Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,393,407	G→A	intergenic (‑20/+19898)	unknown ← / ← unknown	tRNA‑Val‑GAC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,393,407	0	G	A	100.0%	164.9 / NA	59	intergenic (‑20/+19898)	unknown/unknown	tRNA‑Val‑GAC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/25);  total (34/25)

ATCAAAAACAGTAGAAAATATTAATTTTCCATTGTGATTTCCAACCTGAAAAAATAAAGACGATAAAAAGTTAAGAAAGGCATTTTCGGCTGATTTTAGCAAAAAACTGGTGGCGTTGCGACGGATTTGGGGAAATAAACAATGGTGCGACCGGCTGGACTCGAACCAGTGACCCCCACCATGTCAAGGTGGTGCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGGTAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAACACCGAAAAAACAAACCGCACTTTCTTTCAGAAAAAAGTACGGTGAAAAATTTCAGTGTTTTTTAAGACTGCGAATTAGAAACTTGAGGTATCTTGAAACAAGCCGACTTTCAAGTCCGTTGCGGTGTAAATAACACGACCGTCCACTTCCA  >  1/1393169‑1393647                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                 atCAAAAACAGTAGAAAATATTAATTTTCCATTGTGATTTCCAACCTGAAAAAATAAAGACGATAAAAAGTTAAGAAAGGCATTTTCGGCTGATTTTAGCAAAAAACTGGTGGCGTTGCGACGGATTTGGGGAAATAAACAATGGTGCGACCGGCTGGACTCGAACCAGTGACCCCCACCATGTCAAGGTGGTGCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCtttt                                                                                                                                                                                                                                      >  1:835584/1‑251 (MQ=255)         cAGTAGAAAATATTAATTTTCCATTGTGATTTCCAACCTGAAAAAATAAAGACGATAAAAAGTTAAGAAAGGCATTTTCGGCTGATTTTAGCAAAAAACTGGTGGCGTTGCGACGGATTTGGGGAAATAAACAATGGTGCGACCGGCTGGACTCGAACCAGTGACCCCCACCATGTCAAGGCGGTGCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTaa                                                                                                                                                                                                                              >  1:385717/1‑251 (MQ=255)         cAGTAGAAAATATTAATTTTCCATTGTGATTTCCAACCTGAAAAAATAAAGACGATAAAAAGTTAAGAAAGGCATTTTCGGCCGATTTTAGCAAAAAACTGGTGGCGTTGCGACGGATTTGGGGAAATAAACAATGGTGCGACCGGCTGGACTCGAACCAGTGACCCCCACCATGTCAAGGTGGTGCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTaa                                                                                                                                                                                                                              >  1:386734/1‑251 (MQ=255)           gTAGAAAATATTAATTTTCCATTGTGATTTCCAACCTGAAAAAATAAAGACGATAAAAAGTTAAGAAAGGCATTTTCGGCTGATTTTAGCAAAAAACTGGTGGCGTTGCGACGGATTTGGGGAAATAAACAATGGTGCGACCGGCTGGACTCGAACCAGTGACCCCCACCATGTCAAGGTGGTGCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTaaaa                                                                                                                                                                                                                            >  1:151341/1‑251 (MQ=255)                   tatTAATTTTCCATTGTGATTTCCAACCTGAAAAAATAAAGACGATAAAAAGTTAAGAAAGGCATTTTCGGCTGATTTTAGCAAAAAACTGGTGGCGTTGCGACGGATTTGGGGAAATAAACAATGGTGCGACCGGCTGGACTCGAACCAGTGACCCCCACCATGTCAAGGTGGTGCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTACCTTTTCGGGCTAAAACAAGtttt                                                                                                                                                                                                                    >  1:398269/1‑251 (MQ=255)                        aTTTTCCATTGTGATTTCCAACCTGAAAAAATAAAGACGATAAAAAGTTAAGAAAGGCATTTTCGGCTGATTTTAGCAAAAAACTGGTGGCGTTGCGACGGATTTGGGGAAATAAACAATGGTGCGACCGGCTGGACTCGAACCAGTGACCCCCACCATGTCAAGGTGGTGCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTAtt                                                                                                                                                                                                               <  1:697343/251‑1 (MQ=255)                             ccATTGTGATTTCCAACCTGAAAAAATAAAGACGATAAAAAGTTAAGAAAGGCATTTTCGGCTGATTTTAGCAAAAAACTGGTGGCGTTGCGACGGATTTGGGGAAATAAACAATGGTGCGACCGGCTGGACTCGAACCAGTGACCCCCACCATGTCAAGGTGGTGCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGAtagc                                                                                                                                                                                                                                              >  1:260915/1‑215 (MQ=255)                                                   aaaaTAAAGACGATAAAAAGTTAAGAAAGGCATTTTCGGCTGATTTTAGCAAAAAACTGGTGGCGTTGCGACGGATTTGGGGAAATAAACAATGGTGCGACCGGCTGGACTCGAACCAGTGACCCCCACCATGTCAAGGTGGTGCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAAt                                                                                                                                                                                    <  1:457409/251‑1 (MQ=255)                                                      aTAAAGACGATAAAAAGTTAAGAAAGGCATTTTCGGCTGATTTTAGCAAAAAACTGGTGGCGTTGCGACGGATTTGGGGAAATAAACAATGGTGCGACCGGCTGGACTCGAACCAGTGACCCCCACCATGTCAAGGTGGTGCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTaa                                                                                                                                                                                 >  1:35579/1‑251 (MQ=255)                                                        aaaGACGATAAAAAGTTAAGAAAGGCATTTTCGGCTGATTTTAGCAAAAAACTGGTGGCGTTGCGACGGATTTGGGGAAATAAACAATGGTGCGACCGGCTGGACTCGAACCAGTGACCCCCACCATGTCAAGGTGGTGCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAAcc                                                                                                                                                                               <  1:470893/251‑1 (MQ=255)                                                        aaaGACGATAAAAAGTTAAGAAAGGCATTTTCGGCTGATTTTAGCAAAAAACTGGTGGCGTTGCGACGGATTTGGGGAAATAAACAATGGTGCGACCGGCTGGACTCGAACCAGTGACCCCCACCATGTCAAGGTGGTGCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAAcc                                                                                                                                                                               <  1:759771/251‑1 (MQ=255)                                                                  aaaaGTTAAGAAAGGCATTTTCGGCTGATTTTAGCAAAAAACTGGTGGCGTTGCGACGGATTTGGGGAAATAAACAATGGTGCGACCGGCTGGACTCGAACCAGTGACCCCCACCATGTCAAGGTGGTGCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTAt                                                                                                                                                                     <  1:499077/251‑1 (MQ=255)                                                                  aaaaGTTAAGAAAGGCATTTTCGGCTGATTTTAGCAAAAAACTGGTGGCGTTGCAACGGATTTGGGGAAATAAACAATGGTGCGACCGGCTGGACTCGAACCAGTGACCCCCACCATGTCAAGGTGGTGCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTAt                                                                                                                                                                     <  1:57773/251‑1 (MQ=255)                                                                    aaGTTAAGAAAGGCATTTTCGGCTGATTTTAGCAAAAAACTGGTGGCGTTGCGACGGATTTGGGGAAATAAACAATGGTGCGACCGGCTGGACTCGAACCAGTGACCCCCACCATGTCAAGGTGGTGCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGa                                                                                                                                                                   >  1:61179/1‑251 (MQ=255)                                                                      gTTAAGAAAGGCATTTTCGGCTGATTTTAGCAAAAAACTGGTGGCGTTGCGACGGATTTGGGGAAATAAACAATGGTGCGACCGGCTGGACTCGAACCAGTGACCCCCACCATGTCAAGGTGGTGCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTAt                                                                                                                                                                     <  1:303436/247‑1 (MQ=255)                                                                      gTTAAGAAAGGCATTTTCGGCTGATTTTAGCAAAAAACTGGTGGCGTTGCGACGGATTTGGGGAAATAAACAATGGTGCGACCGGCTGGACTCGAACCAGTGACCCCCACCATGTCAAGGTGGTGCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAg                                                                                                                                                                 >  1:325354/1‑251 (MQ=255)                                                                                            gATTTTAGCAAAAAACTGGTGGCGTTGCGACGGATTTGGGGAAATAAACAATGGTGCGACCGGCTGGACTCGAACCAGTGACCCCCACCATGTCAAGGCGGTGCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAac                                                                                                                                                         >  1:36951/1‑237 (MQ=255)                                                                                                           cTGGTGGCGTTGCGACGGATTTGGGGAAATAAACAATGGTGCGACCGGCTGGACTCGAACCAGTGACCCCCACCATGTCAAGGTGGTGCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATg                                                                                                                                                                    <  1:710865/211‑1 (MQ=255)                                                                                                               tggCGTTGCGACGGATTTGGGGAAATAAACAATGGTGCGACCGGCTGGACTCGAACCAGTGACCCCCACCATGTCAAGGTGGTGCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAACACCGAAAAAACAAACCGCACTTTCTTTCAGaa                                                                                                                         <  1:676228/250‑1 (MQ=255)                                                                                                                       cGACGGATTTGGGGAAATAAACAATGGTGCGACCGGCTGGACTCGAACCAGTGACCCCCACCATGTCAAGGTGGTGCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAACACCGAAAAAACAAACCGCACTTTCTTTCAGAAAAAAGTAc                                                                                                                 <  1:427639/250‑1 (MQ=255)                                                                                                                            gATTTGGGGAAATAAACAATGGTGCGACCGGCTGGACTCGAACCAGTGACCCCCACCATGTCAAGGTGGTGCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAACACCGAAAAAACAAACCGCACTTTCTTTCAGAAAAAAGTACGGTGaa                                                                                                           >  1:224655/1‑251 (MQ=255)                                                                                                                                       aTAAACAATGGTGCGACCGGCTGGACTCGAACCAGTGACCCCCACCATGTCAAGGTGGTGCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAACACCGAAAAAACAAACCGCACTTTCTTTCAGAAAAAAGTACGGTGAAAAATTTCAgtg                                                                                                >  1:509478/1‑251 (MQ=255)                                                                                                                                       aTAAACAATGGTGCGACCGGCTGGACTCGAACCAGTGACCCCCACCATGTCAAGGTGGTGCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAACACCGAAAAAACAAACCGCACTTTCTTTCAGAAAAAAGTACGGTGAAAAATTTCAgtg                                                                                                >  1:760755/1‑251 (MQ=255)                                                                                                                                               tGGTGCGACCGGCTGGACTCGAACCAGTGACCCCCACCATGTCAAGGTGGTGCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAACACCGAAAAAACAAACCGCACTTTCTTTCAGAAAAAAGTACGGTGAAAAATTTCAGTGTTTTTTaa                                                                                        >  1:805008/1‑251 (MQ=255)                                                                                                                                                 gTGCGACCGGCTGGACTCGAACCAGTGACCCCCACCATGTCAAGGTGGTGCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAACACCGAAAAAACAAACCGCACTTTCTTTCAGAAAAAAGTACGGTGAAAAATTTCAGTGTTTTTTAAg                                                                                       <  1:670577/250‑1 (MQ=255)                                                                                                                                                      aCCGGCTGGACTCGAACCAGTGACCCCCACCATGTCAAGGTGGTGCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAACACCGAAAAAACAAACCGCACTTTCTTTCAGAAAAAAGTACGGTGAAAAATTTCAGTGTTTTTTAAGACTGCg                                                                                 >  1:124536/1‑251 (MQ=255)                                                                                                                                                         ggCTGGACTCGAACCAGTGACCCCCACCATGTCAAGGTGGTGCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAacac                                                                                                                                                       >  1:435398/1‑178 (MQ=255)                                                                                                                                                          gCTGGACTCGAACCAGTGACCCCCACCATGTCAAGGTGGTGCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAACACCGAAAAAACAAACCGCACTTTCTTTCAGAAAAAAGTACGGTGAAAAATTTCAGTGTTTTTTAAGACTGCGAAt                                                                              <  1:635428/250‑1 (MQ=255)                                                                                                                                                                    aaCCAGTGACCCCCACCATGTCAAGGTGGTGCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAACACCGAAAAAACAAACCGCACTTTCTTTCAGAAAAAAGTACGGTGAAAAATTTCAGTGTTTTTTAAGACTGCGa                                                                                <  1:878547/238‑1 (MQ=255)                                                                                                                                                                      ccAGTGACCCCCACCATGTCAAGGTGGTGCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAACACCGAAAAAACAAACCGCACTTTCTTTCAGAAAAAAGTACGGTGAAAAATTTCAGTGTTTTTTAAGACTGCGAATTAGAAACTTGAgg                                                                 >  1:400407/1‑251 (MQ=255)                                                                                                                                                                       cAGTGACCCCCACCATGTCAAGGTGGTGCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAACACCGAAAAAACAAACCGCACTTTCTTTCAGAAAAAAGt                                                                                                                   >  1:621403/1‑200 (MQ=255)                                                                                                                                                                       cAGTGACCCCCACCATGTCAAGGTGGTGCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAACACCGAAAAAACAAACCGCACTTTCTTTCAGAAAAAAGTACGGTGAAAAATTTCAGTGTTTTTTAAGACTGCGAATTAGAAACTTGAGGt                                                                >  1:744980/1‑251 (MQ=255)                                                                                                                                                                         gTGACCCCCACCATGTCAAGGTGGTGCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAACACCGAAAAAACAAACCGCACTTTCTTTCAGAAAAAAGTACGGTGAAAAATTTCAGTGTTTTTTAAg                                                                                       >  1:363351/1‑226 (MQ=255)                                                                                                                                                                             cccccACCATGTCAAGGTGGTGCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTAt                                                                                                                                                                     <  1:409527/144‑1 (MQ=255)                                                                                                                                                                                   ccaTGTCAAGGTGGTGCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAACACCGAAAAAACAAACCGCACTTTCTTTCAGAAAAAAGTACGGTGAAAAATTTCAGTGTTTTTTAAGACTGCGAATTAGaa                                                                         <  1:233527/230‑1 (MQ=255)                                                                                                                                                                                     aTGTCAAGGTGGTGCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAACACCGAAAAAACAAACCGCACTTTCTTTCAGAAAAAAGTACGGTGAAAAATTTCAGTGTTTTTTAAGACTGCGAATTAGAAACTTGAGGTATCTTGAAACAAg                                                   >  1:634431/1‑250 (MQ=255)                                                                                                                                                                                     aTGTCAAGGTGGTGCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAACACCGAAAAAACAAACCGCACTTTCTTTCAGAAAAAAGTACGGTGAAAAATTTCAGTGTTTTTTAAGACTGCGAATTAGAAACTTGAGGTATCTTGAAACAAg                                                   >  1:300148/1‑250 (MQ=255)                                                                                                                                                                                      tGTCAAGGTGGTGCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAACACCGAAAAAACAAACCGCACtttctttc                                                                                                                             <  1:75862/175‑1 (MQ=255)                                                                                                                                                                                      tGTCAAGGTGGTGCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAACACCGAAAAAACAAACCGCACTTTCTTTCAGAAAAAAGTACGGTGAAAAATTTCAGTGTTTTTTAAg                                                                                       >  1:499651/1‑213 (MQ=255)                                                                                                                                                                                      tGTCAAGGTGGTGCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAACACCGAAAAAACAAACCGCACTTTCTTTCAGAAAAAAGTACGGTGAAAAATTTCAGTGTTTTTTAAGACTGCGAATTAGAAACTTGAGGTATCTTGAAACAAGc                                                  >  1:489401/1‑250 (MQ=255)                                                                                                                                                                                             gtggtgCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAACACCGAAAAAACAAACCGCACTTTCTTTCAGAAAAAAGTACGGTGAAAAATTTCAGTGTTTTTTAAGACTGCGAATTAGAAACTTGAGGTATCTTGAAAc                                                      >  1:133791/1‑239 (MQ=255)                                                                                                                                                                                                  gCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAACACCGAAAAAACAAACCGCACTTTCTTTCAGAAAAAAGTACGGTGAAAAATTTCAg                                                                                                  >  1:184482/1‑190 (MQ=255)                                                                                                                                                                                                  gCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAACACCGAAAAAACAAACCGCACTTTCTTTCAGAAAAAAGTACGGTGAAAAATTTCAGTGTTTTTTAAGAc                                                                                     <  1:550758/203‑1 (MQ=255)                                                                                                                                                                                                  gCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAACACCGAAAAAACAAACCGCACTTTCTTTCAGAAAAAAGTACGGTGAAAAATTTCAGTGTTTTTTAAGACTGCGAATTAGAAACTTGAGGTATCTTGAAACAAGCCGACTTTCAAGt                                      >  1:626454/1‑250 (MQ=255)                                                                                                                                                                                                  gCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAACACCGAAAAAACAAACCGCACTTTCTTTCAGAAAAAAGTACGGTGAAAAATTTCAGTGTTTTTTAAGACTGCGAATTAGAAACTTGAGGTATCTTGAAACAAGCCGACTTTCAAGt                                      >  1:548446/1‑250 (MQ=255)                                                                                                                                                                                                  gCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAACACCGAAAAAACAAACCGCACTTTCTTTCAGAAAAAAGTACGGTGAAAAATTTCAGTGTTTTTTAAGACTGCGAATTAGAAACTTGAGGTATCTTGAAACAAGCCGACTTTCAAGt                                      >  1:348957/1‑250 (MQ=255)                                                                                                                                                                                                     ctAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAACACCGAAAAAACAAACCGCACTTTCTTTCAGAAAAAAGTACGGTGAAAAATTTCAGTGTTTTTTAAGACTGCGAATTAGAAACTTGAGGTATCTTGAAACAAGCCGACTTTCAAGTCCGt                                  >  1:100280/1‑251 (MQ=255)                                                                                                                                                                                                            aCTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAACACCGAAAAAACAAACCGCActttct                                                                                                                                >  1:684534/1‑150 (MQ=255)                                                                                                                                                                                                                aGCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAACACCGAAAAAACAAACCGCACTTTCTTTCAGAAAAAAGTACGGTGAAAAATTTCAGTGTTTTTTAAGAc                                                                                     <  1:133892/189‑1 (MQ=255)                                                                                                                                                                                                                 gCTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAACACCGAAAAAACAAACCGCACTTTCTTTCAGAAAAAAGTACGGTGAAAAATTTCAGTGTTTTTTAAGACTGCGAATTAGAAACTTGAGGTATCTTGAAACAAGCCGACTTTCAAGTCCGTTGCGGTGTAAAt                      >  1:151192/1‑251 (MQ=255)                                                                                                                                                                                                                  cTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAACACCGAAAAAACAAACCGCACTTTCTTTCAGAAAAAAGTACGGTGAAAAATTTCAGTGTTTTTTAAGACTGCGAATTAGAAACTTGAGGTATCTTGAAACAAGCCGACTTTCAAGTCCGTTGCGGTGTAAAt                      <  1:605167/250‑1 (MQ=255)                                                                                                                                                                                                                  cTACGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAACACCGAAAAAACAAACCGCACTTTCTTTCAGAAAAAAGTACGGTGAAAAATTTCAGTGTTTTTTAAGACTGCGAATTAGAAACTTGAGGTATCTTGAAACAAGCCGACTTTCAAGTCCGTTGCGGTGTAAATa                     <  1:140291/251‑1 (MQ=255)                                                                                                                                                                                                                    aCGGTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAACACCGAAAAAACAAACCGCACTTTCTTTCAGAAAAAAGTACGGTGAAAAATTTCAGTGTTTTTTAAGACTGCGAATTAGAAACTTGAGGTATCTTGAAACAAGCCGACTTTCAAGTCCGTTGCGGTGTAAATAac                   <  1:618235/251‑1 (MQ=255)                                                                                                                                                                                                                       gTCGCAGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAACACCGAAAAAACAAACCGCACTTTCTTTCAGAAAAAAGTACGGTGAAAAATTTCAGTGTTTTTTAAGACTGCGAATTAGAAACTTGAGGTATCTTGAAACAAGCCGACTTTCAAGTCCGTTGCGGTGTAAATAacac                 <  1:538435/250‑1 (MQ=255)                                                                                                                                                                                                                            aGTGAAAAGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAACACCGAAAAAACAAACCGCACTTTCTTTCAGAAAAAAGTACGGTGAAAAATTTCAGTGTTTTTTAAGACTGCGAATTAGAAACTTGAGGTATCTTGAAACAAGCCGACTTTCAAGTCCGTTGCGGTGTAAATAac                   <  1:345406/243‑1 (MQ=255)                                                                                                                                                                                                                                aaaaGTGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAACACCGAAAAAACAAACCGCACTTTCTTTCAGAAAAAAGTACGGTGAAAAATTTCAGTGTTTTTTAAGACTGCGAATTAGAAACTTGAGGTATCTTGAAACAAGCCGACTTTCAAGTCCGTTGCGGTGTAAATAACACGACCGtccac       <  1:402927/251‑1 (MQ=255)                                                                                                                                                                                                                                     tGCGCTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAACACCGAAAAAACAAACCGCACTTTCTTTCAGAAAAAAGTACGGTGAAAAATTTCAGTGTTTTTTAAGACTGCGAATTAGAAACTTGAGGTATCTTGAAACAAGCCGACTTTCAAGTCCGTTGCGGTGTAAATAACACGACCGtccacttc    <  1:872234/249‑1 (MQ=255)                                                                                                                                                                                                                                      gcgcTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAACACCGAAAAAACAAACCGCACTTTCTTTCAGAAAAAAGTACGGTGAAAAATTTCAGTGTTTTTTAAGACTGCGAATTAGAAACTTGAGGTATCTTGAAACAAGCCGACTTTCAAGTCCGTTGCGGTGTAAATAACACGACCGtccacttcca  >  1:388768/1‑250 (MQ=255)                                                                                                                                                                                                                                      gcgcTAATGATAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAACACCGAAAAAACAAACCGCACTTTCTTTCAGAAAAAAGTACGGTGAAAAATTTCAGTGTTTTTTAAGACTGCGAATTAGAAACTTGAGGTATCTTGAAACAAGCCGACTTTCAAGTCCGTTGCGGTGTAAATAACACGACCGtccacttcca  >  1:611056/1‑250 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                 ATCAAAAACAGTAGAAAATATTAATTTTCCATTGTGATTTCCAACCTGAAAAAATAAAGACGATAAAAAGTTAAGAAAGGCATTTTCGGCTGATTTTAGCAAAAAACTGGTGGCGTTGCGACGGATTTGGGGAAATAAACAATGGTGCGACCGGCTGGACTCGAACCAGTGACCCCCACCATGTCAAGGTGGTGCTCTAACCAACTGAGCTACGGTCGCAGTGAAAAGTGCGCTAATGGTAGCTAGCTTTTCGGGCTAAAACAAGTTTTTTATTTGATTGGGCTGATGATTGCAAAAAAATTAACCAGTTCATTATGAAGATAAAAACACCGAAAAAACAAACCGCACTTTCTTTCAGAAAAAAGTACGGTGAAAAATTTCAGTGTTTTTTAAGACTGCGAATTAGAAACTTGAGGTATCTTGAAACAAGCCGACTTTCAAGTCCGTTGCGGTGTAAATAACACGACCGTCCACTTCCA  >  1/1393169‑1393647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑