Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,421,702	T→C	intergenic (‑3451/‑69833)	unknown ← / → unknown	SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/tRNA‑Ser‑GGA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,421,702	0	T	C	100.0%	184.3 / NA	64	intergenic (‑3451/‑69833)	unknown/unknown	SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/tRNA‑Ser‑GGA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/37);  total (27/37)

CAATTAATTTTCGCAAAGCGGTTTGTTTCAACCCTTCCGTAGCAGGGTAAATGGGCGTGAGGGTTTCTGCCAGAACCAGCGGTTTATTGTCATGAATAATCTGATATTCAGGATGATGAATTTCCGCCATGAAACGCCCGCGACGCACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACTGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTGGCGTACTGCTCTGCCTGCAAATCGGCAATGGGTGTGATGCGGGTGCGATCTTCATAACGGAGGGGAAGGTGAAATAACAGATCTTGCAGATTGAAGATCCCCAATTTACCTAACTTTTCCGCCACAGCGGCACCAACACCGGAAATTGCCGTTAACG  >  1/1421462‑1421935                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                          cAATTAATTTTCGCAAAGCGGTTTGTTTCAACCCTTCCGTAGCAGGGTAAATGGGCGTGAGGGTTTCTGCCAGAACCAGCGGTTTATTGTCATGAATAATCTGATATTCAGGATGATGAATTTCCGCCATGAAACGCCCGCGACGCACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGa                                                                                                                                                                                                                                 >  1:124901/1‑251 (MQ=255)                                   ttCCGTAGCAGGGTAAATGGGCGTGAGGGTTTCTGCCAGAACCAGCGGTTTATTGTCATGAATAATCTGATATTCAGGATGATGAATTTCCGCCATGAAACGCCCGCGACGCACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCa                                                                                                                                                                                               >  1:519692/1‑251 (MQ=255)                                   ttCCGTAGCAGGGTAAATGGGCGTGAGGGTTTCTGCCAGAACCAGCGGTTTATTGTCATGAATAATCTGATATTCAGGATGATGAATTTCCGCCATGAAACGCCCGCGACGCACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCa                                                                                                                                                                                               >  1:736222/1‑251 (MQ=255)                                             gggTAAATGGGCGTGAGGGTTTCTGCCAGAACCAGCGGTTTATTGTCATGAATAATCTGATATTCAGGATGATGAATTTCCGCCATGAAACGCCCGCGACGCACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTa                                                                                                                                                                                       >  1:676632/1‑249 (MQ=255)                                                   aTGGGCGTGAGGGTTTCTGCCAGAACCAGCGGTTTATTGTCATGAATAATCTGATATTCAGGATGATGAATTTCCGCCATGAAACGCCCGCGACGCACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCac                                                                                                                                                                               <  1:474931/251‑1 (MQ=255)                                                   aTGGGCGTGAGGGTTTCTGCCAGAACCAGCGGTTTATTGTCATGAATAATCTGATATTCAGGATGATGAATTTCCGCCATGAAACGCCCGCGACGCACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCac                                                                                                                                                                               <  1:874950/251‑1 (MQ=255)                                                        cGTGAGGGTTTCTGCCAGAACCAGCGGTTTATTGTCATGAATAATCTGATATTCAGGATGATGAATTTCCGCCATGAAACGCCCGCGACGCACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGc                                                                                                                                                                          <  1:363862/251‑1 (MQ=255)                                                                tttCTGCCAGAACCAGCGGTTTATTGTCATGAATAATCTGATATTCAGGATGATGAATTTCCGCCATGAAACGCCCGCGACGCACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAAt                                                                                                                                                                   <  1:676994/250‑1 (MQ=255)                                                                tttCTGCCAGAACCAGCGGTTTATTGTCATGAATAATCTGATATTCAGGATGATGAATTTCCGCCATGAAACGCCCGCGACGCACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATa                                                                                                                                                                  <  1:171748/251‑1 (MQ=255)                                                                 ttCTGCCAGAACCAGCGGTTTATTGTCATGAATAATCTGATATTCAGGATGATGAATTTCCGCCATGAAACGCCCGCGACGCACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAg                                                                                                                                                                 <  1:162016/251‑1 (MQ=255)                                                                     gCCAGAACCAGCGGTTTATTGTCATGAATAATCTGATATTCAGGATGATGAATTTCCGCCATGAAACGCCCGCGACGCACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTgg                                                                                                                                                              >  1:841185/1‑250 (MQ=255)                                                                      ccAGAACCAGCGGTTTATTGTCATGAATAATCTGATATTCAGGATGATGAATTTCCGCCATGAAACGCCCGCGACGCACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTGGCg                                                                                                                                                            <  1:212862/251‑1 (MQ=255)                                                                          aaCCAGCGGTTTATTGTCATGAATAATCTGATATTCAGGATGATGAATTTCCGCCATGAAACGCCCGCGACGCACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTGGCGTa                                                                                                                                                          >  1:776384/1‑249 (MQ=255)                                                                          aaCCAGCGGTTTATTGTCATGAATAATCTGATATTCAGGATGATGAATTTCCGCCATGAAACGCCCGCGACGCACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTGGCGTAc                                                                                                                                                         <  1:616730/250‑1 (MQ=255)                                                                                  gTTTATTGTCATGAATAATCTGATATTCAGGATGATGAATTTCCGCCATGAAACGCCCGCGACGCACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAg                                                                                                                                                                 <  1:83970/234‑1 (MQ=255)                                                                                      aTTGTCATGAATAATCTGATATTCAGGATGATGAATTTCCGCCATGAAACGCCCGCGACGCACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTGGCGTACTGCTCTGCCTGCa                                                                                                                                            >  1:45149/1‑251 (MQ=255)                                                                                         gTCATGAATAATCTGATATTCAGGATGATGAATTTCCGCCATGAAACGCCCGCGACGCACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTGGCGTACTGCTCTGCCTGCAAAt                                                                                                                                         >  1:229029/1‑251 (MQ=255)                                                                                              gAATAATCTGATATTCAGGATGATGAATTTCCGCCATGAAACGCCCGCGACGCACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAg                                                                                                                                                                 >  1:657035/1‑222 (MQ=255)                                                                                                   atCTGATATTCAGGATGATGAATTTCCGCCATGAAACGCCCGCGACGCACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAGATTAAAAAAGCGCAACGCCAATTTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTGGCGTAc                                                                                                                                                         <  1:769099/226‑1 (MQ=255)                                                                                                   atCTGATATTCAGGATGATGAATTTCCGCCATGAAACGCCCGCGACGCACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTGGCGTACTGCTCTGCCTGCAAATCGGCAATggg                                                                                                                               <  1:296495/251‑1 (MQ=255)                                                                                                     cTGATATTCAGGATGATGAATTTCCGCCATGAAACGCCCGCGACGCACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAAt                                                                                                                                                                   <  1:163689/213‑1 (MQ=255)                                                                                                          atTCAGGATGATGAATTTCCGCCATGAAACGCCCGCGACGCAGCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTGGCGTACTGCTCTGCCTGCAAATCGGCAATGGGTGTGATg                                                                                                                        >  1:304100/1‑251 (MQ=255)                                                                                                          atTCAGGATGATGAATTTCCGCCATGAAACGCCCGCGACGCACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTg                                                                                                                                                                                                 >  1:531037/1‑178 (MQ=255)                                                                                                          atTCAGGATGATGAATTTCCGCCATGAAACGCCCGCGACGCACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTGGCGTACTGCTCTGCCTGCAAATCGGCAATGGGTGTGATg                                                                                                                        >  1:409993/1‑251 (MQ=255)                                                                                                               ggATGATGAATTTCCGCCATGAAACGCCCGCGACGCACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGACTTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTGGCGTACTGCTCTGCCTGCAAATCGGCAATGGGTGTGATGCgggg                                                                                                                   >  1:440247/1‑250 (MQ=255)                                                                                                               ggATGATGAATTTCCGCCATGAAACGCCCGCGACGCACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTGGCGTACTGCTCTGCCTGCAAATCGGCAATGGGTGTGATGCGGGt                                                                                                                   >  1:230039/1‑251 (MQ=255)                                                                                                                   gatgaATTTCCGCCATGAAACGCCCGCGACGCACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTGGCGTACTGCTCTGCCTGCAAATCGGCAATGGGTGTGATGCGGGTGCGa                                                                                                               >  1:459617/1‑251 (MQ=255)                                                                                                                      gaATTTCCGCCATGAAACGCCCGCGACGCACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTGGCGTACTGCTCTGCCTGCAAATCGGCAATGGGTGTGATGCGGGTGCGAt                                                                                                              >  1:533888/1‑249 (MQ=255)                                                                                                                       aaTTTCCGCCATGAAACGCCCGCGACGCACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAg                                                                                                                                                                 >  1:258940/1‑197 (MQ=255)                                                                                                                            ccGCCATGAAACGCCCGCGACGCACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTGGCGTACTGCTCTGCCTGCAAATCGGCAATGGGTGTGATGCGGGTGCGATCTTCATaa                                                                                                      <  1:875787/251‑1 (MQ=255)                                                                                                                             cGCCATGAAACGCCCGCGACGCACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTGGCGTACTGCTCTGCCTGCAAATCGGCAATGGGTGTGATGCGGGTGCGATCTTCATAAc                                                                                                     <  1:276415/251‑1 (MQ=255)                                                                                                                             cGCCATGAAACGCCCGCGACGCACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTGGCGTACTGCTCTGCCTGCAAATCGGCAATGGGTGTGATGCGGGTGCGATCTTCATAAc                                                                                                     <  1:345114/251‑1 (MQ=255)                                                                                                                             cGCCATGAAACGCCCGCGACGCACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTGGCGTACTGCTCTGCCTGCAAATCGGCAATGGGTGTGATGCGGGTGCGATCTTCATAAc                                                                                                     <  1:492515/251‑1 (MQ=255)                                                                                                                               ccATGGAACGCCCGCGACGCACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTGGCGTACTGCTCTGCCTGCAAATCGGCAATGGGTGTGATGCGGGTg                                                                                                                  >  1:132685/1‑236 (MQ=255)                                                                                                                               ccATGAAACGCCCGCGACGCACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTGGCGTACTGCTCTGCCTGCAAATCGGCAATGGGTGTGATGCGGGTGCGATCTTCATAACgg                                                                                                   <  1:479801/251‑1 (MQ=255)                                                                                                                               ccATGAAACGCCCGCGACGCACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTGGCGTACTGCTCTGCCTGCAAATCGGCAATGGGTGTGATGCGGGTGCGATCTTCATAACgg                                                                                                   >  1:833525/1‑251 (MQ=255)                                                                                                                                     aaCGCCCGCGACGCACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTGGCGTACTGCTCTGCCTGCAAATCGGCAATGGGTGTGATGCGGGTGCGATCTTCATAACGGAGGGGa                                                                                             <  1:670105/251‑1 (MQ=255)                                                                                                                                      aCGCCCGCGACGCACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTGGCGTACTGCTCTGCCTGCAAATCGGCAATGGGTGTGATGCGGGTGCGATCTTCATAACGGAGGGGaa                                                                                            <  1:389599/251‑1 (MQ=255)                                                                                                                                           cgcgACGCACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTGGCGTACTGCTCTGCCTGCAAATCGGCAATGGGTGTGATGCGGGTGCGATCTTCATAACGGAGGGGAAGGTGa                                                                                       <  1:544619/251‑1 (MQ=255)                                                                                                                                               aCGCACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTGGCGTACTGCTCTGCCTGCAAATCGGCAATGGGTGTGATGc                                                                                                                       >  1:368471/1‑215 (MQ=255)                                                                                                                                               aCGCACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTGGCGTACTGCTCTGCCTGCAAATCGGCAATGGGTGTGATGCGGGTGCGATCTTCATAACGGAGGGGAAGGTGAAAt                                                                                    <  1:571297/250‑1 (MQ=255)                                                                                                                                                  cACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTGGCGTACTGCTCTGCCTGCAAATCGGCAATGGGTGTGATGCGGGTGCGATCTTCATAACGGAGGGGAAGGTGAAATAAc                                                                                 <  1:140841/250‑1 (MQ=255)                                                                                                                                                       cACCAAAAGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTgg                                                                                                                                                              <  1:403276/168‑1 (MQ=255)                                                                                                                                                        aCCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAAt                                                                                                                                                                   <  1:772449/162‑1 (MQ=255)                                                                                                                                                         ccAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTgg                                                                                                                                                              <  1:608281/166‑1 (MQ=255)                                                                                                                                                              ggCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACAt                                                                                                                                                                                                                   <  1:784645/108‑1 (MQ=255)                                                                                                                                                                    cACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTGGCGTACTGCTCTGCCTGCAAATCGGCAATGGGTGTGATGCGGGTGCGATCTTCATAACGGAGGGGAAGGTGAAATAACAGATCTTGCAGATTGAAGa                                                              >  1:477771/1‑251 (MQ=255)                                                                                                                                                                      cccGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAAt                                                                                                                                                                   <  1:769927/148‑1 (MQ=255)                                                                                                                                                                      cccGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTGGCGTAc                                                                                                                                                         >  1:798547/1‑158 (MQ=255)                                                                                                                                                                      cccGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTGGCGTACTGCTCTGCCTGCAAATCGGCAATGGGTGTGATGCGGGTGCGATCTTCATAACGGAGGGGAAGGTGAAATAACAGATCTTGCAGATTGAAGa                                                              <  1:593806/249‑1 (MQ=255)                                                                                                                                                                           gCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTGGCGTACTGCTCTGCCTGCAAATCGGCAATGGGTGTGATGCGGGTGCGATCTTCATAACGGAGGGGAAGGTGAAATAACAGATCTTGCAGATTGAAGATCCCCaa                                                       >  1:33258/1‑251 (MQ=255)                                                                                                                                                                              ccTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTGGCGTACTGCTCTGCCTGCAAATCGGCAATGGGTGTGATGCGGGTGCGATCTTCATAACGGAGGGGAAGGTGAAATAACAGATCTTGCAg                                                                      >  1:742415/1‑233 (MQ=255)                                                                                                                                                                                      cAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTGGCGTACTGCTCTGCCTGCAAATCGGCAATGGGTGTGATGCGGGTGCGATCTTCATAACGGAGGGGAAGGTGAAATAACAGATCTTGCAGATTGAAGATCCCCAATTTACCTAAc                                             <  1:656153/250‑1 (MQ=255)                                                                                                                                                                                              ttttCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTGGCGTACTGCTCTGCCTGCAAATCGGCAATGGGTGTGATGCGGGTGCGATCTTCATAACgg                                                                                                   >  1:544250/1‑188 (MQ=255)                                                                                                                                                                                              ttttCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTGGCGTACTGCTCTGCCTGCAAATCGGCAATGGGTGTGATGCGGGTGCGATCTTCATAACGGAGGGGAAGGTGAAATAACAGATCTTGCAGATTGAAGATCCCCAATTTACCTAACTTTTCCGcc                                    <  1:526428/251‑1 (MQ=255)                                                                                                                                                                                                ttCATTCCGGCGTTAAAAATAAAAAAGCGCAACGCCAA‑TTTGGCCGTACCGTCGGAAAGATAGGCCATCAACATCGGGCGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTGGCGTACTGCTCTGCCTGCAAATCGGCAATTGGTGTGATGCGGGTGCGATCTTCATAACGGAGGGGAAAGTGAAATAACAGATCTTGCAGATTGAAGATCCCCAATTTACCTAACTTTTCCGCcac                                  <  1:516106/251‑1 (MQ=255)                                                                                                                                                                                                                aattaaaaAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGAcc                                                                                                                                                                                                                           <  1:6617/50‑1 (MQ=255)                                                                                                                                                                                                                aattaaaaAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGAc                                                                                                                                                                                                                            <  1:344025/49‑1 (MQ=255)                                                                                                                                                                                                                   taaaaAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTGGCGTACTGCTCTGCCTGCAAATCGGCAATGGGTGTGATGCGGGTGCGATCTTCATAACGGAGGGGAAGGTGAAATAACAGATCTTGCAGATTGAAGATCCCCAATTTACCTAACTTTTCCGCCACAGCGGCACCAACACCGGaa               <  1:621920/251‑1 (MQ=255)                                                                                                                                                                                                                   taaaaAAGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTGGCGTACTGCTCTGCCTGCAAATCGGCAATGGGTGTGATGCGGGTGCGATCTTCATAACGGAGGGGAAGGTGAAATAACAGATCTTGCAGATTGAAGATCCCCAATTTACCTAACTTTTCCGCCACAGCGGCACCAACACCGGaa               <  1:523476/251‑1 (MQ=255)                                                                                                                                                                                                                     aaaaaGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTGGCGTACTGCTCTGCCTGCAAATCGGCAATGGGTGTGATGCGGGTGCGATCTTCATAACGGAGGGGAAGGTGAAATAACAGATCTTGCAGATTGAAGATCCCCAATTTACCTAACTTTTCCGCCACAGCGGCACCAACACCGGAAAt             >  1:382389/1‑251 (MQ=255)                                                                                                                                                                                                                      aaaaGCGCAACGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACGTTGCACGACGCCTTCAATAGTGGCGTACTGCTCTGCCTGCAAATCGGCAATGGGTGTGATGCGGGTGCGATCTTCATAACGGAGGGGAAGGTGAAATAACAGATCTTGCAGATTGAAGATCCCCAATTTACCTAACTTTTCCGCCACAGCGGCACCAACACCGGAAAtt            <  1:433256/251‑1 (MQ=255)                                                                                                                                                                                                                                cGCCAA‑TTTGGCGGTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTGGCGTACTGCTCTGCCTGCAAATCGGCAATGGGTGTGATGCGGGTGCGATCTTCATAACGGAGGGGAAGGTGAAATAACAGATCTTGCAGATTGAAGATCCCCAATTTACCTAACTTTTCCGCCACAGCGGCACCAACACCGGAAATTGCCGTTAACg  >  1:740073/1‑251 (MQ=255)                                                                                                                                                                                                                                              gTACCGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTGGCGTACTGCTCTGCCTGCAAATCGGCAATGGGTGTGATGCGGGTGCGATCTTCATAAc                                                                                                     <  1:266087/139‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                          CAATTAATTTTCGCAAAGCGGTTTGTTTCAACCCTTCCGTAGCAGGGTAAATGGGCGTGAGGGTTTCTGCCAGAACCAGCGGTTTATTGTCATGAATAATCTGATATTCAGGATGATGAATTTCCGCCATGAAACGCCCGCGACGCACCTCACCAAAGGCTTTCACCCGAGCACCTGGCTGCAAGCTGTTTTTCATACCGGCGTTAAAATTAAAAAAGCGCAACGCCAA‑TTTGGCGGTACTGTCGGAAAGATAGACCATCAACATCGGACGACGCCCGAATTGCACTTCGCTACTTTGCACGACGCCTTCAATAGTGGCGTACTGCTCTGCCTGCAAATCGGCAATGGGTGTGATGCGGGTGCGATCTTCATAACGGAGGGGAAGGTGAAATAACAGATCTTGCAGATTGAAGATCCCCAATTTACCTAACTTTTCCGCCACAGCGGCACCAACACCGGAAATTGCCGTTAACG  >  1/1421462‑1421935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑