Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,398,091	A→G	intergenic (‑4704/+15214)	unknown ← / ← unknown	tRNA‑Val‑GAC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,398,091	0	A	G	100.0%	182.6 / NA	63	intergenic (‑4704/+15214)	unknown/unknown	tRNA‑Val‑GAC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/33);  total (30/33)

ATGTAGGGCGATCCAAACGATGAATGGGATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAACCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACACTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATA‑CGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAACGCAAAAGGTTGTTCACTCAAAAAAGCGTCTTCCTGCGGCGGCATGGTTTGCCACAGTGCCAAACGCTCCATGGTTTTCTGCAAATTTTGTAAATGTAACCGGGTTTGTTGATACATAAAAATAC  >  1/1397844‑1398337                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                        aTGTAGGGCGATCCAAACGATGAATGGGATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCtt                                                                                                                                                                                                                                                      >  1:168600/1‑251 (MQ=255) aTGTAGGGCGATCCAAACGATGAATGGGATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCtt                                                                                                                                                                                                                                                      >  1:371798/1‑251 (MQ=255)           aTCCAAACGATGAATGGGATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATa                                                                                                                                                                                                                                            <  1:892619/251‑1 (MQ=255)           aTCCAAACGATGAATGGGATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATa                                                                                                                                                                                                                                            <  1:441808/251‑1 (MQ=255)                aaCGATGAATGGGATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGAc                                                                                                                                                                                                                                       <  1:387801/251‑1 (MQ=255)                  cGATGAATGGGATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGa                                                                                                                                                                                                                                        >  1:557692/1‑248 (MQ=255)                      gAATGGGATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATgg                                                                                                                                                                                                                                 >  1:741930/1‑251 (MQ=255)                       aaTGGGATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATgg                                                                                                                                                                                                                                 <  1:506046/250‑1 (MQ=255)                           ggATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAAtt                                                                                                                                                                                                                            >  1:529538/1‑251 (MQ=255)                           ggATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAAtt                                                                                                                                                                                                                            >  1:510830/1‑251 (MQ=255)                                                      cgcAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTc                                                                                                                                                                                                  >  1:419478/1‑250 (MQ=255)                                                         aaGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCcaac                                                                                                                                                                                              <  1:648555/251‑1 (MQ=255)                                                                    cacaAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCCCCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTcc                                                                                                                                                                                                 <  1:553562/237‑1 (MQ=255)                                                                     acaAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATa                                                                                                                                                                                  <  1:673244/251‑1 (MQ=255)                                                                     acaAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATa                                                                                                                                                                                  <  1:181608/251‑1 (MQ=255)                                                                        aaTTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATA‑cgc                                                                                                                                                                              <  1:856226/251‑1 (MQ=255)                                                                          ttGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATA‑CGCgg                                                                                                                                                                            <  1:616763/251‑1 (MQ=255)                                                                                  cATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATA‑CGCGGAATaaaaa                                                                                                                                                                    <  1:157613/251‑1 (MQ=255)                                                                                        gATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTAAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATA‑cgc                                                                                                                                                                              >  1:197741/1‑235 (MQ=255)                                                                                           cAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATAc                                                                                                                                                                                                                                           <  1:710397/172‑1 (MQ=255)                                                                                                     gCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATA‑CGCGGAATAAAAAt                                                                                                                                                                   >  1:754942/1‑233 (MQ=255)                                                                                                               aaCATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACCTA‑CGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTc                                                                                                                                          >  1:343761/1‑248 (MQ=255)                                                                                                                  aTCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATA‑CGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTa                                                                                                                                    <  1:756307/251‑1 (MQ=255)                                                                                                                         ccgGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTCCATA‑CGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAAc                                                                                                                              <  1:65753/250‑1 (MQ=255)                                                                                                                           ggCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGAc                                                                                                                                                                                                                                       <  1:830589/144‑1 (MQ=255)                                                                                                                           ggCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATg                                                                                                                                                                                          >  1:331137/1‑189 (MQ=255)                                                                                                                                aTTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATA‑CGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAACGCAAAAgg                                                                                                                      <  1:254480/251‑1 (MQ=255)                                                                                                                                     ccGCAGCCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATTGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATA‑CGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAACGCAAAAg                                                                                                                       <  1:429152/245‑1 (MQ=255)                                                                                                                                           ccAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATgg                                                                                                                                                                                                                                 >  1:633386/1‑134 (MQ=255)                                                                                                                                           ccAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATA‑CGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAACGCAAAAGGTTGTTCACTCa                                                                                                           >  1:842074/1‑251 (MQ=255)                                                                                                                                           ccAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATA‑CGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAACGCAAAAGGTTGTTCACTCa                                                                                                           >  1:522043/1‑251 (MQ=255)                                                                                                                                              gTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATA‑CGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAACGCAAAAGGTTGTTCACTCaaaa                                                                                                        >  1:641749/1‑251 (MQ=255)                                                                                                                                              gTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATA‑CGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAACGCAAAAGGTTGTTCACTCaaaa                                                                                                        >  1:709783/1‑251 (MQ=255)                                                                                                                                                aCATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATA‑CGCGGAATaaaaa                                                                                                                                                                    >  1:594255/1‑189 (MQ=255)                                                                                                                                                aCATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATA‑CGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATgg                                                                                                                                      >  1:139666/1‑219 (MQ=255)                                                                                                                                                aCATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATA‑CGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAACGCAAAAGGTTGTTCACTCaaaaaa                                                                                                      >  1:572707/1‑251 (MQ=255)                                                                                                                                                     atcCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATAc                                                                                                                                                                                                                                           <  1:57975/114‑1 (MQ=255)                                                                                                                                                           aTACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATA‑CGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAACGCAAAAGGTTGTTCACTCAAAAAAGCGTCTTc                                                                                              >  1:235723/1‑248 (MQ=255)                                                                                                                                                               aaaaTCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATA‑CGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAACGCAAAAGGTTGTTCACTCAAAAAAGCGTCTTCCTgcggc                                                                                       <  1:14741/251‑1 (MQ=255)                                                                                                                                                                aaatcGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATA‑CGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAACGCAAAAGGTTGTTCACTCAAAAAAGCGTCTTCCTgcggcg                                                                                      >  1:655340/5‑251 (MQ=255)                                                                                                                                                                         aaTTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATA‑CGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAACGCAAAAGGTTGTTCACTCAAAAAAGCGTCTTCCTGCGGCGGCATGGttt                                                                             <  1:621874/251‑1 (MQ=255)                                                                                                                                                                          aTTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATA‑CGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAACGCAAAAGGTTGTTCACTCAAAAAAGCGTCTTCCTGCGGCGGCATGGTTTg                                                                            >  1:403692/1‑251 (MQ=255)                                                                                                                                                                           ttCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATA‑CGCGGAATAAAAAGCCATTGCAGCCATTCCGTCGGACTCATGGTATCC‑ACGCAAAAGGTTGTTCACTCAAAAAAGCGTCTTCCTGCGGCGGCATGGTTTGcc                                                                          <  1:409321/251‑1 (MQ=255)                                                                                                                                                                                tgttgttgCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATA‑CGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAACGCAAAAGGTTGTTCACTCAAAAAAGCGTCTTCCTGCGGCGGCATGGTTTGCCACAg                                                                      <  1:559155/251‑1 (MQ=255)                                                                                                                                                                                  ttgttgCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATA‑CGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAACGCAAAAGGTTGTTCACTCAAAAAAGCGTCTTCCTGCGGCGGCATGGTTTGCcac                                                                        >  1:219528/1‑247 (MQ=255)                                                                                                                                                                                           caACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATA‑CGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAACGCAAAAGGTTGTTCACTCAAAAAAGCGTCTTCCTGCGGCGGCATGGTTTGCCACAGTGCCAAAc                                                              <  1:55316/248‑1 (MQ=255)                                                                                                                                                                                           caACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATA‑CGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAACGCAAAAGGTTGTTCACTCAAAAAAGCGTCTTCCTGCGGCGGCATGGTTTGCCACAGTGCCAAACGc                                                            <  1:528142/250‑1 (MQ=255)                                                                                                                                                                                                       ttCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATAc                                                                                                                                                                                                                                           <  1:881780/64‑1 (MQ=255)                                                                                                                                                                                                                 gggAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATA‑CGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAACGCAAAAGGTTGTTCACTCAAAAAAGCGTCTTCCTGCGGCGGCATGGTTTGCCACAGTGCCAAACGCTCCATGGTTTTCTGCAAATTTTg                                     >  1:684354/1‑251 (MQ=255)                                                                                                                                                                                                                      cAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCa                                                                                                                                                                                                                               <  1:460468/61‑1 (MQ=255)                                                                                                                                                                                                                      cAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAGCGGCGCTTGGCTTTCCAACAATGCGTACATA‑CGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAACGCAAAAGGTTGTTCACTCAAAAAAGCGTCTTCCTGCGGCGGCATGGTTTGCCACAGTGCCAAAc                                                              <  1:24369/221‑1 (MQ=255)                                                                                                                                                                                                                            cgcagCCAATTAACCACCTTCCTTAAACGCTTCCGCAAAATGCGGACGGATGGCAATTTGGTTCGCCAACGGCGCTTGGCTTTCCAACAATGCGTAC‑TACCGCGGAATAAAAATCCATAGGACCAATTCGGTGGGATTCAGGGTATCCAACGCAAAAGTTTGTTCCCTCAAAAAGGGTTTTTCCTGCGGCGGAATGGTTTGCCAAGGTGCCAAACGCT‑CATGGTTTTCTGCAAATTTTGTAAATGTAACCg                         <  1:294351/247‑1 (MQ=255)                                                                                                                                                                                                                                   aaaTAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATA‑CGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAACGCAAAAGGTTGTTCACTCAAAAAAGCGTCTTCCTGCGGCGGCATGGTTTGCCACAGTGCCAAACGCTCCATGGTTTTCTGCAAATTTTGTAAATGTAACCGGGTttg                   >  1:755935/1‑251 (MQ=255)                                                                                                                                                                                                                                    aaTAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATA‑CGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAACGCAAAAGGTTGTTCACTCAAAAAAGCGTCTTCCTGCGGCGGCATGGTTTGCCACAGTGCCAAACGCTCCATGGttt                                                  >  1:818575/1‑219 (MQ=255)                                                                                                                                                                                                                                      tAACCATCTTCCTATAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATA‑CGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAACGCAAAAGGTTGTTCACTCAAAAAAGCGTCTTCCTGCGGCGGCATGGTTTGCCACAGTGCCAAACGCTCCATGGTTTTCTGCAAATTTTGTAAATGTAACCGGGTttgttg                <  1:668896/251‑1 (MQ=255)                                                                                                                                                                                                                                         ccATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATA‑CGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAACGCAAAAGGTTGTTCACTCAAAAAAGCGTCTTCCTGCGGCGGCATGGTTTGCCACAGTGCCAAACGCTCCATGGTTTTCTGCAAATTTTGTAAATGTAACCGGGTTTGTTGATa             >  1:33885/1‑251 (MQ=255)                                                                                                                                                                                                                                               tCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATA‑CGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATcc                                                                                                                                 >  1:442715/1‑129 (MQ=255)                                                                                                                                                                                                                                               tCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATA‑CGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAACGCAAAAGGTTGTTCACTCAAAAAAGCGTCTTCCTGCGGCGGCATGGTTTGCCACAGTGCCAAACGCTCCATGGTTTTCTGCAAATTTTGTAAATGTAACCGGGTTTGTTGATACATaaa       >  1:382598/1‑251 (MQ=255)                                                                                                                                                                                                                                                ccTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATA‑CGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAACGCAAAAGGTTGTTCACTCAAAAAAGCGTCTTCCTGCGGCGGCATGGTTTGCCACAGTGCCAAACGCTCCATGGTTTTCTGCAAATTTTGTAAATGTAACCGGGTTTGTTGa               >  1:82797/1‑242 (MQ=255)                                                                                                                                                                                                                                                  tttAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATA‑CGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAACGCAAAAGGTTGTTCACTCAAAAAAGCGTCTTCCTGCGGCGGCATGGTTTGCCACAGTGCCAAACGCTCCATGGTTTTCTGCAAATTTTGTAAATGTAACCGGGTTTGTTGATACATAAAAAt    <  1:674780/251‑1 (MQ=255)                                                                                                                                                                                                                                                    tAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATA‑CGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAACGCAAAAGGTTGTTCACTCAAAAAAGCGTCTTCCTGCGGCGGCATGGTTTGCCACAGTGCCAAACGCTCCATGGTTTTCTGCAAATTTTGTAAATGTAACCGGGTTTGTTGATACATAAAAATAc  <  1:273478/251‑1 (MQ=255)                                                                                                                                                                                                                                                    tAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATA‑CGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAACGCAAAAGGTTGTTCACTCAAAAAAGCGTCTTCCTGCGGCGGCATGGTTTGCCACAGTGCCAAACGCTCCATGGTTTTCTGCAAATTTTGTAAATGTAACCGGGTTTGTTGATACATAAAAATAc  <  1:531473/251‑1 (MQ=255)                                                                                                                                                                                                                                                    tAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATA‑CGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAACGCAAAAGGTTGTTCACTCAAAAAAGCGTCTTCCTGCGGCGGCATGGTTTGCCACAGTGCCAAACGCTCCATGGTTTTCTGCAAATTTTGTAAATGTAACCGGGTTTGTTGATACATAAAAATAc  <  1:132969/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                        ATGTAGGGCGATCCAAACGATGAATGGGATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAACCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACACTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATA‑CGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAACGCAAAAGGTTGTTCACTCAAAAAAGCGTCTTCCTGCGGCGGCATGGTTTGCCACAGTGCCAAACGCTCCATGGTTTTCTGCAAATTTTGTAAATGTAACCGGGTTTGTTGATACATAAAAATAC  >  1/1397844‑1398337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑