Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,516,427	G→A	intergenic (‑305/‑49637)	unknown ← / → unknown	tRNA‑Arg‑CCG/tRNA‑Asn‑GTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,516,427	0	G	A	100.0%	150.3 / NA	53	intergenic (‑305/‑49637)	unknown/unknown	tRNA‑Arg‑CCG/tRNA‑Asn‑GTT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/22);  total (31/22)

ATATTAATTTCTATTTAATTTTAGAACGTCTTGCAGCACGGCGCTTCATGCGACGTATCCAGCGGGTAATTTTCCACGCCCGTGTAATAGAGTAAGAATATAAGAAGAACAGAATAATGAAAGCAATAAACATCACCCATTCATTAATGTAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAATGATGTACGTGCAATCGATCCGGACGGAACGGGCTTTTGCCTTTGCGTAAACGACGATAAATAATTGCCACCATATCTACCAACGGAATCGCAATCACCCAAAGGGCGGTCACCGGATTCATCGGATGCCCTTTGCCTTGTGT  >  1/1516279‑1516672                                                                                                                                                     |                                                                                                                                                                                                                                                      atatTAATTTCTATTTAATTTTAGAACGTCTTGCAGCACGGCGCTTCATGCGACGTATCCAGCGGGTAATTTTCCACGCCCGTGTAATAGAGTAAGAATATAAGAAGAACAGAATAATGAAAGCAATAAACATCACCCATTCATTAATATAGTAGATCTCACCCAAAATAc                                                                                                                                                                                                                                 <  1:624491/171‑1 (MQ=255)  tatTAATTTCTATTTAATTTTAGAACGTCTTGCAGCACGGCGCTTCATGCGACGTATCCAGCGGGTAATTTTCCACGCCCGTGTAATAGAGTAAGAATATAAGAAGAACAGAATAATGAAAGCAATAAACATCACCCATTCATTAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAat                                                                                                                                                <  1:151245/251‑1 (MQ=255)   atTAATTTCTATTTAATTTTAGAACGTCTTGCAGCACGGCGCTTCATGCGACGTATCCAGCGGGTAATTTTCCACGCCCGTGTAATAGAGTAAGAATATAAGAAGAACAGAATAATGAAAGCAATAAACATCACCCATTCATTAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAatg                                                                                                                                               >  1:499701/1‑251 (MQ=255)          tCTATTTAATTTTAGAACGTCTTGCAGCACGGCGCTTCATGCGACGTATCCAGCGGGTAATTTTCCACGCCCGTGTAATAGAGTAAGAATATAAGAAGAACAGAATAATGAAAGCAATAAACATCACCCATTCATTAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAATGATGTACg                                                                                                                                        <  1:442800/251‑1 (MQ=255)             aTTTAATTTTAGAACGTCTTGCAGCACGGCGCTTCATGCGACGTATCCAGCGGGTAATTTTCCACGCCCGTGTAATAGAGTAAGAATATAAGAAGAACAGAATAATGAAAGCAATAAACATCACCCATTCATTAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAATGATGTACGTGc                                                                                                                                     <  1:576709/251‑1 (MQ=255)                  aTTTTAGAACGTCTTGCAGCACGGCGCTTCATGCGACGTATCCAGCGGGTAATTTTCCACGCCCGTGTAATAGAGTAAGAATATAAGAAGAACAGAATAATGAAAGCAATAAACATCACCCATTCATTAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAATGATGTACGTGCAATCg                                                                                                                                >  1:805324/1‑251 (MQ=255)                            gTCTTGCAGCACGGCGCTTCATGCGACGTATCCAGCGGGTAATTTTCCACGCCCGTGTAATAGAGTAAGAATATAAGAAGAACAGAATAATGAAAGCAATAAACATCACCCATTCATTAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAATGATGTACGTGCAATCGATCcggacgg                                                                                                                      >  1:35403/1‑251 (MQ=255)                               ttGCAGCACGGCGCTTCATGCGACGTATCCAGCGGGTAATTTTCCACGCCCGTGTAATAGAGTAAGAATATAAGAAGAACAGAATAATGAAAGCAATAAACATCACCCATTCATTAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAATGATGTACGTGCAATCGATCCGGACGGaa                                                                                                                    >  1:34680/1‑250 (MQ=255)                                          cgcTTCATGCGACGTATCCAGCGGGTAATTTTCCACGCCCGTGTAATAGAGTAAGAATATAAGAAGAACAGAATAATGAAAGCAATAAACATCACCCATTCATTAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAATGATGTACGTGCAATCGATCCGGACGGAACGGGCTTTTGcc                                                                                                        <  1:566933/251‑1 (MQ=255)                                           gcTTCATGCGACGTATCCAGCGGGTAATTTTCCACGCCCGTGTAATAGAGTAAGAATATAAGAAGAACAGAATAATGAAAGCAATAAACATCACCCATTCATTAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAatga                                                                                                                                              <  1:607389/212‑1 (MQ=255)                                           gcTTCATGCGACGTATCCAGCGGGTAATTTTCCACGCCCGTGTAATAGAGTAAGAATATAAGAAGAACAGAATAATGAAAGCAATAAACATCACCCATTCATTAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAATGATGTACGTGCAATCGATCCGGACGGAACGGGCt                                                                                                              <  1:483486/244‑1 (MQ=255)                                              tCATGCGACGTATCCAGCGGGTAATTTTCCACGCCCGTGTAATAGAGTAAGAATATAAGAAGAACAGAATAATGAAAGCAATAAACATCACCCATTCATTAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAat                                                                                                                                                >  1:760823/1‑207 (MQ=255)                                                       gTATCCAGCGGGTAATTTTCCACGCCCGTGTAATAGAGTAAGAATATAAGAAGAACAGAATAATGAAAGCAATAAACATCACCCATTCATTAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAATGATGTACGTGCAATCGATCCGGACGGAACGGGCt                                                                                                              >  1:838241/1‑232 (MQ=255)                                                                        ttCCACGCCCGTGTAATAGAGTAAGAATATAAGAAGAACAGAATAATGAAAGCAATAAACATCACCCATTCATTAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAAcc                                                                                                                                                                 <  1:346596/164‑1 (MQ=255)                                                                         tCCACGCCCGTGTAATAGAGTAAGAATATAAGAAGAACAGAATAATGAAAGCAATAAACATCACCCATTCATTAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAATGATGTACGTGCAATCGATCCGGACGGAACGGGCTTTTGCCTTTGCGTAAACGACGATAAATAATTGccacc                                                                         <  1:309127/251‑1 (MQ=255)                                                                                          gagTAAGAATATAAGAAGAACAGAATAATGAAAGCAATAAACATCACCCATTCATTAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAATGATGTACGTGCAATCGATCCGGACGGAACGGGCt                                                                                                              <  1:507760/197‑1 (MQ=255)                                                                                          gagTAAGAATATAAGAAGAACAGAATAATGAAAGCAATAAACATCACCCATTCATTAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAATGATGTACGTGCAATCGATCCGGACGGAACGGGCTTTTGCCTTTGCGTAAACGACGATAAATAATTGCCACCATATCTACCAACGGAAt                                                        >  1:708819/1‑251 (MQ=255)                                                                                               ggAATATAAGAAGAACAGAATAATGAAAGCAATAAACATCACCCATTCATTAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAATGATGTACGTGCAATCGATCCGGACGGAACGGGCTTTTGCCTTTGCGTAAACGACGATAAATAATTGCCACCATATCTACCAACGGAATCGCaa                                                   >  1:577562/2‑251 (MQ=255)                                                                                                gAATATAAGAAGAACAGAATAATGAAAGCAATAAACATCACCCATTCATTAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAATGATGTACGTGCAATCGATCCGGACGGAACGGGCTTTTGCCTTTGCGTAAACGACGataaataa                                                                                 <  1:236908/220‑1 (MQ=255)                                                                                                gAATATAAGAAGAACAGAATAATGAAAGCAATAAACATCACCCATTCATTAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAATGATGTACGTGCAATCGATCCGGACGGAACGGGCTTTTGCCTTTGCGTAAACGACGATAAATAATTGCCACCATATCTACCAAc                                                             <  1:88328/240‑1 (MQ=255)                                                                                                gAATATAAGAAGAACAGAATAATGAAAGCAATAAACATCACCCATTCATTAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAATGATGTACGTGCAATCGATCCGGACGGAACGGGCTTTTGCCTTTGCGTAAACGACGATAAATAATTGCCACCATATCTACCAACGGAATCGCAAt                                                  >  1:238810/1‑251 (MQ=255)                                                                                                 aaTATAAGAAGAACAGAATAATGAAAGCAATAAACATCACCCATTCATTAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAATGATGTACGTGCAATCGATCCGGACGGAACGGGCTTTTGCCTTTGCGTAAACGACGATAAATAATTGCCACCATATCTACCAACGGAATCGCAAt                                                  >  1:306540/1‑250 (MQ=255)                                                                                                      aagaagAACAGAATAATGAAAGCAATAAACATCACCCATTCATTAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAAc                                                                                                                                                                                                                >  1:387523/1‑87 (MQ=255)                                                                                                      aagaagAACAGAATAATGAAAGCAATAAACATCACCCATTCATTAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAATGATGTACGTGCAATCGATCCGGACGGAACGGGCTTTTGCCTTTGCGTAAACGACGATAAATAATTg                                                                              >  1:700266/1‑217 (MQ=255)                                                                                                           gaaCAGAATAATGAAAGCAATAAACATCACCCATTCATTAATATAGTAGAtc                                                                                                                                                                                                                                              <  1:101445/52‑1 (MQ=255)                                                                                                            aaCAGAATAATGAAAGCAATAAACATCACCCATTCATTAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAATGATGTACGTGCAATCGATCCGGACGGAACGGGCTTTTGCCTTTGCGTAAACGACGATAAATAATTGCCACCATATCTACCAACGGAATCGCAATCACCCAAAggg                                       <  1:847265/250‑1 (MQ=255)                                                                                                                gAATAATGAAAGCAATAAACATCACCCATTCATTAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAatga                                                                                                                                              >  1:856492/1‑143 (MQ=255)                                                                                                                gAATAATGAAAGCAATAAACATCACCCATTCATTAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAATGATGTACGTGCAATCGATCCGGACGGAACGGGCTTTTGCCTTTGCGTAAACGACGATAAATAATTGCCACCATATCTACCAACGGAATCGCAATCACCCAAAGGGCGGt                                   >  1:222605/1‑250 (MQ=255)                                                                                                                    aatGAAAGCAATAAACATCACCCATTCATTAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAat                                                                                                                                                <  1:355712/137‑1 (MQ=255)                                                                                                                           gCAATAAACATCACCCATTCATTAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATa                                                                                                                                                                                                                  <  1:764379/64‑1 (MQ=255)                                                                                                                           gCAATAAACATCACCCATTCATTAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAATGATGTACGTGCAATCGATc                                                                                                                             >  1:805983/1‑149 (MQ=255)                                                                                                                           gCAATAAACATCACCCATTCATTAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAATGATGTACGTGCAATCGATCCGGACGGAACGGGCTTTTGCCTTTGCGTAAACGACGATAAATAATTGccacca                                                                        >  1:82954/1‑202 (MQ=255)                                                                                                                           gCAATAAACATCACCCATTCATTAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAATGATGTACGTGCAATCGATCCGGACGGAACGGGCTTTTGCCTTTGCGTAAACGACGATAAATAATTGCCACCATATCTACCAACGGAATCGCAATCACCCAAAGGGCGGTCACCGGATTCAt                       >  1:671324/1‑251 (MQ=255)                                                                                                                            cAATAAACATCACCCATTCATTAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCAcc                                                                                                                                                       >  1:655789/1‑122 (MQ=255)                                                                                                                              aTAAACATCACCCATTCATTAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAat                                                                                                                                                >  1:670013/1‑127 (MQ=255)                                                                                                                                 aaCATCACCCATTCATTAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAATGATGTACGTGCAATCGATCCGGACGGAACGGGCTTTTGCCTTTGCGTAAACGACGATAAATAATTGCCACCatat                                                                     >  1:747461/1‑199 (MQ=255)                                                                                                                                 aaCATCACCCATTCATTAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAATGATGTACGTGCAATCGATCCGGACGGAACGGGCTTTTGCCTTTGCGTAAACGACGATAAATAATTGCCACCATATCTACCAACGGAATCGCAATCACCCAAAGGGCGGTCACCGGATTCATCGGATg                 >  1:564327/1‑251 (MQ=255)                                                                                                                                  aCATCACCCATTCATTAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAATGATGTACGTGCAATCGATCCGGACGGAACGGGCTTTTGCCTTTGCGTAAACGACGATAAATAATTGCCACCATATCTACCAACGGAATCGCAATCACCCAAAGGGCGGTCACCGGATTCATCGGATg                 >  1:13368/1‑250 (MQ=255)                                                                                                                                         ccATTCATTAATATAGTAGATCTCACCCAAAATTCCGACCCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAATGATGTACGTGCAATCGATCCGGACGGAACGGGCTTTTGCCTTTGCGTAAACGACGATAAATAATTGCCACCATATCTACCAACGGAATCGCAATCACCCAAAGGGCGGTCACCGGATTCATCGGATGCCCTTTGc         <  1:863557/251‑1 (MQ=255)                                                                                                                                          cattcattAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAATGATGTACGTGCAATCGATCCGGACGGAACGGGCTTTTGCCTTTGCGTAAACGACGATAAATAATTGCCACCATATCTACCAACGGAATCGCAATCACCCAAAGGGCGGTCACCGGATTCATCGGATGCCCtttacc        <  1:647995/251‑4 (MQ=255)                                                                                                                                            ttcattAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAATGATGTACGTGCAATCGATCcggacg                                                                                                                       <  1:862145/138‑1 (MQ=255)                                                                                                                                            ttcattAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAATGATGTACGTGCAATCGATCCGGACGGAACGGGCTTTTGCCTTTGCGTAAACGACGATAAATAATTGCCACCATATCTACCAACGGa                                                          >  1:731256/1‑199 (MQ=255)                                                                                                                                            ttcattAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAATGATGTACGTGCAATCGATCCGGACGGAACGGGCTTTTGCCTTTGCGTAAACGACGATAAATAATTGCCACCATATCTACCAACGGa                                                          <  1:23715/199‑1 (MQ=255)                                                                                                                                            ttcattAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAATGATGTACGTGCAATCGATCCGGACGGAACGGGCTTTTGCCTTTGCGTAAACGACGATAAATAATTGCCACCATATCTACCAACGGAATCGCAATCAccc                                             <  1:449928/212‑1 (MQ=255)                                                                                                                                            ttcattAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAATGATGTACGTGCAATCGATCCGGACGGAACGGGCTTTTGCCTTTGCGTAAACGACGATAAATAATTGCCACCATATCTACCAACGGAATCGCAATCACCCAAAGGGCGGTCACCGGATTCATCGGATGCCCTTTGCCtt      >  1:747566/1‑251 (MQ=255)                                                                                                                                              cattAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAATGATGTACGTGCAATCGATCCGGACGGAACGGGCTTTTGCCTTTGCGTAAACGACGATAaataat                                                                                >  1:121585/1‑175 (MQ=255)                                                                                                                                              cattAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAATGATGTACGTGCAATCGATCCGGACGGAACGGGCTTTTGCCTTTGCGTAAACGACGATAAATAATTGCCACCATATCTACCAACGGAATCGCAATCACCCAAAGGGCGGTCAcc                               >  1:757186/1‑224 (MQ=255)                                                                                                                                              cattAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAATGATGTACGTGCAATCGATCCGGACGGAACGGGCTTTTGCCTTTGCGTAAACGACGATAAATAATTGCCACCATATCTACCAACGGAATCGCAATCACCCAAAGGGCGGTCACCGGATTCATCGGATGCCCTTTGcc        >  1:216274/1‑247 (MQ=255)                                                                                                                                              cattAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAATGATGTACGTGCAATCGATCCGGACGGAACGGGCTTTTGCCTTTGCGTAAACGACGATAAATAATTGCCACCATATCTACCAACGGAATCGCAATCACCCAAAGGGCGGTCACCGGATTCATCGGATGCCCTTTGCCTtgt    >  1:10561/1‑251 (MQ=255)                                                                                                                                              cattAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAATGATGTACGTGCAATCGATCCGGACGGAACGGGCTTTTGCCTTTGCGTAAACGACGATAAATAATTGCCACCATATCTACCAACGGAATCGCAATCACCCAAAGGGCGGTCACCGGATTCATCGGATGCCCTTTGCCTtgt    >  1:840285/1‑251 (MQ=255)                                                                                                                                              cattAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAATGATGTACGTGCAATCGATCCGGACGGAACGGGCTTTTGCCTTTGCGTAAACGACGATAAATAATTGCCACCATATCTACCAACGGAATCGCAATCACCCAAAGGGCGGTCACCGGATTCATCGGATGCCCTTTGCCTtgt    >  1:547031/1‑251 (MQ=255)                                                                                                                                               attAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTTAATGATGTACGTGCAATCGATCCGGACGGAACGGGCTTTTGCCTTTGCGTAAACGACGATAAATAATTGCCACCATATCTACCAACGGAATCGCAATCACCCAAAGGGCGGTCACCGGATTCATCGGATGCCCTTTGCCTtgtg   <  1:108897/251‑1 (MQ=255)                                                                                                                                                ttAATATAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAATGATGTACGTGCAATCGATCCGGACGGAACGGGCTTTTGCCTTTGCGTAAACGACGATAAATAATTGCCACCATATCTACCAACGGAATCGCAATCACCCAAAGGGCGGTCACCGGATTCATCGGATGCCCTTTGCCTtgtgt  >  1:205204/1‑251 (MQ=255)                                                                                                                                                     |                                                                                                                                                                                                                                                      ATATTAATTTCTATTTAATTTTAGAACGTCTTGCAGCACGGCGCTTCATGCGACGTATCCAGCGGGTAATTTTCCACGCCCGTGTAATAGAGTAAGAATATAAGAAGAACAGAATAATGAAAGCAATAAACATCACCCATTCATTAATGTAGTAGATCTCACCCAAAATACCGAACCCGGCACATAACGCCGCCACAAAGGTAATTAATAAAAACGCCTGGCGGGAAGTTAAACCGGCGCGCACCATTAAATGATGTACGTGCAATCGATCCGGACGGAACGGGCTTTTGCCTTTGCGTAAACGACGATAAATAATTGCCACCATATCTACCAACGGAATCGCAATCACCCAAAGGGCGGTCACCGGATTCATCGGATGCCCTTTGCCTTGTGT  >  1/1516279‑1516672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑