Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,582,399	G→A	intergenic (+16260/‑59874)	unknown → / → unknown	tRNA‑Asn‑GTT/tRNA‑Leu‑CAA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,582,399	0	G	A	100.0%	220.7 / NA	74	intergenic (+16260/‑59874)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Leu‑CAA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (42/32);  total (42/32)

GCCAGAGGCAGACTCATGGCAGCACCGACTTTCGGAATGCGGATCCAGGGTTCCGGTTTTGAACCGGCGCAATAAAAGTGCGGTGGAAATTTTGAGAGAATTTCAACGCCCGATTGCTGCTGTAAATAAGCAAAGGCAGAGGCATAAAGTGCGGTCGGAAATTGCGCTGAATATTGCGTCGCCAGATGTTGCGTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGGAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAGTCAGCTTGGAAGCACCGGAAAAATGGATTTTGACCGGCAAAAAAATCTCGATGCTGTAAAAAAGATAGGCAGCAACGCCTACTGCGACTATGCCAAATAAAGCG  >  1/1582156‑1582644                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      gCCAGAGGCAGACTCATGGCAGCACCGACTTTCGGAATGCGGATCCAGGGTTCCGGTTTTGAACCGGCGCAATAAAAGTGCGGTGGAAATTTTGAGAGAATTTCAACGCCCGATTGCTGCTGTAAATAAGCAAAGGCAGAGGCATAAAGTGCGGTCGGAAATTGCGCTGGATATTGCGTCGCCAGATGTTGCGTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAgcg                                                                                                                                                                                                                                                 >  1:441764/1‑250 (MQ=255)          aGACTCATGGCAGCACCGACTTTCGGAATGCGGATCCAGGGTTCCGGTTTTGAACCGGCGCAATAAAAGTGCGGTGGAAATTTTGAGAGAATTTCAACGCCCGATTGCTGCTGTAAATAAGCAAAGGCAGAGGCATAAAGTGCGGTCGGAAATTGCGCTGAATATTGCGTCGCCAGATGTTGCGTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCa                                                                                                                                                                                                                                       >  1:854994/1‑251 (MQ=255)                                        cGGATCCAGGGTTCCGGTTTTGAACCGGCGCAATAAAAGTGCGGTGGAAATTTTGAGAGAATTTCAACGCCCGATTGCTGCTGTAAATAAGCAAAGGCAGAGGCATAAAGTGCGGTCGGAAATTGCGCTGAATATTGCGTCGCCAGATGTTGCGTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTcc                                                                                                                                                                                                          <  1:838123/250‑1 (MQ=255)                                           aTCCAGGGTTCCGGTTTTGAACCGGCGCAATAAAAGTGCGGTGGAAATTTTGAGAGAATTTCAACGCCCGATTGCTGCTGTAAATAAGCAAAGGCAGAGGCATAAAGTGCGGTCGGAAATTGCGCTGAATATTGCGTCGCCAGATGTTGCGTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCaaa                                                                                                                                                                                                       <  1:498320/250‑1 (MQ=255)                                            tCCAGGGTTCCGGTTTTGAACCGGCGCAATAAAAGTGCGGTGGAAATTTTGAGAGAATTTCAACGCCCGATTGCTGCTGTAAATAAGCAAAGGCAGAGGCATAAAGTGCGGTCGGAAATTGCGCTGAATATTGCGTCGCCAGATGTTGCGTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACg                                                                                                                                                                                                     <  1:247855/251‑1 (MQ=255)                                             ccAGGGTTCCGGTTTTGAACCGGCGCAATAAAAGTGCGGTGGAAATTTTGAGAGAATTTCAACGCCCGATTGCTGCTGTAAATAAGCAAAGGCAGAGGCATAAAGTGCGGTCGGAAATTGCGCTGAATATTGCGTCGCCAGATGTTGCGTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGa                                                                                                                                                                                                    >  1:476679/1‑251 (MQ=255)                                                   ttCCGGTTTTGAACCGGCGCAATAAAAGTGCGGTGGAAATTTTGAGAGAATTTCAACGCCCGATTGCTGCTGTAAATAAGCAAAGGCAGAGGCATAAAGTGCGGTCGGAAATTGCGCTGAATATTGCGTCGCCAGATGTTGCGTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAAc                                                                                                                                                                                              <  1:141798/251‑1 (MQ=255)                                                             gAACCGGCGCAATAAAAGTGCGGTGGAAATTTTGAGAGAATTTCAACGCCCGATTGCTGCTGTAAATAAGCAAAGGCAGAGGCATAAAGTGCGGTCGGAAATTGCGCTGAATATTGCGTCGCCAGATGTTGCGTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGcc                                                                                                                                                                                                                                               <  1:335057/192‑1 (MQ=255)                                                             gAACCGGCGCAATAAAAGTGCGGTGGAAATTTTGAGAGAATTTCAACGCCCGATTGCTGCTGTAAATAAGCAAAGGCAGAGGCATAAAGTGCGGTCGGAAATTGCGCTGAATATTGCGTCGCCAGATGTTGCGTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTAtt                                                                                                                                                                                    >  1:72961/1‑251 (MQ=255)                                                                   gcgcAATAAAAGTGCGGTGGAAATTTTGAGAGAATTTCAACGCCCGATTGCTGCTGTAAATAAGCAAAGGCAGAGGCATAAAGTGCGGTCGGAAATTGCGCTGAATATTGCGTCGCCAGATGTTGCGTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTtgtg                                                                                                                                                                               <  1:431936/250‑1 (MQ=255)                                                                        aTAAAAGTGCGGTGGAAATTTTGAGAGAATTTCAACGCCCGATTGCTGCTGTAAATAAGCAAAGGCAGAGGCATAAAGTGCGGTCGGAAATTGCGCTGAATATTGCGTCGCCAGATGTTGCGTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTtg                                                                                                                                                                                 <  1:824243/243‑1 (MQ=255)                                                                                   gTGGAAATTTTGAGAGAATTTCAACGCCCGATTGCTGCTGTAAATAAGCAAAGGCAGAGGCATAAAGTGCGGTCGGAAATTGCGCTGAATATTGCGTCGCCAGATGTTGCGTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGtttt                                                                                                                                                              >  1:728712/1‑251 (MQ=255)                                                                                                  gaATTTCAACGCCCGATTGCTGCTGTAAATAAGCAAAGGCAGAGGCATAAAGTGCGGTCGGAAATTGCGCTGAATATTGCGTCGCCAGATGTTGCGTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTaa                                                                                                                                               >  1:351475/1‑251 (MQ=255)                                                                                                   aaTTTCAACGCCCGATTGCTGCTGTAAATAAGCAAAGGCAGAGGCATAAAGTGCGGTCGGAAATTGCGCTGAATATTGCGTCGCCAGATGTTGCGTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAAc                                                                                                                                              <  1:398461/251‑1 (MQ=255)                                                                                                                       gctgTAAATAAGCAAAGGCAGAGGCATAAAGTGCGGTCGGAAATTGCGCTGAATATTGCGTCGCCAGATGTTGCGTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAAc                                                                                                                                              >  1:250551/1‑231 (MQ=255)                                                                                                                       gctgTAAATAAGCAAAGGCAGAGGCATAAAGTGCGGTCGGAAATTGCGCTGAATATTGCGTCGCCAGATGTTGCGTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGtttt                                                                                                                          >  1:45204/1‑251 (MQ=255)                                                                                                                                 aGCAAAGGCAGAGGCATAAAGTGCGGTCGGAAATTGCGCTGAATATTGCGTCGCCAGATGTTGCGTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATcc                                                                                                                                                                                                                             >  1:386097/1‑142 (MQ=255)                                                                                                                                 aGCAAAGGCAGAGGCATAAAGTGCGGTCGGAAATTGCGCTGAATATTGCGTCGCCAGATGTTGCGTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGtt                                                                                                                >  1:625246/1‑251 (MQ=255)                                                                                                                                  gCAAAGGCAGAGGCATAAAGTGCGGTCGGAAATTGCGCTGAATATTGCGTCGCCAGATGTTGCGTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGtt                                                                                                                                                                >  1:263079/1‑202 (MQ=255)                                                                                                                                      aGGCAGAGGCATAAAGTGCGGTCGGAAATTGCGCTGAATATTGCGTCGCCAGATGTTGCGTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTgcg                                                                                                                                                          <  1:116781/204‑1 (MQ=255)                                                                                                                                      aGGCAGAGGCATAAAGTGCGGTCGGAAATTGCGCTGAATATTGCGTCGCCAGATGTTGCGTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGt                                                                                                                                          <  1:771165/220‑1 (MQ=255)                                                                                                                                       ggCAGAGGCATAAAGTGCGGTCGGAAATTGCGCTGAATATTGCGTCGCCAGATGTTGCGTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAg                                                                                                          >  1:876363/1‑251 (MQ=255)                                                                                                                                       ggCAGAGGCATAAAGTGCGGTCGGAAATTGCGCTGAATATTGCGTCGCCAGATGTTGCGTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAg                                                                                                          >  1:796287/1‑251 (MQ=255)                                                                                                                                         cAGAGGCATAAAGTGCGGTCGGAAATTGCGCTGAATATTGCGTCGCCAGATGTTGCGTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAGt                                                                                                         <  1:276839/250‑1 (MQ=255)                                                                                                                                           gagGCATAAAGTGCGGTCGGAAATTGCGCTGAATATTGCGTCGCCAGATGTTGCGTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAGTCAg                                                                                                      >  1:741870/1‑251 (MQ=255)                                                                                                                                            agGCATAAAGTGCGGTCGGAAATTGCGCTGAATATTGCGTCGCCAGATGTTGCGTATTTAACACCTCTTGCAGCAACATAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAGTc                                                                                                        <  1:447499/248‑1 (MQ=255)                                                                                                                                             ggCATAAAGTGCGGTCGGAAATTGCGCTGAATATTGCGTCGCCAGATGTTGCGTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACgg                                                                                                                                                                                            <  1:3506/163‑1 (MQ=255)                                                                                                                                             ggCATAAAGTGCGGTCGGAAATTGCGCTGAATATTGCGTCGCCAGATGTTGCGTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAGTCAg                                                                                                      >  1:821592/1‑249 (MQ=255)                                                                                                                                             ggCATAAAGTGCGGTCGGAAATTGCGCTGAATATTGCGTCGCCAGATGTTGCGTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAGTCAg                                                                                                      >  1:534686/1‑249 (MQ=255)                                                                                                                                                   aaGTGCGGTCGGAAATTGCGCTGAATATTGCGTCGCCAGATGTTGCGTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAGTCAGCTTGGAAg                                                                                              <  1:627591/251‑1 (MQ=255)                                                                                                                                                      tGCGGTCGGAAATTGCGCTGAATATTGCGTCGCCAGATGTTGCGTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAGTCAGCTTGGAAGc                                                                                             <  1:246609/249‑1 (MQ=255)                                                                                                                                                      tGCGGTCGGAAATTGCGCTGAATATTGCGTCGCCAGATGTTGCGTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAGTCAGCTTGGAAGCAc                                                                                           <  1:340216/251‑1 (MQ=255)                                                                                                                                                         ggTCGGAAATTGCGCTGAATATTGCGTCGCCAGATGTTGCGTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGATCGGTTGGTAAGTCAGCTTGGAAGCACCgg                                                                                        >  1:300365/1‑251 (MQ=255)                                                                                                                                                          gTCGGAAATTGCGCTGAATATTGCGTCGCCAGATGTTGCGTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTg                                                                                                                         <  1:767938/217‑1 (MQ=255)                                                                                                                                                            cGGAAATTGCGCTGAATATTGCGTCGCCAGATGTTGCGTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAg                                                                                                          <  1:526559/230‑1 (MQ=255)                                                                                                                                                              gAAATTGCGCTGAATATTGCGTCGCCAGATGTTGCGTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAAc                                                                                                                                              <  1:602516/192‑1 (MQ=255)                                                                                                                                                                 aTTGCGCTGAATATTGCGTCGCCAGATGTTGCGTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAGTCAGCTTGGAAGCACCGGAAAAATgg                                                                                <  1:24561/251‑1 (MQ=255)                                                                                                                                                                                     gCCAGATGTTGCGTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAGTCAGCTTGGAAGCACCGGAAAAATGGATTTTGACCGGCAAAAAAAt                                                            >  1:46794/1‑251 (MQ=255)                                                                                                                                                                                      ccAGATGTTGCGTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAGTCAGCTTGGAAGCACCGGAAAAATGGATTTTGACCGGCAAAAAAAt                                                            >  1:816347/1‑250 (MQ=255)                                                                                                                                                                                             ttGCGTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAGTCAGCTTGGAAGCACCGGAAAAATGGATTTTGACCGGCAAAAAAATCTCGATGc                                                    <  1:301692/251‑1 (MQ=255)                                                                                                                                                                                                 gTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAAc                                                                                                                                              <  1:303551/157‑1 (MQ=255)                                                                                                                                                                                                 gTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAg                                                                                                          >  1:782301/1‑193 (MQ=255)                                                                                                                                                                                                   aTTTAACACCTCTTGCAGCAATACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACAGGCACCCTGCCCTTTGTGCAAATTCCAAACGAGAAAACGCAATCTATTTTGTGTCATTTCTTGCGGTTTATGCGCCGCTTGGTAACAGGCTAAATCTTCGTGTTTTGCGAGTGGCTGGTAAGTCAGCGTGGAAGCACCGGAAAAATGGATTTTGACAGGCAAAAAAATCTCGATGCTGTaaa                                              >  1:612958/1‑251 (MQ=255)                                                                                                                                                                                                   aTTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATc                                                                                                                                    >  1:553823/1‑165 (MQ=255)                                                                                                                                                                                                   aTTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAGTCAGCTTGGAAGCACCGGAAAAATGGATTTTGACCGGCaaaaaa                                                              >  1:216611/1‑235 (MQ=255)                                                                                                                                                                                                   aTTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAGTCAGCTTGGAAGCACCGGAAAAATGGATTTTGACCGGCAAAAAAATCTCGATGCTGTaaa                                              >  1:799389/1‑251 (MQ=255)                                                                                                                                                                                                    tttAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAg                                                                                                          >  1:473167/1‑190 (MQ=255)                                                                                                                                                                                                       aaCACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAGTCAGCTTGGAAGCACCGGAAAAATGGATTTTGACCGGCAAAAAAATCTCGATGCTGTAAAAAAg                                          <  1:273205/251‑1 (MQ=255)                                                                                                                                                                                                        acacCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAGTCAGCTTGGAAGCACCGGAAAAATGGATTTTGACCGGCAAAAAAATCTCGATGCTGTAAAAAAGa                                         <  1:65345/251‑1 (MQ=255)                                                                                                                                                                                                           ccTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAGTCAGCTTGGAAGCACCGGAAAAATGGATTTTGACCGGCAAAAAAATCTCGATGCTGTAAAAAAGATAg                                      >  1:633749/1‑251 (MQ=255)                                                                                                                                                                                                           ccTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAGTCAGCTTGGAAGCACCGGAAAAATGGATTTTGACCGGCAAAAAAATCTCGATGCTGTAAAAAAGATAg                                      >  1:839214/1‑251 (MQ=255)                                                                                                                                                                                                           ccTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAGTCAGCTTGGAAGCACCGGAAAAATGGATTTTGACCGGCAAAAAAATCTCGATGCTGTAAAAAAGATAg                                      >  1:410295/1‑251 (MQ=255)                                                                                                                                                                                                           ccTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCAAGCGGTTGGTAAGTCAGCTTGGAAGCACCGGAAAAATGGATTTTGACCGGCAAAAAAATCTCGATGCTGTAAAAAAg                                          >  1:454474/1‑247 (MQ=255)                                                                                                                                                                                                            ctctTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAGTCAGCTTGGAAGCACCGGAAAAATGGATTTTGACCGGCAAAAAAATCTCGATGCTGTAAAAAAGATAgg                                     >  1:313866/1‑251 (MQ=255)                                                                                                                                                                                                             tctTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAg                                                                                                          <  1:77493/181‑1 (MQ=255)                                                                                                                                                                                                             tctTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAGTCAGCTTGGAAGCACCGGAAAAATGGATTTTGACCGGCAAAAAAATCTCGATGCTGTAAAAAAGATAgg                                     >  1:572316/1‑250 (MQ=255)                                                                                                                                                                                                             tctTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAGTCAGCTTGGAAGCACCGGAAAAATGGATTTTGACCGGCAAAAAAATCTCGATGCTGTAAAAAAGATAgg                                     >  1:551857/1‑250 (MQ=255)                                                                                                                                                                                                               ttGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAGTCAGCTTGGAAGCACCGGAAAAATGGATTTTGACCGGCAAAAAAATCTCGATGCTGTAAAAAAGATAGgcag                                  <  1:316969/251‑1 (MQ=255)                                                                                                                                                                                                                     caACACAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAGTCAGCTTGGAAGCACCGGAAAAATGGATTTTGACCGGCAAAAAAATCTCGATGCTGTAAAAAAGATAGGCAGCAAc                              <  1:863939/249‑1 (MQ=255)                                                                                                                                                                                                                       acacAAAGTCGCGCCCTTTGGCACAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCAttt                                                                                                                                                                         >  1:802275/1‑108 (MQ=255)                                                                                                                                                                                                                       acacAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATc                                                                                                                                    >  1:361523/1‑145 (MQ=255)                                                                                                                                                                                                                       acacAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAg                                                                                                          >  1:274600/1‑171 (MQ=255)                                                                                                                                                                                                                       acacAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAGTCAGCTTGGAAGCACCGGAAAAATGGATTTTGACCGGCAAAAAAATCTCGATGCTGTAAAAAAGATAGGCAGCAACGc                            >  1:588426/1‑249 (MQ=255)                                                                                                                                                                                                                       acacAAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAGTCAGCTTGGAAGCACCGGAAAAATGGATTTTGACCGGCAAAAAAATCTCGATGCTGTAAAAAAGATAGGCAGCAACGc                            >  1:410452/1‑249 (MQ=255)                                                                                                                                                                                                                        cacaAAGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAGTCAGCTTGGAAGCACCGGAAAAATGGATTTTGACCGGCAAAAAAATCTCGATGCTGTAAAAAAg                                          >  1:745219/1‑234 (MQ=255)                                                                                                                                                                                                                           aaaGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAGTCAGCTTGGAAGCACCGGAAAAATGGATTTTGACCGGCAAAAAAATCTCGATGCTGTAAAAAAGATAGGCAGCAACGCCTACTg                      <  1:183310/251‑1 (MQ=255)                                                                                                                                                                                                                            aaGTCGCGCCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAGTCAGCTTGGAAGCACCGGAAAAATGGATTTTGACCGGCAAAAAAATCTCGATGCTGTAAAAAAGATAGGCAGCAACGCCTACTg                      >  1:22260/1‑250 (MQ=255)                                                                                                                                                                                                                                  cgcCCTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAGTCAGCTTGGAAGCACCGGAAAAATGGATTTTGACCGGCAAAAAAATCTCGATGCTGTAAAAAAGATAGGCAGCAACGCCTACTGCGACTa                <  1:773257/250‑1 (MQ=255)                                                                                                                                                                                                                                     ccTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAGTCAGCTTGGAAGCACCGGAAAAATGGATTTTGACCGGCAAAAAAATCTCGATGCTGTAAAAAAGATAGGCAGCAACGCCTACTGCGACTATGcc            <  1:643090/251‑1 (MQ=255)                                                                                                                                                                                                                                      cTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAGTCAGCTTGGAAGCACCGGAAAAATgg                                                                                >  1:179854/1‑182 (MQ=255)                                                                                                                                                                                                                                      cTTTGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAGTCAGCTTGGAAGCACCGGAAAAATGGATTTTGACCGGCAAAAAAATCTCGATGCTGTAAAAAAGATAGGCAGCAACGCCTACTGCGACTATGCCa           >  1:860468/1‑251 (MQ=255)                                                                                                                                                                                                                                         tGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAGTCAGCTTGGAAGCACCGGAAAAATGGATTTTGACCGGCAAAAAAATCTCGATGCTGTAAAAAAGATAGGCAGCAACGCCTACTGCGACTATGCCAAAt        <  1:77503/251‑1 (MQ=255)                                                                                                                                                                                                                                         tGGCAAAACGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAGTCAGCTTGGAAGCACCGGAAAAATGGATTTTGACCGGCAAAAAAATCTCGATGCTGTAAAAAAGATAGGCAGCAACGCCTACTGCGACTATGCCAAAt        <  1:412766/251‑1 (MQ=255)                                                                                                                                                                                                                                               aaCGGAAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAGTCAGCTTGGAAGCACCGGAAAAATGGATTTTGACCGGCAAAAAAATCTCGATGCTGTAAAAAAGATAGGCAGCAACGCCTACTGCGACTATGCCAAATAAAGCg  >  1:213600/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      GCCAGAGGCAGACTCATGGCAGCACCGACTTTCGGAATGCGGATCCAGGGTTCCGGTTTTGAACCGGCGCAATAAAAGTGCGGTGGAAATTTTGAGAGAATTTCAACGCCCGATTGCTGCTGTAAATAAGCAAAGGCAGAGGCATAAAGTGCGGTCGGAAATTGCGCTGAATATTGCGTCGCCAGATGTTGCGTATTTAACACCTCTTGCAGCAACACAAAGTCGCGCCCTTTGGCAAAACGGGAAAGCGCCTGCTGCCAACCGGCATCCTGCCCTTTGTGCAAATTCCAAACGAGTAAACGGAATCTATTTTGTGTCATTTCTTGCGGTTTTTGCGCCGCTTGGTAACAGGTTAAATCTTCGTGTTTTGCGAGCGGTTGGTAAGTCAGCTTGGAAGCACCGGAAAAATGGATTTTGACCGGCAAAAAAATCTCGATGCTGTAAAAAAGATAGGCAGCAACGCCTACTGCGACTATGCCAAATAAAGCG  >  1/1582156‑1582644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑