Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,423,379	C→T	intergenic (‑5128/‑68156)	unknown ← / → unknown	SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/tRNA‑Ser‑GGA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,423,379	0	C	T	100.0%	219.5 / NA	74	intergenic (‑5128/‑68156)	unknown/unknown	SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/tRNA‑Ser‑GGA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (46/28);  total (46/28)

GTGCGGACCAATCATCGACGTATGTAAGGACTGATAGCCATTGGCTTTCGGCACGGCAATATAATCTTTTACCCGACCCGGGCGCGGTTTATAGAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTCACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGTTCGTTTTTGATGTGCTCGATCCCCAAGCGATGCGCCAACGGTGCATAAATTTCCAGGGTTTCTTTGGCGATACGACGG  >  1/1423146‑1423623                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                     gTGCGGACCAATCATCGACGTATGTAAGGACTGATAGCCATTGGCTTTCGGCACGGCAATATAATCTTTTACCCGACCCGGGCGCGGTTTATAAAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCcgcg                                                                                                                                                                                                                                     >  1:719484/1‑251 (MQ=255)                      aTGTAAGGACTGATAGCCATTGGCTTTCGGCACGGCAATATAATCTTTTACCCGACCCGGGCGCGGTTTATAAAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCa                                                                                                                                                                                                                >  1:476598/1‑251 (MQ=255)                       tGTAAGGACTGATAGCCATTGGCTTTCGGCACGGCAATATAATCTTTTACCCGACCCGGGCGCGGTTTATAAAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACAt                                                                                                                                                                                                                      <  1:700758/244‑1 (MQ=255)                       tGTAAGGACTGATAGCCATTGGCTTTCGGCACGGCAATATAATCTTTTACCCGACCCGGGCGCGGTTTATAAAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCaa                                                                                                                                                                                                               >  1:462924/1‑251 (MQ=255)                              aCTGATAGCCATTGGCTTTCGGCACGGCAATATAATCTTTTACCCGACCCGGGCGCGGTTTATAAAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGcc                                                                                                                                                                                                          >  1:99793/1‑249 (MQ=255)                               cTGATAGCCATTGGCTTTCGGCACGGCAATATAATCTTTTACCCGACCCGGGCGCGGTTTATAAAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCa                                                                                                                                                                                                                <  1:704302/242‑1 (MQ=255)                                   tAGCCATTGGCTTTCGGCACGGCAATATAATCTTTTACCCGACCCGGGCGCGGTTTATAAAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGAt                                                                                                                                                                                                   <  1:885516/251‑1 (MQ=255)                                           ggCTTTCGGCACGGCAATATAATCTTTTACCCGACCCGGGCGCGGTTTATAAAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCgg                                                                                                                                                                                           >  1:850191/1‑251 (MQ=255)                                                        gcaATATAATCTTTTACCCGACCCGGGCGCGGTTTATAAAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATAc                                                                                                                                                                                                                            >  1:121963/1‑205 (MQ=255)                                                        gcaATATAATCTTTTACCCGACCCGGGCGCGGTTTATAAAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACAt                                                                                                                                                                                                                      >  1:346955/1‑211 (MQ=255)                                                           atatAATCTTTTACCCGACCCGGGCGCGGTTTATAAAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATACTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCAt                                                                                                                                                                           >  1:435034/1‑251 (MQ=255)                                                                            acccgGGCGCGGTTTATAAAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTc                                                                                                                                                                        <  1:176756/237‑1 (MQ=255)                                                                            acccgGGCGCGGTTTATAAAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACcg                                                                                                                                                           >  1:550091/1‑250 (MQ=255)                                                                                          tataAAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACg                                                                                                                                            >  1:597052/1‑251 (MQ=255)                                                                                                ggCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACtttt                                                                                                                                      >  1:574226/1‑251 (MQ=255)                                                                                                 gCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTc                                                                                                                                                                        >  1:518560/1‑216 (MQ=255)                                                                                                 gCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTAc                                                                                                                                                             <  1:566737/227‑1 (MQ=255)                                                                                                      gCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACtt                                                                                                                                        >  1:600424/1‑243 (MQ=255)                                                                                                      gCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCaaa                                                                                                                                >  1:81370/1‑251 (MQ=255)                                                                                                      gCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTCTTGGCAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCTTTCAATCATGTCTTTGCGATTACCCCCCGCAATCTGACCGCCTTTTGGCaaa                                                                                                                                >  1:685426/1‑251 (MQ=255)                                                                                                        aTTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAAc                                                                                                                              <  1:565457/251‑1 (MQ=255)                                                                                                                  gCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAAc                                                                                                                      <  1:332959/249‑1 (MQ=255)                                                                                                                    acacGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCa                                                                                                                                                                                                                >  1:610913/1‑157 (MQ=255)                                                                                                                     cacGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATcc                                                                                                                                                                                                                    >  1:731225/1‑152 (MQ=255)                                                                                                                     cacGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTg                                                                                                                  >  1:55834/1‑250 (MQ=255)                                                                                                                     cacGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTg                                                                                                                  >  1:156753/1‑250 (MQ=255)                                                                                                                       cGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTAc                                                                                                                                                             <  1:597928/205‑1 (MQ=255)                                                                                                                       cGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTa                                                                                                                                              <  1:328831/220‑1 (MQ=255)                                                                                                                       cGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCgg                                                                                                               >  1:795504/1‑251 (MQ=255)                                                                                                                               ggTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATgg                                                                                                       >  1:129726/1‑251 (MQ=255)                                                                                                                                gTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATc                                                                                                                                                  <  1:830558/207‑1 (MQ=255)                                                                                                                                gTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATgg                                                                                                       <  1:43460/250‑1 (MQ=255)                                                                                                                                   tCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGAt                                                                                                                                                                >  1:415989/1‑190 (MQ=255)                                                                                                                                           gttCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATAc                                                                                                                                                                                                                            >  1:292577/1‑122 (MQ=255)                                                                                                                                           gttCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTAc                                                                                                                                                             <  1:20843/185‑1 (MQ=255)                                                                                                                                           gttCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCaa                                                                                           >  1:62591/1‑251 (MQ=255)                                                                                                                                             tCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGa                                                                                         >  1:16496/1‑251 (MQ=255)                                                                                                                                                   cGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTcc                                                                                   <  1:253913/251‑1 (MQ=255)                                                                                                                                                     gTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGa                                                                                                              >  1:471004/1‑222 (MQ=255)                                                                                                                                                     gTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAg                                                                                 >  1:400510/1‑251 (MQ=255)                                                                                                                                                      tGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATGCATGGCTTCAAAACCAAGATCTTCCAg                                                                                 <  1:741625/250‑1 (MQ=255)                                                                                                                                                             cgcgTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTc                                                                                                                                                                        >  1:846325/1‑156 (MQ=255)                                                                                                                                                              gcgTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATc                                                                                       >  1:110513/1‑236 (MQ=255)                                                                                                                                                              gcgTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGTTCGttttt                                                                        >  1:80084/1‑251 (MQ=255)                                                                                                                                                              gcgTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGTTCGttttt                                                                        >  1:856662/1‑251 (MQ=255)                                                                                                                                                               cgTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACa                                                                                                          >  1:56064/1‑216 (MQ=255)                                                                                                                                                               cgTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGtt                                                                               >  1:398731/1‑243 (MQ=255)                                                                                                                                                                 tAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGAc                                                                                                                                          >  1:830507/1‑182 (MQ=255)                                                                                                                                                                 tAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGTTCGTTTTTGAt                                                                     >  1:466963/1‑251 (MQ=255)                                                                                                                                                                      atCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATTCCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATc                                                                                                                                                  <  1:118447/169‑1 (MQ=255)                                                                                                                                                                        ccATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTa                                                                                                                                              <  1:662275/171‑1 (MQ=255)                                                                                                                                                                        ccATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGt                                                                                                                    <  1:853690/197‑1 (MQ=255)                                                                                                                                                                           tAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAg                                                                                 >  1:215444/1‑229 (MQ=255)                                                                                                                                                                             aTGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGTTCGTTTTTGATGTGCTCGATcc                                                          >  1:706129/1‑250 (MQ=255)                                                                                                                                                                               ggAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGTTCGTTTTTGATGTGCTCGATCCCCa                                                       >  1:257088/1‑251 (MQ=255)                                                                                                                                                                                gAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGTTCGTTTTTGATGTGCTCGATCCCCaa                                                      >  1:674996/1‑251 (MQ=255)                                                                                                                                                                                gAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGTTCGTTTTTGATGTGCTCGATCCCCaa                                                      >  1:357349/1‑251 (MQ=255)                                                                                                                                                                                gAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGC‑AACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGTTCGTTTTTGAtg                                                                    >  1:336593/1‑236 (MQ=255)                                                                                                                                                                                 aaTGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGTTCGTTTTTGATGTGCTCGATCCCCAAg                                                     <  1:48880/251‑1 (MQ=255)                                                                                                                                                                                 aaTGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGTTCGTTTTTGATGTGCTCGATCCCCAAg                                                     <  1:306005/251‑1 (MQ=255)                                                                                                                                                                                  aTGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGTTCGTTTTTGATGTGCTCGATCCCCAAg                                                     >  1:251912/1‑250 (MQ=255)                                                                                                                                                                                  aTGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGTTCGTTTTTGATGTGCTCGATCCCCAAGc                                                    <  1:322139/251‑1 (MQ=255)                                                                                                                                                                                              aTCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGTTCGTTTTTGATGTGCTCGATCCCCAAGCGATGCGCCAAc                                         <  1:416900/250‑1 (MQ=255)                                                                                                                                                                                               tCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGTTCGTTTTTGATGTGCTCGATCCCCAAGCGATGCGCCAACgg                                       <  1:409065/251‑1 (MQ=255)                                                                                                                                                                                                  tttAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGTTCGTTTTTGATGTGCTCGATCCCCAAGCGATGCGCCAACggcg                                     >  1:901830/1‑248 (MQ=255)                                                                                                                                                                                                        cgcATTTTTTGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCCTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGTTCGTTTTTGATGTGCTCGATCCCCAAGCGATGCGCCAACGGTGCATaaa                               <  1:451715/250‑1 (MQ=255)                                                                                                                                                                                                              ttttGGTAGATGGCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGTTCGTTTTTGATGTGCTCGATCCCCAAg                                                     <  1:222740/222‑1 (MQ=255)                                                                                                                                                                                                                          gCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGTTCGTTTTTGATGTGCTCGATCCCCAAGCGATGCGCCAACGGTGCATAAATTTCCAGGGTTTCTTTgg             >  1:732024/1‑250 (MQ=255)                                                                                                                                                                                                                          gCATACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGTTCGTTTTTGATGTGCTCGATCCCCAAGCGATGCGCCAACGGTGCATAAATTTCCAGGGTTTCTTTgg             >  1:224220/1‑250 (MQ=255)                                                                                                                                                                                                                            aTACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCaaaa                                                                                               <  1:726862/166‑1 (MQ=255)                                                                                                                                                                                                                            aTACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGTTCGTTTTTGAtg                                                                    <  1:636516/193‑1 (MQ=255)                                                                                                                                                                                                                             tACAAATGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGTTCGTTTTTGATGTGCTCGATCCCCAAGCGATGCGCCAACGGTGCATAAATTTCCAGGGTTTCTTTGGCGAt         >  1:524674/1‑251 (MQ=255)                                                                                                                                                                                                                                 aaTGCTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGTTCGTTTTTGATGTGCTCGATCCCCAAGCGATGCGCCAACGGTGCATAAATTTCCAGGGTTTCTTTGGCGATacga     <  1:371695/251‑1 (MQ=255)                                                                                                                                                                                                                                     cTTTTTACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGTTCGTTTTTGATGTGCTCGATCCCCAAGCGATGCGCCAACGGTGCATAAATTTCCAGGGTTTCTTTGGCGATACGACgg  <  1:559403/250‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                     GTGCGGACCAATCATCGACGTATGTAAGGACTGATAGCCATTGGCTTTCGGCACGGCAATATAATCTTTTACCCGACCCGGGCGCGGTTTATAGAGGCTGTGCATTTGTCCAAGCACACGATAGCAGGTATCCACGTTGTTCACCACGGTGCGGAACGCGTAAATATCCATAATGGAATGAAACTGCTGATCTTTTAAGCGCATTTTTTGGTAGATGGCATACAAATGCTTTTCACGCCCGAATACCCGCGCTTGAATACCCACATCCTGCAAACGCCCTTTGATTTCGTCGGAAATTCGTTCAATCATGTCTTTGCGATTACCGCGCGCAATCTGTACGACTTTTTGCAAAACCGCATAACGTTGCGGATACATGGCTTCAAAACCAAGATCTTCCAGTTCGTTTTTGATGTGCTCGATCCCCAAGCGATGCGCCAACGGTGCATAAATTTCCAGGGTTTCTTTGGCGATACGACGG  >  1/1423146‑1423623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑