Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,642,252	T→C	intergenic (+76113/‑21)	unknown → / → unknown	tRNA‑Asn‑GTT/tRNA‑Leu‑CAA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,642,252	0	T	C	100.0%	159.4 / NA	54	intergenic (+76113/‑21)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Leu‑CAA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/19);  total (35/19)

CATAGTGAAAACTCAACTTTTTTTTAATATTTAAAATAATTCTACTCTTATTTTACAAGAAGGGGAATTTTTTCCGGAAAGCCACTACACTAGTAGAAAAGAAGGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTTTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGCACCAATGATAAGTTTCTGAAAAATTAAAGCTGCCGTAAAACGCAGCTTTTTTTGTCACTTGAAGTTAACCCTTCCCTTAAAATTTACCGGAAAAAATGACCGCACTTTTAGCGCCGTTATCAATTATGCGACA  >  1/1642029‑1642487                                                                                                                                                                                                                                |                                                                                                                                                                                                                                            cATAGTGAAAACTCAACTTTTTTTTAATATTTAAAATAATTCTACTCTTATTTTACAAGAAGGGGAATTTTTTCCGGAAAGCCACTACACTAGTAGAAAAGAAAGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCaa                                                                                                                                                                                                                             <  1:901771/240‑1 (MQ=255)                                       atTCTACTCTTATTTTACAAGAAGGGGAATTTTTTCCGGAAAGCCACTACACTAGTAGAAAAGAAAGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGa                                                                                                                                                                                                                                       >  1:48023/1‑192 (MQ=255)                                       atTCTACTCTTATTTTACAAGAAGGGGAATTTTTTCCGGAAAGCCACTACACTAGTAGAAAAGAAAGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATccgc                                                                                                                                                                             >  1:119714/1‑250 (MQ=255)                                             ctctTATTTTACAAGAAGGGGAATTTTTTCCGGAAAGCCACTACACTAGTAGAAAAGAAAGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGaa                                                                                                                                                                      >  1:833617/1‑251 (MQ=255)                                                            agggggAATTTTTGCCGGAAAGCCACTACACTAGTAGAAAAGAAAGTAAGGGAAAAATTTCACATAGATAAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTAGAATACCACTCGTTCTTACGAGGCTTATCAATGCTGCCTGGGTGGCGAAATGGGTAGACGCGGCGAATTCAAATTCCGCCGTTGAATAAACGTGTCGGtt                                                                                                                                                        <  1:576217/248‑1 (MQ=255)                                                                  aaTTTTTTCCGGAAAGCCACTACACTAGTAGAAAAGAAAGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTc                                                                                                                                                  <  1:694399/250‑1 (MQ=255)                                                                                  ccACTACACTAGTAGAAAAGAAAGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCaaaa                                                                                                                                                                                  <  1:371304/202‑1 (MQ=255)                                                                                  ccACTACACTAGTAGAAAAGAAAGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGCACCAAt                                                                                                                                 >  1:881047/1‑251 (MQ=255)                                                                                     ctacactaGTAGAAAAGAAAGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCaa                                                                                                                                                                                                                             <  1:884466/156‑1 (MQ=255)                                                                                     ctacactaGTAGAAAAGAAAGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGCAc                                                                                                                                     >  1:255756/1‑244 (MQ=255)                                                                                        cactaGTAGAAAAGAAAGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCgg                                                                                                                                                                                          >  1:124128/1‑188 (MQ=255)                                                                                             gtagAAAAGAAAGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTa                                                                                                                                                                                                    >  1:467190/1‑173 (MQ=255)                                                                                             gtagAAAAGAAAGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGc                                                                                                                                       <  1:3530/234‑1 (MQ=255)                                                                                             gtagAAAAGAAAGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGc                                                                                                                                       <  1:391722/234‑1 (MQ=255)                                                                                               agaaaagaaaGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGAcc                                                                                                                                             >  1:107852/1‑226 (MQ=255)                                                                                                     gaaagTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGCACCAATGATAAGTTTCTGAAAAAtt                                                                                                              >  1:172227/5‑251 (MQ=255)                                                                                                                    aTTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGCACCAATGATAAGTTTCTGAAAAATTAAAGCTGCCGTaaaa                                                                                               <  1:580528/251‑1 (MQ=255)                                                                                                                              gATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGa                                                                                                                                                                                                                                       >  1:876316/1‑105 (MQ=255)                                                                                                                              gATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTTTCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGCAc                                                                                                                                     <  1:837567/203‑1 (MQ=255)                                                                                                                              gATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGCACCAATGATAAGTTTCTGAAAAATTAAAGCTGCCGTAAAACGCAGCtttt                                                                                     >  1:166784/1‑251 (MQ=255)                                                                                                                                   aaaTTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTc                                                                                                                                                  <  1:644463/185‑1 (MQ=255)                                                                                                                                               gtgtTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGCACCAATGATAAGTTTCTGAAAAATTAAAGCTGCCGTAAAACGCAGCTTTTTTTGTCACTTGAAGTTa                                                                    >  1:838628/1‑251 (MQ=255)                                                                                                                                               gtgtTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGCACCAATGATAAGTTTCTGAAAAATTAAAGCTGCCGTAAAACGCAGCTTTTTTTGTCACTTGAAGTTa                                                                    >  1:646712/1‑251 (MQ=255)                                                                                                                                                    gAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAAATGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCAACCCTAGGCACCAATGATAAGTTTCTGAAAAATTAAAGCTGCCGTAAAACGCAGCTTTTTTTGTCACTTGAAGTTAAcc                                                                 >  1:452892/1‑249 (MQ=255)                                                                                                                                                      aTTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACg                                                                                                                                                                >  1:300465/1‑152 (MQ=255)                                                                                                                                                      aTTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGAccc                                                                                                                                            >  1:248953/1‑172 (MQ=255)                                                                                                                                                      aTTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGCACCAATg                                                                                                                                <  1:750442/184‑1 (MQ=255)                                                                                                                                                      aTTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGCACCAATGATAAGTTTCTGAAAAATTAAAGCTGCCGTAAAACGCAGCttttttt                                                                                  >  1:348889/1‑230 (MQ=255)                                                                                                                                                      aTTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGCACCAATGATAAGTTTCTGAAAAATTAAAGCTGCCGTAAAACGCAGCTTTTTTTGTCACTTGAAGTTAAccctt                                                              >  1:327067/1‑250 (MQ=255)                                                                                                                                                                gTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGCACCAATGATAAGTTTCTGAAAAATTAAAGCTGCCGTAAAACGCAGCTTTTTTTGTCACTTGAAg                                                                       <  1:810687/231‑1 (MQ=255)                                                                                                                                                                gTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGCACCAATGATAAGTTTCTGAAAAATTAAAGCTGCCGTAAAACGCAGCTTTTTTTGTCACTTGAAGTTAACCCTTCCCTTAAAAtt                                                   >  1:794802/1‑251 (MQ=255)                                                                                                                                                                                  gTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGCACCAATGATAAGTTTCTGaa                                                                                                                   >  1:701371/1‑169 (MQ=255)                                                                                                                                                                                  gTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGCACCAATGATAAGTTTCTGAAAAATTAAAGCTGCCGTAAAACGCAGCTTTTTTTGTCACTTGAAGTTAAccc                                                                <  1:309902/220‑1 (MQ=255)                                                                                                                                                                                  gTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGCACCAATGATAAGTTTCTGAAAAATTAAAGCTGCCGTAAAACGCAGCTTTTTTTGTCACTTGAAGTTAACCCTTCCCTTAAAATTTACCGGAAAAAATGACCg                                 >  1:6526/1‑251 (MQ=255)                                                                                                                                                                                           tgcAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGCACCAATGATAAGTTTCTGAAAAATTAAAGCTGCCGTAAAACGCAGCTTTTTTTGTCACTTGAAGTTAACCCTTCCCTTAAAATTTACCGGAAAAAATGACCGCACTTTTAg                        <  1:885306/251‑1 (MQ=255)                                                                                                                                                                                                     aCATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCg                                                                                                                                                                                           >  1:702949/1‑78 (MQ=255)                                                                                                                                                                                                     aCATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGCACCAATGATAAGTTTCTGAAAAATTAAAGCTGCCGTAAAACGCAGCTTTTTTTGTCACTTGAAGTTAACCCTTCCCTTAAAATTTACCGGAAAAAATGACCGCACTTTTAGCGCCGTTAt               >  1:401532/1‑250 (MQ=255)                                                                                                                                                                                                        tAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGCACCAATGATAAGTTTCTGAAAAATTAAAGCTGCCGTAAAACGCAGCTTTTTTTGTCACTTGAAGTTAACCCTTCCCTTAAAATTTACCGGAAAAAATGACCGCACTTTTAGCGCCGTTATCAAt           <  1:137592/251‑1 (MQ=255)                                                                                                                                                                                                          aTCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGCACCAATGATAAGTTTCTGAAAAATTAAAGCTGCCGTAAAACGCAGCTTTTTTTGTCACTTGAAGTTAACCCTTCCCTTAAAATTTACCGGAAAAAATGACCGCACTTTTAGCGCCGTTATCAATTa         >  1:478691/1‑251 (MQ=255)                                                                                                                                                                                                            accTTATAATTCCGCTCGTTCTTACGAAGCTTATCAATGCTGCTTGGGTGGCAAAATTGGTAGACGCAGCGGATTCAAAAACCGCGGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGCACCAATGATAAGTTTCTGAAAAATTAAAGCTGCCGTAAAACGCAGCTTTTTTTGTCACTTGAAGTTAAccc                                                                <  1:137241/193‑1 (MQ=255)                                                                                                                                                                                                              cTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGCACCAATGATAAGTTTCTGAAAAATTAAAGCTGCCGTAAAACGCAGCTTTTTTTGTCACTTGAAGTTAACCCTTCCCTTAAAATTTACCGGAAAAAATGACCGCACTTTTAGCGCCGTTATCAATTATGCg     >  1:551931/1‑251 (MQ=255)                                                                                                                                                                                                               ttATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGTCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGCACCAATGATAAGTTTCTGAAAAATTAAAGCTGCCGTAAAACGCAGCTTTTTTTGTCACTTGAAGTTAACCCTTCCCTTAAAATTTACCGGAAAAAATGACCGCACTTTTAGCGCCGTTATCAATTATGCGa    <  1:440152/251‑1 (MQ=255)                                                                                                                                                                                                               ttATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGCAcc                                                                                                                                    <  1:54437/123‑1 (MQ=255)                                                                                                                                                                                                               ttATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGCACCAATGATAAGTTTCTGAAAAATTAAAGCTGCCGTAAAACGCAGCTTTTTTTGTCACTTGAAGTTAACCCTTCCCTTAAAATTTACCGGAAAAAATGACCGCACTTTTAg                        >  1:271128/1‑231 (MQ=255)                                                                                                                                                                                                               ttATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGCACCAATGATAAGTTTCTGAAAAATTAAAGCTGCCGTAAAACGCAGCTTTTTTTGTCACTTGAAGTTAACCCTTCCCTTAAAATTTACCGGAAAAAATGACCGCACTTTTAGCGCCGTTATCAAt           >  1:879065/1‑244 (MQ=255)                                                                                                                                                                                                               ttATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGCACCAATGATAAGTTTCTGAAAAATTAAAGCTGCCGTAAAACGCAGCTTTTTTTGTCACTTGAAGTTAACCCTTCCCTTAAAATTTACCGGAAAAAATGACCGCACTTTTAGCGCCGTTATCAATTATGc      >  1:94979/1‑249 (MQ=255)                                                                                                                                                                                                               ttATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGCACCAATGATAAGTTTCTGAAAAATTAAAGCTGCCGTAAAACGCAGCTTTTTTTGTCACTTGAAGTTAACCCTTCCCTTAAAATTTACCGGAAAAAATGACCGCACTTTTAGCGCCGTTATCAATTATGCGa    <  1:774367/251‑1 (MQ=255)                                                                                                                                                                                                               ttATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGCACCAATGATAAGTTTCTGAAAAATTAAAGCTGCCGTAAAACGCAGCTTTTTTTGTCACTTGAAGTTAACCCTTCCCTTAAAATTTACCGGAAAAAATGACCGCACTTTTAGCGCCGTTATCAATTATGCGa    >  1:747548/1‑251 (MQ=255)                                                                                                                                                                                                               ttATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGCACCAATGATAAGTTTCTGAAAAATTAAAGCTGCCGTAAAACGCAGCTTTTTTTGTCACTTGAAGTTAACCCTTCCCTTAAAATTTACCGGAAAAAATGACCGCACTTTTAGCGCCGTTATCAATTATGCGa    >  1:842895/1‑251 (MQ=255)                                                                                                                                                                                                               ttATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGCACCAATGATAAGTTTCTGAAAAATTAAAGCTGCCGTAAAACGCAGCTTTTTTTGTCACTTGAAGTTAACCCTTCCCTTAAAATTTACCGGAAAAAATGACCGCACTTTTAGCGCCGTTATCAATTATGCGa    >  1:862610/1‑251 (MQ=255)                                                                                                                                                                                                               ttATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGCACCAATGATAAGTTTCTGAAAAATTAAAGCTGCCGTAAAACGCAGCTTTTTTTGTCACTTGAAGTTAACCCTTCCCTTAAAATTTACCGGAAAAAATGACCGCACTTTTAGCGCCGTTATCAATTATGCGa    >  1:864011/1‑251 (MQ=255)                                                                                                                                                                                                               ttATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGCACCAATGATAAGTTTCTGAAAAATTAAAGCTGCCGTAAAACGCAGCTTTTTTTGTCACTTGAAGTTAACCCTTCCCTTAAAATTTACCGGAAAAAATGACCGCACTTTTAGCGCCGTTATCAATTATGCGa    >  1:515406/1‑251 (MQ=255)                                                                                                                                                                                                               ttATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGCGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGCACCAATGATAAGTTTCTGAAAAATTAAAGCTGCCGTAAAACGCAGCTTTTTTTGTCACTTGAAGTTAACCCTTCCCTTAAAATTTACCGGAAAAAATGACCGCACTTTTAGCGCCGTTATCAATTATGCGa    >  1:685049/1‑251 (MQ=255)                                                                                                                                                                                                                 ataataCCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGCACCAATGATAAGTTTCTGAAAAATTAAAGCTGCCGTAAAACGCAGCTTTTTTTGTCACTTGAAGTTAACCCTTCCCTTAAAATTTACCGGAAAAAATGACCGCACTTTTAGCGCCGTTATCAATTATGCGACa  >  1:313528/1‑251 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                                                                                            CATAGTGAAAACTCAACTTTTTTTTAATATTTAAAATAATTCTACTCTTATTTTACAAGAAGGGGAATTTTTTCCGGAAAGCCACTACACTAGTAGAAAAGAAGGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTTTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGCACCAATGATAAGTTTCTGAAAAATTAAAGCTGCCGTAAAACGCAGCTTTTTTTGTCACTTGAAGTTAACCCTTCCCTTAAAATTTACCGGAAAAAATGACCGCACTTTTAGCGCCGTTATCAATTATGCGACA  >  1/1642029‑1642487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑