Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,509,777	T→C	intergenic (+18153/+14)	unknown → / ← unknown	tRNA‑Ser‑GGA/tRNA‑Trp‑CCA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,509,777	0	T	C	100.0%	18.1 / NA	8	intergenic (+18153/+14)	unknown/unknown	tRNA‑Ser‑GGA/tRNA‑Trp‑CCA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/4);  total (4/4)

TACCGATTAGAGACAATTTTCAGTTTAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGAAAAGGTTACAAAAGTTGGCGGAATGGACGGGACTCGAACCCGCGACCCCCTGCGTGACAGGCAGGTATTCTAACCAGCTGAACTACCACTCCAGAATGAGAATTAGCAGTGACCTACTCTCACATAGG  >  1/1509752‑1510013                          |                                                                                                                                                                                                                                             aaacggatAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGAAAAGGTTACAAAAGTTGGCGGAATGGACGGGACTCGAACCCGCGACCCCCTGCGTGACAGGCAGGTATTCTAACCAGCTGAACTACCACTCCAGAATGAGAATTAGCAGTGACCTAc             <  1:809954/244‑1 (MQ=255)      gatAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGgtatg                                                                                                                                            >  1:650598/3‑119 (MQ=255)      gatAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGAAAAGGTTACAAAAGTTGGCGGAATGGACGGGACTCGAACCCGCGACCCCCTGCGTGACAGGCAGGTATTCTAACCAGCTGAACTACCACTCCAGAATGAGAATTAGCAGTGACCTACTCTca        >  1:106134/3‑251 (MQ=255)      gatAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGAAAAGGTTACAAAAGTTGGCGGAATGGACGGGACTCGAACCCGCGACCCCCTGCGTGACAGGCAGGTATTCTAACCAGCTGAACTACCACTCCAGAATGAGAATTAGCAGTGACCTACTCTca        >  1:427054/3‑251 (MQ=255)           agaCAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGAAAAGGTTACAAAAGTTGGCGGAATGGACGGGACTCGAACCCGCGACCCCCTGCGTGACAGGCAGGTATTCTAACCAGCTGAACTACCACTCCAGAATGAGAATTAGCAGTGACCTACtctc         >  1:659346/1‑245 (MQ=255)             aCAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTAc                                                                                                                                                                       <  1:477242/85‑1 (MQ=255)             aCAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGAAAAGGTTACAAAAGTTGGCGGAATGGACGGGACTCGAACCCGCGACCCCCTGCGTGACAGGCAGGTATTCTAACCAGCTGAACTACCACTCCAGAATGAGAATTAGCAGTGACCTACTCTCACATAgg  <  1:412654/250‑1 (MQ=255)             aCAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGAAAAGGTTACAAAAGTTGGCGGAATGGACGGGACTCGAACCCGCGACCCCCTGCGTGACAGGCAGGTATTCTAACCAGCTGAACTACCACTCCAGAATGAGAATTAGCAGTGACCTACTCTCACATAg   <  1:391733/249‑1 (MQ=255)                          |                                                                                                                                                                                                                                             TACCGATTAGAGACAATTTTCAGTTTAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGAAAAGGTTACAAAAGTTGGCGGAATGGACGGGACTCGAACCCGCGACCCCCTGCGTGACAGGCAGGTATTCTAACCAGCTGAACTACCACTCCAGAATGAGAATTAGCAGTGACCTACTCTCACATAGG  >  1/1509752‑1510013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑