Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,408,584	G→A	intergenic (‑15197/+4721)	unknown ← / ← unknown	tRNA‑Val‑GAC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,408,584	0	G	A	100.0%	89.9 / NA	32	intergenic (‑15197/+4721)	unknown/unknown	tRNA‑Val‑GAC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/19);  total (13/19)

GTTGTATATTTAGTGCCGTCGGTTTTAGTGACTAAAATTTCATTATCTTCAAATTTAGCCTGACGTACCTGATTATTTTCAACATCCGAAATGAATGTCGTATAATCCGTTATCCCTCCGCCGGAAGTGGTATTAAAACTTTGATATGCAGTCATCATGACAATGGCCACAACCACCCAAAGAATTAAATTTTTTACCATATCGTTCAAGGCATTACCTCGTATTTATAAGTTAACAACGTGTTGTAAGGTTACTATATTTATCTCAAAAATTAAAGTTATCAAATCAATTGAGCTTATTGGTAACTTAATTTGATTAGTGTTTATAACCGCTTGCAACAATATAAACTTCCCGAGATCGGTCTCGGGAGGCTTCAGGTTTACGAACTTTGACTACACTAAACAGAGAACGGATCTCTTTTAAGTATTCATCAAAACCTTCCCCCTGAAAAACTTTTACTACAAAACTTCCTTT  >  1/1408345‑1408818                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                           gttgtATATTTAGTGCCGTCGGTTTTAGTGACTAAAATTTCATTATCTTCAAATTTAGCCTGACGTACCTGATTATTTTCAACATCCGAAATGAATGTCGTATAATCCGTTATCCCTCCGCCGGAAGTGGTATTAAAACTTTGATATGCAGTCATCATGACAATGGCCACAACCACCCAAAGAATTAAATTTTTTACCATATCGTTCAAGGCATTACCTCGTATTTATAAGTTAACAACATGTTGTAAgg                                                                                                                                                                                                                                  >  1:169931/1‑250 (MQ=255) gttgtATATTTAGTGCCGTCGGTTTTAGTGACTAAAATTTCATTATCTTCAAATTTAGCCTGACGTACCTGATTATTTTCAACATCCGAAATGAATGTCGTATAATCCGTTATCCCTCCGCCGGAAGTGGTATTAAAACTTTGATATGCAGTCATCATGACAATGGCCACAACCACCCAAAGAATTAAATTTTTTACCATATCGTTCAAGGCATTACCTCGTATTTATAAGTTAACAACATGTTGTAAGGt                                                                                                                                                                                                                                 >  1:321059/1‑251 (MQ=255)    gtATATTTAGTGCCGTCGGTTTTAGTGACTAAAATTTCATTATCTTCAAATTTAGCCTGACGTACCTGATTATTTTCAACATCCGAAATGAATGTCGTATAATCCGTTATCCCTCCGCCGGAAGTGGTATTAAAACTTTGATATGCAGTCATCATGACAATGGCCACAACCACCCAAAGAATTAAATTTTTTACCATATCGTTCAAGGCATTACCTCGTATTTATAAGTTAACAACATGTTGTAAGGTTa                                                                                                                                                                                                                               >  1:41718/1‑250 (MQ=255)        atTTAGTGCCGTCGGTTTTAGTGACTAAAATTTCATTATCTTCAAATTTAGCCTGACGTACCTGATTATTTTCAACATCCGAAATGAATGTCGTATAATCCGTTATCCCTCCGCCGGAAGTGGTATTAAAACTTTGATATGCAGTCATCATGACAATGGCCACAACCACCCAAAGAATTAAATTTTTTACCATATCGTTCAAGGCATTACCTCGTATTTATAAGTTAACAACATGTTGTAAGGTTACtata                                                                                                                                                                                                                          <  1:582050/251‑1 (MQ=255)           tAGTGCCGTCGGTTTTAGTGACTAAAATTTCATTATCTTCAAATTTAGCCTGACGTACCTGATTATTTTCAACATCCGAAATGAATGTCGTATAATCCGTTATCCCTCCGCCGGAAGTGGTATTAAAACTTTGATATGCAGTCATCATGACAATGGCCACAACCACCCAAAGAATTAATTTTTTTACCATATCGTTCAAGGCATTACCTCGTATTTATAAGTTAACAACATGTTGTAAGGTTACTATAttt                                                                                                                                                                                                                       <  1:26601/251‑1 (MQ=255)              tGCCGTCGGTTTTAGTGACTAAAATTTCATTATCTTCAAATTTAGCCTGACGTACCTGATTATTTTCAACATCCGAAATGAATGTCGTATAATCCGTTATCCCTCCGCCGGAAGTGGTATTAAAACTTTGATATGCAGTCATCATGACAATGGCCACAACCACCCAAAGAATTAAATTTTTTACCATATCGTTCAAGGCATTACCTCGTATTTATAAGTTAACAACATGTTGTAAGGTTACTATATTTAtc                                                                                                                                                                                                                    <  1:477553/251‑1 (MQ=255)                              gACTAAAATTTCATTATCTTCAAATTTAGCCTGACGTACCTGATTATTTTCAACATCCGAAATGAATGTCGTATAATCCGTTATCCCTCCGCCGGAAGTGGTATTAAAACTTTGATATGCAGTCATCATGACAATGGCCACAACCACCCAAAGAATTAAATTTTTTACCATATCGTTCAAGGCATTACCTCGTATTTATAAGTTAACAACATGTTGTAAgg                                                                                                                                                                                                                                  >  1:872061/1‑221 (MQ=255)                                        tCATTATCTTCAAATTTAGCCTGACGTACCTGATTATTTTCAACATCCGAAATGAATGTCGTATAATCCGTTATCCCTCCGCCGGAAGTGGTATTAAAACTTTGATATGCAGTCATCATGACAATGGCCACAACCACCCAAAGAATTAAATTTTTTACCATATCGTTCAAGGCATTACCTCGTATTTATAAGTTAACAACATGTTGTAAGGTTACTATATTTATCTCAAAAATTAAAGTTATCAAATCAAt                                                                                                                                                                                          <  1:17795/251‑1 (MQ=255)                                                    aaTTTAGCCTGACGTACCTGATTATTTTCAACATCCGAAATGAATGTCGTATAATCCGTTATCCCTCCGCCGGAAGTGGTATTAAAACTTTGATATGCAGTCATCATGACAATGGCCACAACCACCCAAAGAATTAAATTTTTTACCATATCGTTCAAGGCATTACCTCGTATTTATAAGTTAACAACATGTTGTAAGGt                                                                                                                                                                                                                                 <  1:683583/200‑1 (MQ=255)                                                    aaTTTAGCCTGACGTACCTGATTATTTTCAACATCCGAAATGAATGTCGTATAATCCGTTATCCCTCCGCCGGAAGTGGTATTAAAACTTTGATATGCAGTCATCATGACAATGGCCACAACCACCCAAAGAATTAAATTTTTTACCATATCGTTCAAGGCATTACCTCGTATTTATAAGTTAACAACATGTTGTAAGGTTACTATATTTAtc                                                                                                                                                                                                                    >  1:422060/1‑213 (MQ=255)                                                    aaTTTAGCCTGACGTACCTGATTATTTTCAACATCCGAAATGAATGTCGTATAATCCGTTATCCCTCCGCCGGAAGTGGTATTAAAACTTTGATATGCAGTCATCATGACAATGGCCACAACCACCCAAAGAATTAAATTTTTTACCATATCGTTCAAGGCATTACCTCGTATTTATAAGTTAACAACATGTTGTAAGGTTACTATATTTATCTCAAAAATTAAAGTTATCAAATCAATTGAGCTTATTgg                                                                                                                                                                              <  1:387546/251‑1 (MQ=255)                                                         aGCCTGACGTACCTGATTATTTTCAACATCCGAAATGAATGTCGTATAATCCGTTATCCCTCCGCCGGAAGTGGTATTAAAACTTTGATATGCAGTCATCATGACAATGGCCACAACCACCCAAAGAATTAAATTTTTTACCATATCGTTCAAGGCATTACCTCGTATTTATAAGTTAACAACAtgttgt                                                                                                                                                                                                                                      >  1:103624/1‑190 (MQ=255)                                                         aGCCTGACGTACCTGATTATTTTCAACATCCGAAATGAATGTCGTATAATCCGTTATCCCTCCGCCGGAAGTGGTATTAAAACTTTGATATGCAGTCATCATGACAATGGCCACAACCACCCAAAGAATTAAATTTTTTACCATATCGTTCAAGGCATTACCTCGTATTTATAAGTTAACAACATGTTGTAAgg                                                                                                                                                                                                                                  <  1:858452/194‑1 (MQ=255)                                                             tGACGTACCTGATTATTTTCAACATCCGAAATGAATGTCGTATAATCCGTTATCCCTTCGCCGGAAGTGGTATTAAAACTTTGATATGCAGTCATCATGACAATGGCCACAACCACCCAAAGAATTAAATTTTTTACCATATCGTTCAAGGCATTACCTCGTATTTATAAGTTAACAACATGTTGTAAGGTTACTATATTTATCTCAAAAATTAAAGTTatcaaatc                                                                                                                                                                                             <  1:435483/227‑1 (MQ=255)                                                                   aCCTGATTATTTTCAACATCCGAAATGAATGTCGTATAATCCGTTATCCCTCCGCCGGAAGTGGTATTAAAACTTTGATATGCAGTCATCATGACAATGGCCACAACCACCCAAAGAATTAAATTTTTTACCATATCGTTCAAGGCATTACCTCGTATTTATAAGTTAACAACATGTTGTAAGGTTACTATATTTATCTCAAAAATTAAAGTTATCAAATCAATTGAGCTTATTGGTAACTTAATTTGAtt                                                                                                                                                               <  1:36761/251‑1 (MQ=255)                                                                                 aaCATCCGAAATGAATGTCGTATAATCCGTTATCCCTCCGCCGGAAGTGGTATTAAAACTTTGATATGCAGTCATCATGACAATGGCCACAACCACCCAAAGAATTAAATTTTTTACCATATCGTTCAAGGCATTACCTCGTATTTATAAGTTAACAACATGTTGTAAGGTTACTATATTTATCTCAAAAATTAAAGTTATCAAATCAATTGAGCTTATTGGTAACTTAATTTGATTAGTGTTTATAACCg                                                                                                                                                 <  1:461357/251‑1 (MQ=255)                                                                                   cATCCGAAATGAATGTCGTATAATCCGTTATCCCTCCGCCGGAAGTGGTATTAAAACTTTGATATGCAGTCATCATGACAATGGCCACAACCACCCAAAGAATTAAATTTTTTACCATATCGTTCAAGGCATTACCTCGTATTTATAAGTTAACAACATGTTGTAAgg                                                                                                                                                                                                                                  <  1:23211/168‑1 (MQ=255)                                                                                                 gTCGTATAATCCGTTATCCCTCCGCCGGAAGTGGTATTAAAACTTTGATATGCAGTCATCATGACAATGGCCACAACCACCCAAAGAATTAAATTTTTTACCATATCGTTCAAGGCATTACCTCGTATTTATAAGTTAACAACATGTTGTAAGGTTACTATATTTATCTCAAAAATTAAAGTTATCAAATCAATTGAGCTTATTGGTAACTTAATTTGATTAGTGTTTATAACCGCTTGCAACAATATaaa                                                                                                                                 >  1:548284/1‑251 (MQ=255)                                                                                                                       cgccgGAAGTGGTATTAAAACTTTGATATGCAGTCATCATGACAATGGCCACAACCACCCAAAGAATTAAATTTTTTACCATATCGTTCAAGGCATTACCTCGTATTTATAAGTTAACAACATGTTGTAAGGTTACTATATTTATCTCAAAAATTAAAGTTATCAAATCAATTGAGCTTATTGGTAACTTAATTTGATTAGTGTTTATAACCGCTTGCAACAATATAAACTTCCCGAGATCGGTCTCGGGa                                                                                                           <  1:249499/251‑1 (MQ=255)                                                                                                                        gccgGAAGTGGTATTAAAACTTTGATATGCAGTCATCATGACAATGGCCACAACCACCCAAAGAATTAAATTTTTTACCATATCGTTCAAGGCATTACCTCGTATTTATAAGTTAACAACATGTTGTAAGGTTACTATATTTATCTCAAAAATTAAAGTTATCAAATCAATTGAGCTTATTGGTAACTTAATTTGATTAGTGTTTATAACCGCTTGCAACAATATAAACTTCCCGAGATCGGTCTCGGGAg                                                                                                          >  1:92344/1‑251 (MQ=255)                                                                                                                                  gTATTAAAACTTTGATATGCAGTCATCATGACAATGGCCACAACCACCCAAAGAATTAAATTTTTTACCATATCGTTCAAGGCATTACCTCGTATTTATAAGTTAACAACATGTTGTAAGGTTACTATATTTATCTCAAAAATTAAAGTTATCAAATCAATTGAGCTTATTGGTAACTTAATTTGATTAGTGTTTATAACCGCTTGCAACAATATAAACTTCCCGAGATCGGtct                                                                                                                <  1:184127/235‑1 (MQ=255)                                                                                                                                  gTATTAAAACTTTGATATGCAGTCATCATGACAATGGCCACAACCACCCAAAGAATTAAATTTTTTACCATATCGTTCAAGGCATTACCTCGTATTTATAAGTTAACAACATGTTGTAAGGTTACTATATTTATCTCAAAAATTAAAGTTATCAAATCAATTGAGCTTATTGGTAACTTAATTTGATTAGTGTTTATAACCGCTTGCAACAATATAAACTTCCCGAGATCGGTCTCGGGAGGCTTCAGGtt                                                                                                >  1:35935/1‑251 (MQ=255)                                                                                                                                                         catcatGACAATGGCCACAACCACCCAAAGAATTAAATTTTTTACCATATCGTTCAAGGCATTACCTCGTATTTATAAGTTAACAACATGTTGTAAGGTTACTATATTTATCTCAAAAATTAAAGTTATCAAATCAATTGAGCTTATTGGTAACTTAATTTGATTAGTGTTTATAACCGCTTGCAACAATATAAACTTCCCGAGATCGGTCTCGGGAGGCTTCAGGTTTACGAACTTTGACTACACTAAAc                                                                         <  1:455466/251‑1 (MQ=255)                                                                                                                                                           tcatGACAATGGCCACAACCACCCAAAGAATTAAATTTTTTACCATATCGTTCAAGGCATTACCTCGTATTTATAAGTTAACAACATGTTGTAAGGTTACTATATTTATCTCAAAAATTAAAGTTATCAAATCAATTGAGCTTATTGGTAACTTAATTTGATTAGTGTTTATAACCGCTTGCAACAATATAAACTTCCCGAGATCGGTCTCGGGAGGCTTCAGGTTTACGAACTTTGACTACACTAAAc                                                                         <  1:659639/249‑1 (MQ=255)                                                                                                                                                                     ggCCACAACCACCCAAAGAATTAAATTTTTTACCATATCGTTCAAGGCATTACCTCGTATTTATAAGTTAACAACATGTTGTAAGGTTACTatat                                                                                                                                                                                                                         >  1:93219/1‑95 (MQ=255)                                                                                                                                                                     ggCCACAACCACCCAAAGAATTAAATTTTTTACCATATCGTTCAAGGCATTACCTCGTATTTATAAGTTAACAACATGTTGTAAGGTTACTATATTTATCTCAAAAATTAAAGTTATCAAATCAATTGAGCTTATTGGTAACTTAATTTGATTAGTGTTtata                                                                                                                                                     <  1:190884/163‑1 (MQ=255)                                                                                                                                                                                cccAAAGAATTAAATTTTTTACCATATCGTTCAAGGCATTACCTCGTATTTATAAGTTAACAACATGTTGTAAGGTTACTATATTTATCTCAAAAATTAAAGTTATCAAATCAATTGAGCTTATTGGTAACTTAATTTGATTAGTGTTTATAAc                                                                                                                                                   >  1:110375/1‑154 (MQ=255)                                                                                                                                                                                                            gTTCAAGGCATTACCTCGTATTTATAAGTTAACAACATGTTGTAAgg                                                                                                                                                                                                                                  <  1:667295/47‑1 (MQ=255)                                                                                                                                                                                                            gTTCAAGGCATTACCTCGTATTTATAAGTTAACAACATGTTGTAAGGTTACTATATTTATCTCAAAAATTAAAGTTATCAAATCAATTGAGCTTATTGGTAACTTAATTTGATTAGTGTTTATAACCGCTTGCAACAATATAAACTTCCCGAGATCGGTCTCGGGAGGCTTCAGGTTTACGAACTTTGACTACACTAAAc                                                                         >  1:585356/1‑200 (MQ=255)                                                                                                                                                                                                            gTTCAAGGCATTACCTCGTATTTATAAGTTAACAACATGTTGTAAGGTTACTATATTTATCTCAAAAATTAAAGTTATCAAATCAATTGAGCTTATTGGTAACTTAATTTGATTAGTGTTTATAACCGCTTGCAACAATATAAACTTCCCGAGATCGGTCTCGGGAGGCTTCAGGTTTACGAACTTTGACTACACTAAACAGAGAACGGATCTCTTTTAAGt                                                   <  1:481209/222‑1 (MQ=255)                                                                                                                                                                                                                             gTATTTATAAGTTAACAACATGTTGTAAGGTTACTATATTTATCTCAAAAATTAAAGTTATCAAATCAATTGAGCTTATTGGTAACTTAATTTGATTAGTGTTTATAACCGCTTGCAACAATATAAACTTCCCGAGATCGGTCTCGGGAGGCTTCAGGTTTACGAACTTTGACTACACTAAACAGAGAACGGATCTCTTTTAAGTATTCATCAAAACCTTCCCCCTGaaa                          >  1:230954/1‑230 (MQ=255)                                                                                                                                                                                                                                tttATAAGTTAACAACATGTTGTAAGGTTACTATATTTATCTCAAAAATTAAAGTTATCAAATCAATTGAGCTTATTGGTAACTTAATTTGATTAGTGTTTATAACCGCTTGCAACAATATAAACTTCCCGAGATCGGTCTCGGGAGGCTTCAGGTTTACGAACTTTGACTACACTAAACAGAGAACGGATCTCTTTTAAGTATTCATCAAAACCTTCCCCCTGAAAAACTTTTACTACAAAACTTCCttt  <  1:89218/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                           GTTGTATATTTAGTGCCGTCGGTTTTAGTGACTAAAATTTCATTATCTTCAAATTTAGCCTGACGTACCTGATTATTTTCAACATCCGAAATGAATGTCGTATAATCCGTTATCCCTCCGCCGGAAGTGGTATTAAAACTTTGATATGCAGTCATCATGACAATGGCCACAACCACCCAAAGAATTAAATTTTTTACCATATCGTTCAAGGCATTACCTCGTATTTATAAGTTAACAACGTGTTGTAAGGTTACTATATTTATCTCAAAAATTAAAGTTATCAAATCAATTGAGCTTATTGGTAACTTAATTTGATTAGTGTTTATAACCGCTTGCAACAATATAAACTTCCCGAGATCGGTCTCGGGAGGCTTCAGGTTTACGAACTTTGACTACACTAAACAGAGAACGGATCTCTTTTAAGTATTCATCAAAACCTTCCCCCTGAAAAACTTTTACTACAAAACTTCCTTT  >  1/1408345‑1408818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑