Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,515,772	T→C	intergenic (‑94/+88)	unknown ← / ← unknown	SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/tRNA‑Pro‑TGG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,515,772	0	T	C	100.0%	110.5 / NA	38	intergenic (‑94/+88)	unknown/unknown	SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/tRNA‑Pro‑TGG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/16);  total (22/16)

GTGTTAAGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTCAAAAAGCTTAATCCCTCAATAGCTGACTTAATTAATCTATTATAAATAATATAAATAAACGAATCTTCCGGCACCTATTAAGTTCAATCTTAAAATTATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAATTCTGAGAAAACCCAGAATTAGAATTGGTCGGCGAGATAGGATTTGAACCTACGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGATTGGGGTGGCTAATGGGACTTGAACCCACGACAACCGGAATCACAATCCGGGGCTCTACCAACTGAGCTATAGCCACC  >  1/1515622‑1516015                                                                                                                                                       |                                                                                                                                                                                                                                                    gtgtTAAGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTCAAAAAGCTTAATCCCTCAATAGCTGACTTAATTAATCTATTATAAATAATATAAATAGACGAATCTTCCGGCACCTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAATTCTGAGAAAACCCAGAATTAGAATTGGTCGGCGAGAt                                                                                                                                                 >  1:584630/1‑251 (MQ=255)                    cGTTCAATCTGAGCCATGATCAAACTCTTCAATTCAAAAAGCTTAATCCCTCAATAGCTGACTTAATTAATCTATTATAAATAATATAAATAAACGAATCTTCCGGCACCTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAATTCTGAGAAAACCCAGAATTAGAATTGGTCGGCGAGATAGGATTTGAACCTACGAc                                                                                                                               <  1:161940/250‑1 (MQ=255)                                    tGATCAAACTCTTCAATTCAAAAAGCTTAATCCCTCAATAGCTGACTTAATTAATCTATTATAAATAATATAAATAAACGAATCTTCCGGCACCTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAATTCTGAGAAAACCCAGAATTAGAATTGGTCGGCGAGATAGGATTTGAACCTACGACCCACTGGTCCCAAACCa                                                                                                              >  1:813826/1‑251 (MQ=255)                                        cAAACTCTTCAATTCAAAAAGCTTAATCCCTCAATAGCTGACTTAATTAATCTATTATAAATAATATAAATAAACGAATCTTCCGGCACCTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAATTCTGAGAAAACCCAGAATTAGAATTGGTCGGCGAGATAGGATTTGAACCTACGACCCACTGGTCCCAAACCAGTTg                                                                                                          <  1:470007/251‑1 (MQ=255)                                                tcaattcaaAAAGCTTAATCCCTCAATAGCTGACTTAATTAATCTATTATAAATAATATAAATAAACGAATCTTCCGGCACCTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAATTCTGAGAAAACCCAGAATTAGAATTGGTCGGCGAGATAGGATTTGAACCTACGACCCACTGGTCCCAAACCAGTTGCGCTACCa                                                                                                  <  1:773776/251‑1 (MQ=255)                                                     tcaaAAAGCTTAATCCCTCAATAGCTGACTTAATTAATCTATTATAAATAATATAAATAAACGAATCTTCCGGCACCTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAATTCTGAGAAAACCCAGAATTAGAATTGGTCGGCGAGATAGGATTTGAACCTACGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTg                                                                                             <  1:219767/251‑1 (MQ=255)                                                      caaAAAGCTTAATCCCTCAATAGCTGACTTAATTAATCTATTATAAATAATATAAATAAACGAATCTTCCGGCACCTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAATTCTGAGAAAACCCagaatt                                                                                                                                                                   >  1:603230/1‑180 (MQ=255)                                                          aaGCTTAATCCCTCAATAGCTGACTTAATTAATCTATTATAAATAATATAAATAAACGAATCTTCCGGCACCTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAATTCTGAGAAAACCCAGAATTAGAATTGGTCGGCGAGATAGGATTTGAACCTACGACCCACTGGTCCCAAACCAGTTGCGCTAcc                                                                                                   >  1:397822/1‑240 (MQ=255)                                                                     cTCAATAGCTGACTTAATTAATCTATTATAAATAATATAAATAAACGAATCTTCCGGCACCTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAATTCTGAGAAAACCCAGAATTAGAATTGGTCGGCGAGATAGGATTTGAACCTACGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGATTg                                                                             >  1:605021/1‑251 (MQ=255)                                                                       cAATAGCTGACTTAATTAATCTATTATAAATAATATAAATAAACGAATCTTCCGGCACCTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAATTCTGAGAAAACCCAGAATTAGAATTGGTCGGCGAGATAGGATTTGAACCTACGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGATTggg                                                                           >  1:769996/1‑251 (MQ=255)                                                                       cAATAGCTGACTTAATTAATCTATTATAAATAATATAAATAAACGAATCTTCCGGCACCTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAATTCTGAGAAAACCCAGAATTAGAATTGGTCGGCGAGATAGGATTTGAACCTACGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGATTggg                                                                           >  1:744409/1‑251 (MQ=255)                                                                               gACTTAATTAATCTATTATAAATAATATAAATAAACGAATCTTCCGGCACCTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAATTCTGAGAAAACCCAGAATTAGAATTGGTCGGCGAGATAGGATTTGAACCTACGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGATTGGGGTGGCTaa                                                                   >  1:870935/1‑251 (MQ=255)                                                                                   taattaatCTATTATAAATAATATAAATAAACGAATCTTCCGGCACCTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAATTCTGAGAAAACCCAGAATTAGAATTGGTCGGCGAGATAGGAt                                                                                                                                            >  1:318866/1‑174 (MQ=255)                                                                                             attatAAATAATATAAATAAACGAATCTTCCGGCACCTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAATTCTGAGAAAACCCAGAATTAGAATTGGTCGGCGAGATAGGATTTGAACCTACGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGATTgggg                                                                          <  1:348975/230‑1 (MQ=255)                                                                                             attatAAATAATATAAATAAACGAATCTTCCGGCACCTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAATTCTGAGAAAACCCAGAATTAGAATTGGTCGGCGAGATAGGATTTGAACCTACGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGATTGGGGTGGCTAATGGGACTTGAAcc                                                      >  1:342624/1‑250 (MQ=255)                                                                                                     taatataaataaACGAATCTTCCGGCACCTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAATTCTGAGAAAACCCAGAATTAGAATTGGTCGGCGAGATAGGAtt                                                                                                                                           >  1:805384/1‑157 (MQ=255)                                                                                                     taatataaataaACGAATCTTCCGGCACCTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAATTCTGAGAAAACCCAGAATTAGAATTGGTCGGCGAGATAGGATTTGAACCTACGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGATTGGGGTGGCTAATGGGACTTGAACCCACGACaa                                              >  1:218491/1‑250 (MQ=255)                                                                                                           aaataaaCGAATCTTCCGGCACCTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAATTCTGAGAAAACCCAGAATTAGAATTGGTCGGCGAGATAGGATTTGAACCTACGACCCAc                                                                                                                           <  1:203759/167‑1 (MQ=255)                                                                                                                  cGAATCTTCCGGCACCTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAATTCTGAGAAAACCCAGAATTAGAATTGGTCGGCGAGATAGGATTTGAACCTACGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGATTGGGGTGGCTAATGGGACTTGAACCCACGACAACCGGAATCACAATc                                <  1:226679/251‑1 (MQ=255)                                                                                                                       cTTCCGGCACCTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAATTCTGAGAAAACCCAGAATTAGAATTGGTCGGCGAGATAGGATTTGAACCTACGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGATTGGGGTGGCTAATGGGACTTGAACCCACGACAACCGGAATCACAATCCgggg                           >  1:530161/1‑251 (MQ=255)                                                                                                                        ttCCGGCACCTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAATTCTGAGAAAACCCAGAATTAGAATTGGTCGGCGAGATAGGATTTGAACCTACGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGATTgg                                                                            >  1:255893/1‑201 (MQ=255)                                                                                                                        ttCCGGCACCTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAATTCTGAGAAAACCCAGAATTAGAATTGGTCGGCGAGATAGGATTTGAACCTACGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGATTGGGGTgg                                                                       >  1:859397/1‑206 (MQ=255)                                                                                                                         tCCGGCACCTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAATTCTGAGAAAACCCAGAATTAGAATTGGTCGGCGAGATAGGATTTGAACCTACGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGATTGGGGTGGCTAATGGGACTTGAACCCACGACAACCGGAATCACAATCCgggg                           >  1:334893/1‑249 (MQ=255)                                                                                                                         tCCGGCACCTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAATTCTGAGAAAACCCAGAATTAGAATTGGTCGGCGAGATAGGATTTGAACCTACGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGATTGGGGTGGCTAATGGGACTTGAACCCACGACAACCGGAATCACAATCCGGGGc                          <  1:224232/250‑1 (MQ=255)                                                                                                                                ccTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAATTCTGAGAAAACCCAGAATTAGAATTGGTCGGCGAGATAGGATTTGAACCTACGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGATTGGGGTGGCTAATggg                                                               <  1:419958/206‑1 (MQ=255)                                                                                                                                ccTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAATTCTGAGAAAACCCAGAATTAGAATTGGTCGGCGAGATAGGATTTGAACCTACGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGATTGGGGTGGCTAATGGGACTTGAACCCACGACAACCGGAATCACAATCCGGGGCTCTACCaa                  >  1:738451/1‑251 (MQ=255)                                                                                                                                ccTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAATTCTGAGAAAACCCAGAATTAGAATTGGTCGGCGAGATAGGATTTGAACCTACGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGATTGGGGTGGCTAATGGGACTTGAACCCACGACAACCGGAATCACAATCCGGGGCTCTACCaa                  >  1:285628/1‑251 (MQ=255)                                                                                                                                   aTTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAATTCTGAGAAAACCCAGAATTAGAATTGGTCGGCGAGATAGGATTTGAACCTACGAc                                                                                                                               >  1:820150/1‑139 (MQ=255)                                                                                                                                   aTTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAATTCTGAGAAAACCCAGAATTAGAATTGGTCGGCGAGATAGGATTTGAACCTACGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGATTGGGGTGGCTAATgg                                                                <  1:56472/202‑1 (MQ=255)                                                                                                                                   aTTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAATTCTGAGAAAACCCAGAATTAGAATTGGTCGGCGAGATAGGATTTGAACCTACGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGATTGGGGTGGCTAATGGGACTTGAACCCACGACAACCGGAATCACAATCCGGGGCTCTACCaa                  >  1:145799/1‑248 (MQ=255)                                                                                                                                     tAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAATTCTGAGAAAACCCAGAATTAGAATTGGTCGGCGAGATAGGATTTGAACCTACGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGATTGGGGTGGCTAATGGGACTTGAACCCACGACAACCGGAATCACAATCCGGGGCTCTACCAACTGAg             <  1:87781/251‑1 (MQ=255)                                                                                                                                        gTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAATTCTGAGAAAACCCAGAATTAGAATTGGTCGGCGAGATAGGATTTGAACCTACGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGATTggg                                                                           >  1:576738/1‑186 (MQ=255)                                                                                                                                        gTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAATTCTGAGAAAACCCAGAATTAGAATTGGTCGGCGAGATAGGATTTGAACCTACGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGATTGGGGTGGCTAATGGGACTTGAACCCACGACAACCGGAATCACAATCCGGGGCTCTACCAACTGAGc            <  1:412907/249‑1 (MQ=255)                                                                                                                                           cAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAATTCTGAGAAAACCCAGAATTAGAATTGGTCGGCGAGATAGGATTTGAACCTACGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGATTGGGGTGGCTAATGGGACTTGAACCCACGACAACCGGAATCACAATCCGGGGCTCTACCAACTGAGCTATAg       <  1:504088/251‑1 (MQ=255)                                                                                                                                             aTCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAATTCTGAGAAAACCCAGAATTAGAATTGGTCGGCGAGATAGGATTTGAACCTACGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGATTGGGGTGGCTAATGGGACTTGAACCCACGACAACCGGAATCACAATCCGGGGCTCTACCAACTGAGCTATAg       <  1:706993/249‑1 (MQ=255)                                                                                                                                             aTCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAATTCTGAGAAAACCCAGAATTAGAATTGGTCGGCGAGATAGGATTTGAACCTACGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGATTGGGGTGGCTAATGGGACTTGAACCCACGACAACCGGAATCACAATCCGGGGCTCTACCAACTGAGCTATAGcc     <  1:616093/251‑1 (MQ=255)                                                                                                                                              tCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAATTCTGAGAAAACCCAGAATTAGAATTGGTCGGCGAGATAGGATTTGAACCTACGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGATTGGGGTGGCTAATGGGACTTGAACCCACGACAACCGGAATCACAATCCGGGGCTCTACCAACTGAGCTATAGcca    >  1:669715/1‑251 (MQ=255)                                                                                                                                                ttAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAATTCTGAGAAAACCCAGAATTAGAATTGGTCGGCGAGATAGGATTTGAACCTACGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGATTGGGGTGGCTAATGGGACTTGAACCCACGACAACCGGAATCACAATCCGGGGCTCTACCAACTGAGCTATAGccacc  <  1:275741/251‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                                                                                                                    GTGTTAAGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTCAAAAAGCTTAATCCCTCAATAGCTGACTTAATTAATCTATTATAAATAATATAAATAAACGAATCTTCCGGCACCTATTAAGTTCAATCTTAAAATTATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAATTCTGAGAAAACCCAGAATTAGAATTGGTCGGCGAGATAGGATTTGAACCTACGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGATTGGGGTGGCTAATGGGACTTGAACCCACGACAACCGGAATCACAATCCGGGGCTCTACCAACTGAGCTATAGCCACC  >  1/1515622‑1516015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑