Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,375,892	C→A	intergenic (+236762/+17419)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,375,892	0	C	A	100.0%	191.1 / NA	67	intergenic (+236762/+17419)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (31/36);  total (31/36)

GTAATGATTGAAATAAAAAAGTGCGGTCGGTTTTCCCAACGTTTTTTCATCGTGAAAACCCACCGCACTTTTGATTCGAGGAAGGCATTAGGAAAGTATCTTTTTGAACACCTTCACATTATTAAATCCACTCTCTTTCAAATACAGCGCCTGCAATTTGCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAACGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGACGGAAACCACCTCTTTCTCCGTCTGCTCGGCGATTTGACGAATATCCAAATATTGCGCATTTTCCACCGCACTTTCCAACACGGCAAAATTGAAATTGCCTTCTTCCTGCTCGATTTTCGCTTTGAT  >  1/1375652‑1376131                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                gTAATGATTGAAATAAAAAAGTGCGGTCGGTTTTCCCAACGTTTTTTCATCGTGAAAACCCACCGCACTTTTGATTCGAGGAAGGCATTAGGAAAGTATCTTTTTGAACACCTTCACATTATTAAATCCACTCTCTTTCAAATACAGCGCCTGCAATTTGCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACaaaa                                                                                                                                                                                                                                       >  1:689831/1‑251 (MQ=255)                   aaGTGCGGTCGGTTTTCCCAACGTTTTTTCATCGTGAAAACCCACCGCACTTTTGATTCGAGGAAGGCATTAGGAAAGTATCTTTTTGAACACCTTCACATTATTAAATCCACTCTCTTTCAAATACAGCGCCTGCAATTTGCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCaa                                                                                                                                                                                                                     <  1:162231/251‑1 (MQ=255)                        cggtcggtTTTCCCAACGTTTTTTCATCGTGAAAACCCACCGCACTTTTGATTCGAGGAAGGCATTAGGAAAGTATCTTTTTGAACACCTTCACATTATTAAATCCACTCTCTTTCAAATACAGCGCCTGCAATTTGCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGt                                                                                                                                                                                                                <  1:245251/251‑1 (MQ=255)                                  tCCCAACGTTTTTTCATCGTGAAAACCCACCGCACTTTTGATTCGAGGAAGGCATTAGGAAAGTATCTTTTTGAACACCTTCACATTATTAAATCCACTCTCTTTCAAATACAGCGCCTGCAATTTGCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGttttt                                                                                                                                                                                                      >  1:750899/1‑251 (MQ=255)                                                     tGAAAACCCACCGCACTTTTGATTCGAGGAAGGCATTAGGAAAGTATCTTTTTGAACACCTTCACATTATTAAATCCACTCTCTTTCAAATACAGCGCCTGCAATTTGCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGc                                                                                                                                                                                    <  1:715807/250‑1 (MQ=255)                                                                            tCGAGGAAGGCATTAGGAAAGTATCTTTTTGAACACCTTCACATTATTAAATCCACTCTCTTTCAAATACAGCGCCTGCAATTTGCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCAt                                                                                                                                                            <  1:480819/251‑1 (MQ=255)                                                                                     gCATTAGGAAAGTATCTTTTTGAACACCTTCACATTATTAAATCCACTCTCTTTCAAATACAGCGCCTGCAATTTGCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCa                                                                                                                                                   >  1:46286/1‑251 (MQ=255)                                                                                            gAAAGTATCTTTTTGAACACCTTCACATTATTAAATCCACTCTCTTTCAAATACAGCGCCTGCAATTTGCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGc                                                                                                                                                                                    >  1:562469/1‑211 (MQ=255)                                                                                                gTATCTTTTTGAACACCTTCACATTATTAAATCCACTCTCTTTCAAATACAGCGCCTGCAATTTGCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGc                                                                                                                                                                                    >  1:691737/1‑207 (MQ=255)                                                                                                 tATCTTTTTGAACACCTTCACATTATTAAATCCACTCTCTTTCAAATACAGCGCCTGCAATTTGCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATgg                                                                                                                                       <  1:113446/251‑1 (MQ=255)                                                                                                        ttGAACACCTTCACATTATTAAATCCACTCTCTTTCAAATACAGCGCCTGCAATTTGCTCATCACGCCGCGTTCGCAATACGGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGAc                                                                                                                                <  1:83716/251‑1 (MQ=255)                                                                                                         tGAACACCTTCACATTATTAAATCCACTCTCTTTCAAATACAGCGCCTGCAATTTGCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGACg                                                                                                                               <  1:298600/251‑1 (MQ=255)                                                                                                               ccTTCACATTATTAAATCCACTCTCTTTCAAATACAGCGCCTGCAATTTGCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGc                                                                                                                                                 <  1:577443/227‑1 (MQ=255)                                                                                                               ccTTCACATTATTAAATCCACTCTCTTTCAAATACAGCGCCTGCAATTTGCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGACGGAAac                                                                                                                          >  1:589423/1‑250 (MQ=255)                                                                                                               ccTTCACATTATTAAATCCACTCTCTTTCAAATACAGCGCCTGCAATTTGCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGACGGAAac                                                                                                                          >  1:785366/1‑250 (MQ=255)                                                                                                               ccTTCACATTATTAAATCCACTCTCTTTCAAATACAGCGCCTGCAATTTGCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGACGGAAac                                                                                                                          >  1:135388/1‑250 (MQ=255)                                                                                                                  tCACATTATTAAATCCACTCTCTTTCAAATACAGCGCCTGCAATTTGCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGACGGAAaccacc                                                                                                                      <  1:54920/251‑1 (MQ=255)                                                                                                                     caTTATTAAATCCACTCTCTTTCAAATACAGCGCCTGCAATTTGCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCAc                                                                                                                                                                 >  1:505524/1‑205 (MQ=255)                                                                                                                         attaAATCCACTCTCTTTCAAATACAGCGCCTGCAATTTGCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCaa                                                                                                                                                                                                                     >  1:479199/1‑149 (MQ=255)                                                                                                                         attaAATCCACTCTCTTTCAAATACAGCGCCTGCAATTTGCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCgat                                                                                                                                                                       <  1:698663/195‑1 (MQ=255)                                                                                                                         attaAATCCACTCTCTTTCAAATACAGCGCCTGCAATTTGCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACg                                                                                                                                                                >  1:836790/1‑202 (MQ=255)                                                                                                                         attaAATCCACTCTCTTTCAAATACAGCGCCTGCAATTTGCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGACGGAAACCACCTCttt                                                                                                                 >  1:235821/1‑249 (MQ=255)                                                                                                                         attaAATCCACTCTCTTTCAAATACAGCGCCTGCAATTTGCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGACGGAAACCACCTCTTtct                                                                                                               <  1:173945/251‑1 (MQ=255)                                                                                                                          ttaAATCCACTCTCTTTCAAATACAGCGCCTGCAATTTGCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGACGGAAACCACCTCTTtctc                                                                                                              <  1:584413/251‑1 (MQ=255)                                                                                                                                     ctctTTCAAATACAGCGCCTGCAATTTGCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGAc                                                                                                                                <  1:801659/222‑1 (MQ=255)                                                                                                                                       ctTTCAAATACAGCGCCTGCAATTTGCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTa                                                                                                                                     <  1:630237/215‑1 (MQ=255)                                                                                                                                         ttCAAATACAGCGCCTGTAATTTGCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGACGGAAACCACCTCTTTCTCCGTCTGCTCGGCGAt                                                                                               >  1:508112/1‑251 (MQ=255)                                                                                                                                         ttCAAATACAGCGCCTGCAATTTGCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGc                                                                                                                                                                                                             >  1:295531/1‑141 (MQ=255)                                                                                                                                         ttCAAATACAGCGCCTGCAATTTGCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTa                                                                                                                                     >  1:162919/1‑213 (MQ=255)                                                                                                                                         ttCAAATACAGCGCCTGCAATTTGCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGACGGAAACCACCTCTTTCTCCGTCTGCTCGGCGAt                                                                                               >  1:340778/1‑251 (MQ=255)                                                                                                                                         ttCAAATACAGCGCCTGCAATTTGCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGACGGAAACCACCTCTTTCTCCGTCTGCTCGGCGAt                                                                                               >  1:738295/1‑251 (MQ=255)                                                                                                                                         ttCAAATACAGCGCCTGCAATTTGCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGACGGAAACCACCTCTTTCTCCGTCTGCTCGGCGAt                                                                                               >  1:411965/1‑251 (MQ=255)                                                                                                                                         ttCAAATACAGCGCCTGCAATTTGCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGACGGAAACCACCTCTTTCTCCGTCTGCTCGGCGAt                                                                                               >  1:287719/1‑251 (MQ=255)                                                                                                                                                    cgcCTGCAATTTGCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTtcctcc                                                                                                                                                                                        <  1:59699/151‑1 (MQ=255)                                                                                                                                                          cAATTTGCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGACGGAAACCACCTCTTTCTCCGTCTGCTCGGCGATTTGACGAATATCCAAat                                                                              <  1:382880/251‑1 (MQ=255)                                                                                                                                                          cAATTTGCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGACGGAAACCACCTCTTTCTCCGTCTGCTCGGCGATTTGACGAATATCCAAat                                                                              <  1:412205/251‑1 (MQ=255)                                                                                                                                                            aTTTGCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGACGGAAACCACCTCTTTCTCCGTCTGCTCGGCGATTTGACGAATATCCAAatat                                                                            <  1:297512/251‑1 (MQ=255)                                                                                                                                                            aTTTGCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGACGGAAACCACCTCTTTCTCCGTCTGCTCGGCGATTTGACGAATATCCAAatat                                                                            <  1:803381/251‑1 (MQ=255)                                                                                                                                                            aTTTGCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGACGGAAACCACCTCTTTCTCCGTCTGCTCGGCGATTTGACGAATATCCAAatat                                                                            <  1:783636/251‑1 (MQ=255)                                                                                                                                                            aTTTGCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGACGGAAACCACCTCTTTCTCCGTCTGCTCGGCGATTTGACGAATATCCAAatat                                                                            <  1:297947/251‑1 (MQ=255)                                                                                                                                                            aTTTGCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGACGGAAACCACCTCTTTCTCCGTCTGCTCGGCGATTTGACGAATATCCAAatat                                                                            <  1:605959/251‑1 (MQ=255)                                                                                                                                                                gCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGATGCCCAGCACGGaaa                                                                                                                                          >  1:454250/1‑185 (MQ=255)                                                                                                                                                                 cTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGACGGAAACCACCTCTTTCTCCGTCTGCTCGGCGATTTGACGAATATCCAAATATTgcgc                                                                       <  1:281526/251‑1 (MQ=255)                                                                                                                                                                               ttCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGACGGAAACCACCTCTTTCTCCGTCTGCTCGGCGATTTGACGAATATCCAAATATTGCGCATTTTCCACCGCAc                                                         <  1:740305/251‑1 (MQ=255)                                                                                                                                                                                tCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGACGGAAACCACCTCTTTCTCCGTCTGCTCGGCGATTTGACGAATATCCAAatat                                                                            >  1:259891/1‑231 (MQ=255)                                                                                                                                                                                tCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGAATCGACGGAAACCACCTCTTTCTCCGTCTGCTCGGCGATTTGACGAATATCCAAATATTGCGCATTTTCCACCGCa                                                          >  1:610928/1‑249 (MQ=255)                                                                                                                                                                                   cAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGATTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGACGGAAACCACCTCTTTCTCCGTCTGCTCGGCGATTTGACGAATATCCAAATATTGCGCATTTTCCACCGCACttt                                                      >  1:567235/1‑250 (MQ=255)                                                                                                                                                                                     aTACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGa                                                                                                                                            >  1:413759/1‑162 (MQ=255)                                                                                                                                                                                     aTACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGACGGAAACCACCTCTTTCTCCGTCTGCTCGGCGATTTGACGAATATCCAAatat                                                                            <  1:781983/226‑1 (MQ=255)                                                                                                                                                                                       aCAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTGAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGACGGAAACCGCCTCTTTCTCCGTCTGCTCGGCGATTTGACGAATATCCAAatat                                                                            <  1:443633/224‑1 (MQ=255)                                                                                                                                                                                          gCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGaaa                                                                                                                                          <  1:635090/159‑1 (MQ=255)                                                                                                                                                                                          gCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGACGGAAACCACCTCTTTCTCCGTCTGCTCGGCGATTTGACGAATATCCAAATATTGCGCATTTTCCACCGCACTTTCCAacac                                               >  1:353302/1‑250 (MQ=255)                                                                                                                                                                                           cacaTAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGACGGAAACCACCTCTTTCTCCGTCTGCTCGGCGATTTGACGAATATCCAAATATTGCGCATTTTCCACCGCACTTTCCAacac                                               <  1:753549/249‑1 (MQ=255)                                                                                                                                                                                           cacaTAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGACGGAAACCACCTCTTTCTCCGTCTGCTCGGCGATTTGACGAATATCCAAATATTGCGCATTTTCCACCGCACTTTCCAacac                                               <  1:644009/249‑1 (MQ=255)                                                                                                                                                                                           cacaTAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGACGGAAACCACCTCTTTCTCCGTCTGCTCGGCGATTTGACGAATATCCAAATATTGCGCATTTTCCACCGCACTTTCCAac                                                 <  1:171972/247‑1 (MQ=255)                                                                                                                                                                                                 gTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAg                                                                                                                                                  <  1:339502/144‑1 (MQ=255)                                                                                                                                                                                                  tttttGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGACGGAAACCACCTCTTTCTCCGTCTGCTCGGCGATTTGACGAATATCCAAATATTGCGCATTTTCCACCGCACTTTCCAACACGGCAaaat                                       <  1:202144/250‑1 (MQ=255)                                                                                                                                                                                                  tttttGCTTTGATCCAGTTCGGCAAAGTGATTGGACAGAGTGAAAAAAGGCAAAAAAATCATCGTAGGCTTGTCAATATGTAGCGGTTTTTCGGCGAGGACCTCCGGGATGCGGATGTCGAACATCAAGACATTGATGCCCAGCAAGGACATGGTATAGACGGAAACCACAGATTTCTCCGTCTGATCGGCGATTTGACGAATATCCAAATATTGCGCATTTTCCACCGCACTATCCAACACGGCAatatt                                      >  1:725796/1‑251 (MQ=255)                                                                                                                                                                                                       gCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGACGGAAACCACCTCTTTCTCCGTCTGCTCGGCGATTTGACGAATATCCAAATATTg                                                                          <  1:769852/210‑1 (MQ=255)                                                                                                                                                                                                            gATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGACGGAAACCACCTCTTTCTCCGTCTGCTCGGCGATTTGACGAATATCCAAATATTGCGCAtttt                                                                  <  1:81426/213‑1 (MQ=255)                                                                                                                                                                                                             aTCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGACGGAAACCACCTCTTTCTCCGTCTGCTCGGCGATTTGACGAATATCCAAATATTGCGCATTTTCCACCGCACTTTCCAACACGGCAAAATTGAAATTGCctt                           >  1:120360/1‑251 (MQ=255)                                                                                                                                                                                                                      ggCAAATTGATTGGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGACGGAAACCACCTCTTTCTCCGTCTGCTCGGCGATTTGACGAATATCCAAATATTGCGCATTTTCCACCGCACTTTCCAACACGGCAAAATTGAAATTGCCTTCTTCCTg                    >  1:229202/1‑249 (MQ=255)                                                                                                                                                                                                                            ttgattgGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGACGGAAACCACCTCTTtctc                                                                                                              >  1:848277/1‑153 (MQ=255)                                                                                                                                                                                                                              gattgGACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGACGGAAACCACCTCTTTCTCCGTCTGCTCGGCGATTTGACGAATATCCAAATATTGCGCATTTTCCACCGCACTTTCCAACACGGCAAAATTGAAATTGCCTTCTTCCTGCTCGAtttt           >  1:818451/1‑250 (MQ=255)                                                                                                                                                                                                                                   gACAGTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGACGGAAACCACCTCTTTCTCCGTCTGCTCGGCGATTTGACGAATATCCAAATATTGCGCATTTTCCACCGCACTTTCCAACACGGCAAAATTGAAATTGCCTTCTTCCTGCTCGATTTTCGCttt     >  1:162091/1‑251 (MQ=255)                                                                                                                                                                                                                                       gTTTGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGACGGAAACCACCTCTTTCTCCGTCTGCTCGGCGATTTGACGAATATCCAAATATTGCGCATTTTCCACCGCACTTTCCAacac                                               <  1:344842/205‑1 (MQ=255)                                                                                                                                                                                                                                        tttGTAAAAAGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGGACGGAAATGGTATCGACGGAAACCACCTCTTTCTCCGTCTGCTCGGCGATTTGACGAATATCCAAATATTGCGCATTTTCCACCGCACTTTCCAACACGGCAAAATTGAAATTGCCTTCTTCCTGCTCGATTTTCGCTTTGAt  <  1:896547/249‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                GTAATGATTGAAATAAAAAAGTGCGGTCGGTTTTCCCAACGTTTTTTCATCGTGAAAACCCACCGCACTTTTGATTCGAGGAAGGCATTAGGAAAGTATCTTTTTGAACACCTTCACATTATTAAATCCACTCTCTTTCAAATACAGCGCCTGCAATTTGCTCATCACGCCGCGTTCGCAATACAGCACATAGTTTTTGCTTTGATCCAGTTCGGCAAATTGATTGGACAGTTTGTAAAACGGCAACAAAATCATCGTTTGCTTTTCAATATGTAGCGGTTTTTCGTCGATTTCCTCCGGGCTGCGGATGTCGATGATCACGTCATTGGTGCCCAGCACGGAAATGGTATCGACGGAAACCACCTCTTTCTCCGTCTGCTCGGCGATTTGACGAATATCCAAATATTGCGCATTTTCCACCGCACTTTCCAACACGGCAAAATTGAAATTGCCTTCTTCCTGCTCGATTTTCGCTTTGAT  >  1/1375652‑1376131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑