Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,603,409	G→A	intergenic (+37270/‑38864)	unknown → / → unknown	tRNA‑Asn‑GTT/tRNA‑Leu‑CAA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,603,409	0	G	A	100.0%	232.8 / NA	80	intergenic (+37270/‑38864)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Leu‑CAA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/45);  total (35/45)

TTTGGAATAGCAACATCTTCTTGCTTATTCACGCTTGCAACTGCCGCGGCTTCTTCAGCTTGATCTTTCGCACGAACAGATAAATGAAC‑GATACGGGCTTTACGATCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAGCGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTGCGTCCACTTGCAATACAACGGCAGCGACTTCGTCACCTTTCTTGTAGTTGCGTACGGCTTCTTCGCCTTGAACATTCCAAGAAATATCGGACAAGTGAACCAAACCGTCGATACCGCCTTCTAAGC  >  1/1603190‑1603652                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    tttGGAATAGCAACATCTTCTTGCTTATTCACGCTTGCAACTGCCGCGGCTTCTTCAGCTTGATCTTTCGCACGAACAGATAAATGAAC‑GATACGGGCTTTACGATCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATAc                                                                                                                                                                                                                      <  1:378864/251‑1 (MQ=255) tttGGAATAGCAACATCTTCTTGCTTATTCACGCTTGCAACTGCCGCGGCTTCTTCAGCTTGATCTTTCGCACGAACAGATAAATGAAC‑GATACGGGCTTTACGATCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATAc                                                                                                                                                                                                                      <  1:345651/251‑1 (MQ=255) tttGGAATAGCAACATCTTCTTGCTTATTCACGCTTGCAACTGCCGCGGCTTCTTCAGCTTGATCTTTCGCACGAACAGATAAATGAAC‑GATACGGGCTTTACGATCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATAc                                                                                                                                                                                                                      <  1:581504/251‑1 (MQ=255)         aGCAACATCTTCTTGCTTATTCACGCTTGCAACTGCCGCGGCTTCTTCAGCTTGATCTTTTGCACGAACAGATAAATGAAC‑GATACGGGCTTTACGATCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCtt                                                                                                                                                                                                              >  1:14620/1‑251 (MQ=255)             aCATCTTCTTGCTTATTCACGCTTGCAACTGCCGCGGCTTCTTCAGCTTGATCTTTCGCACGAACAGATAAATGAAC‑GATACGGGCTTTACGATCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTc                                                                                                                                                                                                                                                 <  1:609414/212‑1 (MQ=255)                 cttcttGCTTATTCACGCTTGCAACTGCCGCGGCTTCTTCAGCTTGATCTTTCGCACGAACAGATAAATGAAC‑GATACGGGCTTTACGATCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAAt                                                                                                                                                                                                                        <  1:129723/233‑1 (MQ=255)                 cttcttGCTTATTCACGCTTGCAACTGCCGCGGCTTCTTCAGCTTGATCTTTCGCACGAACAGATAAATGAAC‑GATACGGGCTTTACGATCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTttgttg                                                                                                                                                                                                      >  1:50541/1‑251 (MQ=255)                          tATTCACGCTTGCAACTGCCGCGGCTTCTTCAGCTTGATCTTTCGCACGAACAGATAAATGAACGGATACGGGCTTTACGATCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAAc                                                                                                                                                                                              <  1:560588/251‑1 (MQ=255)                           aTTCACGCTTGCAACTGCCGCGGCTTCTTCAGCTTGATCTTTCGCACGAACAGATAAATGAAC‑GATACGGGCTTTACGATCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGa                                                                                                                                                                                            <  1:687964/251‑1 (MQ=255)                             tCACGCTTGCAACTGCCGCGGCTTCTTCAGCTTGATCTTTCGCACGAACAGATAAATGAAC‑GATACGGGCTTTACGATCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAg                                                                                                                                                                                          <  1:484137/251‑1 (MQ=255)                                   ttGCAACTGCCGCGGCTTCTTCAGCTTGATCTTTCGCACGAACAGATAAATGAAC‑GATACGGGCTTTACGATCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTg                                                                                                                                                                                    <  1:621005/251‑1 (MQ=255)                                      cAACTGCCGCGGCTTCTTCAGCTTGATCTTTCGCACGAACAGATAAATGAAC‑GATACGGGCTTTACGATCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGaa                                                                                                                                                                                  <  1:498692/250‑1 (MQ=255)                                            ccGCGGCTTCTTCAGCTTGATCTTTCGCACGAACAGATAAATGAAC‑GATACGGGCTTTACGATCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGAtc                                                                                                                                                                            <  1:739459/250‑1 (MQ=255)                                            ccGCGGCTTCTTCAGCTTGATCTTTCGCACGAACAGATAAATGAAC‑GATACGGGCTTTACGATCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGAtc                                                                                                                                                                            <  1:492156/250‑1 (MQ=255)                                                  cttcttCAGCTTGATCTTTCGCACGAACAGATAAATGAAC‑GATACGGGCTTTACGATCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCttcttc                                                                                                                                                                      <  1:553817/250‑1 (MQ=255)                                                     cttcAGCTTGATCTTTCGCACGAACAGATAAATGAAC‑GATACGGGCTTTACGATCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAgt                                                                                                                                                                  >  1:677368/1‑251 (MQ=255)                                                          gCTTGATCTTTCGCACGAACAGATAAATGAAC‑GATACGGGCTTTACGATCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGttt                                                                                                                                                             >  1:414347/1‑251 (MQ=255)                                                           cTTGATCTTTCGCACGAACAGATAAATGAAC‑GATACGGGCTTTACGATCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTg                                                                                                                                                            <  1:46764/251‑1 (MQ=255)                                                             tGATCTTTCGCACGAACAGATAAATGAAC‑GATACGGGCTTTACGATCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGAt                                                                                                                                                          <  1:532079/251‑1 (MQ=255)                                                               aTCTTTCGCACGAACAGATAAATGAAC‑GATACGGGCTTTACGATCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATAc                                                                                                                                                        <  1:490744/251‑1 (MQ=255)                                                                       cACGAACAGATAAATGAAC‑GATACGGGCTTTACGATCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGa                                                                                                                                                 <  1:879290/250‑1 (MQ=255)                                                                           aaCAGATAAATGAAC‑GATACGGGCTTTACGATCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATa                                                                                                                                             <  1:198372/250‑1 (MQ=255)                                                                               gATAAATGAAC‑GATACGGGCTTTACGATCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATa                                                                                                                                             <  1:183628/246‑1 (MQ=255)                                                                                       aaC‑GATACGGGCTTTACGATCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCTGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTg                                                                                                                                <  1:447411/251‑1 (MQ=255)                                                                                       aaC‑GATACGGGCTTTACGATCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCTGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTg                                                                                                                                <  1:440218/251‑1 (MQ=255)                                                                                       aaC‑GATACGGGCTTTACGATCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTg                                                                                                                                <  1:329471/251‑1 (MQ=255)                                                                                       aaC‑GATACGGGCTTTACGATCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTg                                                                                                                                <  1:123456/251‑1 (MQ=255)                                                                                         c‑GATACGGGCTTTACGATCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGt                                                                                                                                          <  1:719300/239‑1 (MQ=255)                                                                                            aTACGGGCTTTACGATCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTGCGt                                                                                                                             >  1:631399/1‑250 (MQ=255)                                                                                            aTACGGGCTTTACGATCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCCAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTGCGt                                                                                                                             >  1:635680/1‑250 (MQ=255)                                                                                                 ggCTTTACGATCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTGCGTCCACtt                                                                                                                       >  1:126199/1‑251 (MQ=255)                                                                                                 ggCTTTACGATCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTGCGTCCACt                                                                                                                        <  1:828119/250‑1 (MQ=255)                                                                                                   cTTTACGATCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATAc                                                                                                                                                                                                                      >  1:299048/1‑154 (MQ=255)                                                                                                     ttACGATCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTGCGTCCACTTGCaa                                                                                                                   <  1:867740/251‑1 (MQ=255)                                                                                                      tACGATCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTGCGTCCACTTGCAAt                                                                                                                  <  1:127158/251‑1 (MQ=255)                                                                                                        cGATCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTGCGTCCACTTGCAATAc                                                                                                                <  1:560555/251‑1 (MQ=255)                                                                                                        cGATCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTGCGTCCACTTGCAATAc                                                                                                                <  1:235186/251‑1 (MQ=255)                                                                                                        cGATCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTGCGTCCACTTGCAATAc                                                                                                                <  1:154314/251‑1 (MQ=255)                                                                                                          aTCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTc                                                                                                                                                                                                                                                 >  1:823399/1‑120 (MQ=255)                                                                                                          aTCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAAt                                                                                                                                                                                                                        <  1:318155/145‑1 (MQ=255)                                                                                                          aTCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGTCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTGCGTCCACTTGCAATACaa                                                                                                              >  1:805048/1‑251 (MQ=255)                                                                                                          aTCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAgt                                                                                                                                                                  >  1:111516/1‑199 (MQ=255)                                                                                                          aTCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAg                                                                                                                                                                   <  1:136863/198‑1 (MQ=255)                                                                                                          aTCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGttt                                                                                                                                                             <  1:743897/204‑1 (MQ=255)                                                                                                          aTCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCAc                                                                                                                                  >  1:130254/1‑231 (MQ=255)                                                                                                          aTCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCAc                                                                                                                                  <  1:437628/231‑1 (MQ=255)                                                                                                          aTCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTGCGTCCACTTGCAATAc                                                                                                                >  1:314112/1‑249 (MQ=255)                                                                                                          aTCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTGCGTCCACTTGCAATACaa                                                                                                              >  1:232880/1‑251 (MQ=255)                                                                                                          aTCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTGCGTCCACTTGCAATACaa                                                                                                              >  1:704030/1‑251 (MQ=255)                                                                                                          aTCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGATCTTTCACTGCGTCCACTTGCAATACaa                                                                                                              >  1:625064/1‑251 (MQ=255)                                                                                                            cTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAAt                                                                                                                                                                                                                        >  1:86506/1‑143 (MQ=255)                                                                                                            cTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTGCGTCCACTTGCAATACAACg                                                                                                            >  1:459722/1‑251 (MQ=255)                                                                                                            cTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTGCGTCCACTTGCAATACAACg                                                                                                            >  1:611393/1‑251 (MQ=255)                                                                                                                   tgtgTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTttgttg                                                                                                                                                                                                      >  1:121985/1‑154 (MQ=255)                                                                                                                    gtgtATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTGCGTCCACTTGCAATACAACGGCAGCGa                                                                                                     >  1:556120/1‑250 (MQ=255)                                                                                                                            cGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTGCGTCCACTTGCAATAc                                                                                                                >  1:161436/1‑231 (MQ=255)                                                                                                                                    caacaTCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCGAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTGCGTCCACTTGCAATACAACGGCAGCGACTTCGTCACCTTTCTTg                                                                                    <  1:479267/251‑1 (MQ=255)                                                                                                                                          cGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTGCGTCCACTTGCAATACAACGGCAGCGACTTCGTCACCTTTCTTGTAGTTg                                                                              <  1:288095/251‑1 (MQ=255)                                                                                                                                                cAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTGCGTCCACTTGCAATACAACGGCAGCGACTTCGTCACCTTTCTTGTAGTTGCGTAc                                                                         <  1:570975/250‑1 (MQ=255)                                                                                                                                                      cTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTGCGTCCACTTGCAATACAACGGCAGCGACTTCGTCACCTTTCTTGTAGTTGCGTACGGctt                                                                    >  1:461321/1‑249 (MQ=255)                                                                                                                                                             agttaAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTGCGTCCACTTGCAATACAACGGCAGCGACTTCGTCACCTTTCTTGTAGTTGCGTACGGCTTCTTCGCCtt                                                           >  1:243417/1‑251 (MQ=255)                                                                                                                                                                       gCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTGCGTCCACTTGCAATACAACGGCAGCGACTTCGTCACCTTTCTTGTAGTTGCGTACGGCTTCTTCGCCTTGAACATTCCa                                                 >  1:3745/1‑251 (MQ=255)                                                                                                                                                                               gATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTGCGTCCACTTGCAAt                                                                                                                  <  1:697565/178‑1 (MQ=255)                                                                                                                                                                               gATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTGCGTCCACTTGCAATACAACGGCAGCGACTTCGTCACCTTTCTTGTAGTTGCGTACGGCTTCTTCGCCTTGAACATTCCAAGAAatat                                         >  1:89575/1‑251 (MQ=255)                                                                                                                                                                               gATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTGCGTCCACTTGCAATACAACGGCAGCGACTTCGTCACCTTTCTTGTAGTTGCGTACGGCTTCTTCGCCTTGAACATTCCAAGAAatat                                         >  1:298729/1‑251 (MQ=255)                                                                                                                                                                               gATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTGCGTCCACTTGCAATACAACGGCAGCGACTTCGTCACCTTTCTTGTAGTTGCGTACGGCTTCTTCGCCTTGAACATTCCAAGAAatat                                         >  1:685048/1‑251 (MQ=255)                                                                                                                                                                               gATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTGCGTCCACTTGCAATACAACGGCAGCGACTTCGTCACCTTTCTTGTAGTTGCGTACGGCTTCTTCGCCTTGAACATTCCAAGAAatat                                         >  1:352219/1‑251 (MQ=255)                                                                                                                                                                                     aCCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTttg                                                                                                                                                                                                         <  1:738099/85‑1 (MQ=255)                                                                                                                                                                                     aCCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTGCGTCCACTTGCAATACAACGGCAGCGACTTCGTCACCTTTCTTGTAGTTGCGTACGGctt                                                                    <  1:470382/218‑1 (MQ=255)                                                                                                                                                                                     aCCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTGCGTCCACTTGCAATACAACGGCAGCGACTTCGTCACCTTTCTTGTAGTTGCGTACGGCTTCTTCGCCTTGAACATTCCAAGAAATATCGGACa                                   >  1:887201/1‑251 (MQ=255)                                                                                                                                                                                       cTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTGCGTCCACTTGCAATACAACGGCAGCGACTTCGTCACCTTTCTTGTAGTTGCGTACGGCTTCTTCGCCTTGAACATTCCAAGAAATATCGGACAAg                                 <  1:647044/251‑1 (MQ=255)                                                                                                                                                                                                  ccgTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTGCGTCCACTTGCAATACAACGGCAGCGACTTCGTCACCTTTCTTGTAGTTGCGTACGGCTTCTTCGCCTTGAACATTCCAAGAAATATCGGACAAGTGAACCAAAc                       <  1:157777/250‑1 (MQ=255)                                                                                                                                                                                                    gTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTGCGTc                                                                                                                            >  1:566841/1‑147 (MQ=255)                                                                                                                                                                                                    gTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTGCGTCCACTTGCaa                                                                                                                   <  1:631080/156‑1 (MQ=255)                                                                                                                                                                                                    gTCTAATTCAACTTTAGCGCCTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTGCGTCCACTTGCAATACAACGGCAGCGACTTCGTCACCTTTCTTGTAGTTGCGTACGGCTTCTTCGCCTTGAACATTCCAAGAAATATCGGACAAGTGAACCAAACCGt                    >  1:43136/1‑251 (MQ=255)                                                                                                                                                                                                                       ccTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTGCGTCCACTTGCAATACAACGGCAGCGACTTCGTCACCTTTCTTGTAGTTGCGTACGGCTTCTTCGCCTTGAACATTCCAAGAAATATCGGACAAGTGAAcc                           >  1:866987/1‑225 (MQ=255)                                                                                                                                                                                                                       ccTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTGCGTCCACTTGCAATACAACGGCAGCGACTTCGTCACCTTTCTTGTAGTTGCGTACGGCTTCTTCGCCTTGAACATTCCAAGAAATATCGGACAAGTGAACCAAACCGTCGATACCGCCTTCTAAGc  <  1:376226/250‑1 (MQ=255)                                                                                                                                                                                                                       ccTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTGCGTCCACTTGCAATACAACGGCAGCGACTTCGTCACCTTTCTTGTAGTTGCGTACGGCTTCTTCGCCTTGAACATTCCAAGAAATATCGGACAAGTGAACCAAACCGTCGATACCGCCTTCTAAGc  >  1:530039/1‑250 (MQ=255)                                                                                                                                                                                                                       ccTTTAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTGCGTCCACTTGCAATACAACGGCAGCGACTTCGTCACCTTTCTTGTAGTTGCGTACGGCTTCTTCGCCTTGAACATTCCAAGAAATATCGGACAAGTGAACCAAACCGTCGATACCGCCTTCTAAGc  <  1:193294/250‑1 (MQ=255)                                                                                                                                                                                                                         tttAACGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTGCGTCCACTTGCAATACAACGGCAGCGACTTCGTCACCTTTCTTGTAGTTGCGTACGGCTTCTTCGCCTTGAACATTCCAAGAAATATCGGACAAGTGAACCAAACCGTCGATAc              >  1:874281/1‑236 (MQ=255)                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    TTTGGAATAGCAACATCTTCTTGCTTATTCACGCTTGCAACTGCCGCGGCTTCTTCAGCTTGATCTTTCGCACGAACAGATAAATGAAC‑GATACGGGCTTTACGATCTACGCCTGTGTATTTCGCTTCAACAACATCGCCGGCAACCACTTCGTTAGTTAAATCAGCCGCGCGGATATAACCTTCAACACCGCCGTCTAATTCAACTTTAGCGCCTTTAGCGTCTGCTTCAACAACTTTAGCGTTTAATACTGCGCCTTTTTTGTTGATTGCAACGAAGTTATTGAACGGATCTTCTTCAAGTTGTTTGATACCTAAAGAAATACGTTCTTTCACTGCGTCCACTTGCAATACAACGGCAGCGACTTCGTCACCTTTCTTGTAGTTGCGTACGGCTTCTTCGCCTTGAACATTCCAAGAAATATCGGACAAGTGAACCAAACCGTCGATACCGCCTTCTAAGC  >  1/1603190‑1603652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑