTTGTACTTCATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGt                                                                                                                                                                          >  1:304307/1‑251 (MQ=255)                                                               gcgATAGCGTTCAAAATCCGTCCCTTTCGGATCGATGAGCACCGGCACATTGGCGTTACGGGCAATTTGGATCATTTTTTGTACTTCATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTaa                                                                                                                                                                        >  1:7667/1‑251 (MQ=255)                                                                cgATAGCGTTCAAAATCCGTCCCTTTCGGATCGATGAGCACCGGCACATTGGCGTTACGGGCAATTTGGATCAGTTTTTGTACTTCATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTaa                                                                                                                                                                        <  1:4181/250‑1 (MQ=255)                                                                       gTTCAAAATCCGTCCCTTTCGGATCGATGAGCACCGGCACATTGGCGTTACGGGCAATTTGGATCATTTTTTGTACTTCATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTAATTGTCggg                                                                                                                                                                >  1:505133/1‑251 (MQ=255)                                                                        ttCAAAATCCGTCCCTTTCGGATCGATGAGCACCGGCACATTGGCGTTACGGGCAATTTGGATCATTTTTTGTACTTCATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTaa                                                                                                                                                                        >  1:160299/1‑242 (MQ=255)                                                                        ttCAAAATCCGTCCCTTTCGGATCGATGAGCACCGGCACATTGGCGTTACGGGCAATTTGGATCATTTTTTGTACTTCATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTAATTGTCgggt                                                                                                                                                               >  1:12147/1‑251 (MQ=255)                                                                        ttCAAAATCCGTCCCTTTCGGATCGATGAGCACCGGCACATTGGCGTTACGGGCAATTTGGATCATTTTTTGTACTTCATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTAATTGTCgggg                                                                                                                                                               >  1:648861/1‑250 (MQ=255)                                                                        ttCAAAATCCGTCCCTTTCGGATCGATGAGCACCGGCACATTGGCGTTACGGGCAATTTGGATCATTTTTTGTACTTCATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTAATTGTCggg                                                                                                                                                                <  1:672535/250‑1 (MQ=255)                                                                                          cGGATCGATGAGCACCGGCACATTGGCGTTACGGGCAATTTGGATCATTTTTTGTACTTCATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAGATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTAATTGTCGGGTGGGTAGCAAGCTGTACGa                                                                                                                                             <  1:582346/251‑1 (MQ=255)                                                                                          cGGATCGATGAGCACCGGCACATTGGCGTTACGGGCAATTTGGATCATTTTTTGTACTTCATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTAATTGTCGGGTGGGTAGCAAGCTGTACGa                                                                                                                                             <  1:252109/251‑1 (MQ=255)                                                                                           ggATCGATGAGCACCGGCACATTGGCGTTACGGGCAATTTGGATCATTTTTTGTACTTCATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTAATTGTCGGGTGGGTAGCAAGCTGTACGaa                                                                                                                                            >  1:837157/1‑251 (MQ=255)                                                                                            gATCGATGAGCACCGGCTCATTGGCGTTACGGGCAATTTGGATCATTTTTTGTACTTCATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTAATTGTCGGGTGGGTAGCAAGCTGTACGaa                                                                                                                                            <  1:613294/250‑1 (MQ=255)                                                                                                           gCACATTGGCGTTACGGGCAATTTGGATCATTTTTTGTGCTTCATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTAATTGTCGGGTGGGTAGCAAGCTGTACGAAGTCGCAAGCGAttttt                                                                                                                            >  1:468758/1‑251 (MQ=255)                                                                                                             acaTTGGCGTTACGGGCAATTTGGATCATTTTTTGTACTTCATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGtaat                                                                                                                                                                                                             <  1:465263/168‑1 (MQ=255)                                                                                                                      ttACGGGCAATTTGGATCATTTTTTGTACTTCATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTAATTGTCGGGTGGGTAGCAAGCTGTACGAAGTCGCAAGCGATTTTTTGTTGTTGtaa                                                                                                                 <  1:636692/251‑1 (MQ=255)                                                                                                                        aCGGGCAATTTGGATCATTTTTTGTACTTCATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTAATTGTCGGGTGGGTAGCAAGCTGTACGAAGTCGCAAGCGATTTTTTGTTGTTGtaata                                                                                                               <  1:500709/251‑1 (MQ=255)                                                                                                                          gggCAATTTGGATCATTTTTTGTACTTCATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTAATTGTCGGGTGGGTAGCAAGCTGTACGAAGTCGCAAGCGATTTTTTGTTGTTGTaataat                                                                                                             >  1:36702/1‑251 (MQ=255)                                                                                                                           ggCAATTTGGATCATTTTTTGTACTTCATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGAACAAAATACGCAATTTGGTAATTGTCGGGTGGGTAGCAAGCTGTACGAAGTCGCAAGCGATTTTTTGTTGTTGTAATAATg                                                                                                            >  1:454374/1‑251 (MQ=255)                                                                                                                               aTTTGGATCATTTTTTGTACTTCATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTAATTGTCGGGTGGGTAGCAAGCTGTACGAAGTCGCAAGCGATTTTTTGTTGTTGTAATAATg                                                                                                            >  1:261652/1‑247 (MQ=255)                                                                                                                                     aTCATTTTTTGTACTTCATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTAATTGTCGGGTGGGTAGCAAGCTGTACGAAGTCGCAAGCGATTTTTTGTTGTTGTAATAATGAGGTTAAcgc                                                                                                  <  1:202062/251‑1 (MQ=255)                                                                                                                                     aTCATTTTTTGTACTTCATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTAATTGTCGGGTGGGTAGCAAGCTGTACGAAGTCGCAAGCGATTTTTTGTTGTTGTAATAATGAGGTTAAcgc                                                                                                  <  1:258221/251‑1 (MQ=255)                                                                                                                                         ttttttGTACTTCATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGaa                                                                                                                                                                                                                                      >  1:796347/1‑115 (MQ=255)                                                                                                                                         ttttttGTACTTCATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTAATTGTCGGGTGGGTAGCAAGCTGTACGAAGTCGCAAGCGATTTTTTGTTGTTGtaataa                                                                                                              >  1:442178/1‑235 (MQ=255)                                                                                                                                                    tCATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTAATTGTCGGGTGGGTAGCAAGCTGTACGaa                                                                                                                                            >  1:881699/1‑194 (MQ=255)                                                                                                                                                     cATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTaa                                                                                                                                                                        >  1:741857/1‑165 (MQ=255)                                                                                                                                                     cATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTAATTGTCGGGTGGGTAGCAAGCTGTACGAAGTCGCAAGCGATTTTTTGTTGTTGTAATAATGAGGTTAACGCCGCGCCCGCTTCATcc                                                                                  <  1:784493/251‑1 (MQ=255)                                                                                                                                                     cATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTAATTGTCGGGTGGGTAGCAAGCTGTACGAAGTCGCAAGCGATTTTTTGTTGTTGTAATAATGAGGTTAACGCCGCGCCCGCTTCATcc                                                                                  <  1:368941/251‑1 (MQ=255)                                                                                                                                                     cATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGC‑AATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTAATTGTCGGGTGGGTAGCAAGCTGTACGAAGTCGCAAGCGATTTTTTGTTGTTGTAATAATGAGGTTAAcgc                                                                                                  <  1:71097/234‑1 (MQ=255)                                                                                                                                                              tgCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGGCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTAATTGTCGGGTGGGTAGCAAGCTGTACGAAGTCGCAAGCGATTTTTTGTTGTTGTAATAATGAGGTTAACGCCGCGCCCGCTTCATCCTGACCGGtt                                                                         <  1:432493/251‑1 (MQ=255)                                                                                                                                                                   ttGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTAATTGTCGGGTGGGTAGCAAGCTGTACGAAGTCGCAAGCGATTTTTTGTTGTTGTAATAATGAGGTTAACGCCGCGCCCGCTTCATCCTGACCGGTTAAGcc                                                                    <  1:100487/251‑1 (MQ=255)                                                                                                                                                                     gCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTC‑AAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTAATTGTCGGGTGGGTAGCAAGc                                                                                                                                                    >  1:484712/1‑168 (MQ=255)                                                                                                                                                                     gCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTAACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTAATTGTCGGGTGGGTAGCAAGCTGTACGAAGTCGCAAGCGATTTTTTGTTGTTGTAATAATGAGGTTAACGCCGCGCCCGCTTCATCCTGACCGGTTAAGCCCa                                                                  >  1:367478/1‑251 (MQ=255)                                                                                                                                                                      ccGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTAATTGTCGGGTGGGTAGCAAGCTGTACGAAGTCGCAAGCGATTTTTTGTTGTTGTAATAATGAGGTTAACGCCGCGCCCGCTTCATCCTGACCGGTTAAGCCCAg                                                                 >  1:706127/1‑251 (MQ=255)                                                                                                                                                                        gTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAAttgttg                                                                                                                                                                                                     >  1:751730/1‑117 (MQ=255)                                                                                                                                                                         tAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTAATTGTCGGGTGGGTAGCAAGCTGTACGAAGTCGCAAGCGATTTTTTGTTGTTGTAATAATGAGGTTAACGCCGCGCCCGCTTCATCCTGACCGGTTAAGCCCAGCaa                                                              <  1:803222/251‑1 (MQ=255)                                                                                                                                                                                aCAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTAATTGTCGGGTGGGTAGCAAGCTGTACGAAGTCGCAAGCGATTTTTTGTTGTTGTAATAATGAGGTTAACGCCGCGCCCGCTTCATCCTGACCGGTTAAGCCCAGCAATTGCAc                                                        <  1:77848/250‑1 (MQ=255)                                                                                                                                                                                   aGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTAATTGTCGGGTGGGTAGCAAGCTGTACGAAGTCGCAAGCGATTTTTTGTTGTTGtaataa                                                                                                              <  1:604571/193‑1 (MQ=255)                                                                                                                                                                                    gATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAAt                                                                                                                                                                                                                                    >  1:274964/1‑74 (MQ=255)                                                                                                                                                                                                gTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTAATTGTCGGGTGGGTAGCAAGCTGTACGAAGTCGCAAGCGATTTTTTGTTGTTGTAATAATGAGGTTAACGCCGCGCCCGCTTCATCCTGACCGGTTAAGCCCAGCAATTGCACCGGCACATTTAACGag                                        >  1:492756/1‑249 (MQ=255)                                                                                                                                                                                                              cACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTAATTGTCGGGTGGGTAGCAAGCTGTACGAAGTCGCAAGCGATTTTTTGTTGTTGTAATAATGAGGTTAACGCCGCGCCCGCTTCATCCTGACCGGTTAAGCCCAGCAATTGCACCGGCACATTTAACGAAGCGATGTTCATTGc                          <  1:421536/250‑1 (MQ=255)                                                                                                                                                                                                                  ttCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTAATTGTCGGGTGGGTAGCAAGCTGTACGAAGTCGCAAGCGATTTTTTGTTGTTGTAATAATGAGGTTAACGCCGCGCCCGCtt                                                                                       <  1:901496/185‑1 (MQ=255)                                                                                                                                                                                                                  ttCTAGTTTTTTCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTAATTGTCGGGTGGGTAGCAAGCTGTACGAAGTCGCAAGCGATTTTTTGTTGTTGTAATAATGAGGTTAACGCCGCGCCCGCTTCATcc                                                                                  >  1:159254/1‑190 (MQ=255)                                                                                                                                                                                                                           tttCAGCAAATCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTAATTGTCGGGTGGGTAGCAAGCTGTACGAAGTCGCAAGCGATTTTTTGTTGTTGTAATAATGAGGTTAACGCCGCGCCCGCTTCATCCTGACCGGTTAAGCCCAGCAATTGCACCGGCACATTTAACGAAGCGATGTTCATTGCCACGTTTGCCGCAc            <  1:669001/251‑1 (MQ=255)                                                                                                                                                                                                                                   aaTCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTAATTGTCGGGTGGGTAGCAAGCTGTACGAAGTCGCAAGCGATTTTTTGTTGTTGTAATAATGAGGTTAACGCCGCGCCCGCTTCATCCTGACCGGTTAAGCCCAGCAATTGCACCGGCACATTTAACg                                          >  1:287788/1‑213 (MQ=255)                                                                                                                                                                                                                                    aTCCGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTAATTGTCGGGTGGGTAGCAAGCTGTACGAAGTCGCAAGCGATTTTTTGTTGTTGTAATAATGAGGTTAACGCCGCGCCCGCTTCATCCTGACCGGTTAAGCCCAGCAATTGCACCGGCACATTTAACGAAGCGATGTTCATTGCCACg                      >  1:659094/1‑232 (MQ=255)                                                                                                                                                                                                                                      ccGCACTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTAATTGTCGGGTGGGTAGCAAGCTGTACGAAGTCGCAAGCGATTTTTTGTTGTTGTAATAATGAGGTTAACGCCGCGCCCGCTTCATCCTGACCGGTTAAGCCCAGCAATTGCACCGGCACATTTAACGAAGCGATGTTCATTGCCACGTTTGCCGCACCGCCTGCACg  >  1:72940/1‑250 (MQ=255)                                                                                                                                                                                                                                           cTTGTCACGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTAATTGTCGGGTGGGTAGCAAGCTGTACGAAGTCGCAAGCGATTTTTTGTTGTTGTAATAATGAGGTTAACGCCGCGCCCGCTTCATCCTGACCGGTTAAGccc                                                                   <  1:536690/180‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                              GAATGACATTTACCCACAACCGCTTCAAATTCGGACATATTCGGCGTTAATAAGGTCGCACCGCGATAGCGTTCAAAATCCGTCCCTTTCGGATCGATGAGCACCGGCACATTGGCGTTACGGGCAATTTGGATCATTTTTTGTACTTCATTTAATGTGCCTTTGCCGTAATCGGACAAGATCAAAGCGCCGTAATTTTTGACCGCACTTTCTAGTTTTTTCAGCAAATCCGCACTTGTCATGTTCTGGAAATCTTCTTCAAAATCAAGGCGTAATAATTGTTGATGGCGGGACAAAATACGCAATTTGGTAATTGTCGGGTGGGTAGCAAGCTGTACGAAGTCGCAAGCGATTTTTTGTTGTTGTAATAATGAGGTTAACGCCGCGCCCGCTTCATCCTGACCGGTTAAGCCCAGCAATTGCACCGGCACATTTAACGAAGCGATGTTCATTGCCACGTTTGCCGCACCGCCTGCACG  >  1/1645381‑1645859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑