Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,398,006	C→T	intergenic (‑4619/+15299)	unknown ← / ← unknown	tRNA‑Val‑GAC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,398,006	0	C	T	100.0%	198.4 / NA	67	intergenic (‑4619/+15299)	unknown/unknown	tRNA‑Val‑GAC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/33);  total (34/33)

TTTGTTTTTGCTCAAATTGGTGACATACCAAATTTGCCGTTTCGCTGTTCAACGCAAACAACAATACGCCTGATGTAGGGCGATCCAAACGATGAATGGGATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAACCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACACTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATACGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAACGCAAAAGGTTGTTCACTCAAAAAAGCGTCTTCCTGCGGC  >  1/1397772‑1398252                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                       tttgtttTTGCTCAAATTGGTGACATACCAAATTTGCCGTTTCGCTGTTCAACGCAAACAACAATACGCCTGATGTAGGGCGATCCAAACGATGAATGGGATACACCAACTGCCCGATTTGATCGCGAAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCAttgt                                                                                                                                                                                                                                         <  1:352832/250‑1 (MQ=255)               aaTTGGTGACATACCAAATTTGCCGTTTCGCTGTTCAACGCAAACAACAATACGCCTGATGTAGGGCGATCCAAACGATGAATGGGATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACttt                                                                                                                                                                                                                          >  1:638170/1‑251 (MQ=255)                                     ccGTTTCGCTGTTCAACGCAAACAACAATACGCCTGATGTAGGGCGATCCAAACGATGAATGGGATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGc                                                                                                                                                                                                     <  1:159186/250‑1 (MQ=255)                                        tttCGCTGTTCAACGCAAACAACAATACGCCTGATGTAGGGCGATCCAAACGATGAATGGGATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATa                                                                                                                                                                                                 <  1:534616/251‑1 (MQ=255)                                             cTGTTCAACGCAAACAACAATACGCCTGATGTAGGGCGATCCAAACGATGAATGGGATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTc                                                                                                                                                                                             <  1:349528/250‑1 (MQ=255)                                             cTGTTCAACGCAAACAACAATACGCCTGATGTAGGGCGATCCAAACGATGAATGGGATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTCGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTc                                                                                                                                                                                             <  1:827663/250‑1 (MQ=255)                                                ttCAACGCAAACAACAATACGCCTGATGTAGGGCGATCCAAACGATGAATGGGATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGcc                                                                                                                                                                                         <  1:799087/251‑1 (MQ=255)                                                   aaCGCAAACAACAATACGCCTGATGTAGGGCGATCCAAACGATGAATGGGATACCCCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACAT‑CCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAAt                                                                                                                                                                                     <  1:271617/251‑1 (MQ=255)                                                      gCAAACAACAATACGCCTGATGTAGGGCGATCCAAACGATGAATGGGATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATaa                                                                                                                                                                                   >  1:308587/1‑251 (MQ=255)                                                         aacaacAATACGCCTGATGTAGGGCGATCCAAACGATGAATGGGATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCa                                                                                                                                                                                <  1:296321/251‑1 (MQ=255)                                                           caacaaTACGCCTGATGTAGGGCGATCCAAACGATGAATGGGATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCAt                                                                                                                                                                               >  1:662161/1‑250 (MQ=255)                                                             acaaTACGCCTGATGTAGGGCGATCCAAACGATGAATGGGATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCtt                                                                                                                                                                            >  1:666749/1‑251 (MQ=255)                                                             acaaTACGCCTGATGTAGGGCGATCCAAACGATGAATGGGATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCtt                                                                                                                                                                            >  1:429333/1‑251 (MQ=255)                                                              caaTACGCCTGATGTAGGGCGATCCAAACGATGAATGGGATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCtt                                                                                                                                                                            >  1:732270/1‑250 (MQ=255)                                                                    gCCTGATGTAGGGCGATCCAAACGATGAATGGGATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCaa                                                                                                                                                                                       >  1:195942/1‑233 (MQ=255)                                                                     ccTGATGTAGGGCGATCCAAACGATGAATGGGATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAac                                                                                                                                                                    <  1:300664/251‑1 (MQ=255)                                                                      cTGATGTAGGGCGATCCAAACGATGAATGGGATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAacg                                                                                                                                                                   <  1:112201/251‑2 (MQ=255)                                                                        gATGTAGGGCGATCCAAACGATGAATGGGATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATa                                                                                                                                                                                                 >  1:537079/1‑219 (MQ=255)                                                                        gATGTAGGGCGATCCAAACGATGAATGGGATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAacg                                                                                                                                                                   >  1:288581/1‑248 (MQ=255)                                                                        gATGTAGGGCGATCCAAACGATGAATGGGATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAacg                                                                                                                                                                   >  1:709898/1‑248 (MQ=255)                                                                         aTGTAGGGCGATCCAAACGATGAATGGGATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCtt                                                                                                                                                                >  1:168600/1‑251 (MQ=255)                                                                         aTGTAGGGCGATCCAAACGATGAATGGGATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCtt                                                                                                                                                                >  1:371798/1‑251 (MQ=255)                                                                                   aTCCAAACGATGAATGGGATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCa                                                                                                                                                                                >  1:786046/1‑225 (MQ=255)                                                                                   aTCCAAACGATGAATGGGATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATa                                                                                                                                                      <  1:441808/251‑1 (MQ=255)                                                                                   aTCCAAACGATGAATGGGATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATa                                                                                                                                                      <  1:892619/251‑1 (MQ=255)                                                                                        aaCGATGAATGGGATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACtt                                                                                                                                                                                                                           >  1:771317/1‑177 (MQ=255)                                                                                        aaCGATGAATGGGATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGAc                                                                                                                                                 <  1:387801/251‑1 (MQ=255)                                                                                          cGATGAATGGGATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGa                                                                                                                                                  >  1:557692/1‑248 (MQ=255)                                                                                              gAATGGGATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATgg                                                                                                                                           >  1:741930/1‑251 (MQ=255)                                                                                               aaTGGGATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATgg                                                                                                                                           <  1:506046/250‑1 (MQ=255)                                                                                                  gggATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCAttgttg                                                                                                                                                                                                                                       >  1:306089/1‑155 (MQ=255)                                                                                                   ggATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCAttgt                                                                                                                                                                                                                                         <  1:674519/152‑1 (MQ=255)                                                                                                   ggATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCAttgt                                                                                                                                                                                                                                         >  1:285526/1‑152 (MQ=255)                                                                                                   ggATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTc                                                                                                                                                                                             <  1:234208/196‑1 (MQ=255)                                                                                                   ggATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAacgc                                                                                                                                                                  <  1:319320/223‑3 (MQ=255)                                                                                                   ggATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAAtt                                                                                                                                      >  1:529538/1‑251 (MQ=255)                                                                                                   ggATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAAtt                                                                                                                                      >  1:510830/1‑251 (MQ=255)                                                                                                                              cgcAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTc                                                                                                            >  1:419478/1‑250 (MQ=255)                                                                                                                                 aaGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCcaac                                                                                                        <  1:648555/251‑1 (MQ=255)                                                                                                                                            cacaAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCCCCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTcc                                                                                                           <  1:553562/237‑1 (MQ=255)                                                                                                                                             acaAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATa                                                                                            <  1:673244/251‑1 (MQ=255)                                                                                                                                             acaAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATa                                                                                            <  1:181608/251‑1 (MQ=255)                                                                                                                                                aaTTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATAcgc                                                                                         <  1:856226/251‑1 (MQ=255)                                                                                                                                                  ttGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATACGCgg                                                                                       <  1:616763/251‑1 (MQ=255)                                                                                                                                                          cATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATACGCGGAATaaaaa                                                                               <  1:157613/251‑1 (MQ=255)                                                                                                                                                                gATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTAAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATAcgc                                                                                         >  1:197741/1‑235 (MQ=255)                                                                                                                                                                   cAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATAc                                                                                                                                                     <  1:710397/172‑1 (MQ=255)                                                                                                                                                                             gCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATACGCGGAATAAAAAt                                                                              >  1:754942/1‑233 (MQ=255)                                                                                                                                                                             gCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCCGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCa                                                                                                                                                                                <  1:420761/135‑1 (MQ=255)                                                                                                                                                                                       aaCATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACCTACGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTc                                                     >  1:343761/1‑248 (MQ=255)                                                                                                                                                                                          aTCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATACGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTa                                               <  1:756307/251‑1 (MQ=255)                                                                                                                                                                                                 ccgGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTCCATACGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAAc                                         <  1:65753/250‑1 (MQ=255)                                                                                                                                                                                                   ggCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGAc                                                                                                                                                 <  1:830589/144‑1 (MQ=255)                                                                                                                                                                                                   ggCTTATTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATg                                                                                                    >  1:331137/1‑189 (MQ=255)                                                                                                                                                                                                        aTTCACCGCAACCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATACGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAACGCAAAAgg                                 <  1:254480/251‑1 (MQ=255)                                                                                                                                                                                                             ccGCAGCCAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATTGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATACGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAACGCAAAAg                                  <  1:429152/245‑1 (MQ=255)                                                                                                                                                                                                                   ccAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATgg                                                                                                                                           >  1:633386/1‑134 (MQ=255)                                                                                                                                                                                                                   ccAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATACGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAACGCAAAAGGTTGTTCACTCa                      >  1:842074/1‑251 (MQ=255)                                                                                                                                                                                                                   ccAGTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATACGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAACGCAAAAGGTTGTTCACTCa                      >  1:522043/1‑251 (MQ=255)                                                                                                                                                                                                                      gTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATACGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAACGCAAAAGGTTGTTCACTCaaaa                   >  1:709783/1‑251 (MQ=255)                                                                                                                                                                                                                      gTACATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATACGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAACGCAAAAGGTTGTTCACTCaaaa                   >  1:641749/1‑251 (MQ=255)                                                                                                                                                                                                                        aCATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATACGCGGAATaaaaa                                                                               >  1:594255/1‑189 (MQ=255)                                                                                                                                                                                                                        aCATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATACGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATgg                                                 >  1:139666/1‑219 (MQ=255)                                                                                                                                                                                                                        aCATCATCCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATACGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAACGCAAAAGGTTGTTCACTCaaaaaa                 >  1:572707/1‑251 (MQ=255)                                                                                                                                                                                                                             atcCTGATACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATAc                                                                                                                                                     <  1:57975/114‑1 (MQ=255)                                                                                                                                                                                                                                   aTACAAAATCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATACGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAACGCAAAAGGTTGTTCACTCAAAAAAGCGTCTTc         >  1:235723/1‑248 (MQ=255)                                                                                                                                                                                                                                       aaaaTCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACGCTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATACGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAACGCAAAAGGTTGTTCACTCAAAAAAGCGTCTTCCTgcggc  <  1:14741/251‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                       TTTGTTTTTGCTCAAATTGGTGACATACCAAATTTGCCGTTTCGCTGTTCAACGCAAACAACAATACGCCTGATGTAGGGCGATCCAAACGATGAATGGGATACACCAACTGCCCGATTTGATCGCGCAAGATTTGCATCACAAATTGTGTTTCATGGCGATCAAGCCAACTGCGATGCACCAACATCCCCGCCGGCTTATTCACCGCAACCAGTACATCATCCTGATACAAAACCGCTAAATTCATTGTTGTTGCAGCAACTTTTCGATTTCAATGATCGGGAGCAATAACTCCGCCAAATAACCATCTTCCTTTAACACTTCCTCTAAATACGGACTGATGGCAATTTGGCTCGGCAACGGCGCTTGGCTTTCCAACAATGCGTACATACGCGGAATAAAAATCCATTGCAGCCATTCCGTCGGACTCATGGTATCCAACGCAAAAGGTTGTTCACTCAAAAAAGCGTCTTCCTGCGGC  >  1/1397772‑1398252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑