Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,428,608	G→T	intergenic (‑10357/‑62927)	unknown ← / → unknown	SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/tRNA‑Ser‑GGA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,428,608	0	G	T	100.0%	208.4 / NA	72	intergenic (‑10357/‑62927)	unknown/unknown	SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/tRNA‑Ser‑GGA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (41/31);  total (41/31)

TGAATGAACTGGCTATCCCGTTCCGTTTAAGTCCTTCAGAACAAAAAACCGGCGAATTGACACCGCAAGACGTACAACAAATGAAAGCGTTATTTTTACAACAGCTGGCAAATTCTGCACAGTTCGACACCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCAGATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAGCGGAAATTTTGAAACAACTTGCCGATGGTCAAACCGTTGATTTTGCTTTTTTAACCCGCTTAATCGGCAGCCTGCAGGATCAACAGATTTCCATGGAAGTGTTGGTCGATAGCATTTGCAATTGGCTGGACGACGGCTGGGCGTTGTT  >  1/1428365‑1428854                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       tgaatgaaCTGGCTATCCCGTTCCGTTTAAGTCCTTCAGAACAAAAAACCGGCGAATTGACACCGCAAGACGTACAACAAATGAAAGCGTTATTTTTACAACAGCTGGCAAATTCTGCACAGTTCGACACCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGt                                                                                                                                                                                                                                                 <  1:17176/251‑1 (MQ=255)       aaCTGGCTATCCCGTTCCGTTTAAGTCCTTCAGAACAAAAAACCGGCGAATTGACACCGCAAGACGTACAACAAATGAAAGCGTTATTTTTACAACAGCTGGCAAATTCTGCACAGTTCGACACCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGac                                                                                                                                                                                                                                           <  1:854667/251‑1 (MQ=255)           ggCTATCCCGTTCCGTTTAAGTCCTTCAGAACAAAAAACCGGCGAATTGACACCGCAAGACGTACAACAAATGAAAGCGTTATTTTTACAACAGCTGGCAAATTCTGCACAGTTCGACACCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAg                                                                                                                                                                                                                                             <  1:474950/245‑1 (MQ=255)           ggCTATCCCGTTCCGTTTAAGTCCTTCAGAACAAAAAACCGGCGAATTGACACCGCAAGACGTACAACAAATGAAAGCGTTATTTTTACAACAGCTGGCAAATTCTGCACAGTTCGACACCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTAAAGacaaca                                                                                                                                                                                                                                       >  1:359330/1‑251 (MQ=255)               aTCCCGTTCCGTTTAAGTCCTTCAGAACAAAAAACCGGCGAATTGACACCGCAAGACGTACAACAAATGAAAGCGTTATTTTTACAACAGCTGGCAAATTCTGCACAGTTCGACACCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTg                                                                                                                                                                                                                                   >  1:64455/1‑251 (MQ=255)               aTCCCGTTCCGTTTAAGTCCTTCAGAACAAAAAACCGGCGAATTGACACCGCAAGACGTACAACAAATGAAAGCGTTATTTTTACAACAGCTGGCAAATTCTGCACAGTTCGACACCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTg                                                                                                                                                                                                                                   >  1:580129/1‑251 (MQ=255)               aTCCCGTTCCGTTTAAGTCCTTCAGAACAAAAAACCGGCGAATTGACACCGCAAGACGTACAACAAATGAAAGCGTTATTTTTACAACAGCTGGCAAATTCTGCACAGTTCGACACCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTg                                                                                                                                                                                                                                   >  1:437055/1‑251 (MQ=255)                        cgttTAAGTCCTTCAGAACAAAAAACCGGCGAATTGACACCGCAAGACGTACAACAAATGAAAGCGTTATTTTTACAACAGCTGGCAAATTCTGCACAGTTCGACACCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGt                                                                                                                                                                                                                          >  1:838071/1‑251 (MQ=255)                                tCCTTCAGAACAAAAAACCGGCGAATTGACACCGCAAGACGTACAACAAATGAAAGCGTTATTTTTACAACAGCTGGCAAATTCTGCACAGTTCGACACCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATAcc                                                                                                                                                                                                                   <  1:546869/250‑1 (MQ=255)                                tCCTTCAGAACAAAAAACCGGCGAATTGACACCGCAAGACGTACAACAAATGAAAGCGTTATTTTTACAACAGCTGGCAAATTCTGCACAGTTCGACACCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATAc                                                                                                                                                                                                                    <  1:26371/249‑1 (MQ=255)                                        aaCAAAAAACCGGCGAATTGACACCGCAAGACGTACAACAAATGAAAGCGTTATTTTTACAACAGCTGGCAAATTCTGCACAGTTCGACACCTTATTCCAACGAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATc                                                                                                                                                                                                            <  1:107924/249‑1 (MQ=255)                                        aaCAAAAAACCGGCGAATTGACACCGCAAGACGTACAACAAATGAAAGCGTTATTTTTACAACAGCTGGCAAATTCTGCACAGTTCGACACCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATc                                                                                                                                                                                                            <  1:13286/249‑1 (MQ=255)                                          cAAAAAACCGGCGAATTGACACCGCAAGACGTACAACAAATGAAAGCGTTATTTTTACAACAGCTGGCAAATTCTGCACAGTTCGACACCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATc                                                                                                                                                                                                            <  1:463081/247‑1 (MQ=255)                                                    gCGAATTGACACCGCAAGACGTACAACAAATGAAAGCGTTATTTTTACAACAGCTGGCAAATTCTGCACAGTTCGACACCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTAt                                                                                                                                                                                              >  1:314113/1‑251 (MQ=255)                                                               ccGCAAGACGTACAACAAATGAAAGCGTTATTTTTACAACAGCTGGCAAATTCTGCACAGTTCGACACCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGTGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATAc                                                                                                                                                                                                                    <  1:895510/218‑1 (MQ=255)                                                               ccGCAAGACGTACAACAAATGAAAGCGTTATTTTTACAACAGCTGGCAAATTCTGCACAGTTCGACACCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGa                                                                                                                                                                                   >  1:834558/1‑251 (MQ=255)                                                                      aCGTACAACAAATGAAAGCGTTATTTTTACAACAGCTGGCAAATTCTGCACAGTTCGACACCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATAcc                                                                                                                                                                                                                   <  1:759548/212‑1 (MQ=255)                                                                      aCGTACAACAAATGAAAGCGTTATTTCTACAACAGCTGGCAAATTCTGCACAGTTCGACACCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGTATTACTGGAATTCGTCGGTTTTTCCGATCCGGAAGACGCGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATgg                                                                                                                                                                               >  1:232771/1‑248 (MQ=255)                                                                          acaacaAATGAAAGCGTTATTTTTACAACAGCTGGCAAATTCTGCACAGTTCGACACCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATg                                                                                                                                                                        <  1:479584/251‑1 (MQ=255)                                                                                  tGAAAGCGTTATTTTTACAACAGCTGGCAAATTCTGCACAGTTCGACACCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGAcc                                                                                                                                                                <  1:564760/251‑1 (MQ=255)                                                                                       gCGTTATTTTTACAACAGCTGGCAAATTCTGCACAGTTCGACACCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACg                                                                                                                                                            >  1:387667/1‑250 (MQ=255)                                                                                          ttATTTTTACAACAGCTGGCAAATTCTGCACAGTTCGACACCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAg                                                                                                                                                                                                               >  1:652297/1‑196 (MQ=255)                                                                                            aTTTTTACAACAGCTGGCAAATTCTGCACAGTTCGACACCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAg                                                                                                                                                      <  1:142489/251‑1 (MQ=255)                                                                                             tttttACAACAGCTGGCAAATTCTGCACAGTTCGACACCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGacaac                                                                                                                                                                                                                                        <  1:404871/168‑1 (MQ=255)                                                                                             tttttACAACAGCTGGCAAATTCTGCACAGTTCGACACCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATAc                                                                                                                                                                                                                    <  1:102500/188‑1 (MQ=255)                                                                                             tttttACAACAGCTGGCAAATTCTGCACAGTTCGACACCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATAc                                                                                                                                                                                                                    >  1:548107/1‑188 (MQ=255)                                                                                              ttttACAACAGCTGGCAAATTCTGCACAGTTCGACACCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATAc                                                                                                                                                                                                                    <  1:231499/187‑1 (MQ=255)                                                                                                    aacaGCTGGCAAATTCTGCACAGTTCGACACCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAGCGGAAAtt                                                                                                                                              <  1:30877/251‑1 (MQ=255)                                                                                                      caGCTGGCAAATTCTGCACAGTTCGACACCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGc                                                                                                                                                           <  1:285651/236‑1 (MQ=255)                                                                                                      caGCTGGCAAATTCTGCACAGTTCGACACCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAGCGGAAAtttt                                                                                                                                            >  1:507440/1‑251 (MQ=255)                                                                                                        gCTGGCAAATTCTGCACAGTTCGACACCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTgg                                                                                                                                                                                                                           >  1:184866/1‑170 (MQ=255)                                                                                                                aTTCTGCACAGTTCGACACCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAGCGGAAATTTTGAAACAACtt                                                                                                                                  >  1:702614/1‑251 (MQ=255)                                                                                                                   cTGCACAGTTCGACACCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAGCGGAAATTTTGAAACAACTTGcc                                                                                                                               <  1:595856/251‑1 (MQ=255)                                                                                                                      cacaGTTCGACACCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAGCGGAAATTTTGAAACAACTTGCCGAt                                                                                                                            <  1:40797/251‑1 (MQ=255)                                                                                                                        caGTTCGACACCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGacaac                                                                                                                                                                                                                                        >  1:706627/1‑141 (MQ=255)                                                                                                                          gTTCGACACCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAAt                                                                                                                                                                                                                                     >  1:872102/1‑142 (MQ=255)                                                                                                                          gTTCGACACCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGAcc                                                                                                                                                                <  1:817337/211‑1 (MQ=255)                                                                                                                          gTTCGACACCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAGCGGAAATTTTGAAACAACTTGCCg                                                                                                                              <  1:719047/245‑1 (MQ=255)                                                                                                                          gTTCGACACCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAGCGGAAATTTTGAAACAACTTGCCGATGGt                                                                                                                         >  1:280142/1‑250 (MQ=255)                                                                                                                          gTTCGACACCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAGCGGAAATTTTGAAACAACTTGCCGATGGt                                                                                                                         >  1:188227/1‑250 (MQ=255)                                                                                                                               acacCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAGCGGAAATTTTGAAACAACTTGCCGATGGTCAAAc                                                                                                                    <  1:280395/250‑1 (MQ=255)                                                                                                                                cacCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAGCGGAAATTTTGAAACAACTTGCCGATGGTCAAACCg                                                                                                                  <  1:175331/251‑1 (MQ=255)                                                                                                                                 acCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAGCGGAAATTTTGAAACAACTTGCCGATGGTCAAACCGt                                                                                                                 >  1:535619/1‑251 (MQ=255)                                                                                                                                  ccTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAGCGGAAATTTTGAAACAACTTGCCGATGGTCAAACCGtt                                                                                                                >  1:31797/1‑251 (MQ=255)                                                                                                                                  ccTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAGCGGAAATTTTGAAACAACTTGCCGATGGTCAAACCGtt                                                                                                                >  1:887874/1‑251 (MQ=255)                                                                                                                                      aTTCCAATAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGGCAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAGCGGAAATTTTGAAACAACTTGCCGATGGTCAAACCGTTGAtt                                                                                                            <  1:157245/251‑1 (MQ=255)                                                                                                                                           aacaaGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAGCGGAAATTTTGAAACAACTTGCCGATGGTCAAACCGTTGATTTTGCt                                                                                                       <  1:35653/251‑1 (MQ=255)                                                                                                                                                             ccGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAGCGGAAATTTTGAAACAACTTGCCGATGGTCAAACCGTTGATTTTGCTTTTTTAACCCGCTTAATc                                                                                     <  1:596957/251‑1 (MQ=255)                                                                                                                                                             ccGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATAATACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAGCGGAAATTTTGAAACAACTTGCCGATGGTCAAACCGTTGATTTTGCTTTTTTAACCCGCTTAAt                                                                                      >  1:418221/1‑250 (MQ=255)                                                                                                                                                                   gCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAGCGGAAATTTTGAAACAACTTGCCGATGGTCAAACCGTTGATTTTGCTTTTTTAACCCGCTTAATCGGCAg                                                                                >  1:508953/1‑250 (MQ=255)                                                                                                                                                                     cGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAGCGGAAATTTTGAAACAACTTGCCGATGGTCAAACCGTTGATTTTGCTTTTTTAACCCGCTTAAt                                                                                      <  1:676777/242‑1 (MQ=255)                                                                                                                                                                            gcTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAGCGGAAATTTTGAAACAACTTGCCGATGGTCAAACCGTTGATTTTGCTTTTTTAACCCGCTTAATCGGCAGCCTGCAGGAt                                                                      >  1:33377/1‑251 (MQ=255)                                                                                                                                                                             cTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAGCGGAAATTTTGAAACAACTTGCCGATGGTCAAACCGTTGATTTTGCTTTTTTAACCCGCTTAATCGGCAGCCTGCAGGAt                                                                      <  1:438150/250‑1 (MQ=255)                                                                                                                                                                               aTGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAGCGGAAATTTTGAAACAACTTGCCGATGGTCAAACCGTTGAtt                                                                                                            >  1:173293/1‑210 (MQ=255)                                                                                                                                                                                 gAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAGCGGAAATTTTGAAACAACTTGCCGATGGTCAAACCGTTGATTTTGCTTTTTTAACCCGCTTAATCGGCAGCCTGCAGGATCAACa                                                                 >  1:673495/1‑251 (MQ=255)                                                                                                                                                                                    ttACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAGCGGAAATTTTGAAACAACTTGCCGATGGTCAAACCGTTGATTTTGCTTTTTTAACCCGCTTAATCGGCAGCCTGCAGGATCAACAGAt                                                              >  1:188880/1‑251 (MQ=255)                                                                                                                                                                                      aCTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAGCGGAAATTTTGAAACAACTTGCCGATGGTCAAACCGTTGATTTTGCTTTTTTAACCCGCTTAATCGGCAGCCTGCAGGATCAACAGAttt                                                            >  1:408491/1‑251 (MQ=255)                                                                                                                                                                                                   ggTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAGCGGAAATTTTGAAACAACTTGCCGATGGTCAAACCGTTGATTTTGCTTTTTTAACCCGCTTAATCGGCAGCCTGCAGGATCAACAGATTTCCATGGAAGTGtt                                               >  1:700040/1‑251 (MQ=255)                                                                                                                                                                                                            gATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAGCGGAAATTTTGAAACAACTTGCCGATGGTCAAAc                                                                                                                    >  1:18013/1‑173 (MQ=255)                                                                                                                                                                                                             aTCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAGCGGAAATTTTGAAACAACTTGCCGATGGTCAAACCGTTGATTTTGCTTTTTTAACCCGCTTAATCGGCAGCCTGCAGGATCAACAGATTTCCATGGAAGTGTTGGTCGATAg                                      >  1:412918/1‑250 (MQ=255)                                                                                                                                                                                                               ccGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAGCGGAAATTTTGAAACAACTTGCCGATGGTCAAACCGTTGATTTTGCTTTTTTAACCCGCTTAATCGGCAGCCTGCAGGATCAACAGATTTCCATGGAAGTGTTGGTCGATAGCAt                                   >  1:28272/1‑251 (MQ=255)                                                                                                                                                                                                                      aCGTGTTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAGCGGAAATTTTGAAACAACTTGCCGATGGTCAAACCGTTGATTTTGCTTTTTTAACCCGCTTAATCGGCAGCCTGCAGGATCAACAGATTTCCATGGAAGTGTTGGTCGATAGCATTTGCAAt                            >  1:381450/1‑251 (MQ=255)                                                                                                                                                                                                                        gtgtTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAGCGGAAATTTTGAAACAACTTGCCGATGGTCAAACCGTTGATTTTGCTTTTTTAACCCGCTTAATCGGCAGCCTGCAGGATCAACAGATTTCCATGGAAGTGTTGGTCGATAGCATTTGCAATTg                          >  1:674315/1‑251 (MQ=255)                                                                                                                                                                                                                        gtgtTGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAGCGGAAATTTTGAAACAACTTGCCGATGGTCAAACCGTTGATTTTGCTTTTTTAACCCGCTTAATCGGCAGCCTGCAGGATCAACAGATTTCCATGGAAGTGTTGGTCGATAGCATTTGCAATTg                          >  1:81750/1‑251 (MQ=255)                                                                                                                                                                                                                           ttGGAAAGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCGAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAGCGGAAATTTTGAAACAACTTGCCGATGGTCAAACCGTTGATTTTGCTTTTTTAACCCGCTTAATCGGCAGCCTGCAGGATCAACAGATTTCCATGGAAGTGTTGGTCGATAGCa                                    >  1:557990/1‑238 (MQ=255)                                                                                                                                                                                                                               aaaGCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAGCGGAAATTTTGAAACAACTTGCCGATGGTCAAACCGTTGATTTTGCTTTTTTAACCCGCTTAATCGGCAGCCTGCAGGATCAACAGATTTCCATGGAAGTGTTGGTCGATAGCATTTGCAATTGGCTGgac                   <  1:382506/251‑1 (MQ=255)                                                                                                                                                                                                                                  gCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAGCGGAAATTTTGAAACAACTTGCCGATGGTCAAACCGTTGATTTTGCTTTTTTAACCCGCTTAATCGGCAGCCTGCAGGATCAACAGATTTCCATGGAAGTGTTGGTCGATAGCATTTGCAATTGGCTGgacga                 >  1:466059/1‑250 (MQ=255)                                                                                                                                                                                                                                  gCTTTGAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAGCGGAAATTTTGAAACAACTTGCCGATGGTCAAACCGTTGATTTTGCTTTTTTAACCCGCTTAATCGGCAGCCTGCAGGATCAACAGATTTCCATGGAAGTGTTGGTCGATAGCATTTGCAATTGGCTGgacga                 >  1:873723/1‑250 (MQ=255)                                                                                                                                                                                                                                       gAACATGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAGCGGAAATTTTGAAACAACTTGCCGATGGTCAAACCGTTGATTTTGCTTTTTTAACCCGCTTAATCGGCAGCCTGCAGGAt                                                                      >  1:722576/1‑192 (MQ=255)                                                                                                                                                                                                                                            tGGCGGCATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAGCGGAAATTTTGAAACAACTTGCCGATGGTCAAACCGTTGATTTTGCTTTTTTAACCCGCTTAATCGGCAGCCTGCAGGATCAACAGATTTCCATGGAAGTGTTGGTCGATAGCATTTGCAATTGGCTGGACGACGGCTGGGCgt      >  1:266541/1‑251 (MQ=255)                                                                                                                                                                                                                                             ggcggcATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAGCGGAAATTTTGAAACAACTTGCCGATGGTCAAACCGTTGATTTTGCTTTTTTAACCCGCTTAATCGGCAGCCTGCAGGATCAACAGATTTCCATGGAAg                                                   <  1:325151/205‑1 (MQ=255)                                                                                                                                                                                                                                                ggcATATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAGCGGAAATTTTGAAACAACTTGCCGATGGTCAAACCGTTGATTTTGCTTTTTTAACCCGCTTAATCGGCAGCCTGCAGGATCAACAGATTTCCATGGAAGTGTTGGTCGATAGCATTTGCAATTGGCTGGACGACGGCTGGGCgttgtt  >  1:529734/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       TGAATGAACTGGCTATCCCGTTCCGTTTAAGTCCTTCAGAACAAAAAACCGGCGAATTGACACCGCAAGACGTACAACAAATGAAAGCGTTATTTTTACAACAGCTGGCAAATTCTGCACAGTTCGACACCTTATTCCAACAAGCGCTTGCAACAACCGTCAGCCGTCGGCGCTATGAATTACTGGAAGTCGCCGGTTTTTCCGATCCGGAAGACGTGTTGGAAAGCTTTGAACATGGCGGCAGATTACGTCAAGACAACAATTGCAAATTGGTTTATACCCAAGATCCGTTCCGAATTTATGCCAACGGCGAATGGCTGGATGAATTGACCTACGCCGAAGCGGAAATTTTGAAACAACTTGCCGATGGTCAAACCGTTGATTTTGCTTTTTTAACCCGCTTAATCGGCAGCCTGCAGGATCAACAGATTTCCATGGAAGTGTTGGTCGATAGCATTTGCAATTGGCTGGACGACGGCTGGGCGTTGTT  >  1/1428365‑1428854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑