Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,463,370	A→G	intergenic (‑45119/‑28165)	unknown ← / → unknown	SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/tRNA‑Ser‑GGA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,463,370	0	A	G	100.0%	187.9 / NA	67	intergenic (‑45119/‑28165)	unknown/unknown	SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/tRNA‑Ser‑GGA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/27);  total (40/27)

GGCACAGGCAAACATCAGCATTAACGCCATTGCCCAAGGTTCTTCCGAACGTTCAATTTCCGCCGTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCACCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGTTTATACGAACGCGCCTTAGCGGAAGGGTTCCATGTGGTGACTCCGAATAAAAAAGCCAATACC  >  1/1463126‑1463615                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      ggCACAGGCAAACATCAGCATTAACGCCATTGCCCAAGGTTCTTCCGAACGTTCAATTTCCGCCGTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCAGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTcg                                                                                                                                                                                                                                                 >  1:442698/1‑251 (MQ=255)             cATCAGCATTAACGCCATTGCCCAAGGTTCTTCCGAACGTTCAATTTCCGCCGTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCcaaca                                                                                                                                                                                                                                     >  1:299695/1‑251 (MQ=255)              aTCAGCATTAACGCCATTGCCCAAGGTTCTTCCGAACGTTCAATTTCCGCCGTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCcaacaa                                                                                                                                                                                                                                    <  1:861360/251‑1 (MQ=255)                  gCATTAACGCCATTGCCCAAGGTTCTTCCGAACGTTCAATTTCCGCCGTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATg                                                                                                                                                                                                                                >  1:752013/1‑251 (MQ=255)                   cATTAACGCCATTGCCCAAGGTTCTTCCGAACGTTCAATTTCCGCCGTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGt                                                                                                                                                                                                                               <  1:877725/251‑1 (MQ=255)                        aCGCCATTGCCCAAGGTTCTTCCGAACGTTCAATTTCCGCCGTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTc                                                                                                                                                                                                                          <  1:819680/251‑1 (MQ=255)                                gCCCAAGGTTCTTCCGAACGTTCAATTTCCGCCGTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCGAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGaaaa                                                                                                                                                                                                                  >  1:432372/1‑251 (MQ=255)                                gCCCAAGGTTCTTCCGAACGTTCAATTTCCGCCGTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTc                                                                                                                                                                                                                          >  1:662848/1‑243 (MQ=255)                                gCCCAAGGTTCTTCCGAACGTTCAATTTCCGCCGTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGaaaa                                                                                                                                                                                                                  >  1:161909/1‑251 (MQ=255)                                           ttcCGAACGTTCAATTTCCGCCGTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAAtt                                                                                                                                                                                                       <  1:512841/251‑1 (MQ=255)                                            tcCGAACGTTCAATTTCCGCCGTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAAttt                                                                                                                                                                                                      <  1:539376/251‑1 (MQ=255)                                                aaCGTTCAATTTCCGCCGTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGAt                                                                                                                                                                                                  <  1:237355/251‑1 (MQ=255)                                                 aCGTTCAATTTCCGCCGTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATc                                                                                                                                                                                                 <  1:283559/251‑1 (MQ=255)                                                 aCGTTCAATTTCCGCCGTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATc                                                                                                                                                                                                 <  1:289320/251‑1 (MQ=255)                                                             cgccgTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAAt                                                                                                                                                                                                                                            >  1:497614/1‑196 (MQ=255)                                                                            aaaTAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAAtt                                                                                                                                                                                                       >  1:581706/1‑218 (MQ=255)                                                                            aaaTAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCa                                                                                                                                                                      >  1:703874/1‑251 (MQ=255)                                                                                   gCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAAc                                                                                                                                                                >  1:51329/1‑250 (MQ=255)                                                                                     cATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTTTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCCTGCGAATTCCAACAAAATGTTGGTCAATGAAAACGGCTTAAATTTTGATCATTGGTAAACGGACTTAGAAAACGCCACCCaaacg                                                                                                                                                              >  1:231129/1‑247 (MQ=255)                                                                                              cGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCcaac                                                                                                                                                                                                                                      <  1:511247/169‑1 (MQ=255)                                                                                              cGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCg                                                                                                                                                    >  1:239951/1‑251 (MQ=255)                                                                                                  aaaGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGt                                                                                                                                                <  1:84845/251‑1 (MQ=255)                                                                                                     gCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGAc                                                                                                                                                  >  1:792392/1‑246 (MQ=255)                                                                                                     gCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTa                                                                                                                                               >  1:491818/1‑249 (MQ=255)                                                                                                           cACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCg                                                                                                                                                              >  1:891470/1‑228 (MQ=255)                                                                                                              cAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCttt                                                                                                                                    <  1:137050/251‑1 (MQ=255)                                                                                                              cAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCttt                                                                                                                                    <  1:274870/251‑1 (MQ=255)                                                                                                                 gCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCAt                                                                                                                                 >  1:709893/1‑251 (MQ=255)                                                                                                                 gCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGCCGGATTTCGACGTACTGCTCTCTTTCAt                                                                                                                                 >  1:210181/1‑251 (MQ=255)                                                                                                                    ttATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTaa                                                                                                                              >  1:545017/1‑251 (MQ=255)                                                                                                                      aTTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGAt                                                                                                                                                                                                  >  1:302739/1‑181 (MQ=255)                                                                                                                      aTTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGaa                                                                                                                                                                             <  1:88039/202‑1 (MQ=255)                                                                                                                      aTTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAAc                                                                                                                                                                >  1:800440/1‑215 (MQ=255)                                                                                                                            taaCAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGcatc                                                                                                                      <  1:632683/251‑1 (MQ=255)                                                                                                                               cAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGaa                                                                                                                                                                             <  1:336345/193‑1 (MQ=255)                                                                                                                               cAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCAtg                                                                                                                   >  1:361825/1‑251 (MQ=255)                                                                                                                                      aTCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGa                                                                                                             <  1:692739/250‑1 (MQ=255)                                                                                                                                         gTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTg                                                                                                                                                                                                                             >  1:424957/1‑135 (MQ=255)                                                                                                                                               gtgtTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGGCTTAGAAAACGCCACCCAACCGTCGGATTTCGAc                                                                                                                                                  >  1:437277/1‑204 (MQ=255)                                                                                                                                               gtgtTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCttt                                                                                                                                    >  1:172154/1‑218 (MQ=255)                                                                                                                                                   ttCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTg                                                                                               <  1:8451/251‑1 (MQ=255)                                                                                                                                                     cTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCttt                                                                                                                                    >  1:326746/1‑212 (MQ=255)                                                                                                                                                     cTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCAtg                                                                                                                   >  1:394612/1‑229 (MQ=255)                                                                                                                                                           ggtgtcggcggtgtcggcGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTg                                                                                          >  1:66677/1‑248 (MQ=255)                                                                                                                                                             tgtcggcggtgtcggcggCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCttt                                                                                                                                    >  1:168506/1‑204 (MQ=255)                                                                                                                                                                           gcggcgAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACgg                                                                                                                                                                                                               >  1:872614/1‑115 (MQ=255)                                                                                                                                                                              gcgAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGc                                                                     <  1:407087/250‑1 (MQ=255)                                                                                                                                                                               cgAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGcc                                                                    <  1:236874/250‑1 (MQ=255)                                                                                                                                                                                gAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGcat                                                                                                                       >  1:665160/1‑198 (MQ=255)                                                                                                                                                                                 aattaattGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATc                                                                                                                                                                                                 <  1:370041/123‑1 (MQ=255)                                                                                                                                                                                 aattaattGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGt                                                                 >  1:659385/1‑251 (MQ=255)                                                                                                                                                                                 aattaattGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGt                                                                 >  1:787732/1‑251 (MQ=255)                                                                                                                                                                                    taattGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCAtgt                                                                                                                  <  1:468670/199‑1 (MQ=255)                                                                                                                                                                                        tGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCGTTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGTTTATACg                                                          <  1:671305/251‑1 (MQ=255)                                                                                                                                                                                        tGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGTTTATACg                                                          <  1:288547/251‑1 (MQ=255)                                                                                                                                                                                               aTCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGTTTATACGAAcgcg                                                    >  1:391159/1‑250 (MQ=255)                                                                                                                                                                                                       ccAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGTTTATACGAACGCGCCTTAGCgg                                           >  1:340603/1‑251 (MQ=255)                                                                                                                                                                                                            aaGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGTTTATACGAACGCGCCTTAGCGGAAggg                                      <  1:175492/251‑1 (MQ=255)                                                                                                                                                                                                                   tttAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTAGGCCGGTTTATACGAACGCGCCTTAGCGGAAGGGTTCCAtg                               <  1:675233/251‑1 (MQ=255)                                                                                                                                                                                                                          aagaaagaCATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTc                                                                                    >  1:494223/1‑191 (MQ=255)                                                                                                                                                                                                                          aagaaagaCATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGTTTATACGAACGCGCCTTAGCGGAAGGGTTCCATGTGGTGa                         >  1:89696/1‑250 (MQ=255)                                                                                                                                                                                                                            gaaagaCATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGTTTATACGAACGCGCCTTAGCGGAAGGGTTCCATGTGGTGACt                       >  1:152935/1‑250 (MQ=255)                                                                                                                                                                                                                                gaCATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTa                                                                                                                               >  1:305033/1‑142 (MQ=255)                                                                                                                                                                                                                                 aCATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGTTTATACGAACGCGCCTTAGCGGAAGGGTTCCAt                                >  1:172151/1‑236 (MQ=255)                                                                                                                                                                                                                                       aaaTTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGTTTATACGAACGCGCCTTAGCGGAAGGGTTCCATGTGGTGACTCCGAATaaaaa            <  1:577003/250‑1 (MQ=255)                                                                                                                                                                                                                                            cgcgTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGTTTATACGAACGCGCCTTAGCGGAAGGGTTCCATGTGGTGACTCCGAATAAAAAAGCCaa      >  1:650428/1‑251 (MQ=255)                                                                                                                                                                                                                                                tttGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGTTTATACGAACGCGCCTTAGCGGAAGGGTTCCATGTGGTGACTCCGAATAAAAAAGCCAATAcc  <  1:501040/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      GGCACAGGCAAACATCAGCATTAACGCCATTGCCCAAGGTTCTTCCGAACGTTCAATTTCCGCCGTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCACCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGTTTATACGAACGCGCCTTAGCGGAAGGGTTCCATGTGGTGACTCCGAATAAAAAAGCCAATACC  >  1/1463126‑1463615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑