Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,642,132	G→A	intergenic (+75993/‑141)	unknown → / → unknown	tRNA‑Asn‑GTT/tRNA‑Leu‑CAA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,642,132	0	G	A	100.0%	102.8 / NA	37	intergenic (+75993/‑141)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Leu‑CAA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/18);  total (19/18)

TAATTAAGCACGTAAAGAGCAACAATAATCCCCAAATACTGGAGGGAATGCCAATGGGAACAAAATGCGCGGTAAGCCCGCCCAAATACAGCATGAAATATAACAGGAGTAACGATCTTGCTAAGTCAAAAACTTTCCGATACATAGTGAAAACTCAACTTTTTTTTAATATTTAAAATAATTCTACTCTTATTTTACAAGAAGGGGAATTTTTTCCGGAAAGCCACTACACTAGTAGAAAAGAAGGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTTTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGCACCAAT  >  1/1641887‑1642360                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                     tAATTAAGCACGTAAAGAGCAACAATAATCCCCAAATACTGGAGGGAATGCCAATGGGAACAAAATGCGCGGTAAGCCCGCCCAAATACAGCATGAAATATAACAGGAGTAACGATCTTGCTAAGTCAAAAACTTTCCGATACATAGTGAAAACTCAACTTTTTTTTAATATTTAAAATAATTCTACTCTTATTTTACAAGAAGGGGAATTTTTTCCGGAAAGCCACTACACTAGTAGAAAAGAAAGTAAg                                                                                                                                                                                                                                 <  1:169840/251‑1 (MQ=255) tAATTAAGCACGTAAAGAGCAACAATAATCCCCAAATACTGGAGGGAATGCCAATGGGAACAAAATGCGCGGTAAGCCCGCCCAAATACAGCATGAAATATAACAGGAGTAACGATCTTGCTAAGTCAAAAACTTTCCGATACATAGTGAAAACTCAACTTTTTTTTAATATTTAAAATAATTCTACTCTTATTTTACAAGAAGGGGAATTTTTTCCGGAAAGCCACTACACTAGTAGAAAAGAAAGTAAg                                                                                                                                                                                                                                 >  1:42143/1‑251 (MQ=255)           cGTAAAGAGCAACAATAATCCCCAAATACTGGAGGGAATGCCAATGGGAACAAAATGCGCGGTAAGCCCGCCCAAATACAGCATGAAATATAACAGGAGTAACGATCTTGCTAAGTCAAAAACTTTCCGATACATAGTGAAAACTCAACTTTTTTTTAATATTTAAAATAATTCTACTCTTATTTTACAAGAAGGGGAATTTTTTCCGGAAAGCCACTACACTAGTAGAAAAGAAAGTAAGGGAAAAAttt                                                                                                                                                                                                                       >  1:53378/1‑251 (MQ=255)                    caacaaTAATCCCCAAATACTGGAGGGAATGCCAATGGGAACAAAATGCGCGGTAAGCCCGCCCAAATACAGCATGAAATATAACAGGAGTAACGATCTTGCTAAGTCAAAAACTTTCCGATACATAGTGAAAACTCAACTTTTTTTTAATATTTAAAATAATTCTACTCTTATTTTACAAGAAGGGGAATTTTTTCCGGAAAGCCACTACACTAGTAGAAAAGAAAGTAAGGGAAAAATTTCACATAGAt                                                                                                                                                                                                              >  1:429729/1‑251 (MQ=255)                                  aaaTACTGGAGGGAATGCCAATGGGAACAAAATGCGCGGTAAGCCCGCCCAAATACAGCATGAAATATAACAGGAGTAACGATCTTGCTAAGTCAAAAACTTTCCGATACATAGTGAAAACTCAACTTTTTTTTAATATTTAAAATAATTCTACTCTTATTTTACAAGAAGGGGAATTTTTTCCGGAAAGCCACTACACTAGTAGAAAAGAAAGTAAGGGAAAAATTTCACATAGATCAAAATTATTg                                                                                                                                                                                                   >  1:541583/1‑248 (MQ=255)                                                 tGCCAATGGGAACAAAATGCGCGGTAAGCCCGCCCAAATACAGCATGAAATATAACAGGAGTAACGATCTTGCTAAGTCAAAAACTTTCCGATACATAGTGAAAACTCAACTTTTTTTTAATATTTAAAATAATTCTACTCTTATTTTACAAGAAGGGGAATTTTTTCCGGAAAGCCACTACACTAGTAGAAAAGAAAGTAAGGGAAAAATTTCACAt                                                                                                                                                                                                                  >  1:712280/1‑218 (MQ=255)                                                             cAAAATGCGCGGTAAGCCCGCCCAAATACAGCATGAAATATAACAGGAGTAACGATCTTGCTAAGTCAAAAACTTTCCGATACATAGTGAAAACTCAACTTTTTTTTAATATTTAAAATAATTCTACTCTTATTTTACAAGAAGGGGAATTTTTTCCGGAAAGCCACTACACTAGTAGAAAAGAAAGTAAGGGAAAAATTTCACAt                                                                                                                                                                                                                  >  1:424324/1‑206 (MQ=255)                                                                              ccgcccAAATACAGCATGAAATATAACAGGAGTAACGATCTTGCTAAGTCAAAAACTTTCCGATACATAGTGAAAACTCAACTTTTTTTTAATATTTAAAATAATTCTACTCTTATTTTACAAGAAGGGGAATTTTTTCCGGAAAGCCACTACACTAGTAGAAAAGAAAGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTttgct                                                                                                                                                    <  1:73320/251‑1 (MQ=255)                                                                                 cccAAATACAGCATGAAATATAACAGGAGTAACGATCTTGCTAAGTCAAAAACTTTCCGATACATAGTGAAAACTCAACTTTTTTTTAATATTTAAAATAATTCTACTCTTATTTTACAAGAAGGGGAATTTTTTCCGGAAAGCCACTACACTAGTAGAAAAGAAAGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTttgcttg                                                                                                                                                  >  1:503676/1‑250 (MQ=255)                                                                                                aaaTATAACAGGAGTAACGATCTTGCTAAGTCAAAAACTTTCCGATACATAGTGAAAACTCAACTTTTTTTTAATATTTAAAATAATTCTACTCTTATTTTACAAGAAGGGGAATTTTTTCCGGAAAGCCACTACACTAGTAGAAAAGAAAGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAAt                                                                                                                                   >  1:256779/1‑250 (MQ=255)                                                                                                           gAGTAACGATCTTGCTAAGTCAAAAACTTTCCGATACATAGTGAAAACTCAACTTTTTTTTAATATTTAAAATAATTCTACTCTTATTTTACAAGAAGGGGAATTTTTTCCGGAAAGCCACTACACTAGTAGAAAAGAAAGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTttgct                                                                                                                                                    >  1:301477/1‑222 (MQ=255)                                                                                                           gAGTAACGATCTTGCTAAGTCAAAAACTTTCCGATACATAGTGAAAACTCAACTTTTTTTTAATATTTAAAATAATTCTACTCTTATTTTACAAGAAGGGGAATTTTTTCCGGAAAGCCACTACACTAGTAGAAAAGAAAGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTataat                                                                                                                         <  1:880347/249‑1 (MQ=255)                                                                                                            aGTAACGATCTTGCTAAGTCAAAAACTTTCCGATACATAGTGAAAACTCAACTTTTTTTTAATATTTAAAATAATTCTACTCTTATTTTACAAGAAGGGGAATTTTTTCCGGAAAGCCACTACACTAGTAGAAAAGAAAGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATAc                                                                                                                       <  1:811780/250‑1 (MQ=255)                                                                                                            aGTAACGATCTTGCTAAGTCAAAAACTTTCCGATACATAGTGAAAACTCAACTTTTTTTTAATATTTAAAATAATTCTACTCTTATTTTACAAGAAGGGGAATTTTTTCCGGAAAGCCACTACACTAGTAGAAAAGAAAGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATAc                                                                                                                       <  1:546687/250‑1 (MQ=255)                                                                                                                   aTCTTGCTAAGTCAAAAACTTTCCGATACATAGTGAAAACTCAACTTTTTTTTAATATTTAAAATAATTCTACTCTTATTTTACAAGAAGGGGAATTTTTTCCGGAAAGCCACTACACTAGTAGAAAAGAAAGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATAc                                                                                                                       <  1:847519/243‑1 (MQ=255)                                                                                                                                               cATAGTGAAAACTCAACTTTTTTTTAATATTTAAAATAATTCTACTCTTATTTTACAAGAAGGGGAATTTTTTCCGGAAAGCCACTACACTAGTAGAAAAGAAAGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCaa                                                                                              <  1:901771/240‑1 (MQ=255)                                                                                                                                                                           atTTAAAATAATTCTACTCTTATTTTACAAGAAGGGGAATTTTTTCCGGAAAGCCACTACACTAGTAGAAAAGAAAGTAAGGGAAAAATTTCACATAGATCAAAATTATTg                                                                                                                                                                                                   <  1:726795/111‑1 (MQ=255)                                                                                                                                                                                aaaTAATTCTACTCTTATTTTACAAGAAGGGGAATTTTTTCCGGAAAGCCACTACACTAGTAGAAAAGAAAGTAAGGGAAAAATTTCACATAGATCAAAat                                                                                                                                                                                                        <  1:856620/101‑1 (MQ=255)                                                                                                                                                                                     atTCTACTCTTATTTTACAAGAAGGGGAATTTTTTCCGGAAAGCCACTACACTAGTAGAAAAGAAAGTAAGGGAAAAATTTCACATAGATCAAAATTATTg                                                                                                                                                                                                   >  1:460532/1‑101 (MQ=255)                                                                                                                                                                                     atTCTACTCTTATTTTACAAGAAGGGGAATTTTTTCCGGAAAGCCACTACACTAGTAGAAAAGAAAGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATAc                                                                                                                       >  1:726341/1‑177 (MQ=255)                                                                                                                                                                                     atTCTACTCTTATTTTACAAGAAGGGGAATTTTTTCCGGAAAGCCACTACACTAGTAGAAAAGAAAGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGa                                                                                                        >  1:48023/1‑192 (MQ=255)                                                                                                                                                                                     atTCTACTCTTATTTTACAAGAAGGGGAATTTTTTCCGGAAAGCCACTACACTAGTAGAAAAGAAAGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATccgc                                              >  1:119714/1‑250 (MQ=255)                                                                                                                                                                                          aCTCTTATTTTACAAGAAGGGGAATTTTTTCCGGAAAGCCACTACACTAGTAGAAAAGAAAGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAAc                                                                                                                                                                        <  1:612934/123‑1 (MQ=255)                                                                                                                                                                                          aCTCTTATTTTACAAGAAGGGGAATTTTTTCCGGAAAGCCACTACACTAGTAGAAAAGAAAGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACaa                                                                                                                                                                      <  1:597130/125‑1 (MQ=255)                                                                                                                                                                                           ctctTATTTTACAAGAAGGGGAATTTTTTCCGGAAAGCCACTACACTAGTAGAAAAGAAAGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGaa                                       >  1:833617/1‑251 (MQ=255)                                                                                                                                                                                            tctTATTTTACAAGAAGGGGAATTTTTTCCGGAAAGCCACTACACTAGTAGAAAAGAAAGTAAGGGAAAAAt                                                                                                                                                                                                                         >  1:484156/1‑72 (MQ=255)                                                                                                                                                                                                          agggggAATTTTTGCCGGAAAGCCACTACACTAGTAGAAAAGAAAGTAAGGGAAAAATTTCACATAGATAAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTAGAATACCACTCGTTCTTACGAGGCTTATCAATGCTGCCTGGGTGGCGAAATGGGTAGACGCGGCGAATTCAAATTCCGCCGTTGAATAAACGTGTCGGtt                         <  1:576217/248‑1 (MQ=255)                                                                                                                                                                                                             gggAATTTTTTCCGGAAAGCCACTACACTAGTAGAAAAGAAAGTAAGGGAAAAATTTCACATAGATCAAAat                                                                                                                                                                                                        <  1:485081/72‑1 (MQ=255)                                                                                                                                                                                                                aaTTTTTTCCGGAAAGCCACTACACTAGTAGAAAAGAAAGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTc                   <  1:694399/250‑1 (MQ=255)                                                                                                                                                                                                                                ccACTACACTAGTAGAAAAGAAAGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCaaaa                                                   <  1:371304/202‑1 (MQ=255)                                                                                                                                                                                                                                ccACTACACTAGTAGAAAAGAAAGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGCACCAAt  >  1:881047/1‑251 (MQ=255)                                                                                                                                                                                                                                   ctacactaGTAGAAAAGAAAGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCaa                                                                                              <  1:884466/156‑1 (MQ=255)                                                                                                                                                                                                                                   ctacactaGTAGAAAAGAAAGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGCAc      >  1:255756/1‑244 (MQ=255)                                                                                                                                                                                                                                      cactaGTAGAAAAGAAAGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCgg                                                           >  1:124128/1‑188 (MQ=255)                                                                                                                                                                                                                                           gtagAAAAGAAAGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTa                                                                     >  1:467190/1‑173 (MQ=255)                                                                                                                                                                                                                                           gtagAAAAGAAAGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGc        <  1:3530/234‑1 (MQ=255)                                                                                                                                                                                                                                           gtagAAAAGAAAGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGc        <  1:391722/234‑1 (MQ=255)                                                                                                                                                                                                                                             agaaaagaaaGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTCTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGAcc              >  1:107852/1‑226 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                     TAATTAAGCACGTAAAGAGCAACAATAATCCCCAAATACTGGAGGGAATGCCAATGGGAACAAAATGCGCGGTAAGCCCGCCCAAATACAGCATGAAATATAACAGGAGTAACGATCTTGCTAAGTCAAAAACTTTCCGATACATAGTGAAAACTCAACTTTTTTTTAATATTTAAAATAATTCTACTCTTATTTTACAAGAAGGGGAATTTTTTCCGGAAAGCCACTACACTAGTAGAAAAGAAGGTAAGGGAAAAATTTCACATAGATCAAAATTATTGAATGTGTTGAATTTTAAACAGTTAAACAAAATTGCAAAGTTTTTGCTTGCAACTGAAACATAATCCCTTATAATACCGCTCGTTTTTACGAAGCTTATCAATGCTGCCTGGGTGGCGAAATTGGTAGACGCAGCGGATTCAAAATCCGCCGTTGAATAAACGTGTCGGTTCGAGTCCGACCCTAGGCACCAAT  >  1/1641887‑1642360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑