Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,373,736	T→G	intergenic (+234606/+19575)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,373,736	0	T	G	100.0%	172.2 / NA	57	intergenic (+234606/+19575)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (24/33);  total (24/33)

CACCATCGGCAAACCGATCATTGATGCCGCCATTGTTCCTTTTGACAACGCACCGGAAAATCTGCACGACATCAAAATCCTCGCCGGTCGCCTCAACGCCGACGACATCACCTACCGCCACGACCGCGCCCTCCTCTGCTCCGCCATTGACGAGCTATTGGGCGAACTGCTGGAATCGCACGACGACGAAGAACATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAATAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGTTCTTTCTGTTCAGGCAAACTTTCCGCCACAAGCAATGCTAATGCCACTAAAGTATTGTCATTAATCAATTGTTCTACCGGCCTTGCCAATAAGTGTTGATTCAACCGCAAATACCATAAAAACAGGAAAGATCCCGTGCGTTTATTGCCATCGGT  >  1/1373495‑1373976                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 cacCATCGGAAAACCGATCATTGATGCCGCCATTGTTCCTTTTGACAACGCCCCGGAAAATCTGCACGACATCAAAATCCTCGCCGGTCGCCTCAACGCCGACGACATCCCCTACCGCCACGACCGCGCCCTCCTCTGCTCCGCCATTGACGAGCTATTGGGCGAACTGCTGGAATCGCACGACGACGAAGAACATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCAc                                                                                                                                                                                                                                         <  1:240997/251‑1 (MQ=255)             cccGATCATTGATGCCGCCATTGTTCCTTTTGACACCGCCCCGGAAAATCTGCACGACATCAAAATCCTCGCCGGTCGCCTTACCGCCGACGACATCACCTACCGCCACGACCGCGCCCTCCTCTGCTCCGCCATTGACGAGCTATTGGGCGAACTGCTGGAATCGCACGACGACGAAGAACATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTtttatt                                                                                                                                                                                                                             <  1:445052/250‑1 (MQ=255)             cccGATCATGGATGCCGCCATTGTCCCTTTTGACAACGCCCCGGAAAATCTGCACGACATCAAAACCCTCGCCGGTCGCCTCAACGCCGACGACATCACCTACCGCCACGACCGCGCCCTCCTCTGCTCCGCCATTGACGAGCTATTGGGCGAACTGCTGGAATCGCACGACGACGAAGAACATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTtttatt                                                                                                                                                                                                                             <  1:287571/250‑1 (MQ=255)             aCCGATCATTGATGCCGCCATTGTTCCTTTTGACAACGCACCGGAAAATCTGCACGACATCAAAATCCTCGCCGGTCGCCTCAACGCCGACGACATCACCTACCGCCACGACCGCGCCCTCCTCTGCTCCGCCATTGACGAGCTATTGGGCGAACTGCTGGAATCGCACGACGACGAAGAACATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTtttatt                                                                                                                                                                                                                             >  1:520853/1‑251 (MQ=255)                gATCATTGATGCCGCCATTGTTCCTTTTGACAACGCACCGGAAAATCTGCACGACATCAAAATCCTCGCCGGTCGCCTCAACGCCGACGACATCACCTACCGCCACGACCGCGCCCTCCTCTGCTCCGCCATTGACGAGCTATTGGGCGAACTGCTGGAATCGCACGACGACGAAGAACATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTttatttat                                                                                                                                                                                                                          >  1:643728/1‑251 (MQ=255)                                    ttCCTTTTGACAACGCACCGGAAAATCTGCACGACATCAAAATCCTCGCCGGTCGCCTCAACGCCGACGACATCCCCTACCGCCACGACCGCGCCCTCCTCTGCTCCGCCATTGACGAGCTATTGGGCGAACTGCTGGAATCGCACGACGACGAAGAACATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAg                                                                                                                                                                                                       <  1:652152/250‑1 (MQ=255)                                      ccTTTTGACAACGCACCGGAAAATCTGCACGACATCAAAATCCTCGCCGGTCGCCTCAACGCCGACGACATCACCTACCGCCACGACCGCGCCCTCCTCTGCTCCGCCATTGACGAGCTATTGGGCGAACTGCTGGAATCGCACGACGACGAAGAACATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGttt                                                                                                                                                                                                    >  1:376814/1‑251 (MQ=255)                                                     ccGGAAAATCTGCACGACATCAAAACCCTCGCCGGTCGCCCCAACGCCGACGACATCACCTACCGCCACGACCGCGCCCTCCTCTGCTCCGCCATTGACGAGCTATTGGGCGAACTGCTGGAATCGCACGACGACGAAGAACATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCaaaataaaa                                                                                                                                                                                     <  1:884717/251‑1 (MQ=255)                                                        aaaaaTCTGCACGACATCAAAATCCTCGCCGGTCGCCTCAACGCCGACGACATCACCTACCGCCACGACCGCGCCCTCCTCTGCTCCGCCATTGACGAGCTATTGGGCGAACTGCTGGAATCGCACGACGACGAAGAACATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGc                                                                                                                                                                                  <  1:364735/250‑1 (MQ=255)                                                                     aCATCAAAATCCTCGCCGGTCGCCTCAACGCCGACGACATCACCTACCGCCACGACCGCGCCCTCCTCTGCTCCGCCATTGACGAGCTATTGGGCGAACTGCTGGAATCGCACGACGACGAAGAACATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTaa                                                                                                                                                                     >  1:864540/1‑251 (MQ=255)                                                                                   cccGGTCGCCTCAACGCCGACGACATCACCTACCGCCACGACCGCGCCCTCCTCTGCTCCGCCATTGACGAGCTATTGGGCGAACTGCTGGAATCGCACGACGACGAAGAACATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGttctttc                                                                                                                                                       <  1:230915/250‑1 (MQ=255)                                                                                             ccaccGCCGACGACATCCCCTACCGCCACGACCGCGCCCTCCTCTGCTCCGCCATTGACGAGCTATTGGGCGAACTGCTGGAATCGCACGACGACGAAGAACATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGTTCTTTCTGTTCAGGCa                                                                                                                                             <  1:867893/247‑1 (MQ=255)                                                                                                             cACCTACCGCCACGACCGCGCCCTCCTCTGCTCCGCCATTGACGAGCTATTGGGCGAACTGCTGGAATCGCACGACGACGAAGAACATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGTTCTTTCTGTTCAGGCAAACTTTCCGCCACAAg                                                                                                                             >  1:271362/1‑251 (MQ=255)                                                                                                               ccTACCGCCACGACCGCGCCCTCCTCTGCTCCGCCATTGACGAGCTATTGGGCGAACTGCTGGAATCGCACGACGACGAAGAACATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGTTCTTTCTGTTCAGGCAAACTTTCCGCCACAAGCa                                                                                                                           >  1:134154/1‑251 (MQ=255)                                                                                                                    cGCCACGACCGCGCCCTCCTCTGCTCCGCCATTGACGAGCTATTGGGCGAACTGCTGGAATCGCACGACGACGAAGAACATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGTTCTTTCTGTTCAGGCAAACTTTCCGCCACAAGCAATg                                                                                                                        >  1:189058/1‑249 (MQ=255)                                                                                                                        ccGACCGCGCCCTCCTCTGCTCCGCCATTGACGAGCTATTGGGCGAACTGCTGGAATCGCACGACGACGAAGAACATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGTTCTTTCTGTTCAGGCAAACTTTCCGCCACAAGCAATGCTAATg                                                                                                                  <  1:406916/250‑1 (MQ=255)                                                                                                                         cGACCGCGCCCTCCTCTGCTCCGCCATTGACGAGCTATTGGGCGAACTGCTGGAATCGCACGACGACGAAGAACATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGTTCTTTCTGTTCAGGCAAACTTTCCGCCACAAGCAATGCTAATGc                                                                                                                 <  1:896972/251‑1 (MQ=255)                                                                                                                             cgcgCCCTCCTCAGCTCCGCCATTGACGAGCTATTGGGCGAACTGCTGGAATCGCACGACGACGAAGAACATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGTTCTTTCTGTTCAGGCAAACTTTCCGCCACAAGCAATGCTAATGCCACt                                                                                                             <  1:322098/251‑1 (MQ=255)                                                                                                                               cgcCCTCCTCTGCTCCGCCATTGACGAGCTATTGGGCGAACTGCTGGAATCGCACGACGACGAAGAACATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGTTCTTTCTGTTCAGGCAAACTTTCCGCCACAAGCAATGCTAATGCCACTaa                                                                                                           >  1:601271/1‑251 (MQ=255)                                                                                                                                 cccTCCTCTGCTCCGCCATTGACGAGCTATTGGGCGAACTGCTGGAATCGCACGACGACGAAGAACATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGTTCTTTCTGTTCAGGCAAACTTTCCGCCACAAGCAATGCTAATGCCACTAAAg                                                                                                         <  1:331416/251‑1 (MQ=255)                                                                                                                                 cccTCCTCTGCTCCGCCATTGACGAGCTATTGGGCGAACTGCTGGAATCGCACGACGACGAAGAACATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGTTCTTTCTGTTCAGGCAAACTTTCCGCCACAAGCAATGCTAATGCCACTAAAg                                                                                                         <  1:885211/251‑1 (MQ=255)                                                                                                                                   ctcctcTGCTCCGCCATTGACGAGCTATTGGGCGAACTGCTGGAATCGCACGACGACGAAGAACATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACt                                                                                                                                                                        >  1:413466/1‑186 (MQ=255)                                                                                                                                   ctcctcTGCTCCGCCATTGACGAGCTATTGGGCGAACTGCTGGAATCGCACGACGACGAAGAACATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCa                                                                                                                                                                  <  1:610871/192‑1 (MQ=255)                                                                                                                                   ctcctcTGCTCCGCCATTGACGAGCTATTGGGCGAACTGCTGGAATCGCACGACGACGAAGAACATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGTTCTTTCTGtt                                                                                                                                                   >  1:222189/1‑207 (MQ=255)                                                                                                                                   ctcctcTGCTCCGCCATTGACGAGCTATTGGGCGAACTGCTGGAATCGCACGACGACGAAGAACATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGTTCTTTCTGTTCAGGCa                                                                                                                                             <  1:489944/213‑1 (MQ=255)                                                                                                                                   ctcctcTGCTCCGCCATTGACGAGCTATTGGGCGAACTGCTGGAATCGCACGACGACGAAGAACATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGTTCTTTCTGTTCAGGCAAACTTTCCGCCACAAGCAATGCTAATGCCACTAAAGTa                                                                                                       >  1:16585/1‑251 (MQ=255)                                                                                                                                         tGCTCCGCCATTGACGAGCTATTGGGCGAACTGCTGGAATCGCACGACGACGAAGAACATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGTTCTTTCTGTTCAGGCAAACTTTCCGCCACAAGCAATGCTAATGCCACTAAAGTATTGTCa                                                                                                 <  1:465901/251‑1 (MQ=255)                                                                                                                                           cTCCGCCATTGACGAGCTATTGGGCGAACTGCTGGAATCGCACGACGACGAAGAACATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGTTCTTTCTGTTCAGGCAAACTTTCCGCCACAAGCAATGCTAATGCCACTAAAGTATTGTCAt                                                                                                <  1:306012/250‑1 (MQ=255)                                                                                                                                              cGCCATTGACGAGCTATTGGGCGAACTGCTGGAATCGCACGACGACGAAGAACATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGTTCTTTCTGTTCAGGCAAACTTTCCGCCACAAGCAATGCTAATGCCACTAAAGTATTGTCATTAAt                                                                                            <  1:416218/251‑1 (MQ=255)                                                                                                                                               gCCATTGACGAGCTATTGGGCGAACTGCTGGAATCGCACGACGACGAAGAACATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCtc                                                                                                                                                                                <  1:584117/166‑1 (MQ=255)                                                                                                                                                ccATTGACGAGCTATTGGGCGAACTGCTGAAATCGCACGACGACGAAGAACATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGTTCTTTCTGTTCAGGCAAACTTTCCGCCACAAGCAATGCTAATGCCACTAAAGTATTGTCATTAATc                                                                                           <  1:336242/250‑1 (MQ=255)                                                                                                                                                          gCTATTGGGCGAACTGCTGGAATCGCACGACGACGAAGAACATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTtttattt                                                                                                                                                                                                                            >  1:555594/1‑111 (MQ=255)                                                                                                                                                           cTATTGGGCGAACTGCTGGAATCGCACGACGACGAAGAACATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGTTCTTTCTGTTCAGGCAAACTTTCCGCCACAAGCAATGCTAATGCCACTAAAGTATTGTCATTAATCAATTGTTCTAc                                                                                <  1:543670/250‑1 (MQ=255)                                                                                                                                                           cTATTGGGCGAACTGCTGGAATCGCACGACGACGAAGAACATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGTTCTTTCTGTTCAGGCAAACTTTCCGCCACAAGCAATGCTAATGCCACTAAAGTATTGTCATTAATCAATTGTTCTAc                                                                                >  1:773176/1‑250 (MQ=255)                                                                                                                                                             aTTGGGCGAACTGCTGGAATCGCACGACGACGAAGAACATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGTTCTTTCTGTTCAGGCAAACTTTCCGCCACAAGCAATGCTAATGCCACTAAAGTATTGTCATTAATCAATTGTTCTACCgg                                                                             >  1:472908/1‑251 (MQ=255)                                                                                                                                                                gggCGAACTGCTGGAATCGCACGACGACGAAGAACATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGTTCTTTCTGTTCAGGCAAACTTTCCGCCACAAGCAATGCTAATGc                                                                                                                 >  1:602906/1‑212 (MQ=255)                                                                                                                                                                        tgctgGAATCGCACGACGACGAAGAACATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGTTCTTTCTGTTCAGGCAAACTTTCCGCCACAAGCAATGCTAATGCCACTAAAGTATTGTCATTAATc                                                                                           <  1:674685/226‑1 (MQ=255)                                                                                                                                                                          ctgGAATCGCACGACGACGAAGAACATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGTTCTTTCTGTTCAGGCAAACTTTCCGCCACAAGCAATGCTAATGCCACTAAAGAATTGTCATTAATCAATTGTTCTACCGGCCTTGCCAATAAg                                                                <  1:808713/251‑1 (MQ=255)                                                                                                                                                                                   cACGACGACGAAGAACATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGTTCTTTCTGTTCAGGCAAACTTTCCGCCACAAGCAATGCTAATGCCACTAAAGTATTGTCATTAATCAATTGTTCTACCGGCCTTGCCAATAAGTGTTGATTc                                                       <  1:62266/251‑1 (MQ=255)                                                                                                                                                                                       acgacgaAGAACATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGTTCTTTCTGTTCAGGCAAACTTTCCGCCACAAGCAATGCTAATGCCACTAAAGTATTGTCATTAATCAATTGTTCTACCGGCCTTGCCAATAAGTGTTGATTCAAcc                                                   <  1:148829/251‑1 (MQ=255)                                                                                                                                                                                               gaaCATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCt                                                                                                                                                                                                                       <  1:462189/79‑1 (MQ=255)                                                                                                                                                                                                  cATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGTTCTTTCTGTTCAGGCAAACTTTCCGCCACAAGCAATGCTAATGCCACTAAAGTATTGTCATTAATCAATTGTTCTACCGGCCTTGCCAATAAGTGTTGATTCAACCGCAAATACCAt                                        >  1:28710/1‑251 (MQ=255)                                                                                                                                                                                                            ccATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGttctttc                                                                                                                                                       <  1:661819/130‑1 (MQ=255)                                                                                                                                                                                                            ccATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGTTCTTTCTGTTCAGGCAAACTTTCCGCCACAAGCAATGCTAATGCCCCTAAAGTATTGTCATTAATCAATTGTTCTACCGGCCTTGCCAATAAGTGTTGATTCAACCGCAAATACCATAAAAACAGGa                              >  1:431767/1‑251 (MQ=255)                                                                                                                                                                                                            ccATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGTTCTTTCTGTTCAGGCAAACTTTCCGCCACAAGCAATGCTAATGCCACTAAAGTATTGTCATTAATCAATTGTTCTACCGGcc                                                                           >  1:722063/1‑206 (MQ=255)                                                                                                                                                                                                            ccATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGTTCTTTCTGTTCAGGCAAACTTTCCGCCACAAGCAATGCTAATGCCACTAAAGTATTGTCATTAATCAATTGTTCTACCGGCCTTGCCAATAAg                                                                <  1:541973/217‑1 (MQ=255)                                                                                                                                                                                                              aTTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGTTCTTTCTGTTCAGGCAAACTTTCCGCCACAAGCAATGCTAATGCCACt                                                                                                             <  1:331052/170‑1 (MQ=255)                                                                                                                                                                                                              aTTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGTTCTTTCTGTTCAGGCAAACTTTCCGCCACAAGCAATGCTAATGCCACTAAAGt                                                                                                        >  1:654115/1‑175 (MQ=255)                                                                                                                                                                                                              aTTAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGTTCTTTCTGTTCAGGCAAACTTTCCGCCACAAGCAATGCTAATGCCACTAAAGTATTGTCATTAATCAATTGTTCTACCGGCCTTGCCAATAAGTGTTGAtt                                                        <  1:737051/223‑1 (MQ=255)                                                                                                                                                                                                               ttAATATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGTTCTTTCTGTTCAGGCAAACTTTCCGCCACAAGCAATGCTAATGCCACTAAAGTATTGTCATTAATCAATTGTTCTACCGGCCTTGCCAATAAGTGTTGATTCAACCGCAAATACCATAAAAACAGGAAAg                           >  1:851157/1‑251 (MQ=255)                                                                                                                                                                                                                 aaTATCTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGTTCTTTCTGTTCAGGCAAACTTTCCGCCACAAGCAATGCTAATGCCACTAAAGTATTGTCATTAATCAATTGTTCTACCGGCCTTGCCAATAAGTGTTGATTCAACCGCAAATACCATAAAAACAGGAAAGAt                         <  1:124405/251‑1 (MQ=255)                                                                                                                                                                                                                    atCTAAAACAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGCTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGTTCTTTCTGTTCAGGCAAACTTTCCGCCACAAGCAATGCTAATGCCACTAAAg                                                                                                         >  1:373651/1‑168 (MQ=255)                                                                                                                                                                                                                      cTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGTTCTTTCTGTTCAGGCAAACTTTCCGCCACAAGCAATGc                                                                                                                       >  1:638914/1‑152 (MQ=255)                                                                                                                                                                                                                      cTAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGTTCTTTCTGTTCAGGCAAACTTTCCGCCACAAGCAATGCTAATGc                                                                                                                 >  1:570443/1‑158 (MQ=255)                                                                                                                                                                                                                       tAAAAAAGTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGTTCTTTCTGTTCAGGCAAACTTTCCGCCACAAGCAATGCTAATGCCACTAAAGTATTGTCATTAATCAATTGTTCTACCGGCCTTGCCAATAAGTGTTGATTCAACCGCAAATACCATAAAAACAGGAAAGATCCCGTg                   <  1:296922/251‑1 (MQ=255)                                                                                                                                                                                                                              gTGCGGTGAAAAAACACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGTTCTTTCTGTTCAGGCAAACTTTCCGCCACAAGCAATGCTAATGCCACTAAAGTATTGTCATTAATCAATTGTTCTACCGGCCTTGCCAATAAGTGTTGATTCAACCGCAAAt                                             >  1:563119/1‑218 (MQ=255)                                                                                                                                                                                                                                        aaaaCACAAAGAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGTTCTTTCTGTTCAGGCAAACTTTCCGCCACAAGCAATGCTAATGCCACTAAAGTATTGTCATTAATCAATTGTTCTACCGGCCTTGCCAATAAGTGTTGATTCAACCGCAAATACCATAAAAACAGGAAAGATCCCGTGCGTTTATTGCCATCGGt  <  1:433562/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 CACCATCGGCAAACCGATCATTGATGCCGCCATTGTTCCTTTTGACAACGCACCGGAAAATCTGCACGACATCAAAATCCTCGCCGGTCGCCTCAACGCCGACGACATCACCTACCGCCACGACCGCGCCCTCCTCTGCTCCGCCATTGACGAGCTATTGGGCGAACTGCTGGAATCGCACGACGACGAAGAACATTTGGAAGCCATTAATATCTAAAAAAGTGCGGTGAAAAAACACAAATAATTTTCACCGCACTTTTATTTATTCTAAAGTTTGAATGCTAGTTTTTTAGCAAAATAAAATGCTCAATCAACTTAATCATGAGTTCTTTCTGTTCAGGCAAACTTTCCGCCACAAGCAATGCTAATGCCACTAAAGTATTGTCATTAATCAATTGTTCTACCGGCCTTGCCAATAAGTGTTGATTCAACCGCAAATACCATAAAAACAGGAAAGATCCCGTGCGTTTATTGCCATCGGT  >  1/1373495‑1373976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑