Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,534,103	G→A	intergenic (‑17981/‑31961)	unknown ← / → unknown	tRNA‑Arg‑CCG/tRNA‑Asn‑GTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,534,103	0	G	A	100.0%	202.4 / NA	71	intergenic (‑17981/‑31961)	unknown/unknown	tRNA‑Arg‑CCG/tRNA‑Asn‑GTT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (42/29);  total (42/29)

CGGGCACAGGACCCGCCCCTACGCGAAAACACAAAAAAACACCTTTGATAGCGCACGCTTCATAGCGCGTGCTTTTAACTTATTGATATTACAGGCACGCGTTAGGAAACGCGCACCAGCAAAATGTAACGTTTTTCAAAATTCAACTGTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGGCGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAAAGGACTGGCGGAAATCCCCGAAATCGTGAAAAAATTAAGCGGTTCCTCGCTCTCTTTGGAAGAAATCAGGCAGCGCTATTTTGATTTCTTCAAACAGCTTTTTGCCACCGTGGATTACCGTTCAATTTTCCGAGCCGACATTCAGCAAATCATCGATTTCGCCAAGCAA  >  1/1533867‑1534345                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                   cgggccaaggccccGCCCCTACGCGAAAACACAAAAAAACACCTTTGATAGCGCACGCTTCATAGCGCGTGCTTTTAACTTATTGATATTACAGGCACGCGTTAGGAAACGCGCACCAGCAAAATGTAACGTTTTTCAAAATTCAACTGTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGa                                                                                                                                                                                                                                       <  1:650319/239‑1 (MQ=255)    gcACAGGACCCGCCCCTACGCGAAAACACAAAAAAACACCTTTGATAGCGCACGCTTCATAGCGCGTGCTTTTAACTTATTGATATTACAGGCACGCGTTAGGAAACGCGCACCAGCAAAATGTAACGTTTTTCAAAATTCAACTGTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCAt                                                                                                                                                                                                                                          <  1:493584/244‑1 (MQ=255)         ggACCCGCCCCTACGCGAAAACACAAAAAAACACCTTTGATAGCGCACGCTTCATAGCGCGTGCTTTTAACTTATTGATATTACAGGCACGCGTTAGGAAACGCGCACCAGCAAAATGTAACGTTTTTCAAAATTCAACTGTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGc                                                                                                                                                                                                                                    <  1:314840/245‑1 (MQ=255)           ccccGCCCCTACGCGAAAACACAAAAAAACACCTTTGATAGCGCACGCTTCATAGCGCGTGCTTTTAACTTATTGATATTACAGGCACGCGTTAGGAAACGCGCACCAGCAAAATGTAACGTTTTTCAAAATTCAACTGTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAgg                                                                                                                                                                                                                            <  1:358358/250‑1 (MQ=255)                ccccTACGCGAAAACACAAAAAAACACCTTTGATAGCGCACGCTTCATAGCGCGTGCTTTTAACTTATTGATATTACAGGCACGCGTTAGGAAACGCGCACCAGCAAAATGTAACGTTTTTCAAAATTCAACTGTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAaca                                                                                                                                                                                                                        >  1:557582/1‑250 (MQ=255)                 cccTACGCGAAAACACAAAAAAACACCTTTGATAGCGCACGCTTCATAGCGCGTGCTTTTAACTTATTGATATTACAGGCACGCGTTAGGAAACGCGCACCAGCAAAATGTAACGTTTTTCAAAATTCAACTGTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACg                                                                                                                                                                                                                      <  1:23576/251‑1 (MQ=255)                   cTACGCGAAAACACAAAAAAACACCTTTGATAGCGCACGCTTCATAGCGCGTGCTTTTAACTTATTGATATTACAGGCACGCGTTAGGAAACGCGCACCAGCAAAATGTAACGTTTTTCAAAATTCAACTGTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGAc                                                                                                                                                                                                                    <  1:608958/251‑1 (MQ=255)                     aCGCGAAAACACAAAAAAACACCTTTGATAGCGCACGCTTCATAGCGCGTGCTTTTAACTTATTGATATTACAGGCACGCGTTAGGAAACGCGCACCAGCAAAATGTAACGTTTTTCAAAATTCAACTGTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCg                                                                                                                                                                                                                  >  1:356294/1‑251 (MQ=255)                                caAAAAAACACCTTTGATAGCGCACGCTTCATAGCGCGTGCTTTTAACTTATTGATATTACAGGCACGCGTTAGGAAACGCGCACCAGCAAAATGTAACGTTTTTCAAAATTCAACTGTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGa                                                                                                                                                                                                                           <  1:433056/231‑1 (MQ=255)                                caAAAAAACACCTTTGATAGCGCACGCTTCATAGCGCGTGCTTTTAACTTATTGATATTACAGGCACGCGTTAGGAAACGCGCACCAGCAAAATGTAACGTTTTTCAAAATTCAACTGTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAAcc                                                                                                                                                                                                        >  1:144159/1‑250 (MQ=255)                                  aaaaaaCACCTTTGATAGCGCACGCTTCATAGCGCGTGCTTTTAACTTATTGATATTACAGGCACGCGTTAGGAAACGCGCACCAGCAAAATGTAACGTTTTTCAAAATTCAACTGTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCg                                                                                                                                                                                                     >  1:377260/1‑251 (MQ=255)                                                   gcgcACGCTTCATAGCGCGTGCTTTTAACTTATTGATATTACAGGCACGCGTTAGGAAACGCGCACCAGCAAAATGTAACGTTTTTCAAAATTCAACTGTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAAccc                                                                                                                                                                                                       >  1:796924/1‑232 (MQ=255)                                                   gcgcACGCTTCATAGCGCGTGCTTTTAACTTATTGATATTACAGGCACGCGTTAGGAAACGCGCACCAGCAAAATGTAACGTTTTTCAAAATTCAACTGTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGt                                                                                                                                                                                    >  1:223842/1‑251 (MQ=255)                                                        cGCTTCATAGCGCGTGCTTTTAACTTATTGATATTACAGGCACGCGTTAGGAAACGCGCACCAGCAAAATGTAACGTTTTTCAAAATTCAACTGTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAg                                                                                                                                                                               >  1:429337/1‑251 (MQ=255)                                                          cTTCATAGCGCGTGCTTTTAACTTATTGATATTACAGGCACGCGTTAGGAAACGCGCACCAGCAAAATGTAACGTTTTTCAAAATTCAACTGTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAgg                                                                                                                                                                                                                            <  1:86226/204‑1 (MQ=255)                                                          cTTCATAGCGCGTGCTTTTAACTTATTGATATTACAGGCACGCGTTAGGAAACGCGCACCAGCAAAATGTAACGTTTTTCAAAATTCAACTGTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGAc                                                                                                                                                                                                                    <  1:208245/212‑1 (MQ=255)                                                          cTTCATAGCGCGTGCTTTTAACTTATTGATATTACAGGCACGCGTTAGGAAACGCGCACCAGCAAAATGTAACGTTTTTCAAAATTCAACTGTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAAc                                                                                                                                                                                                         >  1:392319/1‑223 (MQ=255)                                                          cTTCATAGCGCGTGCTTTTAACTTATTGATATTACAGGCACGCGTTAGGAAACGCGCACCAGCAAAATGTAACGTTTTTCAAAATTCAACTGTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACg                                                                                                                                                                                             <  1:425471/235‑1 (MQ=255)                                                          cTTCATAGCGCGTGCTTTTAACTTATTGATATTACAGGCACGCGTTAGGAAACGCGCACCAGCAAAATGTAACGTTTTTCAAAATTCAACTGTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAgg                                                                                                                                                                              >  1:290672/1‑250 (MQ=255)                                                                  cgcgTGCTTTTAACTTATTGATATTACAGGCACGCGTTAGGAAACGCGCACCAGCAAAATGTAACGTTTTTCAAAATTCAACTGTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCTCGTTAACCCCGCTAGAACGTTCGGAAGTGATAGGCAAAGGAc                                                                                                                                                                      <  1:181659/250‑1 (MQ=255)                                                                      tGCTTTTAACTTATTGATATTACAGGCACGCGTTAGGAAACGCGCACCAGCAAAATGTAACGTTTTTCAAAATTCAACTGTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAAAGGACTGGCg                                                                                                                                                                 <  1:708103/251‑1 (MQ=255)                                                                       gCTTTTAACTTATTGATATTACAGGCACGCGTTAGGAAACGCGCACCAGCAAAATGTAACGTTTTTCAAAATTCAACTGTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGc                                                                                                                                                                             <  1:649322/238‑1 (MQ=255)                                                                        cTTTTAACTTATTGATATTACAGGCACGCGTTAGGAAACGCGCACCAGCAAAATGTAACGTTTTTCAAAATTCAACTGTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCGATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAAccc                                                                                                                                                                                                       >  1:435734/1‑211 (MQ=255)                                                                        cTTTTAACTTATTGATATTACAGGCACGCGTTAGGAAACGCGCACCAGCAAAATGTAACGTTTTTCAAAATTCAACTGTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAAAGGACTGGCGGa                                                                                                                                                               >  1:898868/1‑251 (MQ=255)                                                                               cTTATTGATATTACAGGCACGCGTTAGGAAACGCGCACCAGCAAAATGTAACGTTTTTCAAAATTCAACTGTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAAAGGACTGGCGGAAATccc                                                                                                                                                         >  1:283765/1‑250 (MQ=255)                                                                                     gATATTACAGGCACGCGTTAGGAAACGCGCACCAGCAAAATGTAACGTTTTTCAAAATTCAACTGTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAAAGGACTGGCGGAAATCCCCGAAAt                                                                                                                                                   >  1:380481/1‑250 (MQ=255)                                                                                      atatTACAGGCACGCGTTAGGAAACGCGCACCAGCAAAATGTAACGTTTTTCAAAATTCAACTGTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGt                                                                                                                                                                                                             >  1:482573/1‑191 (MQ=255)                                                                                       tatTACAGGCACGCGTTAGGAAACGCGCACCAGCAAAATGTAACGTTTTTCAAAATTCAACTGTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCaa                                                                                                                                                                           >  1:657025/1‑224 (MQ=255)                                                                                                    cgTTAGGAAACGCGCACCAGCAAAATGTAACGTTTTTCAAAATTCAACTGTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAAAGGACTGGCGGAAATCCCCGAAATCGTGAAAAAATTAAg                                                                                                                                    >  1:695258/1‑250 (MQ=255)                                                                                                    cgTTAGGAAACGCGCACCAGCAAAATGTAACGTTTTTCAAAATTCAACTGTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAAAGGACTGGCGGAAATCCCCGAAATCGTGAAAAAATTAAg                                                                                                                                    >  1:828752/1‑250 (MQ=255)                                                                                                          gAAACGCGCACCAGCAAAATGTAACGTTTTTCAAAATTCAACTGTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATa                                                                                                                                                                                <  1:586450/200‑1 (MQ=255)                                                                                                                        cAAAATGTAACGTTTTTCAAAATTCAACTGTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAAAGGACTGGCGGAAATCCCCGAAATCGTGAAAAAATTAAGCGGTTCCTCGCTCTCTTTGga                                                                                                               <  1:836357/251‑1 (MQ=255)                                                                                                                        cAAAATGTAACGTTTTTCAAAATTCAACTGTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAAAGGACTGGCGGAAATCCCCGAAATCGTGAAAAAATTAAGCGGTTCCTCGCTCTCTTTGga                                                                                                               <  1:625112/251‑1 (MQ=255)                                                                                                                          aaaTGTAACGTTTTTCAAAATTCAACTGTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAAAGGACTGGCGGAAATCCCCGAAATCGTGAAAAAATTAAGCGGTTCCTCGCTCTCTTTGgaag                                                                                                             <  1:695027/251‑1 (MQ=255)                                                                                                                          aaaTGTAACGTTTTTCAAAATTCAACTGTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAAAGGACTGGCGGAAATCCCCGAAATCGTGAAAAAATTAAGCGGTTCCTCGCTCTCTTTGgaag                                                                                                             <  1:720578/251‑1 (MQ=255)                                                                                                                            aTGTAACGTTTTTCAAAATTCAACTGTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAAAGGACTGGCGGAAATCCCCGAAATCGTGAAAAAATTAAGCGGTTCCTCGCTCTCTTTGgaagaa                                                                                                           >  1:35490/1‑251 (MQ=255)                                                                                                                                           aaaTTCAACTGTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAAAGGACTGGCGGAAATCCCCGAAATCGTGAAAAAATTAAGCGGTTCCTCGc                                                                                                                         >  1:749523/1‑222 (MQ=255)                                                                                                                                           aaaTTCAACTGTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAAAGGACTGGCGGAAATCCCCGAAATCGTGAAAAAATTAAGCGGTTCCTCGCTCTCTTTGGAAGAAATCAGGCAGCGCTAt                                                                                            >  1:51297/1‑251 (MQ=255)                                                                                                                                                     gTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAAc                                                                                                                                                                                                         >  1:335159/1‑132 (MQ=255)                                                                                                                                                     gTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAAAGGACTGGCGGAAATc                                                                                                                                                           >  1:186637/1‑178 (MQ=255)                                                                                                                                                     gTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAAAGGACTGGCGGAAATCCCCGAAATCGTGAAAAAATTAAGCGGTTCCTCGCTCTCTTTGga                                                                                                               >  1:111367/1‑222 (MQ=255)                                                                                                                                                     gTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAAAGGACTGGCGGAAATCCCCGAAATCGTGAAAAAATTAAGCGGTTCCTCGCTCTCTTTGGAAGAAATCAGGc                                                                                                    <  1:227887/233‑1 (MQ=255)                                                                                                                                                         tAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAAAGGACTGGCGGAAATCCCCGAAATCGTGAAAAAATTAAGCGGTTCCTCGCTCTCTTTGGAAGAAATCAGGCAGCGCTATTTTGATTTCTTCaa                                                                              >  1:785884/1‑251 (MQ=255)                                                                                                                                                         tAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAAAGGACTGGCGGAAATCCCCGAAATCGTGAAAAAATTAAGCGGTTCCTCGCTCTCTTTGGAAGAAATCAGGCAGCGCTATTTTGATTTCTTCaa                                                                              >  1:851986/1‑251 (MQ=255)                                                                                                                                                         tAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAAAGGACTGGCGGAAATCCCCGAAATCGTGAAAAAATTAAGCGGTTCCTCGCTCTCTTTGGAAGAAATCAGGCAGCGCTATTTTGATTTCTTCaa                                                                              >  1:102330/1‑251 (MQ=255)                                                                                                                                                           aGGAAACTCTATGCTACAAGCTATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAAAGGACTGGCGGAACTCCCCGAAATGGGGAAAAAATTAAGCGGTTCCTCGCCCTCTTTGGAAGAAATCAGGCAGGGCAAttt                                                                                          >  1:434741/1‑237 (MQ=255)                                                                                                                                                                aCTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAAAGGACTGGCGGAAATCCCCGAAATCGTGAAAAAATTAAGCGGTTCCTCGCTCTCTTTGGAAGAAATCAGGCAGCGCTATTTTGATTTCTTCAAACAGCtt                                                                       >  1:707480/1‑251 (MQ=255)                                                                                                                                                                 ctctATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCAt                                                                                                                                                                                                                                          >  1:497295/1‑87 (MQ=255)                                                                                                                                                                 ctctATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAAAGGACTGGCgg                                                                                                                                                                >  1:689891/1‑161 (MQ=255)                                                                                                                                                                 ctctATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAAAGGACTGGCGGAAATCCCCAAAATCGTGAAAAAATTAAGCGGTTCCTCGCTCTCTTTGGAAGAAATCAGGCAGCGCTATTTTGATTTCTTCAAACAGCttt                                                                      >  1:428873/1‑251 (MQ=255)                                                                                                                                                                 ctctATGCTACAAGCGATCATCTTTGATATGGACGCCGTCTTTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAAAGGACTGGCGGAAATCCCCGAAATCGTGAAAAAATTAAGCGGTTTCTTGCTCTCTTTGGAAGAAATCAGGc                                                                                                    >  1:706697/1‑221 (MQ=255)                                                                                                                                                                   ctATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAAccc                                                                                                                                                                                                       <  1:714923/120‑1 (MQ=255)                                                                                                                                                                      tGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAAAGGACTGGCGGAAATCCCCGAAATCGTGAAAAAATTAAGCGGTTCCTCGCTCTCTTTGGAAGAAATCAGGCAGCGCTATTTTGATTTCTTCAAACAGCTTTTTGcc                                                                 <  1:769514/251‑1 (MQ=255)                                                                                                                                                                       gCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAAAGGACTGGCGGAAATCCCCGAAATCGTGAAAAAATTAAGCGGTTCCTCGCTCTCTTTGgaag                                                                                                             <  1:689772/206‑1 (MQ=255)                                                                                                                                                                        cTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAATCCATTAAGGTACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAATGGACTGGCGGAAATCCCCGAAATCGTGAAAAACTTAAGCGTTTCCTCGCTCTCTTTGTATGAAATCAGGCTGCGCTATTTTGATTTCTTCAAACAGCTTTTTTCCa                                                                >  1:869581/1‑250 (MQ=255)                                                                                                                                                                                  tcatcTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAAAGGACTGGCGGAAATCCCCGAAATCGTGAAAAAATTAAGCGGTTCCTCGCTCTCTTTGGAAGAAATCAGGCAGCGCTATTTTGATTTCTTCAAACAGCTTTTTGCCACCGTGGATTAc                                                     <  1:493070/251‑1 (MQ=255)                                                                                                                                                                                  tcatcTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAAAGGACTGGCGGAAATCCCCGAAATCGTGAAAAAATTAAGCGGTTCCTCGCTCTCTTTGGAAGAAATCAGGCAGCGCTATTTTGATTTCTTCAAACAGCTTTTTGCCACCGTGGATTAc                                                     <  1:177177/251‑1 (MQ=255)                                                                                                                                                                                          gATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAAAGGACTGGCGGAAATCCCCGAAATCGTGAAAAAATTAAGCGGTTCCTCGCTCTCTTTGGAAGAAATCAGGCAGCGCTAttt                                                                                          <  1:780163/206‑1 (MQ=255)                                                                                                                                                                                          gATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAAAGGACTGGCGGAAATCCCCGAAATCGTGAAAAAATTAAGCGGTTCCTCGCTCTCTTTGGAAGAAATCAGGCAGCGCTATTTTGATTTCTTCAAACAGCTTTTTGCCACCGTGGATTACCGTTCAAt                                             >  1:368693/1‑251 (MQ=255)                                                                                                                                                                                          gATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAAAGGACTGGCGGAAATCCCCGAAATCGTGAAAAAATTAAGCGGTTCCTCGCTCTCTTTGGAAGAAATCAGGCAGCGCTATTTTGATTTCTTCAAACAGCTTTTTGCCACCGTGGATTACCGTTCAAt                                             >  1:503057/1‑251 (MQ=255)                                                                                                                                                                                          gATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAAAGGACTGGCGGAAATCCCCGAAATCGTGAAAAAATTAAGCGGTTCCTCGCTCTCTTTGGAAGAAATCAGGCAGCGCTATTTTGATTTCTTCAAACAGCTTTTTGCCACCGTGGATTACCGTTCAAt                                             >  1:665589/1‑251 (MQ=255)                                                                                                                                                                                                gACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAAAGGACTGGCGGAAATCCCCGAAATCGTGAAAAAATTAAGCGGTTCCTCGCTCTCTTTGGAAGAAATCAGGCAGCGCTATTTTGATTTCTTCAAACAGCTTTTTGCCACCGTGGATTACCGTTCAATTTTCCg                                       >  1:108046/1‑251 (MQ=255)                                                                                                                                                                                                       tCATTGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAAAGGACTGGCGGAAATCCCCGAAATCGTGAAAAAATTAAGCGGTTCCTCGCTCTCTTTGGAAGAAATCAGGCAGCGCTATTTTGATTTCTTCAAACAGCTTTTTGCCACCGTGGATTACCGTTCAATTTTCCGAGCCGa                                 >  1:688732/1‑250 (MQ=255)                                                                                                                                                                                                           tGTCGATACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAAAGGACTGGCGGAAATCCCCGAAATCGTGAAAAAATTAAGCGGTTCCTCGCTCTCTTTGGAAGAAATCAGGCAGCGCTATTTTGATTTCTTCAAACAGCTTTTTGCCACCGTGGATTACCGTTCAATTTTCCGAGCCGACAtt                             >  1:247/1‑250 (MQ=255)                                                                                                                                                                                                                aTACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAAAGGACTGGCGGAAATCCCCGAAATCGTGAAAAAATTAAGCGGTTCCTCGCTCTCTTTGGAAGAAATCAGGCAGCGCTATTTTGATTTCTTCAAACAGCTTTTTGCCACCGTGGATTACCGTTCAATTTTCCGAGCCGACATTCAGCaa                       >  1:491663/1‑251 (MQ=255)                                                                                                                                                                                                                 tACCGAATTTTTAGAATACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAAAGGACTGGCGGAAATCCCCGAAATCGTGAAAAAATTAAGCGGTTCCTCGCTCTCTTTGGAAGAAATCAGGCAGCGCTATTTTGATTTCTTCAAACAGCTTTTTGCCACCGTGGATTACCGTTCAATTTTCCGAGCCGACATTCAGCaaa                      <  1:495415/251‑1 (MQ=255)                                                                                                                                                                                                                               aaTACGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAAAGGACTGGCGGAAATCCCCGAAATCGTGAAAAAATTAAGCGGTTCCTCGCTCTCTTTGGAAGAAATCAGGCAGCGCTATTTTGATTTCTTCAAACAGCTTTTTGCCACCGTGGATTACCGTTCAATTTTCCGAGCCGACATTCAGCAAATCATCGATTTCGcc        <  1:858902/251‑1 (MQ=255)                                                                                                                                                                                                                                   cGACTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAAccc                                                                                                                                                                                                       >  1:407691/1‑56 (MQ=255)                                                                                                                                                                                                                                     aCTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAAccc                                                                                                                                                                                                       <  1:840254/54‑1 (MQ=255)                                                                                                                                                                                                                                     aCTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAAAGGACTGGCGGAAATCCCCGAAATCGTGAAAAAATTAAGCGGTTCCTCGCTCTCTTTGGAAGAAATCAGGCAGCGCTATTTTGATTTCTTCAAACAGCTTTTTGCCACCGTGGATTACCGTTCAATTTTCCGAGCCGACATTCAGCAAATCATCGATTTCGCCAAGCaa  >  1:205939/1‑251 (MQ=255)                                                                                                                                                                                                                                     aCTTACAGACGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAAAGGACTGGCGGAAATCCCCGAAATCGTGAAAAAATTAAGCGGTTCCTCGCTCTCTTTGGAAGAAATCAGGCAGCGCTATTTTGATTTCTTCAAACAGCTTTTTGCCACCGTGGATTACCGTTCAATTTTCCGAGCCGACATTCAGCAAATCATCGATTTCGCCAAGCa   <  1:5741/250‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                   CGGGCACAGGACCCGCCCCTACGCGAAAACACAAAAAAACACCTTTGATAGCGCACGCTTCATAGCGCGTGCTTTTAACTTATTGATATTACAGGCACGCGTTAGGAAACGCGCACCAGCAAAATGTAACGTTTTTCAAAATTCAACTGTCATAAGGAAACTCTATGCTACAAGCGATCATCTTTGATATGGACGGCGTCATTGTCGATACCGAATTTTTAGAATACGACTTACAGGCGCAATTCATTGAAGCCATTAAGGAACACGACCGCACGTTAACCCCGCTCGAACGTTCGGAAGTGATAGGCAAAGGACTGGCGGAAATCCCCGAAATCGTGAAAAAATTAAGCGGTTCCTCGCTCTCTTTGGAAGAAATCAGGCAGCGCTATTTTGATTTCTTCAAACAGCTTTTTGCCACCGTGGATTACCGTTCAATTTTCCGAGCCGACATTCAGCAAATCATCGATTTCGCCAAGCAA  >  1/1533867‑1534345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑