Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,541,716	C→T	intergenic (‑25594/‑24348)	unknown ← / → unknown	tRNA‑Arg‑CCG/tRNA‑Asn‑GTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,541,716	0	C	T	100.0%	216.9 / NA	77	intergenic (‑25594/‑24348)	unknown/unknown	tRNA‑Arg‑CCG/tRNA‑Asn‑GTT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/41);  total (36/41)

TCATTCAGTTGTGTGAAAAGGGATTGTTGTAAAGGATTAAGTTCTTGTTTTATCGTTCTTTTATTCATTCTGATTTACTTTTGAAAGCGCCGTAAGCTAAACCAAGCCAGCCGATTAAAAATAGCGTGCCGCCGATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGCGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGATATTTCACGCCGGTGTCAATCCACGCTAAGGCTTTCGCGTCCAACACTTGGCTTAAACCGTGCGCCGCAAACGCACCGATAGTCACACATAAAAAACCGCTCACGGCGGCAATCAATAACCATTTGTTTTTCATTTAAAATTTTCCTATTAATAATGCCAAAA  >  1/1541476‑1541958                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                   tCATTCAGTTGTGTGAAAAGGGATTGTTGTAAAGGATTAAGTTCTTGTTTTATCGTTCTTTTATTCATTCTGATTTACTTTTGAAAGCGCCGTAAGCTAAACCAAGCCAGCCGATTAAAAATAGCGTGCCGCCGATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCGAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTttgttg                                                                                                                                                                                                                                          >  1:434008/1‑251 (MQ=255) tCATTCAGTTGTGTGAAAAGGGATTGTTGTAAAGGATTAAGTTCTTGTTTTATCGTTCTTTTATTCATTCTGATTTACTTTTGAAAGCGCCGTAAGCTAAACCAAGCCAGCCGATTAAAAATAGCGTGCCGCCGATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTttgttg                                                                                                                                                                                                                                          >  1:96981/1‑251 (MQ=255) tCATTCAGTTGTGTGAAAAGGGATTGTTGTAAAGGATTAAGTTCTTGTTTTATCGTTCTTTTATTCATTCTGATTTACTTTTGAAAGCGCCGTAAGCTAAACCAAGCCAGCCGATTAAAAATAGCGTGCCGCCGATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTttgttg                                                                                                                                                                                                                                          >  1:33108/1‑251 (MQ=255)        gTTGTGTGAAAAGGGATTGTTGTAAAGGATTAAGTTCTTGTTTTATCGTTCTTTTATTCATTCTGATTTACTTTTGAAAGCGCCGTAAGCTAAACCAAGCCAGCCGATTAAAAATAGCGTGCCGCCGATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCaa                                                                                                                                                                                                                                   >  1:467292/1‑251 (MQ=255)        gTTGTGTGAAAAGGGATTGTTGTAAAGGATTAAGTTCTTGTTTTATCGTTCTTTTATTCATTCTGATTTACTTTTGAAAGCGCCGTAAGCTAAACCAAGCCAGCCGATTAAAAATAGCGTGCCGCCGATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCaa                                                                                                                                                                                                                                   >  1:357340/1‑251 (MQ=255)           gtgtgAAAAGGGATTGTTGTAAAGGATTAAGTTCTTGTTTTATCGTTCTTTTATTCATTCTGATTTACTTTTGAAAGCGCCGTAAGCTAAACCAAGCCAGCCGATTAAAAATAGCGTGCCGCCGATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATg                                                                                                                                                                                                                                >  1:750217/1‑251 (MQ=255)                     ggATTGTTGTAAAGGATTAAGTTCTTGTTTTATCGTTCTTTTATTCATTCTGATTTACTTTTGAAAGCGCCGTAAGCTAAACCAAGCCAGCCGATTAAAAATAGCGTGCCGCCGATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACa                                                                                                                                                                                                                      >  1:188863/1‑251 (MQ=255)                         tgttgtAAAGGATTAAGTTCTTGTTTTATCGTTCTTTTATTCATTCTGATTTACTTTTGAAAGCGCCGTAAGCTAAACCAAGCCAGCCGATTAAAAATAGCGTGCCGCCGATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAAt                                                                                                                                                                                                                  <  1:327566/251‑1 (MQ=255)                           ttgtAAAGGATTAAGTTCTTGTTTTATCGTTCTTTTATTCATTCTGATTTACTTTTGAAAGCGCCGTAAGCTAAACCAAGCCAGCCGATTAAAAATAGCGTGCCGCCGATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATtg                                                                                                                                                                                                                <  1:794519/251‑1 (MQ=255)                           ttgtAAAGGATTAAGTTCTTGTTTTATCGTTCTTTTATTCATTCTGATTTACTTTTGAAAGCGCCGTAAGCTAAACCAAGCCAGCCGATTAAAAATAGCGTGCCGCCGATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATtg                                                                                                                                                                                                                <  1:816063/251‑1 (MQ=255)                              tAAAGGATTAAGTTCTTGTTTTATCGTTCTTTATTTCATTCTGATTTACTTTTGAAAGCGCCGTAAGCTAAACCAAGCCAGTCGATTAAAAATAGCGTGCCGCCGATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGc                                                                                                                                                                                                             <  1:449310/251‑1 (MQ=255)                                  ggATTAAGTTCTTGTTTTATCGTTCTTTTATTCATTCTGATTTACTTTTGAAAGCGCCGTAAGCTAAACCAAGCCAGCCGATTAAAAATAGCGTGCCGCCGATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAAt                                                                                                                                                                                                                  <  1:497964/242‑1 (MQ=255)                                  ggATTAAGTTCTTGTTTTATCGTTCTTTTATTCATTCTGATTTACTTTTGAAAGCGCCGTAAGCTAAACCAAGCCAGCCGATTAAAAATAGCGTGCCGCCGATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATg                                                                                                                                                                                                         >  1:131097/1‑251 (MQ=255)                                  ggATTAAGTTCTTGTTTTATCGTTCTTTTATTCATTCTGATTTACTTTTGAAAGCGCCGTAAGCTAAACCAAGCCAGCCGATTAAAAATAGCGTGCCGCCGATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATg                                                                                                                                                                                                         >  1:788731/1‑251 (MQ=255)                                  ggATTAAGTTCTTGTTTTATCGTTCTTTTATTCATTCTGATTTACTTTTGAAAGCGCCGTAAGCTAAACCAAGCCAGCCGATTAAAAATAGCGTGCCGCCGATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATg                                                                                                                                                                                                         >  1:205253/1‑251 (MQ=255)                                  ggATTAAGTTCTTGTTTTATCGTTCTTTTATTCATTCTGATTTACTTTTGAAAGCGCCGTAAGCTAAACCAAGCCAGCCGATTAAAAATAGCGTGCCGCCGATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATg                                                                                                                                                                                                         >  1:323151/1‑251 (MQ=255)                                    aTTAAGTTCTTGTTTTATCGTTCTTTTATTCATTCTGATTTACTTTTGAAAGCGCCGTAAGCTAAACCAAGCCAGCCGATGAAAAATAGCGTGCCGCCGATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGc                                                                                                                                                                                                        <  1:425324/250‑1 (MQ=255)                                              tGTTTTATCGTTCTTTTATTCATTCTGATTTACTTTTGAAAGCGCCGTAAGCTAAACCAAGCCAGCCGATTAAAAATAGCGTGCCGCCGATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCatg                                                                                                                                                                                             <  1:606166/251‑1 (MQ=255)                                                ttttATCGTTCTTTTATTCATTCTGATTTACTTTTGAAAGCGCCGTAAGCTAAACCAAGCCAGCCGATTAAAAATAGCGTGCCGCCGATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCatga                                                                                                                                                                                            <  1:551332/250‑1 (MQ=255)                                                  ttATCGTTCTTTTATTCATTCTGATTTACTTTTGAAAGCGCCGTAAGCTAAACCAAGCCAGCCGATTAAAAATAGCGTGCCGCCGATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATgg                                                                                                                                                                                         <  1:893208/251‑1 (MQ=255)                                                          cTTTTATTCATTCTGATTTACTTTTGAAAGCGCCGTAAGCTAAACCAAGCCAGCCGATTAAAAATAGCGTGCCGCCGATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGa                                                                                                                                                                                                           >  1:344096/1‑225 (MQ=255)                                                          cTTTTATTCATTCTGATTTACTTTTGAAAGCGCCGTAAGCTAAACCAAGCCAGCCGATTAAAAATAGCGTGCCGCCGATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAggg                                                                                                                                                                                   >  1:853706/1‑249 (MQ=255)                                                                ttcattcTGATTTACTTTTGAAAGCGCCGTAAGCTAAACCAAGCCAGCCGATTAAAAATAGCGTGCCGCCGATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAAc                                                                                                                                                                           <  1:346714/251‑1 (MQ=255)                                                                ttcattcTGATTTACTTTTGAAAGCGCCGTAAGCTAAACCAAGCCAGCCGATTAAAAATAGCGTGCCGCCGATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAAc                                                                                                                                                                           <  1:359048/251‑1 (MQ=255)                                                                ttcattcTGATTTACTTTTGAAAGCGCCGTAAGCTAAACCAAGCCAGCCGATTAAAAATAGCGTGCCGCCGATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAAc                                                                                                                                                                           <  1:279321/251‑1 (MQ=255)                                                                   attcTGATTTACTTTTGAAAGCGCCGTAAGCTAAACCAAGCCAGCCGATTAAAAATAGCGTGCCGCCGATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACAtt                                                                                                                                                                        >  1:340417/1‑251 (MQ=255)                                                                    ttcTGATTTACTTTTGAAAGCGCCGTAAGCTAAACCAAGCCAGCCGATTAAAAATAGCGTGCCGCCGATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATtg                                                                                                                                                                                                                >  1:320786/1‑210 (MQ=255)                                                                      cTGATTTACTTTTGAAAGCGCCGTAAGCTAAACCAAGCCAGCCGATTAAAAATAGCGTGCCGCCGATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGa                                                                                                                                                                     >  1:462580/1‑251 (MQ=255)                                                                        gATTTACTTTTGAAAGCGCCGTAAGCTAAACCAAGCCAGCCGATTAAAAATAGCGTGCCGCCGATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGata                                                                                                                                                                   >  1:629415/1‑251 (MQ=255)                                                                             aCTTTTGAAAGCGCCGTAAGCTAAACCAAGCCAGCCGATTAAAAATAGCGTGCCGCCGATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTc                                                                                                                                                                                                                            <  1:290155/189‑1 (MQ=255)                                                                              cTTTTGAAAGCGCCGTAAGCTAAACCAAGCCAGCCGATTAAAAATAGCGTGCCGCCGATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAAc                                                                                                                                                                           >  1:133701/1‑237 (MQ=255)                                                                                              aaGCTAAACCAAGCCAGCCGATTAAAAATAGCGTGCCGCCGATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCatgat                                                                                                                                                                                           >  1:449645/1‑205 (MQ=255)                                                                                               aGCTAAACCAAGCCAGCCGATTAAAAATAGCGTGCCGCCGATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGc                                                                                                                                                                                                             <  1:841233/186‑1 (MQ=255)                                                                                                 cTAAACCAAGCCAGCCGATTAAAAATAGCGTGCCGCCGATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGATATTTCACGCCGGTGTCAATCCACGCt                                                                                                                                          <  1:734496/251‑1 (MQ=255)                                                                                                   aaaCCAAGCCAGCCGATTAAAAATAGCGTGCCGCCGATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATgg                                                                                                                                                                                         <  1:151476/202‑1 (MQ=255)                                                                                                    aaCCAAGCCAGCCGATTAAAAATAGCGTGCCGCCGATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGATATTTCACGCCGGTGTCAATCCACGCTAAg                                                                                                                                       >  1:23852/1‑251 (MQ=255)                                                                                                    aaCCAAGCCAGCCGATTAAAAATAGCGTGCCGCCGATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGATATTTCACGCCGGTGTCAATCCACGCTAAg                                                                                                                                       >  1:49768/1‑251 (MQ=255)                                                                                                                    tAAAAATAGCGTGCCGCCGATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATgg                                                                                                                                                                                                                               <  1:640729/147‑1 (MQ=255)                                                                                                                       aaaTAGCGTGCCGCCGATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGATATTTCACGCCGGTGTCAATCCACGCTAAGGc                                                                                                                                     <  1:66750/234‑1 (MQ=255)                                                                                                                          tAGCGTGCCGCCGATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGATATTTCACGCCGGTGTCAATCCACGCTAAGGCTTTCGCGTCCAACACTTGGc                                                                                                                 <  1:704398/251‑1 (MQ=255)                                                                                                                                 ccgccgATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGATATTTCACGCCGGTGTCAATCCACGCTAAGGCTTTCGCGTCCAACACTTGGCTTAAAc                                                                                                           <  1:877687/250‑1 (MQ=255)                                                                                                                                 ccgccgATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGATATTTCACGCCGGTGTCAATCCACGCTAAGGCTTTCGCGTCCAACACTTGGCTTAAAc                                                                                                           <  1:521075/250‑1 (MQ=255)                                                                                                                                   gccgATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAAt                                                                                                                                                                                                                  <  1:116533/145‑1 (MQ=255)                                                                                                                                          ggCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGATATTTCACGCCGGTGTCAATCCACGCTAAGGCTTTCGCGTCCAACACTTGGCTTAAACCg                                                                                                         <  1:130878/243‑1 (MQ=255)                                                                                                                                           gCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGATATTTCACGCCGGTGTCAATc                                                                                                                                                <  1:888841/203‑1 (MQ=255)                                                                                                                                                aGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGATATTTCACGCCGGTGTCAATCCACGCTAAGGCTTTCGCGTCCAACACTTGGCTTAAACCGTGCGCCGCAAACg                                                                                            >  1:558085/1‑250 (MQ=255)                                                                                                                                                       ccATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGATATTTCACGCCGGTGTCAATCCACGCTAAGGCTTTCGCGTCCAACACTTGGCTTAAACCGTGCGCCGCAAACGCACCGATa                                                                                    >  1:355445/1‑251 (MQ=255)                                                                                                                                                        cATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGATATTTCACGCCGGTGTCAATCCACGCTAAGGCTTTCGCGTCCAACACTTGGCTTAAACCGTGCGCCGCAAACGCACCGATAg                                                                                   <  1:47874/251‑1 (MQ=255)                                                                                                                                                                    tGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGATATTTCACGCCGGTGTCAATCCACGCTAAGGCTTTCGCGTCCAACACTTGGCTTAAACCGTGCGCCGCAAACGCACCGATAGTCACACATaaaa                                                                       <  1:730777/251‑1 (MQ=255)                                                                                                                                                                      aGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCCACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGATATTTCACGCCGGTGTCAATCCACGCTAAGGCTTTCGCGTCCAACACTTGGCTTAAACCGTGCGCCGCAAACGCACCGATAGTCACACATaaaaaa                                                                     >  1:859495/1‑251 (MQ=255)                                                                                                                                                                      aGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGATATTTCACGCCGGTGTCAATCCACGCTAAGGCTTTCGCGTCCAACACTTGGCTTAAACCGTGCGCCGCAAACGCACCGATAGTCACACATaaaaaa                                                                     >  1:250717/1‑251 (MQ=255)                                                                                                                                                                      aGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGATATTTCACGCCGGTGTCAATCCACGCTAAGGCTTTCGCGTCCAACACTTGGCTTAAACCGTGCGCCGCAAACGCACCGATAGTCACACATaaaaaa                                                                     >  1:300786/1‑251 (MQ=255)                                                                                                                                                                       gCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGATATTTCACGCCGGTGTCAATc                                                                                                                                                <  1:508735/175‑1 (MQ=255)                                                                                                                                                                        ccAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGATATTTCACGCCGGTGTCAATCCACGCTAAGGCTTTCGCGTCCAACACTTGGCTTAAACCGTGCGCCGCAAACGCACCGATAGTCACACATAAAAAAc                                                                    <  1:733482/250‑1 (MQ=255)                                                                                                                                                                        ccAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGATATTTCACGCCGGTGTCAATCCACGCTAAGGCTTTCGCGTCCAACACTTGGCTTAAACCGTGCGCCGCAAACGCACCGATAGTCACACATAAAAAAc                                                                    <  1:154715/250‑1 (MQ=255)                                                                                                                                                                        ccAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGATATTTCACGCCGGTGTCAATCCACGCTAAGGCTTTCGCGTCCAACACTTGGCTTAAACCGTGCGCCGCAAACGCACCGATAGTCACACATAAAAAAc                                                                    <  1:553589/250‑1 (MQ=255)                                                                                                                                                                        ccAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGATATTTCACGCCGGTGTCAATCCACGCTAAGGCTTTCGCGTCCAACACTTGGCTTAAACCGTGCGCCGCAAACGCACCGATAGTCACACATAAAAAAc                                                                    <  1:39486/250‑1 (MQ=255)                                                                                                                                                                            gTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGATATTTCACGCCGGTGTCAATCCACGCTAAGGCTTTCGCGTCCAACACTTGGCTTAAACCGTGCGCCGCAAACGCACCGATAg                                                                                   >  1:689844/1‑231 (MQ=255)                                                                                                                                                                            gTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGATATTTCACGCCGGTGTCAATCCACGCTAAGGCTTTCGCGTCCAACACTTGGCTTAAACCGTGCGCCGCAAACGCACCGATAGTCACACATaaaa                                                                       >  1:836491/1‑243 (MQ=255)                                                                                                                                                                                   ggCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGATATTTCACGCCGGTGTCAATCCACGCTAAGGCTTTCGCGTCCAACACTTGGCTTAAAc                                                                                                           >  1:321074/1‑200 (MQ=255)                                                                                                                                                                                   ggCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGATATTTCACGCCGGTGTCAATCCACGCTAAGGCTTTCGCGTCCAACACTTGGCTTAAACCGTGCGCCGCAAACGCACCGATAGTCACACATAAAAAACCGCTCAcggcgg                                                        >  1:506420/1‑251 (MQ=255)                                                                                                                                                                                     cGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGATATTTCACGCCGGTGTCAATCCACGCTAAGGCTTTCGCGTCCAACACTTGGCTTAAACCGTGCGCCGCAAACGCACCGATAGt                                                                                  <  1:345069/223‑1 (MQ=255)                                                                                                                                                                                     cGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGATATTTCACGCCGGTGTCAATCCACGCTAAGGCTTTCGCGTCCAACACTTGGCTTAAACCGTGCGCCGCAAACGCACCGATAGTCACACATAAAAAACCGCTCACGGCGGca                                                      >  1:392105/1‑251 (MQ=255)                                                                                                                                                                                              tGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGATATTTCACGCCGGTGTCAATCCACGCTAAGGCTTTCGCGTCCAACACTTGGCTTAAACCGTGCGCCGCAAACGCACCGATAGTCACACATAAAAAACCGCTCACGGCGGCAATCAATAAc                                             <  1:87404/251‑1 (MQ=255)                                                                                                                                                                                                ccGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGATATTTCACGCCGGTGTCAATCCACGCTAAGGCTTTCGCGTCCAACACTTGGCTTAAACCGTGCGCCGCAAACGCACCGATAGTCACACATAAAAAACCGCTCACGGCGGCAATCAATAACCa                                           <  1:646637/251‑1 (MQ=255)                                                                                                                                                                                                ccGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGATATTTCACGCCGGTGTCAATCCACGCTAAGGCTTTCGCGTCCAACACTTGGCTTAAACCGTGCGCCGCAAACGCACCGATAGTCACACAT‑AAAAACCGCTCACGGCGGCAATCAATAACCa                                           <  1:404758/250‑1 (MQ=255)                                                                                                                                                                                                   cTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGaa                                                                                                                                                                            <  1:732548/119‑1 (MQ=255)                                                                                                                                                                                                          gaAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGATATTTCACGCCGGTGTCAATCCACGCTAAGGCTTTCGCGTCCAACACTTGGCTTAAAc                                                                                                           >  1:494640/1‑177 (MQ=255)                                                                                                                                                                                                              gATTCCGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGATATTTCACGCCGGTGTCAATCCACGCTAAGGCTTTCGCGTCCAACACTTGGCTTAAACCGTGCGCCGCAAACGCACCGATAGTCACACATAAAAAACCGCTCACGGCGGCAATCAATAACCATTTGTTTTTCAttt                             >  1:576359/1‑251 (MQ=255)                                                                                                                                                                                                                   cGAATGACCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGATATTTCACGCCGGTGTCAATCCACGCTAAGGCTTTCGCGTCCAACACTTGGCTTAAACCGTGCGCCGCAAACGCACCGATAGTCACACATAAAAAACCGCTCACGGCGGCAATCAATAACCATTTGTTTTTCATTTAAAAt                        <  1:20348/251‑1 (MQ=255)                                                                                                                                                                                                                         aCCACGCGCCGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGATATTTCACGCCGGTGTCAATCCACGCTAAGGCTTTCGCGTCCAACACTTGGCTTAAACCGTGCGCCGCAAACGCACCGATAGTCACACATAAAAAACCGCTCACGGCGGCAATCAATAACCATTTGTTTTTCATTTAAAATTTTc                    >  1:426969/1‑249 (MQ=255)                                                                                                                                                                                                                                 ccGGCGGCAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGATATTTCACGCCGGTGTCAATCCACGCTAAGGCTTTCGCGTCCAACACTTGGCTTAAACCGTGCGCCGCAAACGCACCGATAGTCACACATAAAAAACCGCTCACGGCGGcaatc                                                   <  1:15836/210‑1 (MQ=255)                                                                                                                                                                                                                                   ggcggcAACATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGATATTTCACGCCGGTGTCAATCCACGCTAAGGCTTTCGCGTCCAACACTTGGCTTAAACCGTGCGCCGCAAACGCACCGATAGTCACACATAAAAAACCGCTCACGGCGGCAATCAATAACCATTTGTTTTTCATTTAAAAt                        >  1:559134/1‑235 (MQ=255)                                                                                                                                                                                                                                         aaCATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGATATTTCACGCCGGTGTCAATCCACGCTAAGGCTTTCGCGTCCAACACTTGGCTTAAACCGTGCGCCGCAAACGCACCGATAGTCACACATAAAAAACCGCTCACGGCGGCAATCAATAACCATTTGTTTTTCATTTAAAATTTTCCTATTAATAATGCCaaaa  <  1:132288/251‑1 (MQ=255)                                                                                                                                                                                                                                          aCATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGATATTTCACGCCGGTGTCAATCCACGCTAAGGCTTTCGCGTCCAACACTTGGCTTAAACCGTGCGCCGCAAACGCACCGATAGTCACACATAAAAAACCGCTCACGGCGGCAATCAATAAc                                             <  1:865780/207‑1 (MQ=255)                                                                                                                                                                                                                                           cATTGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGATATTTCACGCCGGTGTCAATCCACGCTAAGGCTTTCGCGTCCAACACTTGGCTTAAACCGTGCGCCGCAAACGCACCGATAGTCACACATAAAAAACCGCTCACGGCGGCAATCAATAACCATTTGTTTTTCATTTAAAATTTTCCTATtaat            >  1:839006/1‑239 (MQ=255)                                                                                                                                                                                                                                             ttGGTGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGATATTTCACGCCGGTGTCAATCCACGCTAAGGCTTTCGCGTCCAACACTTGGCTTAAAc                                                                                                           <  1:274164/142‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                   TCATTCAGTTGTGTGAAAAGGGATTGTTGTAAAGGATTAAGTTCTTGTTTTATCGTTCTTTTATTCATTCTGATTTACTTTTGAAAGCGCCGTAAGCTAAACCAAGCCAGCCGATTAAAAATAGCGTGCCGCCGATGGGCGTGAGCCAAACCATAAATTTACCTGAGCCAAGTGCGAGGGCGTATAAACTGCCGCTAAAGAGAAGGATTCCGAATGACCACGCGCCGGCGGCAACATTGGCGCTTTTGTTGGCGGCAAATGGTTCGCGACATAATTGTGCGATGCCGATTCCCATGATGGCGAGGGTGTGGAACATTTGATATTTCACGCCGGTGTCAATCCACGCTAAGGCTTTCGCGTCCAACACTTGGCTTAAACCGTGCGCCGCAAACGCACCGATAGTCACACATAAAAAACCGCTCACGGCGGCAATCAATAACCATTTGTTTTTCATTTAAAATTTTCCTATTAATAATGCCAAAA  >  1/1541476‑1541958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑