Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,616,503	C→T	intergenic (+50364/‑25770)	unknown → / → unknown	tRNA‑Asn‑GTT/tRNA‑Leu‑CAA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,616,503	0	C	T	100.0%	197.5 / NA	69	intergenic (+50364/‑25770)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Leu‑CAA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/32);  total (37/32)

CCCACATGTGTTATTCCGAATTTAACGACATTTTACCGGCAATCGCAGGCTTGGACGCAGATGTGATCACCATCGAAACCTCTCGTTCCGACATGGAATTATTAACGGCATTCGGCGATTTCAAATACCCGAACGACATTGGCCCGGGCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGCCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAACGAAAAAATTACGCGTGGAATTAGCGTAATAAATAGATAAAAAAAGCTGGATCTAGAGAGATTCAGCTTTTGTTTTATGAAAGGAAAAGTGCGGTTGATTTTTAAGGTGTTTTTTTGAATTACCAATATGTTGAATAAGATTAAAAAAGGATTGTATAAAAT  >  1/1616257‑1616739                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                             cccACATGTGTTATTCCGAATTTAACGACATTTTACCGGCAATCGCAGGCTTGGACGCAGATGTGATCACCATCGAAACCTCTCGTTCCGACATGGAATTATTAACGGCATTCGGCGATTTCAAATACCCGAACGACATTGGCCCGGGCGTGTACGACATCCACAGCCCCCGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCaa                                                                                                                                                                                                                                           >  1:444845/1‑250 (MQ=255)   cacaTGTGTTATTCCGAATTTAACGACATTTTACCGGCAATCGCAGGCTTGGACGCAGATGTGATCACCATCGAAACCTCTCGTTCCGACATGGAATTATTAACGGCATTCGGCGATTTCAAATACCCGAACGACATTGGCCCGGGCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAAcc                                                                                                                                                                                                                                         >  1:538663/1‑250 (MQ=255)   cacaTGTGTTATTCCGAATTTAACGACATTTTACCGGCAATCGCAGGCTTGGACGCAGATGTGATCACCATCGAAACCTCTCGTTCCGACATGGAATTATTAACGGCATTCGGCGATTTCAAATACCCGAACGACATTGGCCCGGGCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAAcc                                                                                                                                                                                                                                         >  1:592042/1‑250 (MQ=255)        gtgtTATTCCGAATTTAACGACATTTTACCGGCAATCGCAGGCTTGGACGCAGATGTGATCACCATCGAAACCTCTCGTTCCGACATGGAATTATTAACGGCATTCGGCGATTTCAAATACCCGAACGACATTGGCCCGGGCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGa                                                                                                                                                                                                                                     >  1:282672/1‑249 (MQ=255)        gtgtTATTCCGAATTTAACGACATTTTACCGGCAATCGCAGGCTTGGACGCAGATGTGATCACCATCGAAACCTCTCGTTCCGACATGGAATTATTAACGGCATTCGGCGATTTCAAATACCCGAACGACATTGGCCCGGGCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGAc                                                                                                                                                                                                                                    <  1:26859/250‑1 (MQ=255)         tgtTATTCCGAATTTAACGACATTTTACCGGCAATCGCAGGCTTGGACGCAGATGTGATCACCATCGAAACCTCTCGTTCCGACATGGAATTATTAACGGCATTCGGCGATTTCAAATACCCGAACGACATTGGCCCGGGCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACtg                                                                                                                                                                                                                                  <  1:25906/251‑1 (MQ=255)                   aaTTTAACGACATTTTACCGGCAATCGCAGGCTTGGACGCAGATGTGATCACCATCGAAACCTCTCGTTCCGACATGGAATTATTAACGGCATTCGGCGATTTCAAATACCCGAACGACATTGGCCCGGGCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTaaa                                                                                                                                                                                                                         <  1:763264/250‑1 (MQ=255)                                           tCGCAGGCTTGGACGCAGATGTGATCACCATCGAAACCTCTCGTTCCGACATGGAATTATTAACGGCATTCGGCGATTTCAAATACCCGAACGACATTGGCCCGGGCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCAt                                                                                                                                                                                                 >  1:491948/1‑250 (MQ=255)                                                ggCTTGGACGCAGATGTGATCACCATCGAAACCTCTCGTTCCGACATGGAATTATTAACGGCATTCGGCGATTTCAAATACCCGAACGACATTGGCCCGGGCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATcgccg                                                                                                                                                                                            >  1:355119/1‑250 (MQ=255)                                                     ggACGCAGATGTGATCACCATCGAAACCTCTCGTTCCGACATGGAATTATTAACGGCATTCGGCGATTTCAAATACCCGAACGACATTGGCCCGGGCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACtg                                                                                                                                                                                                                                  >  1:371736/1‑207 (MQ=255)                                                        cGCAGATGTGATCACCATCGAAACCTCTCGTTCCGACATGGAATTATTAACGGCATTCGGCGATTTCAAATACCCGAACGACATTGGCCCGGGCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAg                                                                                                                                                                                   <  1:24322/251‑1 (MQ=255)                                                        cGCAGATGTGATCACCATCGAAACCTCTCGTTCCGACATGGAATTATTAACGGCATTCGGCGATTTCAAATACCCGAACGACATTGGCCCGGGCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAg                                                                                                                                                                                   <  1:306600/251‑1 (MQ=255)                                                        cGCAGATGTGATCACCATCGAAACCTCTCGTTCCGACATGGAATTATTAACGGCATTCGGCGATTTCAAATACCCGAACGACATTGGCCCGGGCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAg                                                                                                                                                                                   <  1:763798/251‑1 (MQ=255)                                                          cAGATGTGATCACCATCGAAACCTCTCGTTCCGACATGGAATTATTAACGGCATTCGGCGATTTCAAATACCCGAACGACATTGGCCCGGGCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAg                                                                                                                                                                                   >  1:848853/1‑249 (MQ=255)                                                            gATGTGATCACCATCGAAACCTCTCGTTCCGACATGGAGTTATTAACGGCATTCGGCGATTTCAAATACCCGAACGACATTGGCCCGGGCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGAt                                                                                                                                                                               >  1:72863/1‑251 (MQ=255)                                                             aTGTGATCACCATCGAAACCTCTCGTTCCGACATGGAATTATTAACGGCATTCGGCGATTTCAAATACCCGAACGACATTGGCCCGGGCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGAtg                                                                                                                                                                              <  1:880250/251‑1 (MQ=255)                                                                 gATCACCATCGAAACCTCTCGTTCCGACATGGAATTATTAACGGCATTCGGCGATTTCAAATACCCGAACGACATTGGCCCGGGCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGAtggtgg                                                                                                                                                                          >  1:66752/1‑251 (MQ=255)                                                                 gATCACCATCGAAACCTCTCGTTCCGACATGGAATTATTAACGGCATTCGGCGATTTCAAATACCCGAACGACATTGGCCCGGGCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGAtggtgg                                                                                                                                                                          >  1:31596/1‑251 (MQ=255)                                                                  aTCACCATCGAAACCTCTCGTTCCGACATGGAATTATTAACGGCATTCGGCGATTTCAAATACCCGAACGACATTGGCCCGGGCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGa                                                                                                                                                                         <  1:24282/251‑1 (MQ=255)                                                                     cccATCGAAACCTCTCGTTCCGACATGGAATTATTAACGGCATTCGGCGATTTCAAATACCCGAACGACATTGGCCCGGGCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGt                                                                                                                                                                      <  1:477873/250‑1 (MQ=255)                                                                       cATCGAAACCTCTCGTTCCGACATGGAATTATTAACGGCATTCGGCGATTTCAAATACCCGAACGACATTGGCCCGGGCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTaa                                                                                                                                                                    >  1:36295/1‑251 (MQ=255)                                                                        aTCGAAACCTCTCGTTCCGACATGGAATTATTAACGGCATTCGGCGATTTCAAATACCCGAACGACATTGGCCCGGGCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAAc                                                                                                                                                                   <  1:303888/251‑1 (MQ=255)                                                                        aTCGAAACCTCTCGTTCCGACATGGAATTATTAACGGCATTCGGCGATTTCAAATACCCGAACGACATTGGCCCGGGCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAAc                                                                                                                                                                   <  1:295906/251‑1 (MQ=255)                                                                                          gACATGGAATTATTAACGGCATTCGGCGATTTCAAATACCCGAACGACATTGGCCCGGGCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGAtggtg                                                                                                                                                                           <  1:240608/225‑1 (MQ=255)                                                                                                 aaTTATTAACGGCATTCGGCGATTTCAAATACCCGAACGACATTGGCCCGGGCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAACGAAAAAATTACGCGTGGAATTAGCg                                                                                                                                          <  1:325871/251‑1 (MQ=255)                                                                                                 aaTTATTAACGGCATTCGGCGATTTCAAATACCCGAACGACATTGGCCCGGGCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAACGAAAAAATTACGCGTGGAATTAGCg                                                                                                                                          <  1:457814/251‑1 (MQ=255)                                                                                                            gCATTCGGCGATTTCAAATACCCGAACGACATTGGCCCGGGCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAACGAAAAAATTACGCGTGGAATTAGCg                                                                                                                                          >  1:654727/1‑240 (MQ=255)                                                                                                                      aTTTCAAATACCCGAACGACATTGGCCCGGGCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAACGAAAAAATTACGCGTGGAATTAGCGTAATAAATAGATAAAAAAAg                                                                                                                      >  1:808199/1‑250 (MQ=255)                                                                                                                      aTTTCAAATACCCGAACGACATTGGCCCGGGCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAACGAAAAAATTACGCGTGGAATTAGCGTAATAAATAGATAAAAAAAGc                                                                                                                     <  1:776764/251‑1 (MQ=255)                                                                                                                             aTACCCGAACGACATTGGCCCGGGCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAACGAAAAAATTACGCGTGGAATTAGCGTAATAAATAGATAAAAAAAGCTGGATCt                                                                                                              <  1:752875/251‑1 (MQ=255)                                                                                                                                cccGAACGACATTGGCCCGGGCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAACGAAAAAATTACGCGTGGAATTAGCGTAATAAATAGATAAAAAAAGCTGGATCTaga                                                                                                           >  1:392630/1‑251 (MQ=255)                                                                                                                                 ccGAACGACATTGGCCCGGGCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAACGAAAAAATTACGCGTGGAATTAGCGTAATAAATAGATAAAAAAAGCTGGATCTagag                                                                                                          <  1:544962/251‑1 (MQ=255)                                                                                                                                 ccGAACGACATTGGCCCGGGCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAACGAAAAAATTACGCGTGGAATTAGCGTAATAAATAGATAAAAAAAGCTGGATCTagag                                                                                                          <  1:388571/251‑1 (MQ=255)                                                                                                                                 ccGAACGACATTGGCCCGGGCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAACGAAAAAATTACGCGTGGAATTAGCGTAATAAATAGATAAAAAAAGCTGGATCTagag                                                                                                          <  1:567960/251‑1 (MQ=255)                                                                                                                                    aaCGACATTGGCCCGGGCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAACGAAAAAATTACGCGTGGAATTAGCGTAATAAATAGATAAAAAAAGCTGGATCTAgagaga                                                                                                       <  1:107235/251‑1 (MQ=255)                                                                                                                                    aaCGACATTGGCCCGGGCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAACGAAAAAATTACGCGTGGAATTAGCGTAATAAATAGATAAAAAAAGCTGGATCTAgagaga                                                                                                       <  1:72216/251‑1 (MQ=255)                                                                                                                                        aCATTGGCCCGGGCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGAtggtg                                                                                                                                                                           >  1:578895/1‑179 (MQ=255)                                                                                                                                        aCATTGGCCCGGGCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAACGAAAAAATTACGCGTGGAATTAGCg                                                                                                                                          >  1:613865/1‑212 (MQ=255)                                                                                                                                        aCATTGGCCCGGGCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAACGAAAAAATTACGCGTGGAATTAGCGTAATAAATAGATAAAAAAAGCTGGATCTagag                                                                                                          >  1:637823/1‑244 (MQ=255)                                                                                                                                        aCATTGGCCCGGGCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAACGAAAAAATTACGCGTGGAATTAGCGTAATAAATAGATAAAAAAAGCTGGATCTAGAGAGATTCa                                                                                                   >  1:381307/1‑251 (MQ=255)                                                                                                                                                   ggCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGCTGTAACGAAAAAATTACGCGTGGAATTAGCGTAATAAATAGATaaaa                                                                                                                          >  1:434385/1‑217 (MQ=255)                                                                                                                                                   ggCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAACGAAAAAATTACGCGTGGAATTAGCGTAATAAATAGATAAAAAAAGCTGGATCTAGAGAGATTCAGCTTTTGtttt                                                                                        >  1:232038/1‑251 (MQ=255)                                                                                                                                                      gtgtACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAACGAAAAAATTACGCGTGGAATTAGCGTAATAAATAGATAAAAAAAGCTGGATCTagag                                                                                                          >  1:51987/1‑230 (MQ=255)                                                                                                                                                       tgtACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAACGAAAAAATTACGCGTGGAATTAGCGTAATAAATAGATAAAAAAAGCTGGATCTAGAGAGATTCAGCTTTTGTTTTATg                                                                                     <  1:884079/250‑1 (MQ=255)                                                                                                                                                       tgtACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAACGAAAAAATTACGCGTGGAATTAGCGTAATAAATAGATAAAAAAAGCTGGATCTAGAGAGATTCAGCTTTTGTTTTATGa                                                                                    <  1:608511/251‑1 (MQ=255)                                                                                                                                                          aCGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAACG‑AAAAATTACGCGTGGAATTAGCGTAATAAATAGATAAAAAAAGCTGGATCTAGAGAGATTCAGCTTTTGTTTTATGAAAgg                                                                                >  1:30855/1‑251 (MQ=255)                                                                                                                                                                tCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAACGAAAAAATTACGCGTGGAATTAGCGTAATAAATAGATAAAAAAAGCTGGATCTagag                                                                                                          <  1:684911/220‑1 (MQ=255)                                                                                                                                                                 ccACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAACGAAAAAATTACGCGTGGaa                                                                                                                                                >  1:619342/1‑181 (MQ=255)                                                                                                                                                                 ccACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAACGAAAAAATTACGCGTGGAATTAGCGTAATAAATAGATAAAAAAAGCTGGATCTAGAGAGATTCAGCTTTTGTTTTATGAAAGGAAAAGt                                                                          >  1:266356/1‑251 (MQ=255)                                                                                                                                                                      gCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAACGAAAAAATTACGCGTGGAATTAGCg                                                                                                                                          <  1:815775/182‑1 (MQ=255)                                                                                                                                                                                  cAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAACGAAAAAATTACGCGTGGAATTAGCGTAATAAATAGATAAAAAAAGCTGGATCTAGAGAGATTCAGCTTTTGTTTTATGAAAGGAAAAGTGCGGTTGATTTTTAAgg                                                         <  1:556316/251‑1 (MQ=255)                                                                                                                                                                                              aaaTCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAACGAAAAAATTACGCGTGGAATTAGCGTAATAAATAGATAAAAAAAGCTGGATCTAGAGAGATTCAGCTTTTGTTTTATGAAAGGAAAAGTGCGGTTGATTTTTAAGgtg                                                       <  1:134612/241‑1 (MQ=255)                                                                                                                                                                                               aaTCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAACGAAAAAATTACGCGTGGAATTAGCGTAATAAATAGATAAAAAAAGCTGGATCTagag                                                                                                          >  1:555208/1‑189 (MQ=255)                                                                                                                                                                                                aTCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGAtggtg                                                                                                                                                                           >  1:750648/1‑123 (MQ=255)                                                                                                                                                                                                aTCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAACGAAAAAATTACGCGTGGAATTAGCGtaataa                                                                                                                                    >  1:382849/1‑162 (MQ=255)                                                                                                                                                                                                          tGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAACGAAAAAATTACGCGTGGAATTAGCGTAATAAATAGATAAAAAAAGCTGGATCTAGAGAGATTCAGCTTTTGTTTTATGAAAGGAAAAGTGCGGTTGATTTTTAAGGTGTTTTTTTGAATTACCAATATGt                                 <  1:751711/251‑1 (MQ=255)                                                                                                                                                                                                                     gCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAACGAAAAAATTACGCGTGGAATTAGCGTAATAAATAGATAAAAAAAGCTGGATCTagag                                                                                                          >  1:467235/1‑167 (MQ=255)                                                                                                                                                                                                                     gCGTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAACGAAAAAATTACGCGTGGAATTAGCGTAATAAATAGATAAAAAAAGCTGGATCTAGAGAGATTCAGCTTTTGTTTTATGAAAGGAAAAGTGCGGTTGATTTTTAAGGTGTTTTTTTGAATTACCAATATGTTGAATAAGAtt                      >  1:235594/1‑251 (MQ=255)                                                                                                                                                                                                                       gTTACAAGTGGTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGCTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTACCGAAAAAATTACGCGTGGAATTAGCGTAATAAATAGATAAAAAAAGCTGGATCTAGAGAGATTCAGCTTTTGTTTTATGAAAGGAAAAGTGCGGTTGATTTTTAAGGAGTTTTTTTGAATTACCAATATGTTGAATAAGATTaa                    >  1:856240/1‑251 (MQ=255)                                                                                                                                                                                                                                ggTACCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAACGAAAAAATTACGCGTGGAATTAGCGTAATAAATAGATAAAAAAAGCTGGATCTAGAGAGATTCAGCTTTTGTTTTATGAAAGGAAAAGTGCGGTTGATTTTTAAGGTGTTTTTTTGAATTACCAATATGTTGAATAAGATTAAAAAAGGAtt           >  1:420144/1‑251 (MQ=255)                                                                                                                                                                                                                                   aCCGAAAGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAACGAAAAAATTACGCGTGGAATTAGCGTAATAAATAGATAAAAAAAGCTGGATCTAGAGAGATTCAGCTTTTGTTTTATGAAAGGAAAAGTGCGGTTGATTTTTAAGGTGTTTTTTTGAATTACCAATATGTTGAATAAGATTAAAAAAGGATTGta        >  1:520047/1‑251 (MQ=255)                                                                                                                                                                                                                                       aaaGAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAACGAAAAAATTACGCGTGGAATTAGCGTAATAAATAGATAAAAAAAGCTGGATCTAGAGAGATTCAGCTTTTGTTTTATGAAAGGAAAAGTGCGGTTGATTTTTAAGGTGTTTTTTTGAATTACCAATATGTTGAATAAGATTAAAAAAGGATTGTATaaa    >  1:822981/1‑251 (MQ=255)                                                                                                                                                                                                                                          gAACGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGt                                                                                                                                                                      <  1:34297/86‑1 (MQ=255)                                                                                                                                                                                                                                             cGCTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAACGAAAAAATTACGCGTGGAATTAGCGTAATAAATAGATAAAAAAAGCTGGATCTAGAGAGATTCAGCt                                                                                                >  1:71030/1‑153 (MQ=255)                                                                                                                                                                                                                                               cTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAACGAAAAAATTACGCGTGGAATTAGCg                                                                                                                                          >  1:205961/1‑109 (MQ=255)                                                                                                                                                                                                                                               cTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAACGAAAAAATTACGCGTGGAATTAGCGtaataa                                                                                                                                    <  1:353765/115‑1 (MQ=255)                                                                                                                                                                                                                                               cTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAACGAAAAAATTACGCGTGGAATTAGCGTAATAAATAGATAAAAAAAGCTGGAt                                                                                                                >  1:837078/1‑135 (MQ=255)                                                                                                                                                                                                                                               cTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAACGAAAAAATTACGCGTGGAATTAGCGTAATAAATAGATAAAAAAAGCTGGATCTAGAGAGATTCAGCTTTTGTTTTATGAAAGGAAAAGTGCGGTTGATTTTTAAGGTGTTTTTTTGAATTACCAatat                                   <  1:18435/212‑1 (MQ=255)                                                                                                                                                                                                                                               cTTATGGGTCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAACGAAAAAATTACGCGTGGAATTAGCGTAATAAATAGATAAAAAAAGCTGGATCTAGAGAGATTCAGCTTTTGTTTTATGAAAGGAAAAGTGCGGTTGATTTTTAAGGTGTTTTTTTGAATTACCAATATGTTGAATAAGATTAAAAAAGGATTGTATAAAAt  <  1:43256/245‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                             CCCACATGTGTTATTCCGAATTTAACGACATTTTACCGGCAATCGCAGGCTTGGACGCAGATGTGATCACCATCGAAACCTCTCGTTCCGACATGGAATTATTAACGGCATTCGGCGATTTCAAATACCCGAACGACATTGGCCCGGGCGTGTACGACATCCACAGCCCACGCGTCCCAACCGCCGCCGAAATCGAACACTTGCTACGCAAAGCGTTACAAGTGGTACCGAAAGAACGCTTATGGGCCAACCCGGACTGTGGTTTAAAAACCCGCGGTTGGCCGGAAACCATCGCCGCATTAAAAGTGATGGTGGATGTAACGAAAAAATTACGCGTGGAATTAGCGTAATAAATAGATAAAAAAAGCTGGATCTAGAGAGATTCAGCTTTTGTTTTATGAAAGGAAAAGTGCGGTTGATTTTTAAGGTGTTTTTTTGAATTACCAATATGTTGAATAAGATTAAAAAAGGATTGTATAAAAT  >  1/1616257‑1616739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑