Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,384,636	A→G	intergenic (+245506/+8675)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,384,636	0	A	G	100.0%	124.0 / NA	43	intergenic (+245506/+8675)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (1/42);  total (1/42)

AAATTTTGTTTATACCAAAGCCAAAAGCAGGCTTTAATCTATTCAATCATCAATCCGCCAGAAACAACGGTCCCATTGGCATTTTCGGCAGATTAAAGTTGTCGGGGTAAATGACATTCACCAGATACAACCCTTCCGGTTTCGCGGTGGGCGCGGCGAGGGCGCGGTCTTTTTGCTCCAACAACCATTTCATCCACTCAACGGGTTGATTGCAACAGCCCACTTCAATAAGGCTGCCGACGATATTACGCACCATGTGATGCACAAAAGCGTTAGCTTGAATATCCACGATAATGTACTGCCCCTGACGAGTGACGTTTAAATGATGGACGTTGCGCCATGGGGTGTTGGATTGGCATTGAGCGGCGCGGAAGGAGCTGAAATCATTTTCGCCGAGCAGCGCCTGTCCCGCGTCGTGCATTAATTGATGATCGAGTTCTAAATGGCAATGGGTTACGCCTTGCGGCAAAATCGCCG  >  1/1384392‑1384868                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                         aaaTTTTGTTTATACCAAAGCCAAAAGCAGGCTTTAATCTATTCAATCATCAATCCGCCAGAAACAACGGTCCCATTGGCAGTTTCGGCAGATTAAAGTTGTCGGGGTAAATGACATTCACCAGATACAACCCTTCCGGTTTCGCGGTGGGCGCGGCGAGGGCGCGGTCTTTTTGCTCCAACAACCATTTCATCCACTTAACGGGTTGATTGCAACAGCCCACTTCAATAAGGCTGCCGACGATGTTACGc                                                                                                                                                                                                                                    <  1:428207/251‑1 (MQ=255)   aTTTTGTTTATACCAAAGCCAAAAGCAGGCTTTAATCTATTCAATCATCAATCCGCCAGAAACAACGGTCCCATTGGCATTTTCGGCAGATTAAAGTTGTCGGGGTAAATGACATTCACCAGATACAACCCTTCCGGTTTCGCGGTGGGCGCGGCGAGGGCGCGGTCTTTTTGCTCCAACAACCATTTCATCCACTTAACGGGTTGATTGCAACAGCCCACTTCAATAAGGCTGCCGACGATGTTACGCAc                                                                                                                                                                                                                                  <  1:163733/251‑1 (MQ=255)   aTTTTGTTTATACCAAAGCCAAAAGCAGGCTTTAATCTATTCAATCATCAATCCGCCAGAAACAACGGTCCCATTGGCATTTTCGGCAGATTAAAGTTGTCGGGGTAAATGACATTCACCAGATACAACCCTTCCGGTTTCGCGGTGGGCGCGGCGAGGGCGCGGTCTTTTTGCTCCAACAACCATTTCATCCACTTAACGGGTTGATTGCAACAGCCCACTTCAATAAGGCTGCCGACGATGTTACGCAc                                                                                                                                                                                                                                  <  1:468872/251‑1 (MQ=255)   aTTTTGTTTATACCAAAGCCAAAAGCAGGCTTTAATCTATTCAATCATCAATCCGCCAGAAACAACGGTCCCATTGGCATTTTCGGCAGATTAAAGTTGTCGGGGTAAATGACATTCACCAGATACAACCCTTCCGGTTTCGCGGTGGGCGCGGCGAGGGCGCGGTCTTTTTGCTCCAACAACCATTTCATCCACTTAACGGGTTGATTGCAACAGCCCACTTCAATAAGGCTGCCGACGATGTTACGCAc                                                                                                                                                                                                                                  <  1:266253/251‑1 (MQ=255)        gTTTATACCAAAGCCAAAAGCAGGCTTTAATCTATTCAATCATCAATCCGCCAGAAACAACGGTCCCATTGGCATTTTCGGCAGATTAAAGTTGTCGGGGTAAATGACATTCACCAGATACAACCCTTCCGGTTTCGCGGTGGGCGCGGCGAGGGCGCGGTCTTTTTGCTCCAACAACCATTTCATCCACTTAACGGGTTGATTGCAACAGCCCACTTCAATAAGGCTGCCGACGATGTTAc                                                                                                                                                                                                                                      <  1:233214/242‑1 (MQ=255)         tttATACCAAAGCCAAAAGCAGGCTTTAATCTATTCAATCATCAATCCGCCAGAAACAACGGTCCCATTGGCATTTTCGGCAGATTAAAGTTGTCGGGGTAAATGACATTCACCAGATACAACCCTTCCGGTTTCGCGGTGGGCGCGGCGAGGGCGCGGTCTTTTTGCTCCAACAACCATTTCATCCACTTAACGGGTTGATTGCAACAGCCCACTTCAATAAGGCTGCCGACGATGTTACGCACCAtgt                                                                                                                                                                                                                             <  1:811933/250‑1 (MQ=255)                                 tttAATCTATTCAATCATCAATCCGCCAGAAACAACGGTCCCATTGGCATTTTCGGCAGATTAAAGTTGTCGGGGTAAATGACATTCACCAGATACAACCCTTCCGGTTTCGCGGTGGGCGCGGCGAGGGCGCGGTCTTTTTGCTCCAACAACCATTTCATCCACTTAACGGGTTGATTGCAACAGCCCACTTCAATAAGGCTGCCGACGATGTTACGCACCATGTGATGCACAAAAGCGTTAGCTTGAat                                                                                                                                                                                                    <  1:784667/251‑1 (MQ=255)                                         aTTCAATCATCAATCCGCCAGAAACAACGGTCCCATTGGCATTTTCGGCAGATTAAAGTTGTCGGGGTAAATGACATTCACCAGATACAACCCTTCCGGTTTCGCGGTGGGCGCGGCGAGGGCGCGGTCTTTTTGCTCCAACAACCATTTCATCCACTTAACGGGTTGATTGCAACAGCCCACTTCAATAAGGCTGCCGACGATGTTACGCACCATGTGATGc                                                                                                                                                                                                                        >  1:70926/1‑223 (MQ=255)                                          ttCAATCATCAATCCGCCAGAAACAACGGTCCCATTGGCATTTTCGGCAGATTAAAGTTGTCGGGGTAAATGACATTCACCAGATACAACCCTTCCGGTTTCGCGGTGGGCGCGGCGAGGGCGCGGTCTTTTTGCTCCAACAACCATTTCATCCACTTAACGGGTTGATTGCAACAGCCCACTTCAATAAGGCTGCCGACGATGTTACGCAc                                                                                                                                                                                                                                  <  1:565112/212‑1 (MQ=255)                                               tcatcaATCCGCCAGAAACAACGGTCCCATTGGCATTTTCGGCAGATTAAAGTTGTCGGGGTAAATGACATTCACCAGATACAACCCTTCCGGTTTCGCGGTGGGCGCGGCGAGGGCGCGGTCTTTTTGCTCCAACAACCATTTCATCCACTTAACGGGTTGATTGCAACAGCCCACTTCAATAAGGCTGCCGACGATGTTACGCACCATGTGATGCACAAAAGCGTTAGCTTGAATATCCACGATAATGt                                                                                                                                                                                      <  1:367735/251‑1 (MQ=255)                                               tcatcaATCCGCCAGAAACAACGGTCCCATTGGCATTTTCGGCAGATTAAAGTTGTCGGGGTAAATGACATTCACCAGATACAACCCTTCCGGTTTCGCGGTGGGCGCGGCGAGGGCGCGGTCTTTTTGCTCCAACAACCATTTCATCCACTTAACGGGTTGATTGCAACAGCCCACTTCAATAAGGCTGCCGACGATGTTACGCACCATGTGATGCACAAAAGCGTTAGCTTGAATATCCACGATAATGt                                                                                                                                                                                      <  1:534665/251‑1 (MQ=255)                                               tcatcaATCCGCCAGAAACAACGGTCCCATTGGCATTTTCGGCAGATTAAAGTTGTCGGGGTAAATGACATTCACCAGATACAACCCTTCCGGTTTCGCGGTGGGCGCGGCGAGGGCGCGGTCTTTTTGCTCCAACAACCATTTCATCCACTTAACGGGTTGATTGCAACAGCCCACTTCAATAAGGCTGCCGACGATGTTACGCACCATGTGATGCACAAAAGCGTTAGCTTGAATATCCACGATAATGt                                                                                                                                                                                      <  1:509207/251‑1 (MQ=255)                                               tcatcaATCCGCCAGAAACAACGGTCCCATTGGCATTTTCGGCAGATTAAAGTTGTCGGGGTAAATGACATTCACCAGATACAACCCTTCCGGTTTCGCGGTGGGCGCGGCGAGGGCGCGGTCTTTTTGCTCCAACAACCATTTCATCCACTTAACGGGTTGATTGCAACAGCCCACTTCAATAAGGCTGCCGACGATGTTACGCACCATGTGATGCACAAAAGCGTTAGCTTGAATATCCACGATAATGt                                                                                                                                                                                      <  1:871869/251‑1 (MQ=255)                                                 atcaATCCGCCAGAAACAACGGTCCCATTGGCATTTTCGGCAGATTAAAGTTGTCGGGGTAAATGACATTCACCAGATACAACCCTTCCGGTTTCGCGGTGGGCGCGGCGAGGGCGCGGTCTTTTTGCTCCAACAACCATTTCATCCACTTAACGGGTTGATTGCAACAGCCCACTTCAATAAGGCTGCCGACGATGTTACGCACCATGTGATGCACAAAAGCGTTAGCTTGAATATCCACGATAATGTAc                                                                                                                                                                                    <  1:273603/251‑1 (MQ=255)                                                       ccGCCAGAAACAACGGTCCCATTGGCATTTTCGGCAGATTAAAGTTGTCGGGGTAAATGACATTCACCAGATACAACCCTTCCGGTTTCGCGGTGGGCGCGGCGAGGGCGCGGTCTTTTTGCTCCAACAACCATTTCATCCACTTAACGGGTTGATTGCAACAGCCCACTTCAATAAGGCTGCCGACGATGTTACGCACCATGTGATGCACAAAAGCGTTAGCTTGAATATCCACGATAATGTACTGcccc                                                                                                                                                                              <  1:556808/251‑1 (MQ=255)                                                             gAAACAACGGTCCCATTGGCATTTTCGGCAGATTAAAGTTGTCGGGGTAAATGACATTCACCAGATACAACCCTTCCGGTTTCGCGGTGGGCGCGGCGAGGGCGCGGTCTTTTTGCTCCAACAACCATTTCATCCACTTAACGGGTTGATTGCAACAGCCCACTTCAATAAGGCTGCCGACGATGTTACGCACCATGTGATGCACAAAAGCGTTAGCTTGAATATCCACGATAATGTACTGCCCCTGACg                                                                                                                                                                         <  1:847183/250‑1 (MQ=255)                                                              aaaCAACGGTCCCATTGGCATTTTCGGCAGATTAAAGTTGTCGGGGTAAATGACATTCACCAGATACAACCCTTCCGGTTTCGCGGTGGGCGCGGCGAGGGCGCGGTCTTTTTGCTCCAACAACCATTTCATCCACTTAACGGGTTGATTGCAACAGCCCACTTCAATAAGGCTGCCGACGATGTTACGCACCATGTGATGCACAAAAGCGTTAGCTTGAATATCCACGATAATGTACTGCCCCTGACg                                                                                                                                                                         <  1:754283/249‑1 (MQ=255)                                                                 caacGGTCCCATTGGCATTTTCGGCAGATTAAAGTTGTCGGGGTAAGTGACATTCACCAGATACAACCCTTCCGGTTTCGCGGTGGGCGCGGCGAGGGCGCGGTCTTTTTGCTCCAACAACCATTTCATCCACTTAACGGGTTGATTGCAACAGCCCGCTTCAATAAGGCTGCCGACGATGTTACGCACCATGTGATGCACAAAAGCGTTAGCTTGAATATCCACGATAATGTACTGCCCCTGACGAGTGa                                                                                                                                                                    <  1:200696/251‑1 (MQ=255)                                                                         ccATTGGCATTTTCGGCAGATTAAAGTTGTCGGGGTAAATGACATTCACCAGATACAACCCTTCCGGTTTCGCGGTGGGCGCGGCGAGGGCGCGGTCTTTTTGCTCCAACAACCATTTCATCCACTTAACGGGTTGATTGCAACAGCCCACTTCAATAAGGCTGCCGACGATGTTACGCAc                                                                                                                                                                                                                                  <  1:38724/181‑1 (MQ=255)                                                                                  ttttCGGCAGATTAAAGTTGTCGGGGTAAATGACATTCACCAGATACAACCCTTCCGGTTTCGCGGTGGGCGCGGCGAGGGCGCGGTCTTTTTGCTCCAACAACCATTTCATCCACTTAACGGGTTGATTGCAACAGCCCACTTCAATAAGGCTGCCGACGATGTTACGCACCATGTGATGCACAAAAGCGTTAGCTTGAATATCCACGATAATGTACTGCCCCTGACGAGTGACGTTTAAATGATGGACg                                                                                                                                                   <  1:883034/251‑1 (MQ=255)                                                                                                       cGGGGTAAATGACATTCACCAGATACAACCCTTCCGGTTTCGCGGTGGGCGCGGCGAGGGCGCGGTCTTTTTGCTCCAACAACCATTTCATCCACTTAACGGGTTGATTGCAACAGCCCACTTCAATAAGGCTGCCGACGATGTTACGCACCATGTGATGCACAAAAGCGTTAGCTTGAATATCCACGATAATGTACTGCCCCTGACGAGTGACGTTTAAATGATGGACGTTGCGCCATGGGGTGTTGGAt                                                                                                                              <  1:250161/251‑1 (MQ=255)                                                                                                       cGGGGTAAATGACATTCACCAGATACAACCCTTCCGGTTTCGCGGTGGGCGCGGCGAGGGCGCGGTCTTTTTGCTCCAACAACCATTTCATCCACTTAACGGGTTGATTGCAACAGCCCACTTCAATAAGGCTGCCGACGATGTTACGCACCATGTGATGCACAAAAGCGTTAGCTTGAATATCCACGATAATGTACTGCCCCTGACGAGTGACGTTTAAATGATGGACGTTGCGCCATGGGGTGTTGGAt                                                                                                                              <  1:85365/251‑1 (MQ=255)                                                                                                         gggTAAATGACATTCACCAGATACAACCCTTCCGGTTTCGCGGTGGGCGCGGCGAGGGCGCGGTCTTTTTGCTCCAACAACCATTTCATCCACTTAACGGGTTGATTGCAACAGCCCACTTCAATAAGGCTGCCGACGATGTTACGCACCATGTGATGCACAAAAGCGTTAGCTTGAATATCCACGATAATGTAc                                                                                                                                                                                    <  1:264537/195‑1 (MQ=255)                                                                                                             aaaTGACATTCACCAGATACAACCCTTCCGGTTTCGCGGTGGGCGCGGCGAGGGCGCGGTCTTTTTGCTCCAACAACCATTTCATCCACTTAACGGGTTGATTGCAACAGCCCACTTCAATAAGGCTGCCGACGATGTTACGCACCATGTGATGCACAAAAGCGTTAGCTTGAATATCCACGATAATGTACTGCCCCTGACGAGTGACGTTTAAATGATGGACGTTGCGCCATGGGGTGTTGGATTGGCAt                                                                                                                        <  1:773272/251‑1 (MQ=255)                                                                                                                  aCATTCACCAGATACAACCCTTCCGGTTTCGCGGTGGGCGCGGCGAGGGCGCGGTCTTTTTGCTCCAACAACCATTTCATCCACTTAACGGGTTGATTGCAACAGCCCACTTCAATAAGGCTGCCGACGATGTTACGCACCATGTGATGCACAAAAGCGTTAGCTTGAATATCCACGATAATGTACTGCCCCTGACGAGTGACGTTTAAATGATGGACGTTGCGCCATGGGGTGTTGGATTGGCATTGAgc                                                                                                                   <  1:468437/251‑1 (MQ=255)                                                                                                                           aGATACAACCCTTCCGGTTTCGCGGTGGGCGCGGCGAGGGCGCGGTCTTTTTGCTCCAACAACCATTTCATCCACTTAACGGGTTGATTGCAACAGCCCACTTCAATAAGGCTGCCGACGATGTTACGCACCATGTGATGCACAAAAGCGTTAGCTTGAATATCCACGATAATGTACTGCCCCTGACGAGTGACGTTTAAat                                                                                                                                                           <  1:295206/202‑1 (MQ=255)                                                                                                                            gATACAACCCTTCCGGTTTCGCGGTGGGCGCGGCGAGGGCGCGGTCTTTTTGCTCCAACAACCATTTCATCCACTTAACGGGTTGATTGCAACAGCCCACTTCAATAAGGCTGCCGACGATGTTACGCACCATGTGATGc                                                                                                                                                                                                                        <  1:494527/140‑1 (MQ=255)                                                                                                                             aTACAACCCTTCCGGTTTCGCGGTGGGCGCGGCGAGGGCGCGGTCTTTTTGCTCCAACAACCATTTCATCCACTTAACGGGTTGATTGCAACAGCCCACTTCAATAAGGCTGCCGACGATGTTACGCACCATGTGATGCACAAAAGCGTTAGCTTGAATATCCACGATAATGTACTGCCCCTGACGAGTGACGTTTAAATGATGGACGTTGCGCCATGGGGTGTTGGATTGGCATTGAGCGGCGCGGAAg                                                                                                         <  1:38161/250‑1 (MQ=255)                                                                                                                                       tCCGGTTTCGCGGTGGGCGCGGCGAGGGCGCGGTCTTTTTGCTCCAACAACCATTTCATCCACTTAACGGGTTGATTGCAACAGCCCACTTCAATAAGGCTGCCGACGATGTTACGCACCATGTGATGCACAAAAGCGTTAGCTTGAATATCCACGATAATGTACTGCCCCTGACGAGTGACGTTTAAATGATGGACGTTGCGCCATGGGGTGTTGGATTGGCATTGAGCGGCGCGGAAGGAGCTGAAATc                                                                                              <  1:740363/251‑1 (MQ=255)                                                                                                                                                    tGGGCGCGGCGAGGGCGCGGTCTTTTTGCTCCAACAACCATTTCATCCACTTAACGGGTTGATTGCAACAGCCCACTTCAATAAGGCTGCCGACGATGTTACGCACCATGTGATGCACAAAAGCGTTAGCTTGAATATCCACGATAATGTACTGCCCCTGACGAGTGACGTTTAAATGATGGACGTTGCGCCATGGGGTGTTGGATTGGCATTGAGCGGCGCGGAAGGAGCTGAAATCATTTTCGCCGagc                                                                                 <  1:380349/251‑1 (MQ=255)                                                                                                                                                          cggcgAGGGCGCGGTCTTTTTGCTCCAACAACCATTTCATCCACTTAACGGGTTGATTGCAACAGCCCACTTCAATAAGGCTGCCGACGATGTTACGCACCATGTGATGCACAAAAGCGTTAGCTTGAATATCCACGATAATGTACTGCCCCTGACGAGTGACGTTTAAATGATGGACGTTGCGCCATGGGGTGTTGGATTGGCATTGAGCGGCGCGGAAGGAGCTGAAATCATTTTCGCCGAGCAGCGcc                                                                           <  1:401969/251‑1 (MQ=255)                                                                                                                                                                     cgGTCTTTTTGCTCCAACAACCATTTCATCCACTTAACGGGTTGATTGCAACAGCCCACTTCAATAAGGCTGCCGACGATGTTACGCACCATGTGATGCACAAAAGCGTTAGCTTGAATATCCACGATAATGTACTGCCCCTGACGAGTGACGTTTAAATGATGGACGTTGCGCCATGGGGTGTTGGATTGGCATTGAGCGGCGCGGAAGGAGCTGAAATCATTTTCGCCGAGCAGCGCCTGTCCCGcgt                                                                 <  1:642268/250‑1 (MQ=255)                                                                                                                                                                              tGCTCCAACAACCATTTCATCCACTTAACGGGTTGATTGCAACAGCCCACTTCAATAAGGCTGCCGACGATGTTACGCACCATGTGATGCACAAAAGCGTTAGCTTGAATATCCACGATAATGTACTGCCCCTGACGAGTGACGTTTAAATGATGGACGTTGCGCCATGGGGTGTTGGATTGGCATTGAGCGGCGCGGAAGGAGCTGAAATCATTTTCGCCGAGCAGCGCCTGTCCCGCGTCGTGCATTaa                                                       <  1:179502/251‑1 (MQ=255)                                                                                                                                                                                cTCCAACAACCATTTCATCCACTTAACGGGTTGATTGCAACAGCCCACTTCAATAAGGCTGCCGACGATGTTACGCACCATGTGATGCACAAAAGCGTTAGCTTGAATATCCACGATAATGTACTGCCCCTGACGAGTGACGTTTAAATGATGGACGTTGCGCCATGGGGTGTTGGATTGGCATTGAGCGGCGCGGAAGGAGCTGAAATCATTTTCGCCGAGCAGCGCCTGTCCCGCGTCGTGCATTAAt                                                      <  1:26395/250‑1 (MQ=255)                                                                                                                                                                                             ttCATCCACTTAACGGGTTGATTGCAACAGCCCACTTCAATAAGGCTGCCGACGATGTTACGCACCATGTGATGCACAAAAGCGTTAGCTTGAATATCCACGATAATGTACTGCCCCTGACGAGTGACGTTTAAATGATGGACGTTGCGCCAt                                                                                                                                          <  1:292586/153‑1 (MQ=255)                                                                                                                                                                                                             gTTGATTGCAACAGCCCACTTCAATAAGGCTGCCGACGATGTTACGCACCATGTGATGCACAAAAGCGTTAGCTTGAATATCCACGATAATGTACTGCCCCTGACGAGTGACGTTTAAATGATGGACGTTGCGCCATGGGGTGTTGGATTGGCATTGAGCGGCGCGGAAGGAGCTGAAATCATTTTCGCCGAGCAGCGCCTGTCCCGCGTCGTGCATTAATTGATGATCGAGTTCTAAATGGCAATGGGt                         <  1:784767/250‑1 (MQ=255)                                                                                                                                                                                                                 aTTGCAACAGCCCACTTCAATAAGGCTGCCGACGATGTTACGCACCATGTGATGCACAAAAGCGTTAGCTTGAATATCCACGATAATGTACTGCCCCTGACGAGTGACGTTTAAATGATGGACGTTGCGCCATGGGGTGTTGGATTGGCATTGAGCGGCGCGGAAGGAGCTGAAATCATTTTCGCCGAGCAGCGCCTGTCCCGCGTCGTGCATTAATTGATGATCGAGTTCTAAATGGCAATGGGTTACGc                    <  1:459042/251‑1 (MQ=255)                                                                                                                                                                                                                 aTTGCAACAGCCCACTTCAATAAGGCTGCCGACGATGTTACGCACCATGTGATGCACAAAAGCGTTAGCTTGAATATCCACGATAATGTACTGCCCCTGACGAGTGACGTTTAAATGATGGACGTTGCGCCATGGGGTGTTGGATTGGCATTGAGCGGCGCGGAAGGAGCTGAAATCATTTTCGCCGAGCAGCGCCTGTCCCGCGTCGTGCATTAATTGATGATCGAGTTCTAAATGGCAATGGGTTACGc                    <  1:267514/251‑1 (MQ=255)                                                                                                                                                                                                                   tGCAACAGCCCACTTCAATAAGGCTGCCGACGATGTTACGCACCATGTGATGCACAAAAGCGTTAGCTTGAATATCCACGATAATGTGCTGCCCCTGACGAGTGACGTTTAAATGATGGACGTTGCGCCATGGGGTGTTGGATTGGCATTGAGCGGCGCGGAAGGGGCTGAGATCATTTTCGCCGAGCAGCGCCTGTCCCGCGTCGTGCATTAATTGATGATCGAGTTCTAAATGGCAATGGGTTACGCCt                  <  1:391475/251‑1 (MQ=255)                                                                                                                                                                                                                                 tCAATAAGGCTGCCGACGATGTTACGCACCATGTGATGCACAAAAGCGTTAGCTTGAATATCCACGATAATGTACTGCCCCTGACGAGTGACGTTTAAat                                                                                                                                                           <  1:634661/100‑1 (MQ=255)                                                                                                                                                                                                                                  cAATAAGGCTGCCGACGATGTTACGCACCATGTGATGCACAAAAGCGTTAGCTTGAATATCCACGATAATGTACTGCCCCTGACGAGTGACGTTTAAATGATGGACGTTGCGCCATGGGGTGTTGGATTGGCATTGAGCGGCGCGGAAGGAGCTGAAATc                                                                                              <  1:136127/160‑1 (MQ=255)                                                                                                                                                                                                                                  cAATAAGGCTGCCGACGATGTTACGCACCATGTGATGCACAAAAGCGTTAGCTTGAATATCCACGATAATGTACTGCCCCTGACGAGTGACGTTTAAATGATGGACGTTGCGCCATGGGGTGTTGGATTGGCATTGAGCGGCGCGGAAGGAGCTGAAATCATTTTCGCCGAGCAGCGCCTGTCCCGCGTCGTGCATTAATtga                                                   <  1:585538/203‑1 (MQ=255)                                                                                                                                                                                                                                   aaTAAGGCTGCCGACGATGTTACGCACCATGTGATGCACAAAAGCGTTAGCTTGAATATCCACGATAATGTACTGCCCCTGACGAGTGACGTTTAAATGATGGACGTTGCGCCATGGGGTGTTGGATTGGCATTGAGCGGCGCGGAAGGAGCTGAAATCATTTTCGCCGAGCAGCGCCTGTCCCGCGTCGTGCATTAATTGATGATCGAGTTCTAAATGGCAATGGGTTACGCCTTGCGGCAAAATCGCCg  <  1:293021/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                         AAATTTTGTTTATACCAAAGCCAAAAGCAGGCTTTAATCTATTCAATCATCAATCCGCCAGAAACAACGGTCCCATTGGCATTTTCGGCAGATTAAAGTTGTCGGGGTAAATGACATTCACCAGATACAACCCTTCCGGTTTCGCGGTGGGCGCGGCGAGGGCGCGGTCTTTTTGCTCCAACAACCATTTCATCCACTCAACGGGTTGATTGCAACAGCCCACTTCAATAAGGCTGCCGACGATATTACGCACCATGTGATGCACAAAAGCGTTAGCTTGAATATCCACGATAATGTACTGCCCCTGACGAGTGACGTTTAAATGATGGACGTTGCGCCATGGGGTGTTGGATTGGCATTGAGCGGCGCGGAAGGAGCTGAAATCATTTTCGCCGAGCAGCGCCTGTCCCGCGTCGTGCATTAATTGATGATCGAGTTCTAAATGGCAATGGGTTACGCCTTGCGGCAAAATCGCCG  >  1/1384392‑1384868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑