Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,521,160	G→A	intergenic (‑5038/‑44904)	unknown ← / → unknown	tRNA‑Arg‑CCG/tRNA‑Asn‑GTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,521,160	0	G	A	100.0%	237.7 / NA	78	intergenic (‑5038/‑44904)	unknown/unknown	tRNA‑Arg‑CCG/tRNA‑Asn‑GTT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (50/28);  total (50/28)

CCTGACAGAAATCCGTTTTCGACCAAAAATCCGATTGTTGCACCAAATTCACCAGCGCTTTAAACACATTACGGTTGCCGCATAAATAGCGCACTTCCAGCAAATTGGTGGCGATGGTAATGTCCGCCAGCCCCTCCGTTTTACATTCTTCAAGAGTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCGGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAATCGTCGGCGAATTGAAAATGTCGCCACAAACGGGCGAGTAATTGATCGTAAAACGCGGTGCGGTTGGCGATGAATTGATCCACGGCTATGTCATCGAAATTCGCCAGTTCAAACTGCTTTAAATTTTCTTTTTGAATTTTGACCGCACTTGG  >  1/1520919‑1521405                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      ccTGACAGAAATCCGTTTTCGACCAAAAATCCGATTGTTGCACCAAATTCACCAGCGCTTTAAACGCATTACGGTTGCCGCATAAATAGCGCACTTCCAGCAAATTGGTGGCGATGGTAATGTCCGCCAGCCCCTCCGTTTTACATTCTTCAAGAGTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTc                                                                                                                                                                                                                                               <  1:114209/250‑1 (MQ=255)     aCAGAAATCCGTTTTCGACCAAAAATCCGATTGTTGCACCAAATTCACCAGCGCTTTAAACACATTACGGTTGCCGCATAAATAGCGCACTTCCAGCAAATTGGTGGCGATGGTAATGTCCGCCAGCCCCTCCGTTTTACATTCTTCAAGAGTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGtta                                                                                                                                                                                                                                          >  1:398476/1‑251 (MQ=255)      cAGAAATCCGTTTTCGACCAAAAATCCGATTGTTGCACCAAATTCACCAGCGCTTTAAACACATTACGGTTGCCGCATAAATAGCGCACTTCCAGCAAATTGGTGGCGATGGTAATGTCCGCCAGCCCCTCCGTTTTACATTCTTCAAGAGTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGttaa                                                                                                                                                                                                                                         >  1:542258/1‑251 (MQ=255)                        cAAAAATCCGATTGTTGCACCAAATTCACCAGCGCTTTAAACACATTACGGTTGCCGCATAAATAGCGCACTTCCAGCAAATTGGTGGCGATGGTAATGTCCGCCAGCCCCTCCGTTTTACATTCTTCAAGAGTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATc                                                                                                                                                                                                                       <  1:528483/251‑1 (MQ=255)                                          aCCAAATTCACCAGCGCTTTAAACACATTACGGTTGCCGCATAAATAGCGCACTTCCAGCAAATTGGTGGCGATGGTAATGTCCGCCAGCCCCTCCGTTTTACATTCTTCAAGAGTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTtc                                                                                                                                                                                                     <  1:164732/251‑1 (MQ=255)                                             aaaTTCACCAGCGCTTTAAACACATTACGGTTGCCGCATAAATAGCGCACTTCCAGCAAATTGGTGGCGATGGTAATGTCCGCCAGCCCCTCCGTTTTACATTCTTCAAGAGTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGttaaaat                                                                                                                                                                                                                                      <  1:812986/215‑1 (MQ=255)                                             aaaTTCACCAGCGCTTTAAACACATTACGGTTGCCGCATAAATAGCGCACTTCCAGCAAATTGGTGGCGATGGTAATGTCCGCCAGCCCCTCCGTTTTACATTCTTCAAGAGTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTtcct                                                                                                                                                                                                   >  1:311483/1‑250 (MQ=255)                                             aaaTTCACCAGCGCTTTAAACACATTACGGTTGCCGCATAAATAGCGCACTTCCAGCAAATTGGTGGCGATGGTAATGTCCGCCAGCCCCTCCGTTTTACATTCTTCAAGAGTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTtcct                                                                                                                                                                                                   >  1:281707/1‑250 (MQ=255)                                               aTTCACCAGCGCTTTAAACACATTACGGTTGCCGCATAAATAGCGCACTTCCAGCAAATTGGTGGCGATGGTAATGTCCGCCAGCCCCTCCGTTTTACATTCTTCAAGAGTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTtcctcc                                                                                                                                                                                                 >  1:94858/1‑250 (MQ=255)                                                         gcTTTAAACACATTACGGTTGCCGCATAAATAGCGCACTTCCAGCAAATTGGTGGCGATGGTAATGTCCGCCAGCCCCTCCGTTTTACATTCTTCAAGAGTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAgc                                                                                                                                                                                       >  1:573597/1‑250 (MQ=255)                                                          cTTTAAACACATTACGGTTGCCGCATAAATAGCGCACTTCCAGCAAATTGGTGGCGATGGTAATGTCCGCCAGCCCCTCCGTTTTACATTCTTCAAGAGTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTtaaaattaaaat                                                                                                                                                                                                                                >  1:849321/1‑208 (MQ=255)                                                          cTTTAAACACATTACGGTTGCCGCATAAATAGCGCACTTCCAGCAAATTGGTGGCGATGGTAATGTCCGCCAGCCCCTCCGTTTTACATTCTTCAAGAGTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAgccg                                                                                                                                                                                     >  1:106537/1‑251 (MQ=255)                                                                acacATTACGGTTGCCGCATAAATAGCGCACTTCCAGCAAATTGGTGGCGATGGTAATGTCCGCCAGCCCCTCCGTTTTACATTCTTCAAGAGTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCAc                                                                                                                                                                                >  1:217085/1‑250 (MQ=255)                                                                   caTTACGGTTGCCGCATAAATAGCGCACTTCCAGCAAATTGGTGGCGATGGTAATGTCCGCCAGCCCCTCCGTTTTACATTCTTCAAGAGTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGttaaaattaaaa                                                                                                                                                                                                                                 >  1:664452/1‑198 (MQ=255)                                                                   caTTACGGTTGCCGCATAAATAGCGCACTTCCAGCAAATTGGTGGCGATGGTAATGTCCGCCAGCCCCTCCGTTTTACATTCTTCAAGAGTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATc                                                                                                                                                                                                                       <  1:684637/208‑1 (MQ=255)                                                                   caTTACGGTTGCCGCATAAATAGCGCACTTCCAGCAAATTGGTGGCGATGGTAATGTCCGCCAGCCCCTCCGTTTTACATTCTTCAAGAGTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGaaa                                                                                                                                                                                                                  >  1:144303/1‑213 (MQ=255)                                                                   caTTACGGTTGCCGCATAAATAGCGCACTTCCAGCAAATTGGTGGCGATGGTAATGTCCGCCAGCCCCTCCGTTTTACATTCTTCAAGAGTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCa                                                                                                                                                                            >  1:836513/1‑251 (MQ=255)                                                                   caTTACGGTTGCCGCATAAATAGCGCACTTCCAGCAAATTGGTGGCGATGGTAATGTCCGCCAGCCCCTCCGTTTTACATTCTTCAAGAGTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCa                                                                                                                                                                            >  1:332252/1‑251 (MQ=255)                                                                       aCGGTTGCCGCATAAATAGCGCACTTCCAGCAAATTGGTGGCGATGGTAATGTCCGCCAGCCCCTCCGTTTTACATTCTTCAAGAGTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATaa                                                                                                                                                                        <  1:77942/251‑1 (MQ=255)                                                                              ccGCATAAATAGCGCACTTCCAGCAAATTGGTGGCGATGGTAATGTCCGCCAGCCCCTCCGTTTTACATTCTTCAAGAGTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCaa                                                                                                                                                                 >  1:765422/1‑251 (MQ=255)                                                                               cGCATAAATAGCGCACTTCCAGCAAATTGGTGGCGATGGTAATGTCCGCCAGCCCCTCCGTTTTACATTCTTCAAGAGTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGgt                                                                                                                                                                                                                                                    <  1:502882/167‑1 (MQ=255)                                                                               cGCATAAATAGCGCACTTCCAGCAAATTGGTGGCGATGGTAATGTCCGCCAGCCCCTCCGTTTTACATTCTTCAAGAGTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGc                                                                                                                                                                     <  1:856734/246‑1 (MQ=255)                                                                               cGCATAAATAGCGCACTTCCAGCAAATTGGTGGCGATGGTAATGTCCGCCAGCCCCTCCGTTTTACATTCTTCAAGAGTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCaa                                                                                                                                                                 >  1:565633/1‑250 (MQ=255)                                                                                 cATAAATAGCGCACTTCCAGCAAATTGGTGGCGATGGTAATGTCCGCCAGCCCCTCCGTTTTACATTCTTCAAGAGTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGttaaaattaaaa                                                                                                                                                                                                                                 <  1:720439/184‑1 (MQ=255)                                                                                                                cGATGGTAATGTCCGCCAGCCCCTCCGTTTTACATTCTTCAAGAGTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGgt                                                                                                                                                                                                                                                    <  1:761020/134‑1 (MQ=255)                                                                                                                cGATGGTAATGTCCGCCAGCCCCTCCGTTTTACATTCTTCAAGAGTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAATCGTCGGCGAATTGAAAATGTCGCca                                                                                                                                >  1:485503/1‑250 (MQ=255)                                                                                                                  aTGGTAATGTCCGCCAGCCCCTCCGTTTTACATTCTTCAAGAGTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATaa                                                                                                                                                                        >  1:762434/1‑208 (MQ=255)                                                                                                                    ggTAATGTCCGCCAGCCCCTCCGTTTTACATTCTTCAAGAGTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGccc                                                                                                                                                                                  >  1:126973/1‑196 (MQ=255)                                                                                                                    ggTAATGTCCGCCAGCCCCTCCGTTTTACATTCTTCAAGAGTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCAc                                                                                                                                                                                >  1:334234/1‑198 (MQ=255)                                                                                                                                   ccccTCCGTTTTACATTCTTCAAGAGTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCgg                                                                                                                                                                                                                                             <  1:84058/122‑1 (MQ=255)                                                                                                                                        ccGTTTTACATTCTTCAAGAGTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAAt                                                                                                                                                         <  1:134854/201‑1 (MQ=255)                                                                                                                                        ccGTTTTACATTCTTCAAGAGTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAATCGTCGGCGAATTGAAAATGTCGCCACAAACGGGCGAGTAATTGATCGTaa                                                                                                       >  1:502994/1‑251 (MQ=255)                                                                                                                                          gTTTTACATTCTTCAAGAGTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGc                                                                                                                                                                             >  1:331033/1‑179 (MQ=255)                                                                                                                                          gTTTTACATTCTTCAAGAGTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAATCGTCGGCGAATTGAAAATGTCGCCACAAACGGGCGAGTAATTGATCGTaaaa                                                                                                     >  1:78780/1‑251 (MQ=255)                                                                                                                                          gTTTTACATTCTTCAAGAGTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAATCGTCGGCGAATTGAAAATGTCGCCACAAACGGGCGAGTAATTGATCGTaaaa                                                                                                     >  1:383182/1‑251 (MQ=255)                                                                                                                                                  ttcttcAAGAGTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAATCGTCGGCGAATTGAAAATGTCGCcac                                                                                                                               <  1:584505/217‑1 (MQ=255)                                                                                                                                                  ttcttcAAGAGTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAATCGTCGGCGAATTGAAAATGTCGCCACAAACGGGCGAGTAATTGATCGTAAAACgcggtg                                                                                              >  1:542026/1‑250 (MQ=255)                                                                                                                                                    cttcAAGAGTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAATCGTCGGCGAATTGAAAAt                                                                                                                                       >  1:474618/1‑207 (MQ=255)                                                                                                                                                          gagTACGAATACTGTGTCCCACATTAAAACCGCAGTCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAATCGTCGGCGAATTGAAAATGTCGCCACAAACGGGCGAGTAATTGATCGTAAAACGCGGTGCGGTTGGCg                                                                                     >  1:375491/1‑251 (MQ=255)                                                                                                                                                          gagTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAATCGTCGGCGAATTGAAAATGTCGCCACAAACGGGCGAGTAATTGATCGTAAAACGCGGTGCGGTTGGCg                                                                                     >  1:18444/1‑251 (MQ=255)                                                                                                                                                           agTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAATCGTCGGCGAATTGAAAATGTCGCCACAAACGGGCGAGTAATTGATCGTAAAACGCGGTGCGGTTGGCGa                                                                                    >  1:865380/1‑251 (MQ=255)                                                                                                                                                            gTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTtaaaattaaaat                                                                                                                                                                                                                                >  1:68742/1‑110 (MQ=255)                                                                                                                                                            gTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAATCGTCGGCGAATTGAAAATGTCGCCACAAACGGGCGAGTAATTGATCGTAAAACGCGGTGCGGTTGGc                                                                                      >  1:492430/1‑248 (MQ=255)                                                                                                                                                            gTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAATCGTCGGCGAATTGAAAATGTCGCCACAAACGGGCGAGTAATTGATCGTAAAACGCGGTGCGGTTGGCGAt                                                                                   >  1:627852/1‑251 (MQ=255)                                                                                                                                                            gTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAATCGTCGGCGAATTGAAAATGTCGCCACAAACGGGCGAGTAATTGATCGTAAAACGCGGTGCGGTTGGCGAt                                                                                   >  1:169519/1‑251 (MQ=255)                                                                                                                                                            gTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAATCGTCGGCGAATTGAAAATGTCGCCACAAACGGGCGAGTAATTGATCGTAAAACGCGGTGCGGTTGGCGAt                                                                                   >  1:223904/1‑251 (MQ=255)                                                                                                                                                               cGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAATCGTCGGCGAATTGAAAATGTCGCCACAAACGGGCGAGTAATTGATCGTAAAACGCGGTGCGGTTGGCGATGaa                                                                                >  1:83925/1‑251 (MQ=255)                                                                                                                                                               cGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAATCGTCGGCGAATTGAAAATGTCGCCACAAACGGGCGAGTAATTGATCGTAAAACGCGGTGCGGTTGGCGATGaa                                                                                >  1:251285/1‑251 (MQ=255)                                                                                                                                                                     cTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAATCGTCGGCGAATTGAAAATGTCGCCACAAACGGGCGAGTAATTGATCGTAAAACGCGGTGCGGTTGGCGATGAATTGAt                                                                           >  1:611101/1‑250 (MQ=255)                                                                                                                                                                     cTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAATCGTCGGCGAATTGAAAATGTCGCCACAAACGGGCGAGTAATTGATCGTAAAACGCGGTGCGGTTGGCGATGAATTGATc                                                                          <  1:542598/251‑1 (MQ=255)                                                                                                                                                                     cTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAATCGTCGGCGAATTGAAAATGTCGCCACAAACGGGCGAGTAATTGATCGTAAAACGCGGTGCGGTTGGCGATGAATTGATc                                                                          <  1:289860/251‑1 (MQ=255)                                                                                                                                                                       gtgtCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAAc                                                                                                                                                                                                          >  1:856117/1‑121 (MQ=255)                                                                                                                                                                       gtgtCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAATCGTCGGCGAATTGAAAATGTCGCCACAAACGGGCGAGTAATTGATCGTAAAACGCGGTGCGGTTGGCGAt                                                                                   <  1:128537/240‑1 (MQ=255)                                                                                                                                                                       gtgtCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAATCGTCGGCGAATTGAAAATGTCGCCACAAACGGGCGAGTAATTGATCGTAAAACGCGGTGCGGTTGGCGATGAATTGATCCa                                                                        >  1:242482/1‑251 (MQ=255)                                                                                                                                                                           cccACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAATCGTCGGCGAATTGAAAATGTCGCCACAAACGGGCGAGTAATTGATCGTAAAACGCGGTGCGGTTGGCGATGAATTGATCCACGGc                                                                    <  1:114952/251‑1 (MQ=255)                                                                                                                                                                               caTTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGgttg                                                                                                                                                                                                                                                  >  1:72580/1‑73 (MQ=255)                                                                                                                                                                               caTTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAATCGTCGGCGAATTGAAAATGTCGCCACAAACGGGCGAGTAATTGATCGTAAAACGCGGTGCGGTTGGCGATGAATTGATCCACGGCTATg                                                                >  1:845403/1‑251 (MQ=255)                                                                                                                                                                                aTTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAATCGTCGGCGAATTGAAAATGTCGCCACAAACGGGCGAGTAATTGATCGTAAAACGCGGTGCGGTTGGCGATGAATTGATCCACGGCTATGt                                                               <  1:618351/251‑1 (MQ=255)                                                                                                                                                                                aTTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAATCGTCGGCGAATTGAAAATGTCGCCACAAACGGGCGAGTAATTGATCGTAAAACGCGGTGCGGTTGGCGATGAATTGATCCACGGCTATGt                                                               <  1:674181/251‑1 (MQ=255)                                                                                                                                                                                     aaCCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAATCGTCGGCGAATTGAAAATGTCGCCACAAACGGGCGAGTAATTGATCGTAAAACGCGGTGCGGTTGGCGATGAATTGATCCACGGCTATGTCATCg                                                          >  1:624588/1‑251 (MQ=255)                                                                                                                                                                                              cccATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGt                                                                                                                                                              <  1:190575/142‑1 (MQ=255)                                                                                                                                                                                               ccATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAATCGTCGGCGAATTGAAAATGTCGCCACAAACGGGCGAGTAATTGATCGTAAAACGCGGTGCGGTTGGCGATGAATTGATCCACGGCTATGTCATCGAAATTCGCCa                                                >  1:181532/1‑251 (MQ=255)                                                                                                                                                                                                cATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAAt                                                                                                                                                         <  1:297190/145‑1 (MQ=255)                                                                                                                                                                                                cATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAATCGTCGGCGAATTGAAAATGTCGCCACAAACGGGCGAGTAATTGATCGTAAAACGCGGTGCGGTTGGCGATGAATTGATCCACGGCTATg                                                                >  1:670069/1‑234 (MQ=255)                                                                                                                                                                                                cATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAATCGTCGGCGAATTGAAAATGTCGCCACAAACGGGCGAGTAATTGATCGTAAAACGCGGTGCGGTTGGCGATGAATTGATCCACGGCTATGTCATCGAAATTCGcc                                                 >  1:752389/1‑249 (MQ=255)                                                                                                                                                                                                       aCTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAATCGTCGGCGAATTGAAAATGTCGCCACAAACGGGCGAGTAATTGATCGTAAAACGCGGTGCGGTTGGCGATGAATTGATCCACGGCTATGTCATCGAAATTCGCCAGTTCAAAc                                        <  1:174267/251‑1 (MQ=255)                                                                                                                                                                                                        cTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAATCGTCGGCGAATTGAAAATGTCGCCACAAACGGGCGAGTAATTGATCGTAAAACGCGGTGCGGTTGGCGATGAATTGATCCACGGCTATGTCATCGAAATTCGCCAGTTCaaa                                         >  1:313594/1‑249 (MQ=255)                                                                                                                                                                                                                 ttGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAATCGTCGGCGAATTGAAAATGTCGCCACAAACGGGCGAGTAATTGATCGTAAAACGCGGTGCGGTTGGCGATGAATTGATCCACGGCTATGTCATCGAAATTCGCCAGTTCAAACTGCTTTAAAt                              <  1:786586/251‑1 (MQ=255)                                                                                                                                                                                                                 ttGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAATCGTCGGCGAATTGAAAATGTCGCCACAAACGGGCGAGTAATTGATCGTAAAACGCGGTGCGGTTGGCGATGAATTGATCCACGGCTATGTCATCGAAATTCGCCAGTTCAAACTGCTTTAAAt                              <  1:826134/251‑1 (MQ=255)                                                                                                                                                                                                                 ttGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAATCGTCGGCGAATTGAAAATGTCGCCACAAACGGGCGAGTAATTGATCGTAAAACGCGGTGCGGTTGGCGATGAATTGATCCACGGCTATGTCATCGAAATTCGCCAGTTCAAACTGCTTTAAAt                              <  1:730213/251‑1 (MQ=255)                                                                                                                                                                                                                     cccAGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAATCGTCGGCGAATTGAAAATGTCGCCACAAACGGGCGAGTAATTGATCGTAAAACGCGGTGCGGTTGGCGATGAATTGATCCACGGCTATGTCATCGAAATTCGCCAGTTCAAACTGCTTTAAATTTTc                          <  1:263479/251‑1 (MQ=255)                                                                                                                                                                                                                        aGATTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAATCGTCGGCGAATTGAAAATGTCGCCACAAACGGGCGAGTAATTGATCGTAAAACGCGGTGCGGTTGGCGATGAATTGATCCACGGCTATGTCATCGAAATTCGCCAGTTCAAACTGCTTTAAATTTTCttt                       >  1:737305/1‑251 (MQ=255)                                                                                                                                                                                                                          aTTTTCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGt                                                                                                                                                              <  1:429186/114‑1 (MQ=255)                                                                                                                                                                                                                           ttttCCTGTAGCGCAGGTGTCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAATCGTCGGCGAATTGAAAATGTCGCCACAAACGGGCGAGTAATTGATCGTAAAACGCGGTGCGGTTGGCGATGAATTGATCCACGGCTATGTCATCGAAATTCGCCAGTTCAAACTGTTTTAAATTTTCTTTTTg                    >  1:830968/1‑251 (MQ=255)                                                                                                                                                                                                                                           gtgtCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCCCAACGTAGAATCGTCGGCGAATTGAAAATGTCGCCACAAACGGGCGAGTAATTGATCGTAAAACGCGGCGCGGTTGGCGTTGAATTGATCCACGGCTATGTCATCGAAATTCGCCAGTTCAAACTGCTTTAAATTTTCTTTTTGAATTTTGACTGCACtt    >  1:130343/1‑251 (MQ=255)                                                                                                                                                                                                                                             gtCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAATCGTCGGCGAATTGAAAATGTCGCCACAAACGGGCGAGTAATTGATCGTAAAACGCGGTGCGGTTGGCGATGAATTGATCCACGGCTATg                                                                <  1:206189/189‑1 (MQ=255)                                                                                                                                                                                                                                             gtCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAATCGTCGGCGAATTGAAAATGTCGCCACAAACGGGCGAGTAATTGATCGTAAAACGCGGTGCGGTTGGCGATGAATTGATCCACGGCTATGTCATCGAAATTCGCCAGTTCAAACTGCTTTAAATTTTCTTTTTGAATTTTGACCGCACTTgg  >  1:255242/1‑251 (MQ=255)                                                                                                                                                                                                                                             gtCGCAGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAATCGTCGGCGAATTGAAAATGTCGCCACAAACGGGCGAGTAATTGATCGTAAAACGCGGTGCGGTTGGCGATGAATTGATCCACGGCTATGTCATCGAAATTCGCCAGTTCAAACTGCTTTAAATTTTCTTTTTGAATTTTGACCGCACTTgg  >  1:582140/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      CCTGACAGAAATCCGTTTTCGACCAAAAATCCGATTGTTGCACCAAATTCACCAGCGCTTTAAACACATTACGGTTGCCGCATAAATAGCGCACTTCCAGCAAATTGGTGGCGATGGTAATGTCCGCCAGCCCCTCCGTTTTACATTCTTCAAGAGTACGAATACTGTGTCCCACATTAAAACCGCAATCCCATAAAAACTGCACAAATTGCCCCAGATTTTCCTGTAGCGCAGGTGTCGCGGGTTGTTCGGTTAAAATTAAAATATCCAAATCGGAAAGGGGAAACATTTCCTCCCGCCCGTAGCCGCCCACCGCAATAAGGCTCAACGTAGAATCGTCGGCGAATTGAAAATGTCGCCACAAACGGGCGAGTAATTGATCGTAAAACGCGGTGCGGTTGGCGATGAATTGATCCACGGCTATGTCATCGAAATTCGCCAGTTCAAACTGCTTTAAATTTTCTTTTTGAATTTTGACCGCACTTGG  >  1/1520919‑1521405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑