Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,605,718 T→G intergenic (+39579/‑36555) unknown → / → unknown tRNA‑Asn‑GTT/tRNA‑Leu‑CAA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,605,7180TG100.0% 200.9 / NA 70intergenic (+39579/‑36555)unknown/unknowntRNA‑Asn‑GTT/tRNA‑Leu‑CAA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (34/36);  total (34/36)

ACAAGGCAAAGCCCAGTTTTTCCGCCAACGCATAACATAATGTGCCTTTACCGGCGCCGCTCGGGCCGTCCACCGTAATAATTTTATTCATGATTTTCTCTATTAAAAAGGGATTGAATTTCTGTCGTCATTTTGTCGAACATATCGTAACGTTTGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCTCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGACAATGTAATACTTCCGCCAATACCTTAAACGCATTCACCAACATAAACATCGGGCGCACGCCATGCAATGCTTTTGTTGCCTGACGCACCAAATCCTGCGCTTCATCGCCTTTTGGTCCCTGAATGGAAGCGATC  >  1/1605481‑1605963
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                     
acaaGGCAAAGCCCAGTTTTTCCGCCAACGCATAACATAATGTGCCTTTACCGGCGCCGCTCGGGCCGTCCACCGTAATAATTTTATTCATGATTTTCTCTATTAAAAAGGGATTGAATTTCTGTCGTCATTTTGTCGAACATATCGTAACGTTTGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCt                                                                                                                                                                                                                                           >  1:489781/1‑250 (MQ=255)
          gCCCAGTTTTTCCGCCAACGCATAACATAATGTGCCTTTACCGGCGCCGCTCGGGCCGTCCACCGTAATAATTTTATTCATGATTTTCTCTATTAAAAAGGGATTGAATTTCTGTCGTCATTTTGTCGAACATATCGTAACGTTTGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTg                                                                                                                                                                                                                                 >  1:621333/1‑250 (MQ=255)
                             gCATAACATAATGTGCCTTTACCGGCGCCGCTCGGGCCGTCCACCGTAATAATTTTATTCATGATTTTCTCTATTAAAAAGGGATTGAATTTCTGTCGTCATTTTGTCGAACATATCGTAACGTTTGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTaa                                                                                                                                                                                                             >  1:435628/1‑251 (MQ=255)
                               ataacataaTGTGCCTTTACCGGCGCCGCTCGGGCCGTCCACCGTAATAATTTTATTCATGATTTTCTCTATTAAAAAGGGATTGAATTTCTGTCGTCATTTTGTCGAACATATCGTAACGTTTGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAaac                                                                                                                                                                                                           <  1:610854/251‑1 (MQ=255)
                                 aacataaTGTGCCTTTACCGGCGCCGCTCGGGCCGTCCACCGTAATAATTTTATTCATGATTTTCTCTATTAAAAAGGGATTGAATTTCTGTCGTCATTTTGTCGAACATATCGTAACGTTTGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAaacaa                                                                                                                                                                                                         <  1:240858/251‑1 (MQ=255)
                                  acataaTGTGCCTTTACCGGCGCCGCTCGGGCCGTCCACCGTAATAATTTTATTCATGATTTTCTCTATTAAAAAGGGATTGAATTTCTGTCGTCATTTTGTCGAACATATCGTAACGTTTGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAaacaa                                                                                                                                                                                                         >  1:396173/1‑250 (MQ=255)
                                  acataaTGTGCCTTTACCGGCGCCGCTCGGGCCGTCCACCGTAATAATTTTATTCATGATTTTCTCTATTAAAAAGGGATTGAATTTCTGTCGTCATTTTGTCGAACATATCGTAACGTTTGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAaacaa                                                                                                                                                                                                         >  1:319890/1‑250 (MQ=255)
                                  acataaTGTGCCTTTACCGGCGCCGCTCGGGCCGTCCACCGTAATAATTTTATTCATGATTTTCTCTATTAAAAAGGGATTGAATTTCTGTCGTCATTTTGTCGAACATATCGTAACGTTTGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTCTCCTGCCAAAATTCATCATAGTTAaacaa                                                                                                                                                                                                         >  1:884788/1‑250 (MQ=255)
                                     taaTGTGCCTTTACCGGCGCCGCTCGGGCCGTCCACCGTAATAATTTTATTCATGATTTTCTCTATTAAAAAGGGATTGAATTTCTGTCGTCATTTTGTCGAACATATCGTAACGTTTGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTcatcat                                                                                                                                                                                                                   <  1:475881/237‑1 (MQ=255)
                                         gtgCCTTTACCGGCGCCGCTCGGGCCGTCCACCGTAATAATTTTATTCATGATTTTCTCTATTAAAAAGGGATTGAATTTCTGTCGTCATTTTGTCGAACATATCGTAACGTTTGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCg                                                                                                                                                                                                 >  1:193921/1‑251 (MQ=255)
                                                tACCGGCGCCGCTCGGGCCGTCCACCGTAATAATTTTATTCATGATTTTCTCTATTAAAAAGGGATTGAATTTCTGTCGTCATTTTGTCGAACATATCGTAACGTTTGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCg                                                                                                                                                                                                                                         <  1:477445/204‑1 (MQ=255)
                                                                gCCGTCCACCGTAATAATTTTATTCATGATTTTCTCTATTAAAAAGGGATTGAATTTCTGTCGTCATTTTGTCGAACATATCGTAACGTTTGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTAttt                                                                                                                                                                           <  1:683117/250‑1 (MQ=255)
                                                                        ccGTAATAATTTTATTCATGATTTTCTCTATTAAAAAGGGATTGAATTTCTGTCGTCATTTTGTCGAACATATCGTAACGTTTGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGtt                                                                                                                                                                                                                                       >  1:19986/1‑182 (MQ=255)
                                                                          gTAATAATTTTATTCATGATTTTCTCTATTAAAAAGGGATTGAATTTCTGTCGTCATTTTGTCGAACATATCGTAACGTTTGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACg                                                                                                                                                                  >  1:50723/1‑249 (MQ=255)
                                                                                            aTTTTCTCTATTAAAAAGGGATTGAATTTCTGTCGTCATTTTGTCGAACATATCGTAACGTTTGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCa                                                                                                                                              >  1:498228/1‑251 (MQ=255)
                                                                                            aTTTTCTCTATTAAAAAGGGATTGAATTTCTGTCGTCATTTTGTCGAACATATCGTAACGTTTGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCa                                                                                                                                              >  1:81497/1‑251 (MQ=255)
                                                                                              tttCTCTATTAAAAAGGGATTGAATTTCTGTCGTCATTTTGTCGAACATATCGTAACGTTTGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAg                                                                                                                                            <  1:368520/251‑1 (MQ=255)
                                                                                                 ctctATTAAAAAGGGATTGAATTTCTGTCGTCATTTTGTCGAACATATCGTAACGTTTGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGa                                                                                                                                         >  1:871102/1‑251 (MQ=255)
                                                                                                   ctATTAAAAAGGGATTGAATTTCTGTCGTCATTTTGTCGAACATATCGTAACGTTTGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAAttt                                                                                                                                                                                                   <  1:93028/191‑1 (MQ=255)
                                                                                                     aTTAAAAAGGGATTGAATTTCTGTCGTCATTTTGTCGAACATATCGTAACGTTTGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTAt                                                                                                                                                             <  1:299653/227‑1 (MQ=255)
                                                                                                     aTTAAAAAGGGATTGAATTTCTGTCGTCATTTTGTCGAACATATCGTAACGTTTGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGACAAt                                                                                                                                     >  1:238236/1‑251 (MQ=255)
                                                                                                     aTTAAAAAGGGATTGAATTTCTGTCGTCATTTTGTCGAACATATCGTAACGTTTGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGACAAt                                                                                                                                     >  1:6271/1‑251 (MQ=255)
                                                                                                     aTTAAAAAGGGATTGAATTTCTGTCGTCATTTTGTCGAACATATCGTAACGTTTGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGACAAt                                                                                                                                     >  1:541458/1‑251 (MQ=255)
                                                                                                             gggATTGAATTTCTGTCGTCATTTTGTCGAACATATCGTAACGTTTGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGACAATGTAATa                                                                                                                               >  1:204648/1‑249 (MQ=255)
                                                                                                               gATTGAATTTCTGTCGTCATTTTGTCGAACATATCGTAACGTTTGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGACAATGTAATACtt                                                                                                                            <  1:243833/250‑1 (MQ=255)
                                                                                                                      tttCTGTCGTCATTTTGTCGAACATATCGTAACGTTTGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATtc                                                                                                                                                                                                                        >  1:338183/1‑151 (MQ=255)
                                                                                                                      tttCTGTCGTCATTTTGTCGAACATATCGTAACGTTTGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGACAATGTAATACTTCCGCCAAt                                                                                                                    <  1:51138/251‑1 (MQ=255)
                                                                                                                        tCTGTCGTCATTTTGTCGAACATATCGTAACGTTTGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGACAATGTAATACTTCCGCCAATAc                                                                                                                  <  1:733568/251‑1 (MQ=255)
                                                                                                                          tGTCGTCATTTTGTCGAACATATCGTAACGTTTGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCAGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGACAATGTAATACTTCCGCCAATACCt                                                                                                                <  1:97148/251‑1 (MQ=255)
                                                                                                                           gtcgtcATTTTGTCGAACATATCGTAACGTTTGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGACAATGTAATAc                                                                                                                              >  1:809649/1‑236 (MQ=255)
                                                                                                                               tcATTTTGTCGAACATATCGTAACGTTTGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGACAATGTAATACTTCCGCCAATACCTTAAAc                                                                                                           <  1:372961/251‑1 (MQ=255)
                                                                                                                                 aTTTTGTCGAACATATCGTAACGTTTGCGATACATAGAACGTTTTTTACCCTGGATTTCTTCTAACGGACGACGCTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTCGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCTAACGGTATTTCGcc                                                                                                                                                                       >  1:767482/1‑189 (MQ=255)
                                                                                                                                    ttGTCGAACATATCGTAACGTTTGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGACAATGTAATACTTCCGCCAATACCTTAAACGCAtt                                                                                                      >  1:358816/1‑251 (MQ=255)
                                                                                                                                            cATATCGTAACGTTTGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGACAATGTAATACTTCCGCCAATACCTTAAACGCATTCACCAACa                                                                                              >  1:29975/1‑251 (MQ=255)
                                                                                                                                              tatCGTAACGTTTGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAaac                                                                                                                                                                                                           >  1:304814/1‑140 (MQ=255)
                                                                                                                                              tatCGTAACGTTTGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGACAATGTAATAc                                                                                                                              <  1:880853/217‑1 (MQ=255)
                                                                                                                                               atCGTAACGTTTGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAAcaacaa                                                                                                                                                                                                      >  1:198450/1‑144 (MQ=255)
                                                                                                                                               atCGTAACGTTTGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGACAATGTAATACTTCCGCCAATACCTTAAACGCATTCACCAACATaa                                                                                           >  1:825951/1‑251 (MQ=255)
                                                                                                                                               atCGTAACGTTTGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGACAATGTAATACTTCCGCCAATACCTTAAACGCATTCACCAACATaa                                                                                           >  1:405112/1‑251 (MQ=255)
                                                                                                                                                 cGTAACGTTTGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGACAATGTAATACTTCCGCCAATACCTTAAACGCATTCACCAACATAAAc                                                                                         <  1:206316/251‑1 (MQ=255)
                                                                                                                                                   tAACGTTTGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGACAATGTAATACTTCCGCCAATACCTTAAACGCATTCACCAACATAAACAt                                                                                       <  1:289691/251‑1 (MQ=255)
                                                                                                                                                        tttGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGACAATGTAATACTTCCGCCAATACCTTAAACGCATTCACCAACATAAACATCggg                                                                                   <  1:152687/250‑1 (MQ=255)
                                                                                                                                                        tttGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGACAATGTAATACTTCCGCCAATACCTTAAACGCATTCACCAACATAAACATCggg                                                                                   <  1:688585/250‑1 (MQ=255)
                                                                                                                                                           gCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGc                                                                                                                                                    <  1:443116/182‑1 (MQ=255)
                                                                                                                                                           gCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGACAATg                                                                                                                                    <  1:495076/198‑1 (MQ=255)
                                                                                                                                                                aCATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGACAATGTAATACTTCCGCCAATACCTTAAAc                                                                                                           <  1:250242/218‑1 (MQ=255)
                                                                                                                                                                aCATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGACAATGTAATACTTCCGCCAATACCTTAAACGCATTCACCAACATAAACATCGGGCGCACGCCa                                                                          >  1:97750/1‑251 (MQ=255)
                                                                                                                                                                aCATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTCCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGACAATGTAATACTTCCGCCAATACCTTAAACGCATTCACCAACATAAACATCGGGCGCACGCCa                                                                          >  1:457259/1‑251 (MQ=255)
                                                                                                                                                                     gAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAAGAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGACAATGTAATACTTCCGCCAATACCTTAAACGCATTCACCAACATAAACAt                                                                                       <  1:451597/233‑1 (MQ=255)
                                                                                                                                                                         gTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGc                                                                                                                                                           >  1:599483/1‑161 (MQ=255)
                                                                                                                                                                          ttttttACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACgg                                                                                                                                                                                <  1:706867/139‑1 (MQ=255)
                                                                                                                                                                          ttttttACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACg                                                                                                                                                                                 <  1:728433/138‑1 (MQ=255)
                                                                                                                                                                                  tctGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGACAATGTAATACTTCCGCCAATACCTTAAACGCATTCACCAACATAAACATCGGGCGCACGCCATGCAATGCTTTTGTTGcc                                                        <  1:563333/251‑1 (MQ=255)
                                                                                                                                                                                     ggATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGAc                                                                                                                                        <  1:672580/168‑1 (MQ=255)
                                                                                                                                                                                     ggATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGACAATGTAATACTTCCGCCAATACCTTAAACGCATTCACCAACATAAACAt                                                                                       >  1:695245/1‑217 (MQ=255)
                                                                                                                                                                                          tcttctAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGACAATGTAATAc                                                                                                                              <  1:261685/173‑1 (MQ=255)
                                                                                                                                                                                           cttctAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGACAATGTAATACTTCCGCCAATACCTTAAACGCATTCACCAACATAAACATCGGGCGCACGCCATGCAATGCTTTTGTTGCCTGACGCAc                                                >  1:556202/1‑250 (MQ=255)
                                                                                                                                                                                                 aCGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGACAATGTAATACTTCCGCCAATACCTTAAACGCATTCACCAACATAAACATCGGGCGCACGCCATGCAATGCTTTTGTTGCCTGACGCACCAAATcc                                         <  1:484235/251‑1 (MQ=255)
                                                                                                                                                                                                  cGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGACAATGTAATACTTCCGCCAATACCTTAAACGCATTCACCAACATAAACATCGGGCGCACGCCATGCAATGCTTTTGTTGCCTGACGCACCAAATc                                          >  1:47753/1‑249 (MQ=255)
                                                                                                                                                                                                    gacgacGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGACAATGTAATACTTCCGCCAATACCTTAAACGCATTCACCAACATAAAc                                                                                         >  1:8229/1‑200 (MQ=255)
                                                                                                                                                                                                        gctaaaaaGTGCGGT‑GG‑ATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGACAATGTAATACTTCCGCCAATACCTTAAACGCATTCACCAACATAAACATCggg                                                                                   <  1:83771/194‑1 (MQ=255)
                                                                                                                                                                                                          gTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCgg                                                                                                                                                         <  1:828392/130‑1 (MQ=255)
                                                                                                                                                                                                          gTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGACAATGTAATACTTCCGCCAATACCTTAAACGCATTCACCAACATAAACATCGGGCGCACGCCATGCAATGCTTTTGTTGCCTGACGCACCAAATCCTGCGCTTCa                                >  1:839950/1‑251 (MQ=255)
                                                                                                                                                                                                              aaGTGCGGTCGGAATTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGACAATGTAATACTTCCGCCAATACCTTAAACGCATTCACCAACATAAACATCGGGCGCACGCCATGCAATGCTTTTGTTGCCTGACGCACCAAATCCTGCGCTTCATCGc                            <  1:468111/251‑1 (MQ=255)
                                                                                                                                                                                                                          aaTTTGCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGACAATGTAATACTTCCGCCAATACCTTAAACGCATTCACCAACATAAACATCGGGCGCACGCCATGCAATGCTTTTGTTGCCTGACGCACCAAATCCTGCGCTTCATCGCCTTTTGGTccc                 <  1:271694/250‑1 (MQ=255)
                                                                                                                                                                                                                               gCCAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGACAATGTAATAc                                                                                                                              <  1:615339/136‑1 (MQ=255)
                                                                                                                                                                                                                                ccAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGACAATGTAATACTTCCGCCAATACCTTAAACGCATTCACCAACATAAACATCGGGCGCACGCCATGCAATGCTTTTGTTGCCTGACGCACCAAATCCTGCGCTTCATCGCCTTTTGGTCCCTGAATgg          <  1:85565/251‑1 (MQ=255)
                                                                                                                                                                                                                                 cAATAATTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGACAATGTAATACTTCCGCCAATACCTTAAACGCATTCACCAACATAAACATCGGGCGCACGCCATGCAATGCTTTTGTTGCCTGACGCACCAAATCCTGCGCTTCATCGCCTTTTGGTCCCTGAAt            >  1:371897/1‑248 (MQ=255)
                                                                                                                                                                                                                                   ataatTTTCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGACAATGTAATACTTCCGCCAATACCTTAAACGCATTCACCAACATAAACATCGGGCGCACGCCATGCAATGCTTTTGTTGCCTGACGCACCAAATCCTGCGCTTCATCGCCTTTTGGTCCCTGAATGGagg       <  1:676245/251‑3 (MQ=255)
                                                                                                                                                                                                                                        tttCCGCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGACAATGTAATACTTCCGCCAATACCTTAAACGCATTCACCAACATAAACATCGGGCGCACGCCATGCAATGCTTTTGTTGCCTGACGCACCAAATCCTGCGCTTCATCGCCTTTTGGTCCCTGAATGGAGGCGATc  <  1:200102/251‑1 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                     
ACAAGGCAAAGCCCAGTTTTTCCGCCAACGCATAACATAATGTGCCTTTACCGGCGCCGCTCGGGCCGTCCACCGTAATAATTTTATTCATGATTTTCTCTATTAAAAAGGGATTGAATTTCTGTCGTCATTTTGTCGAACATATCGTAACGTTTGCGATACATAGAACGTTTTTTACTCTGGATTTCTTCTAACGGACGACGTTCAAGTGCGGTCGGAATTTGCCAATAATTTTCCTCTAATTTGCCCTCGTTTTCCTGCCAAAATTCATCATAGTTAAACAACAATTTCGCCGAATCGTTCCAACGGTATTTCGCCTGACGCTTATGCGGAATGCCTTGCAAGCGACAATGTAATACTTCCGCCAATACCTTAAACGCATTCACCAACATAAACATCGGGCGCACGCCATGCAATGCTTTTGTTGCCTGACGCACCAAATCCTGCGCTTCATCGCCTTTTGGTCCCTGAATGGAAGCGATC  >  1/1605481‑1605963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑