Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,396,169	A→G	intergenic (‑2782/+17136)	unknown ← / ← unknown	tRNA‑Val‑GAC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,396,169	0	A	G	100.0%	159.4 / NA	56	intergenic (‑2782/+17136)	unknown/unknown	tRNA‑Val‑GAC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/28);  total (28/28)

CTGAGGTTGTGAGGGAATTTCAGTCACGGCTAATTCCGGACGTAACGCCTGCCAAGGCAATGTGTTATTAGATAAAGAAGATAAAATCACGTTAATCTCTGGGTTATATTTAATTTGCGCGCATTATCGCATATTTCTTCGCGTCTTTATTAATCTCGCGTAAAAATTTGCTAGAAAATCCATGATAAAAAGCGTATAGTGACACAGTTACCACGTCACTAAAACGGTTTAGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCAGAAG  >  1/1395939‑1396407                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               cTGAGGTTGTGAGGGAATTTCAGTCACGGCTAATTCCGGACGTAACGCCTGCCAAGGCAATGTGTTATTAGATAAAGAAGATAAAATCACGTTAATCTCTGGGTTATATTTAATTTGCGCGCATTATCGCATATTTCTTCGCGTCTTTATTAATCTCGCGTAAAAATTTGCTAGAAAATCCATGATAAAAAGCGTATAGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAg                                                                                                                                                                                                                            <  1:813402/251‑1 (MQ=255)                         cACGGCTAATTCCGGACGTAACGCCTGCCAAGGCAATGTGTTATTAGATAAAGAAGATAAAATCACGTTAATCTCTGGGTTATATTTAATTTGCGCGCATTATCGCATATTTCTTCGCGTCTTTATTAATCTCGCGTAAAAATTTGCTAGAAAATCCATGATAAAAAGCGTATAGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTgg                                                                                                                                                                                                    <  1:422443/251‑1 (MQ=255)                               tAATTCCGGACGTAACGCCTGCCAAGGCAATGTGTTATTAGATAAAGAAGATAAAATCACGTTAATCTCTGGGTTATATTTAATTTGCGCGCATTATCGCATATTTCTTCGCGTCTTTATTAATCTCGCGTAAAAATTTGCTAGAAAATCCATGATAAAAAGCGTATAGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATgg                                                                                                                                                                                              >  1:193692/1‑251 (MQ=255)                                 aTTCCGGACGTAACGCCTGCCAAGGCAATGTGTTATTAGATAAAGAAGATAAAATCACGTTAATCTCTGGGTTATATTTAATTTGCGCGCATTATCGCATATTTCTTCGCGTCTTTATTAATCTCGCGTAAAAATTTGCTAGAAAATCCATGATAAAAAGCGTATAGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGc                                                                                                                                                                                            <  1:227438/251‑1 (MQ=255)                                            aaCGCCTGCCAAGGCAATGTGTTATTAGATAAAGAAGATAAAATCACGTTAATCTCTGGGTTATATTTAATTTGCGCGCATTATCGCATATTTCTTCGCGTCTTTATTAATCTCGCGTAAAAATTTGCTAGAAAATCCATGATAAAAAGCGTATAGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGa                                                                                                                                                                                 <  1:626701/251‑1 (MQ=255)                                                  tGCCAAGGCAATGTGTTATTAGATAAAGAAGATAAAATCACGTTAATCTCTGGGTTATATTTAATTTGCGCGCATTATCGCATATTTCTTCGCGTCTTTATTAATCTCGCGTAAAAATTTGCTAGAAAATCCATGATAAAAAGCGTATAGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTAttt                                                                                                                                                                                      <  1:192096/240‑1 (MQ=255)                                                  tGCCAAGGCAATGTGTTATTAGATAAAGAAGATAAAATCACGTTAATCTCTGGGTTATATTTAATTTGCGCGCATTATCGCATATTTCTTCGCGTCTTTATTAATCTCGCGTAAAAATTTGCTAGAAAATCCATGATAAAAAGCGTATAGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAg                                                                                                                                                                           >  1:173866/1‑251 (MQ=255)                                                  tGCCAAGGCAATGTGTTATTAGATAAAGAAGATAAAATCACGTTAATCTCTGGGTTATATTTAATTTGCGCGCATTATCGCATATTTCTTCGCGTCTTTATTAATCTCGCGTAAAAATTTGCTAGAAAATCCATGATAAAAAGCGTATAGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAg                                                                                                                                                                           <  1:478614/251‑1 (MQ=255)                                                                 ttattaGATAAAGAAGATAAAATCACGTTAATCTCTGGGTTATATTTAATTTGCGCGCATTATCGCATATTTCTTCGCGTCTTTATTAATCTCGCGTAAAAATTTGCTAGAAAATCCATGATAAAAAGCGTATAGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGCCCGTGAAGGCGaaa                                                                                                                                                            >  1:269021/1‑251 (MQ=255)                                                                          aaaGAAGATAAAATCACGTTAATCTCTGGGTTATATTTAATTTGCGCGCATTATCGCATATTTCTTCGCGTCTTTATTAATCTCGCGTAAAAATTTGCTAGAAAATCCATGATAAAAAGCGTATAGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGCCCGTGAAGGCGAAAACGTTAccc                                                                                                                                                   <  1:503998/251‑1 (MQ=255)                                                                          aaaGAAGATAAAATCACGTTAATCTCTGGGTTATATTTAATTTGCGCGCATTATCGCATATTTCTTCGCGTCTTTATTAATCTCGCGTAAAAATTTGCTAGAAAATCCATGATAAAAAGCGTATAGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGCCCGTGAAGGCGAAAACGTTAccc                                                                                                                                                   <  1:680993/251‑1 (MQ=255)                                                                                gaTAAAATCACGTTAATCTCTGGGTTATATTTAATTTGCGCGCATTATCGCATATTTCTTCGCGTCTTTATTAATCTCGCGTAAAAATTTGCTAGAAAATCCATGATAAAAAGCGTATAGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAAt                                                                                                                                                                                                <  1:730014/200‑1 (MQ=255)                                                                                 aTAAAATCACGTTAATCTCTGGGTTATATTTAATTTGCGCGCATTATCGCATATTTCTTCGCGTCTTTATTAATCTCGCGTAAAAATTTGCTAGAAAATCCATGATAAAAAGCGTATAGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATga                                                                                                                                            <  1:114399/251‑1 (MQ=255)                                                                                      aTCACGTTAATCTCTGGGTTATATTTAATTTGCGCGCATTATCGCATATTTCTTCGCGTCTTTATTAATCTCGCGTAAAAATTTGCTAGAAAATCCATGATAAAAAGCGTATAGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGaaa                                                                                                                                       <  1:293654/251‑1 (MQ=255)                                                                                         aCGTTAATCTCTGGGTTATATTTAATTTGCGCGCATTATCGCATATTTCTTCGCGTCTTTATTAATCTCGCGTAAAAATTTGCTAGAAAATCCATGATAAAAAGCGTATAGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATg                                                                                                                                                                                               >  1:45888/1‑192 (MQ=255)                                                                                              aaTCTCTGGGTTATATTTAATTTGCGCGCATTATCGCATATTTCTTCGCGTCTTTATTAATCTCGCGTAAAAATTTGCTAGAAAATCCATGATAAAAAGCGTATAGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTg                                                                                                                               >  1:111217/1‑251 (MQ=255)                                                                                               aTCTCTGGGTTATATTTAATTTGCGCGCATTATCGCATATTTCTTCGCGTCTTTATTAATCTCGCGTAAAAATTTGCTAGAAAATCCATGATAAAAAGCGTATAGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTg                                                                                                                               >  1:857960/1‑250 (MQ=255)                                                                                                tctcTGGGTTATATTTAATTTGCGCGCATTATCGCATATTTCTTCGCGTCTTTATTAATCTCGCGTAAAAATTTGCTAGAAAATCCATGATAAAAAGCGTATAGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAatat                                                                                                                                                                                                                                  <  1:562159/150‑1 (MQ=255)                                                                                                tctcTGGGTTATATTTAATTTGCGCGCATTATCGCATATTTCTTCGCGTCTTTATTAATCTCGCGTAAAAATTTGCTAGAAAATCCATGATAAAAAGCGTATAGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACg                                                                                                                                                                                  <  1:804422/198‑1 (MQ=255)                                                                                                tctcTGGGTTATATTTAATTTGCGCGCATTATCGCATATTTCTTCGCGTCTTTATTAATCTCGCGTAAAAATTTGCTAGAAAATCCATGATAAAAAGCGTATAGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCa                                                                                                                             >  1:488963/1‑251 (MQ=255)                                                                                                 ctctGGGTTATATTTAATTTGCGCGCATTATCGCATATTTCTTCGCGTCTTTATTAATCTCGCGTAAAAATTTGCTAGAAAATCCATGATAAAAAGCGTATAGTGACACAGTTACCACGTCACTACAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGa                                                                                                                                                                                 >  1:467228/1‑198 (MQ=255)                                                                                                   ctGGGTTATATTTAATTTGCGCGCATTATCGCATATTTCTTCGCGTCTTTATTAATCTCGCGTGAAAATTTGCTAGAAAATCCATGATAAAAAGCGTATAGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGa                                                                                                                          >  1:359395/1‑251 (MQ=255)                                                                                                     gggTTATATTTAATTTGCGCGCATTATCGCATATTTCTTCGCGTCTTTATTAATCTCGCGTAAAAATTTGCTAGAAAATCCATGATAAAAAGCGTATAGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATGTCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATga                                                                                                                                            >  1:66823/1‑231 (MQ=255)                                                                                                       gTTATATTTAATTTGCGCGCATTATCGCATATTTCTTCGCGTCTTTATTAATCTCGCGTAAAAATTTGCTAGAAAATCCATGATAAAAAGCGTATAGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGa                                                                                                                       >  1:877763/1‑250 (MQ=255)                                                                                                           tatTTAATTTGCGCGCATTATCGCATATTTCTTCGCGTCTTTATTAATCTCGCGTAAAAATTTGCTAGAAAATCCATGATAAAAAGCGTATAGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGcc                                                                                                                                                                         >  1:621304/1‑196 (MQ=255)                                                                                                           tatTTAATTTGCGCGCATTATCGCATATTTCTTCGCGTCTTTATTAATCTCGCGTAAAAATTTGCTAGAAAATCCATGATAAAAAGCGTATAGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATga                                                                                                                                            >  1:11543/1‑225 (MQ=255)                                                                                                                            ttATCGCATATTTCTTCGCGTCTTTATTAATCTCGCGTAAAAATTTGCTAGAAAATCCATGATAAAAAGCGTATAGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCgg                                                                                                  >  1:42623/1‑250 (MQ=255)                                                                                                                                 gCATATTTCTTCGCGTCTTTATTAATCTCGCGTAAAAATTTGCTAGAAAATCCATGATAAAAAGCGTATAGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGCCCGTGAAGGc                                                                                                                                                                <  1:810676/183‑1 (MQ=255)                                                                                                                                          ttcGCGTCTTTATTAATCTCGCGTAAAAATTTGCTAGAAAATCCATGATAAAAAGCGTATAGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTAccc                                                                                   <  1:813188/251‑1 (MQ=255)                                                                                                                                                tCTTTATTAATCTCGCGTAAAAATTTGCTAGAAAATCCATGATAAAAAGCGTATAGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATggg                                                                                                                                                                                             <  1:267855/139‑1 (MQ=255)                                                                                                                                                 cTTTATTAATCTCGCGTAAAAATTTGCTAGAAAATCCATGATAAAAAGCGTATAGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCaaaaa                                                                            >  1:458340/1‑251 (MQ=255)                                                                                                                                                   ttattaATCTCGCGTAAAAATTTGCTAGAAAATCCATGATAAAAAGCGTATAGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCaaaacat                                                                          >  1:629926/1‑248 (MQ=255)                                                                                                                                                    tattaATCTCGCGTAAAAATTTGCTAGAAAATCCATGATAAAAAGCGTATAGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTAttt                                                                                                                                                                                      >  1:140001/1‑142 (MQ=255)                                                                                                                                                    tattaATCTCGCGTAAAAATTTGCTAGAAAATCCATGATAAAAAGCGTATAGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGCCCGTGAAGGCGAAAACGTTAccc                                                                                                                                                   <  1:113373/177‑1 (MQ=255)                                                                                                                                                                      aTTTGCTAGAAAATCCATGATAAAAAGCGTATAGTTACACAGTAAACACGTCACTAAAACGGTTTGGCAAAAAGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGCCACCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAAcc                                                       <  1:179121/251‑1 (MQ=255)                                                                                                                                                                             aGAAAATCCATGATAAAAAGCGTATAGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAAccc                                                      <  1:330993/245‑1 (MQ=255)                                                                                                                                                                                      aTGATAAAAAGCGTATAGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAAt                                       >  1:830994/1‑251 (MQ=255)                                                                                                                                                                                                     taGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAg                        >  1:332347/1‑251 (MQ=255)                                                                                                                                                                                                     taGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAg                        >  1:681067/1‑251 (MQ=255)                                                                                                                                                                                                     taGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAg                        >  1:233095/1‑251 (MQ=255)                                                                                                                                                                                                       gTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGa                       >  1:227376/1‑250 (MQ=255)                                                                                                                                                                                                       gTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGa                       >  1:193176/1‑250 (MQ=255)                                                                                                                                                                                                        tGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTGCCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGAcc                     <  1:855565/251‑1 (MQ=255)                                                                                                                                                                                                        tGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGAcc                     <  1:220933/251‑1 (MQ=255)                                                                                                                                                                                                        tGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGAcc                     <  1:163273/251‑1 (MQ=255)                                                                                                                                                                                                        tGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGAc                      <  1:571886/250‑1 (MQ=255)                                                                                                                                                                                                             caGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTgg                                    <  1:660669/231‑1 (MQ=255)                                                                                                                                                                                                             caGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGAcc                     >  1:829976/1‑246 (MQ=255)                                                                                                                                                                                                             caGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGAcc                     >  1:433588/1‑246 (MQ=255)                                                                                                                                                                                                               gTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTgaag                                                                     <  1:832328/196‑1 (MQ=255)                                                                                                                                                                                                               gTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCAt              >  1:209162/1‑251 (MQ=255)                                                                                                                                                                                                                        tCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAgg                                                                                                                           >  1:540269/1‑133 (MQ=255)                                                                                                                                                                                                                           cTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAAc                                                                                                         <  1:184943/148‑1 (MQ=255)                                                                                                                                                                                                                           cTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGAcc                     <  1:85007/232‑1 (MQ=255)                                                                                                                                                                                                                           cTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCagaag  >  1:742443/1‑251 (MQ=255)                                                                                                                                                                                                                           cTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCagaag  <  1:38410/251‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               CTGAGGTTGTGAGGGAATTTCAGTCACGGCTAATTCCGGACGTAACGCCTGCCAAGGCAATGTGTTATTAGATAAAGAAGATAAAATCACGTTAATCTCTGGGTTATATTTAATTTGCGCGCATTATCGCATATTTCTTCGCGTCTTTATTAATCTCGCGTAAAAATTTGCTAGAAAATCCATGATAAAAAGCGTATAGTGACACAGTTACCACGTCACTAAAACGGTTTAGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTTAACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCAGAAG  >  1/1395939‑1396407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑