Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,614,186	T→A	intergenic (+48047/‑28087)	unknown → / → unknown	tRNA‑Asn‑GTT/tRNA‑Leu‑CAA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,614,186	0	T	A	100.0%	131.2 / NA	47	intergenic (+48047/‑28087)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Leu‑CAA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (16/31);  total (16/31)

ATAAACCCGCTTTGCCGCTAAGGAAACGCTACCGGTTTCTTTCAGGGCAAGTAAAGTTTTGAGATGTCTGAGTTCAAGGAAGATCGGTTTCATGAGAAAGATTCATCTAAATTCGAAAAAGAATGAAGCCGTATCATACACGAAAACGACCCGCACTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAATAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCTCCGCAAACTAATAAAATACAGGATCATTAATTTCTTTCATAAACTTTTAGAAAATTATTAATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCG  >  1/1613941‑1614423                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              aTAAACCCGCTTTGCCGCTAAGGAAACGCTACCGGTTTCTTTCAGGGCAAGTAAAGTTTTGAGATGTCTGAGTTCAAGGAAGATCGGTTTCATGAGAAAGATTCATCTAAATTCGAAAAAGAATGAAGCCGTATCATACACGAAAACGACCCGCACTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCg                                                                                                                                                                                                                                            <  1:863252/249‑1 (MQ=255)          cTTTGCCGCTAAGGAAACGCTACCGGTTTCTTTCAGGGCAAGTAAAGTTTTGAGATGTCTGAGTTCAAGGAAGATCGGTTTCATGAGAAAGATTCATCTAAATTCGAAAAAGAATGAAGCCGTATCATACACGAAAACGACCCGCACTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACtaataa                                                                                                                                                                                                                                 <  1:615163/251‑1 (MQ=255)            ttGCCGCTAAGGAAACGCTACCGGTTTCTTTCAGGGCAAGTAAAGTTTTGAGATGTCTGAGTTCAAGGAAGATCGGTTTCATGAGAAAGATTCATCTAAATTCGAAAAAGAATGAAGCCGTATCATACACGAAAACGACCCGCACTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATaaaa                                                                                                                                                                                                                               <  1:467300/251‑1 (MQ=255)               ccGCTAAGGAAACGCTACCGGTTTCTTTCAGGGCAAGTAAAGTTTTGAGATGTCTGAGTTCAAGGAAGATCGGTTTCATGAGAAAGATTCATCTAAATTCGAAAAAGAATGAAGCCGTATCATACACGAAAACGACCCGCACTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATAc                                                                                                                                                                                                                            <  1:204812/251‑1 (MQ=255)               ccGCTAAGGAAACGCTACCGGTTTCTTTCAGGGCAAGTAAAGTTTTGAGATGTCTGAGTTCAAGGAAGATCGGTTTCATGAGAAAGATTCATCTAAATTCGAAAAAGAATGAAGCCGTATCATACACGAAAACGACCCGCACTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATAc                                                                                                                                                                                                                            <  1:225195/251‑1 (MQ=255)                    aaGGAAACGCTACCGGTTTCTTTCAGGGCAAGTAAAGTTTTGAGATGTCTGAGTTCAAGGAAGATCGGTTTCATGAGAAAGATTCATCTAAATTCGAAAAAGAATGAAGCCGTATCATACACGAAAACGACCCGCACTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATAc                                                                                                                                                                                                                            <  1:656907/246‑1 (MQ=255)                    aaGGAAACGCTACCGGTTTCTTTCAGGGCAAGTAAAGTTTTGAGATGTCTGAGTTCAAGGAAGATCGGTTTCATGAGAAAGATTCATCTAAATTCGAAAAAGAATGAAGCCGTATCATACACGAAAACGACCCGCACTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGAt                                                                                                                                                                                                                       <  1:106159/251‑1 (MQ=255)                    aaGGAAACGCTACCGGTTTCTTTCAGGGCAAGTAAAGTTTTGAGATGTCTGAGTTCAAGGAAGATCGGTTTCATGAGAAAGATTCATCTAAATTCGAAAAAGAATGAAGCCGTATCATACACGAAAACGACCCGCACTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGAt                                                                                                                                                                                                                       <  1:126071/251‑1 (MQ=255)                     aGGAAACGCTACCGGTTTCTTTCAGGGCAAGTAAAGTTTTGAGATGTCTGAGTTCAAGGAAGATCGGTTTCATGAGAAAGATTCATCTAAATTCGAAAAAGAATGAAGCCGTATCATACACGAAAACGACCCGCACTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGGAAACTAATAAAATACAGGAt                                                                                                                                                                                                                       >  1:61813/1‑250 (MQ=255)                           cGCTACCGGTTTCTTTCAGGGCAAGTAAAGTTTTGAGATGTCTGAGTTCAAGGAAGATCGGTTTCATGAGAAAGATTCATCTAAATTCGAAAAAGAATGAAGCCGTATCATACACGAAAACGACCCGCACTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGATCATTAAt                                                                                                                                                                                                                <  1:41151/251‑1 (MQ=255)                             cTACCGGTTTCTTTCAGGGCAAGTAAAGTTTTGAGATGTCTGAGTTCAAGGAAGATCGGTTTCATGAGAAAGATTCATCTAAATTCGAAAAAGAATGAAGCCGTATCATACACGAAAACGACCCGCACTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGATCATTa                                                                                                                                                                                                                  <  1:116853/247‑1 (MQ=255)                             cTACCGGTTTCTTTCAGGGCAAGTAAAGTTTTGAGATGTCTGAGTTCAAGGAAGATCGGTTTCATGAGAAAGATTCATCTAAATTCGAAAAAGAATGAAGCCGTATCATACACGAAAACGACCCGCACTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGATCATTAAttt                                                                                                                                                                                                              <  1:803437/251‑1 (MQ=255)                             cTACCGGTTTCTTTCAGGGCAAGTAAAGTTTTGAGATGTCTGAGTTCAAGGAAGATCGGTTTCATGAGAAAGATTCATCTAAATTCGAAAAAGAATGAAGCCGTATCATACACGAAAACGACCCGCACTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGATCATTAAttt                                                                                                                                                                                                              >  1:395334/1‑251 (MQ=255)                                                cAAGTAAAGTTTTGAGATGTCTGAGTTCAAGGAAGATCGGTTTCATGAGAAAGATTCATCTAAATTCGAAAAAGAATGAAGCCGTATCATACACGAAAACGACCCGCACTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGATCATTAATTTCTTTCATAAACTTTTAGaa                                                                                                                                                                                           <  1:185010/251‑1 (MQ=255)                                                                  gTCTGAGTTCAAGGAAGATCGGTTTCATGAGAAAGATTCATCTAAATTCGAAAAAGAATGAAGCCGTATCATACACGAAAACGACCCGCACTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAAt                                                                                                                                                                                                                              <  1:457728/198‑1 (MQ=255)                                                                  gTCTGAGTTCAAGGAAGATCGGTTTCATGAGAAAGATTCATCTAAATTCGAAAAAGAATGAAGCCGTATCATACACGAAAACGACCCGCACTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGATCATTAATTTCTTTCATAAACTTTTAGAAAATTATTAATTTGCataa                                                                                                                                                                         >  1:509600/1‑251 (MQ=255)                                                                     tGAGTTCAAGGAAGATCGGTTTCATGAGAAAGATTCATCTAAATTCGAAAAAGAATGAAGCCGTATCATACACGAAAACGACCCGCACTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGATCATTAATTTCTTTCATAAACTTTTAGAAAATTATTAATTTGCATAATAc                                                                                                                                                                      <  1:75017/251‑1 (MQ=255)                                                                        gTTCAAGGAAGATCGGTTTCATGAGAAAGATTCATCTAAATTCGAAAAAGAATGAAGCCGTATCATACACGAAAACGACCCGCACTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATAc                                                                                                                                                                                                                            <  1:482807/194‑1 (MQ=255)                                                                                           cATGAGAAAGATTCATCTAAATTCGAAAAAGAATGAAGCCGTATCATACACGAAAACGACCCGCACTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGATCATTAATTTCTTTCATAAACTTTTAGAAAATTATTAATTTGCATAATACCCCCGTTTTCGTCATTCTAGcc                                                                                                                                                <  1:746489/251‑1 (MQ=255)                                                                                           cATGAGAAAGATTCATCTAAATTCGAAAAAGAATGAAGCCGTATCATACACGAAAACGACCCGCACTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGATCATTAATTTCTTTCATAAACTTTTAGAAAATTATTAATTTGCATAATACCCCCGTTTTCGTCATTCTAGcc                                                                                                                                                <  1:815456/251‑1 (MQ=255)                                                                                                   aGATTCATCTAAATTCGAAAAAGAATGAAGCCGTATCATACACGAAAACGACCCGCACTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGATCATTAAttt                                                                                                                                                                                                              >  1:111390/1‑181 (MQ=255)                                                                                                             aaaTTCGAAAAAGAATGAAGCCGTATCATACACGAAAACGACCCGCACTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGATCATTAATTTCTTTCATAAACTTTTAGAAAATTATTAATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGc                                                                                                                              <  1:837378/251‑1 (MQ=255)                                                                                                                                 ccGTATCATACACGAAAACGACCCGCACTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGATCATTAAttt                                                                                                                                                                                                              >  1:602508/1‑151 (MQ=255)                                                                                                                                 ccGTATCATACACGAAAACGACCCGCACTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGATCATTAATTTCTTTCATAAACTTTTAGAAAATTATTAATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAacac                                                                                                           <  1:290496/250‑1 (MQ=255)                                                                                                                                     aTCATACACGAAAACGACCCGCACTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGATCATTAATTTCTTTCATAAACTTTTAGAAAATTATTAATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGATGTTTAGCCGTCTAAATCATTTAac                                                                                                             <  1:190254/244‑1 (MQ=255)                                                                                                                                               aaaaCGACCCGTACTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGATCATTAATTTCTTTCATAAACTTTTAGAAAATTATTAATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGa                                                                                                   >  1:401275/1‑244 (MQ=255)                                                                                                                                               aaaaCGACCCGCACTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGATCATTAATTTCTTTCATAAACTTTTAGAAAATTATTAATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTa                                                                                            <  1:218830/251‑1 (MQ=255)                                                                                                                                                   cGACCCGCACTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGATCATTAATTTCTTTCATAAACTTTTAGAAAATTATTAATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAacac                                                                                                           <  1:366302/232‑1 (MQ=255)                                                                                                                                                          cACTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGATCATTa                                                                                                                                                                                                                  >  1:759974/1‑122 (MQ=255)                                                                                                                                                            cTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGATCATTAATTTCTTTCATAAAc                                                                                                                                                                                                   >  1:238718/1‑135 (MQ=255)                                                                                                                                                                 aCGTTACATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAAt                                                                                                                                                                                                                              <  1:680621/103‑1 (MQ=255)                                                                                                                                                                   gTTACATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGAt                                                                                                                                                                                                                       <  1:109150/108‑1 (MQ=255)                                                                                                                                                                      aCATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATAc                                                                                                                                                                                                                            <  1:433914/100‑1 (MQ=255)                                                                                                                                                                      aCATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGAt                                                                                                                                                                                                                       <  1:207304/105‑1 (MQ=255)                                                                                                                                                                      aCATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGATCATTa                                                                                                                                                                                                                  >  1:410925/1‑110 (MQ=255)                                                                                                                                                                      aCATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGATCATTAAttt                                                                                                                                                                                                              <  1:18089/114‑1 (MQ=255)                                                                                                                                                                      aCATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGATCATTAATTTCTTTCATAAACTTTTAGAAAATTATTAATTTGCat                                                                                                                                                                           >  1:86198/1‑149 (MQ=255)                                                                                                                                                                           gaagaaATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGATCATTAATTTCTTTCATAAACTTTTAGaa                                                                                                                                                                                           <  1:317120/128‑1 (MQ=255)                                                                                                                                                                           gaagaaATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGATCATTAATTTCTTTCATAAACTTTTAGAAAATTATTAATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTc                                                                 >  1:142558/1‑250 (MQ=255)                                                                                                                                                                                   ccataaaatccataaaGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGATCATTAATTTCTTTCATAAACTTTTAGAAAATTATTAATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGc                                                                                                                              >  1:406123/1‑181 (MQ=255)                                                                                                                                                                                     ataaaatccataaaGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGATCATTAATTTCTTTCATAAAc                                                                                                                                                                                                   >  1:783299/1‑110 (MQ=255)                                                                                                                                                                                               taaagaaaTCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACt                                                                                                                                                                                                                                      >  1:459720/1‑65 (MQ=255)                                                                                                                                                                                                                aaGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGATCAtt                                                                                                                                                                                                                   <  1:207539/67‑1 (MQ=255)                                                                                                                                                                                                                         cGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGATCATTAATTTCTTTCATAAACTTTTAGAAAATTATTAATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTAt                  <  1:539566/251‑1 (MQ=255)                                                                                                                                                                                                                           tttttAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGATCATTAAt                                                                                                                                                                                                                <  1:165523/59‑1 (MQ=255)                                                                                                                                                                                                                                        tttttATGATTACCACCGCAAACTAATAAAATACAGGATCATTAATTTCTTTCATAAACTTTTAGAAAATTATTAATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAAt    >  1:350790/1‑250 (MQ=255)                                                                                                                                                                                                                                         ttttATGATTACCACCGCAAACTAATAAAATACAGGATCATTAATTTCTTTCATAAACTTTTAGAAAATTATTAATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCg  >  1:369229/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              ATAAACCCGCTTTGCCGCTAAGGAAACGCTACCGGTTTCTTTCAGGGCAAGTAAAGTTTTGAGATGTCTGAGTTCAAGGAAGATCGGTTTCATGAGAAAGATTCATCTAAATTCGAAAAAGAATGAAGCCGTATCATACACGAAAACGACCCGCACTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAATAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCTCCGCAAACTAATAAAATACAGGATCATTAATTTCTTTCATAAACTTTTAGAAAATTATTAATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCG  >  1/1613941‑1614423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑