Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,542,960	T→C	intergenic (‑26838/‑23104)	unknown ← / → unknown	tRNA‑Arg‑CCG/tRNA‑Asn‑GTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,542,960	0	T	C	100.0%	206.0 / NA	69	intergenic (‑26838/‑23104)	unknown/unknown	tRNA‑Arg‑CCG/tRNA‑Asn‑GTT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/40);  total (29/40)

GTTAATTAAGGCTTCAACCGCGCGGGCGGAAAAACCGCAAGACGGGAATTTCGGCGAACCTTTCATGTAAATAAGAATGGGGTTTTCGCTAATTTGTTTTTTGATTTTATCTAAAGTTTCCATAACATTCCTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCTTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAGTTTGGAATAAACGTGACTGCCACAATCACAACAACTGCGATTAAAATAAGCTATTTTTCATTTCTTATTATCAAATCTGACTACATAAAAAAAGACCTAAACTTTCGTTTAGGTCTTTATTATCTCTATTTGCTAACAATTAACAAATGAATTATTTAGTACCGTTAACAGCGATTT  >  1/1542714‑1543203                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    gTTAATTAAGGCTTCAACCGCGCGGGCGGAAAAACCGCAAGACGGGAATTTCGGCGAACCTTTCATGTAAATAAGAATGGGGTTTTCGCTAATTTGTTTTTTGATTTTATCTAAAGTTTCCATAACATTCCTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCAt                                                                                                                                                                                                                                                 >  1:533085/1‑251 (MQ=255)  ttaattaaGGCTTCAACCGCGCGGGCGGAAAAACCGCAAGACGGGAATTTCGGCGAACCTTTCATGTAAATAAGAATGGGGTTTTCGCTAATTTGTTTTTTGATTTTATCTAAAGTTTCCATAACATTCCTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCAt                                                                                                                                                                                                                                                 >  1:219687/1‑250 (MQ=255)    aattaaGGCTTCAACCGCGCGGGCGGAAAAACCGCAAGACGGGAATTTCGGCGAACCTTTCATGTAAATAAGAATGGGGTTTTCGCTAATTTGTTTTTTGATTTTATCTAAAGTTTCCATAACATTCCTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCa                                                                                                                                                                                                                                              >  1:770872/1‑251 (MQ=255)      ttaaGGCTTCAACCGCGCGGGCGGAAAAACCGCAAGACGGGAATTTCGGCGAACCTTTCATGTAAATAAGAATGGGGTTTTCGCTAATTTGTTTTTTGATTTTATCTAAAGTTTCCATAACATTCCTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCAtt                                                                                                                                                                                                                                            <  1:471782/251‑1 (MQ=255)                          gcggAAAAACCGCAAGACGGGAATTTCGGCGAACCTTTCATGTAAATAAGAATGGGGTTTTCGCTAATTTGTTTTTTGATTTTATCTAAAGTTTCTATAACATTCCTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTgggg                                                                                                                                                                                                                         <  1:886161/250‑1 (MQ=255)                          gcggAAAAACCGCAAGACGGGAATTTCGGCGAACCTTTCATGTAAATAAGAATGGGGTTTTCGCTAATTTGTTTTTTGATTTTATCTAAAGTTTCCATAACATTCCTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTgggg                                                                                                                                                                                                                         <  1:405428/250‑1 (MQ=255)                          gcggAAAAACCGCAAGACGGGAATTTCGGCGAACCTTTCATGTAAATAAGAATGGGGTTTTCGCTAATTTGTTTTTTGATTTTATCTAAAGTTTCCATAACATTCCTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTgggg                                                                                                                                                                                                                         <  1:350414/250‑1 (MQ=255)                           cggAAAAACCGCAAGACGGGAATTTCGGCGAACCTTTCATGTAAATAAGAATGGGGTTTTCGCTAATTTGTTTTTTGATTTTATCTAAAGTTTCCATAACATTCCTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGt                                                                                                                                                                                                                        <  1:482922/250‑1 (MQ=255)                               aaaaCCGCAAGACGGGAATTTCGGCGAACCTTTCATGTAAATAAGAATGGGGTTTTCGCTAATTTGTTTTTTGATTTTATCTAAAGTTTCCATAACATTCCTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGc                                                                                                                                                                                                                   <  1:859564/251‑1 (MQ=255)                                   ccGCAAGACGGGAATTTCGGCGAACCTTTCATGTAAATAAGAATGGGGTTTTCGCTAATTTGTTTTTTGATTTTATCTAAAGTTTCCATAACATTCCTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCttca                                                                                                                                                                                                               >  1:380012/1‑251 (MQ=255)                                      cAAGACGGGAATTTCGGCGAACCTTTCATGTAAATAAGAATGGGGTTTTCGCTAATTTGTTTTTTGATTTTATCTAAAGTTTCCATAACATTCCTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCttcattc                                                                                                                                                                                                            <  1:283296/251‑1 (MQ=255)                                                aTTTCGGCGAACCTTTCATGTAAATAAGAATGGGGTTTTCGCTAATTTGTTTTTTGATTTTATCTAAAGTTTCCATAACATTCCTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGAt                                                                                                                                                                                                  <  1:477102/251‑1 (MQ=255)                                                  ttCGGCGAACCTTTCATGTAAATAAGAATGGGGTTTTCGCTAATTTGTTTTTTGATTTTATCTAAAGTTTCCATAACATTCCTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGAttt                                                                                                                                                                                                <  1:72282/251‑1 (MQ=255)                                                       cGAACCTTTCATGTAAATAAGAATGGGGTTTTCGCTAATTTGTTTTTTGATTTTATCTAAAGTTTCCATAACATTCCTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGAc                                                                                                                                                                                            <  1:820988/250‑1 (MQ=255)                                                           ccTTTCATGTAAATAAGAATGGGGTTTTCGCTAATTTGTTTTTTGATTTTATCTAAAGTTTCCATAACATTCCTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGggc                                                                                                                                                                                                                                       >  1:873905/1‑203 (MQ=255)                                                           ccTTTCATGTAAATAAGAATGGGGTTTTCGCTAATTTGTTTTTTGATTTTATCTAAAGTTTCCATAACATTCCTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGt                                                                                                                                                                                                                        <  1:643346/218‑1 (MQ=255)                                                           ccTTTCATGTAAATAAGAATGGGGTTTTCGCTAATTTGTTTTTTGATTTTATCTAAAGTTTCCATAACATTCCTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGata                                                                                                                                                                                       >  1:674776/1‑251 (MQ=255)                                                           ccTTTCATGTAAATAAGAATGGGGTTTTCGCTAATTTGTTTTTTGATTTTATCTAAAGTTTCCATAACATTCCTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGata                                                                                                                                                                                       >  1:200692/1‑251 (MQ=255)                                                                cATGTAAATAAGAATGGGGTTTTCGCTAATTTGTTTTTTGATTTTATCTAAAGTTTCCATAACATTCCTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAGt                                                                                                                                                                                  <  1:847585/251‑1 (MQ=255)                                                                           gAATGGGGTTTTCGCTAATTTGTTTTTTGATTTTATCTAAAGTTTCCATAACATTCCTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAGTTTgg                                                                                                                                                                              <  1:841931/244‑1 (MQ=255)                                                                            aaTGGGGTTTTCGCTAATTTGTTTTTTGATTTTATCTAAAGTTTCCATAACATTCCTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAGTTTGGAATAAACg                                                                                                                                                                      <  1:564791/251‑1 (MQ=255)                                                                                   ttttCGCTAATTTGTTTTTTGATTTTATCTAAAGTTTCCATAACATTCCTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAGTTTGGAATAAACGTGACTGc                                                                                                                                                               <  1:308114/251‑1 (MQ=255)                                                                                               tGTTTTTTGATTTTATCTAAAGTTTCCATAACATTCCTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAGTTTGGAATAAACGTGACTGCCACAATCacaac                                                                                                                                                   <  1:157964/251‑1 (MQ=255)                                                                                                  tttttGATTTTATCTAAAGTTTCCATAACATTCCTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAGTTTGGAATAAACGTGACTGCCACAATCACaacaac                                                                                                                                                <  1:224808/251‑1 (MQ=255)                                                                                                  tttttGATTTTATCTAAAGTTTCCATAACATTCCTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAGTTTGGAATAAACGTGACTGCCACAATCACaacaac                                                                                                                                                <  1:137815/251‑1 (MQ=255)                                                                                                    tttGATTTTATCTAAAGTTTCCATAACATTCCTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAGTTTGGAATAAACGTGACTGCCACAATCACAACAACTg                                                                                                                                              <  1:637057/251‑1 (MQ=255)                                                                                                        aTTTTATCTAAAGTTTCCATAACATTCCTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGggc                                                                                                                                                                                                                                       <  1:602031/158‑1 (MQ=255)                                                                                                            tATCTAAAGTTTCCATAACATTCCTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATc                                                                                                                                                                                                                                                <  1:397729/145‑1 (MQ=255)                                                                                                            tATCTAAAGTTTCCATAACATTCCTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTgggg                                                                                                                                                                                                                         <  1:782240/168‑1 (MQ=255)                                                                                                              tCTAAAGTTTCCATAACATTCCTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAGTTTGGAATAAACGTGACTGCCACAATCACAACAACTGCGATTAAAAt                                                                                                                                    <  1:811056/251‑1 (MQ=255)                                                                                                                      ttCCATAACATTCCTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAGTTTGGAATAAACGTGACTGCCACAATCACAACAACTGCGATTAAAATAAGCTAtt                                                                                                                            >  1:120681/1‑251 (MQ=255)                                                                                                                      ttCCATAACATTCCTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATAACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGc                                                                                                                                                                                                                   >  1:372888/1‑164 (MQ=255)                                                                                                                        ccATAACATTCCTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAg                                                                                                                                                                                   <  1:743892/194‑1 (MQ=255)                                                                                                                        ccATAACATTCCTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAGTTTGGAATAAACGTGACTGCCACAATCACAACAACTGCGATTAAAATAAGc                                                                                                                                >  1:499860/1‑245 (MQ=255)                                                                                                                          aTAACATTCCTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTgggg                                                                                                                                                                                                                         >  1:689885/1‑154 (MQ=255)                                                                                                                          aTAACATTCCTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAGTTTGGAATAAACGTGACTGCCACAATCACAACAACTGCGATTAAAATAAGCTAttttt                                                                                                                         >  1:106585/1‑250 (MQ=255)                                                                                                                            aaCATTCCTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAGTTTGGAATAAACGTGACTGCCACAATCACAACAACTGCGATTAAAATAAGCTATTTTTCAt                                                                                                                      <  1:622078/251‑1 (MQ=255)                                                                                                                              cATTCCTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGggc                                                                                                                                                                                                                                       >  1:816960/1‑136 (MQ=255)                                                                                                                              cATTCCTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAGTTTGGAATAAACGTGACTGCCACAATCACAACAACTGCGATTAAAATAAGCTATTTTTCAttt                                                                                                                    >  1:129777/1‑251 (MQ=255)                                                                                                                                ttCCTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCCCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAGTTTGGAATAAACGTGACTGCCACAATCACAACAACTGCGATTAAAATAAGCTAttt                                                                                                                           >  1:841439/1‑242 (MQ=255)                                                                                                                                 tCCTTGTGTTCATTGAAGGAAAAAAACGCGCGCATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAGTTTGGAATAAACGTGACTGCCACAATCACAACAACTGCGATTAAAATAAGCTATTTTTCATTTCtt                                                                                                                 >  1:737633/1‑251 (MQ=255)                                                                                                                                  ccTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAGTTTGGAATAAACGAGACTGCCACAATCACAACAACTg                                                                                                                                              >  1:40476/1‑221 (MQ=255)                                                                                                                                   cTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAGt                                                                                                                                                                                  >  1:524775/1‑184 (MQ=255)                                                                                                                                      gtgtTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAg                                                                                                                                                                                                                                          >  1:468710/1‑125 (MQ=255)                                                                                                                                                   gAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGAc                                                                                                                                                                                            <  1:521368/158‑1 (MQ=255)                                                                                                                                                   gAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAGTTTGGAATAAACGTGACTGCCACAATCACAACAACTGCGATTAAAATAAGCTATTTTTCATTTCTTATTATCAAATCTGACt                                                                                                 <  1:125846/249‑1 (MQ=255)                                                                                                                                                             cgcgTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGAt                                                                                                                                                                                                  >  1:201197/1‑142 (MQ=255)                                                                                                                                                             cgcgTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAGTTTGGAATAAACGTGACTGCCACAATCACAACAACTGCGATTAAAATAAGCTATTTTTCATTTCTTATTATCAAATc                                                                                                      <  1:139871/234‑1 (MQ=255)                                                                                                                                                                 tATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTg                                                                                                                                                                                          <  1:36365/146‑1 (MQ=255)                                                                                                                                                                   ttGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAGTTTGGAATAAACGTGACTGCCACAATCacaaca                                                                                                                                                  <  1:297302/184‑1 (MQ=255)                                                                                                                                                                   ttGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAGTTTGGAATAAACGTGACTGCCACAATCACAACAACTg                                                                                                                                              >  1:21856/1‑188 (MQ=255)                                                                                                                                                                     gTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAGTTTGGAATAAACGTGACTGCCACAATCACAACAACTGCGATTAAAATAAGCTATTTTTCATTTCTTATTATCAAATCTGACTACATAAAAAAAg                                                                                     <  1:583976/243‑1 (MQ=255)                                                                                                                                                                       aTCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAGTTTGGAATAAACGTGACTGCCACAATCACaacaac                                                                                                                                                <  1:7700/182‑1 (MQ=255)                                                                                                                                                                                       cccACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAGTTTGGAATAAACGTGACTGCCACAATCACAACAACTGCGATTAAAATAAGCTATTTTTCATTTCTTATTATCAAATCTGACTACATAAAAAAAGACCTAAACTTTCGTTTAGGTCTttat                                                           <  1:855385/251‑1 (MQ=255)                                                                                                                                                                                         caccaATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAGtt                                                                                                                                                                                 >  1:685489/1‑131 (MQ=255)                                                                                                                                                                                           ccaATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAg                                                                                                                                                                                   >  1:608781/1‑127 (MQ=255)                                                                                                                                                                                           ccaATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAGTTTGGAATAAACg                                                                                                                                                                      <  1:555856/140‑1 (MQ=255)                                                                                                                                                                                            caATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCCTTCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAGTTTGGAATAAACGTGACTGCCACAATCACAACAACTGCGATTAAAATAAGCTCTTTTTCATTTCTTATTATCAAATCTGACTAc                                                                                               >  1:182376/1‑210 (MQ=255)                                                                                                                                                                                            caATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAGTTTGGAATAAACGTGACTGCCACAATCACAACAACTGCGATTAAAATAAGCTATTTTTCAt                                                                                                                      <  1:361753/187‑1 (MQ=255)                                                                                                                                                                                            caATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAGTTTGGAATAAACGTGACTGCCACAATCACAACAACTGCGATTAAAATAAGCTATTTTTCATTTCTTATTATCAAATCTGACt                                                                                                 >  1:652934/1‑208 (MQ=255)                                                                                                                                                                                              aTAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAGTTTGGAATAAACGTGACTGCCACAATCACAACAACTGCGATTAAAATAAGCTATTTTTCATTTCTTATTATCAAATCTGACTACATAAAAAAAGACCTAAACTTTCGTTTAGGTCTTTATTAtct                                                      >  1:16477/1‑249 (MQ=255)                                                                                                                                                                                                   ggTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAGTTTGGAATAAACGTGACTGCCACAATCACAACAACTGCGATTAAAATAAGCTATTTTTCATTTc                                                                                                                   <  1:568316/183‑1 (MQ=255)                                                                                                                                                                                                                      ggTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAGTTTGGAATAAACGTGACTGCCACAATCACAACAACTGCGATTAAAATAAGCTATTTTTCATTTCTTATTATCAAATCTGACTACATAAAAAAAGACCTAAACTTTCGTTTAGGTCTTTATTATCTCTATTTGCTAACAATTAACAAATGaa                            >  1:51451/1‑251 (MQ=255)                                                                                                                                                                                                                       gTATTTACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAGTTTGGAATAAACGTGACTGCCACAATCACAACAACTGCGATTAAAATAAGCTATTTTTCATTTCTTATTATCAAATCTGACTACATAAAAAAAGACCTAAACTTTCGTTTAGGTCTTTATTAtct                                                      <  1:401488/224‑1 (MQ=255)                                                                                                                                                                                                                            tACCGGCTACTACCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAGTTTGGAATAAACGTGACTGCCACAATCACAACAACTGCGATTAAAATAAGCTATTTTTCATTTCTTATTATCAAATCTGACTACATAAAAAAAGACCTAAACTTTCGTTTAGGTCTTTATTATCTCTATTTGCTAACAATTAACAAATGAATTAttt                      <  1:314519/251‑1 (MQ=255)                                                                                                                                                                                                                                       acCAAAACGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAGTTTGGAATAAACGTGACTGCCACAATCACaacaac                                                                                                                                                <  1:318457/118‑1 (MQ=255)                                                                                                                                                                                                                                          aaaaCGCCCTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAGTTTGGAATAAACGTGACTGCCACAATCACAACAACTGCGATTAAAATAAGCTATTTTTCATTTCTTATTATCAAATCTGACTACATAAAAAAAGACCTAAACTTTCGTTTAGGTCTTTATTATCTCTATTTGCTAACAATTAACAAATGAATTATTTAGTACCGTTAACAg        <  1:680486/251‑1 (MQ=255)                                                                                                                                                                                                                                                cccTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAGTTTGGAATAAACGTGACTGCCACAATCACAACAACTGCGATTAAAATAAGCTATTTTTCATTTCTTATTATCAAATCTGACTACATAAAAAAAGACCTAAACTTTCGTTTAGGTCTTTATTATCTCTATTTGCTAACAATTAACAAATGAATTATTTAGTACCGTTAACAGCGAttt  >  1:641820/1‑251 (MQ=255)                                                                                                                                                                                                                                                cccTCGCCTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAGTTTGGAATAAACGTGACTGCCACAATCACAACAACTGCGATTAAAATAAGCTATTTTTCATTTCTTATTATCAAATCTGACTACATAAAAAAAGACCTAAACTTTCGTTTAGGTCTTTATTATCTCTATTTGCTAACAATTAACAAATGAATTATTTAGTACCGTTAACAGCGAttt  >  1:475282/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    GTTAATTAAGGCTTCAACCGCGCGGGCGGAAAAACCGCAAGACGGGAATTTCGGCGAACCTTTCATGTAAATAAGAATGGGGTTTTCGCTAATTTGTTTTTTGATTTTATCTAAAGTTTCCATAACATTCCTTGTGTTCATTGAAGGAAAAAAACGCGCGTATTGTATCACAAACAATTTTCCCCACCAATAAAGGTCTGAATGTTTGGCTCGGGTATTTACCGGCTACTACCAAAACGCCCTCGCTTCATCCATTAGGGCGGCAAGGTTTGGGGTAATGCTTCATTCAGCACATGATTTTGACTGATATAAGTTTGGAATAAACGTGACTGCCACAATCACAACAACTGCGATTAAAATAAGCTATTTTTCATTTCTTATTATCAAATCTGACTACATAAAAAAAGACCTAAACTTTCGTTTAGGTCTTTATTATCTCTATTTGCTAACAATTAACAAATGAATTATTTAGTACCGTTAACAGCGATTT  >  1/1542714‑1543203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑